FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118713
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG42613.589328885631734No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA40423.4048503533732615No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA32822.764650880695459No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC30482.567536832528872No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG28792.425176686630782No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCAAGGGT28592.408329332086629No Hit
TGTAAAACGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT27182.2895554825503526No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG26832.260072612098085No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT25022.1076040534735037No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC23812.0056775584813793No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA23641.9913573071188497No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA23611.9888302039372265No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC20551.7310656794116905No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC20231.7041099121410461No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC20161.6982133380505926No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC19611.651883113054173No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG19211.6181884039658674No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG17421.4674045807957006No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA16621.4000151626190898No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCTGTAAAACGACG15411.2980886676269658No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT14501.2214332044510712No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC14251.20037401127088No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCTGCTGTCCTCA14211.1970045403620497No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC13711.154886154001668No Hit
TGTAAAACGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC12341.0394817753742218No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG12061.015895479012408No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC11981.0091565371947468No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT11360.9569297381078735No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA10940.921550293565153No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT10680.8996487326577544No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCCAAGAGGTGGGG10670.8988063649305468No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCAAGAGGTGGGGT10520.8861708490224323No Hit
TGTAAAACGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT9560.8053035472104992No Hit
TGTAAAACGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGGGACACGGA9350.7876138249391389No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG9330.7859290894847236No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCATCAGGTTCCC8970.7556038513052488No Hit
TGTAAAACGACGGCCAGTAGGTCATCCTCCTGCCTCGGCCTCCCAAAGTG8850.7454954385787572No Hit
TGTAAAACGACGGCCAGTTCCTGGGCTTCTCTTGAGTACGGAGGAAAGTG8710.7337022903978503No Hit
TGTAAAACGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG8370.7050617876727907No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGCACATGGGT8190.6898991685830532No Hit
TGTAAAACGACGGCCAGTTGGAGTGCCTGGGTTAGAACCCTGCTGGTACC7770.6545197240403325No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC7710.6494655176770867No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC7630.6427265758594256No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGTGAACGAAG7050.5938692476813828No Hit
TGTAAAACGACGGCCAGTCCTGCCGCCTGGTCTCACCTCGCCATGGTTCG5720.4818343399627673No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCGCCTCGGCCTCC5690.47930723678114445No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT5460.4599327790553688No Hit
TGTAAAACGACGGCCAGTCCTGCCGCCTGGTCTCACCTCGCTATGGTTCG5230.4405583213295932No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGCATTTCCTTGGC5150.43381937951193217No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGAGGCTCCTTCC4930.4152872895133642No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG4520.38075021269785114No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCGGCTTACT4230.3563215486088297No Hit
TGTAAAACGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCATTTATT4100.34537076815513046No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCTGGAGGGCAGCCA4010.3377894586102617No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCAATCATCTAGG3830.3226268395205243No Hit
TGTAAAACGACGGCCAGTTGCTTCGGGGCATAGACAGCCATTCAACCAGC3820.32178447179331665No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGGGCCGGTCCGT3750.3158878977028632No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC3570.3007252786131258No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGCAGGATGGAG3510.29567107224987993No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCACCGAGGCAG3420.28808976270501124No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCATGTGAAGAGAG3160.26618820179761277No Hit
TGTAAAACGACGGCCAGTCACCAGCCATCTGGACCTGGTCATAGCTGTTT2960.24934084725346004No Hit
TGTAAAACGACGGCCAGTGGGAGGCCATGTTGAACTTATTCAGAATGTAC2910.24512900861742185No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTTGGACCCAGAG2800.23586296361813786No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT2710.2282816540732691No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCGCCTCGGCCTGC2680.22575455089164626No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTAGAGCCTGGCT2660.22406981543723098No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGACCGTGTCCGA2610.2198579768011928No Hit
TGTAAAACGACGGCCAGTAGGTCATCCTCCTGCCTCGGGTCAGTGTTCCT2440.205537725438663No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTTAGTATTTGAA2400.20216825452983248No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGGATGGTGTGGC2360.19879878362100192No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCAACCGT2350.19795641589379429No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCACAGCACTGCC2290.19290220953054849No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCAGCTCACT2190.1844785322584721No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG2180.18363616453126447No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCACATGCCTCCC2100.1768972227136034No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA1890.15920750044224305No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCAGGATGGAGGG1830.15415329407899725No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCACCCCGGCAGCGA1820.1533109263517896No Hit
TGTAAAACGACGGCCAGTTGCTTCGGGGCATAGACAGCGGAGGGGGGTCA1740.14657198453412854No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCGGCTCACT1620.1364635718076369No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTTGGGAAGGGA1450.12214332044510712No Hit
TGTAAAACGACGGCCAGTAGGTCATCCTCCTGCCTCGGTGGGGCCAGGTA1380.11624674635465365No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCAGCCGGGCTAGT1300.10950780453699259No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCACGTGTCGCCC1260.10613833362816204No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGACAGATGCCCC1250.1052959659009544No Hit
TGTAAAACGACGGCCAGTAGCACAGCTGCCCTTGGATCGACATGGAGAAA1220.1027688627193315No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)