FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60996
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA32895.392156862745098No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG16802.7542789691127285No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC12762.0919404551118106No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT12542.0558725162305724No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC10261.6820775132795593No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA9931.6279756049577023No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG9401.5410846612892648No Hit
TGTAAAACGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTCTGGCATGG8991.4738671388287756No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG8421.3804183880910224No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG8301.3607449668830742No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG8131.332874286838481No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA7271.1918814348481868No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT6931.1361400747590007No Hit
TGTAAAACGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT6881.1279428159223555No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT6741.1049904911797495No Hit
TGTAAAACGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT6541.0722014558331694No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG6181.0131811922093252No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG5960.9771132533280871No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA5630.9230113450062299No Hit
TGTAAAACGACGGCCAGTGTTCTACCGTCAGTCCTGGCATTATTTCTCTC5100.8361204013377926No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG4950.8115286248278576No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC4930.8082497212931996No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC4700.7705423306446324No Hit
TGTAAAACGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG4530.7426716506000394No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC4470.7328349399960653No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC4410.7229982293920914No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC4270.7000459046494852No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC3510.5754475703324808No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT3370.5524952455898747No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC3170.5197062102432947No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC3020.4951144337333595No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC2910.4770804642927405No Hit
TGTAAAACGACGGCCAGTAGTGACAACAGCCCCAAAGGAATGATCCCGCT2360.3869106170896452No Hit
TGTAAAACGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGGGACACGGA2300.3770739064856712No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGCTACGGGGT2270.37215555118368415No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA2170.3557610335103941No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCACTTTTGCCTCG2150.35248212997573614No Hit
TGTAAAACGACGGCCAGTCAGTTCCCAGGGACTCTGCTCTCAGATTCTGG2020.33116925700045907No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA2000.32789035346580103No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC1990.32625090169847204No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC1950.319693094629156No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCTCCTTGGCCTCC1860.304938028723195No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC1780.29182241458456293No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCAAGAGGTGGGGT1780.29182241458456293No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC1740.2852646075152469No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC1720.28198570398058886No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG1720.28198570398058886No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA1560.2557544757033248No Hit
TGTAAAACGACGGCCAGTCGCCTGAATAGGTGCAGGGGCACGAGCTCGGC1550.2541150239359958No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA1540.2524755721686668No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC1520.2491966686340088No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCATCAGGTTCCC1500.24591776509935076No Hit
TGTAAAACGACGGCCAGTCGCCTGAATAGGTGCAGGGGCACGATATCCAT1380.22624434389140274No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG1330.21804708505475767No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT1290.21148927798544168No Hit
TGTAAAACGACGGCCAGTGCCCAAGGTTGGTGAGATGGGGCCAGGTCATA1290.21148927798544168No Hit
TGTAAAACGACGGCCAGTTGACACAGCTGCTTCTGAGTACAAGTATGTAG1240.20329201914879663No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG1190.19509476031215162No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGTAAAACGACG1190.19509476031215162No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC1140.18689750147550657No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAAGCGCCAGACAG1130.18525804970817758No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC1080.17706079087153256No Hit
TGNAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAATCTCCACGCAGGA1070.17542133910420354No Hit
TGTAAAACGACGGCCAGTCAGTTCCCAGGGACTCTGCTCTATGAGAGAGA1060.17378188733687455No Hit
TGTAAAACGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG1000.16394517673290052No Hit
TGNAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAATAAGGAGACGATG960.1573873696635845No Hit
TGTAAAACGACGGCCAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA950.1557479178962555No Hit
TGTAAAACGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA930.1524690143615975No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC930.1524690143615975No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGTGAACGAAG870.14263230375762345No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG860.14099285199029443No Hit
TGTAAAACGACGGCTAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG850.13935340022296544No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTCATCCTGGGCAA840.13771394845563642No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTTGGACCCAGAG820.1344350449209784No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA820.1344350449209784No Hit
TGTAAAACGACGGCCAGTTCCTGGGCTTCTCTTGAGTACGGAGGAAAGTG820.1344350449209784No Hit
TGTAAAACGACGGCTAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA820.1344350449209784No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAACACCTCCACGCAGGA800.1311561413863204No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCCAAGAGGTGGGG790.1295166896189914No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAGCC790.1295166896189914No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG760.1245983343170044No Hit
TGTAAAACGACGGCCAGTCGCCTGAATAGGTGCAGGGGTGCAATCACAGC710.11640107548035936No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAAGGAAGGAGTAA680.11148272017837235No Hit
TGTAAAACGACGGCCAGTAAGAGGTGAGCGAGGTCTGCACCACCCCTGGC660.10820381664371434No Hit
TGTAAAACGACGGCCAGTTGGAGTGCCTGGGTTAGAACCCTGCTGGTACC630.10328546134172734No Hit
TGTAAAACGACGGCCAGTGAACAGCCCCGGAAGCAAGTAAAAGGAGGAGG630.10328546134172734No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCTCAACAAGGAGACGATG630.10328546134172734No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC620.10164600957439832No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)