FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003245746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52614
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA2800.5321777473676208No Hit
NNGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2620.4979663207511309No Hit
NNGGANNNNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA1760.3345117269167902No Hit
NNGGANNNNGNTATGNNNTGACAGCACAAGGAACACTGACCCGAGGCAGG1740.3307104572927358No Hit
NNGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1620.3079028395484092No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1560.29649903067624583No Hit
NNGGANNNNGNTATGNNNTGCACACACCTCCTATGTTAACAGGCACAAGT1430.27179077811989205No Hit
NAGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA1390.26418823887178317No Hit
NAGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1370.26038696924772875No Hit
NNGGANNNNGNTATGNNNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1310.24898316037556545No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1290.245181890751511No Hit
NNGGANNNNGNTATGNNNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC1260.23947998631542936No Hit
NNGGANNNNGNTATGNNNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC1220.2318774470673205No Hit
NNGGANNNNGNTATGNNNTGGGGATGAGGACAGCAGGGCAGTCACCATCT1170.2223742730071844No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1150.21857300338312996No Hit
NAGGANNNNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA1090.2071691945109667No Hit
NNGGANNNNGNTATGNNNGCCTCTCAGGGCCTCACAGTGGTCTGGGTCCT1090.2071691945109667No Hit
NNGGANNNNGNTATGANNTGACAGCACAAGGAACACTGACCCGAGGCAGG1090.2071691945109667No Hit
NNGGANNNNGNTATGNNNACCTTTACCCAAATAGCTACCACCAGTGCCTT1080.20526855969893945No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA1070.20336792488691224No Hit
NNGGANNNNGNTATGNNNCCCCAGGCCATTGTTGGAACAAAAAGCCTCAC1050.1995666552628578No Hit
NNGGANNNNGNTATGNNNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1030.19576538563880336No Hit
NNGGANNNNGNTATGNNNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC990.1881628463906945No Hit
NNGGANNNNGNTATGNNNCTTGAGCTCCTCGGACACGGCAGGAAACAGCT980.18626221157866726No Hit
NNGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA980.18626221157866726No Hit
NNGGANNNNGNTATGNNNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG970.18436157676664006No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC960.18246094195461282No Hit
NNGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT930.1767590375185312No Hit
NAGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC920.17485840270650396No Hit
NNGGANNNNGNTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC920.17485840270650396No Hit
NAGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC900.17105713308244952No Hit
NAGGANNNNGNTATGNNNTGACAGCACAAGGAACACTGACCCGAGGCAGG890.1691564982704223No Hit
NNGGANNNNGNTATGNNNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC880.1672558634583951No Hit
NNGGANNNNGNTATGNNNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT880.1672558634583951No Hit
NNGGANNNNGNTATGNNNCCACCCTCATGCCTAACCCCAGCACAGGGAGT880.1672558634583951No Hit
NAGGAAANAGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA870.1653552286463679No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC860.16345459383434066No Hit
NAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT860.16345459383434066No Hit
NNGGANNNNGNTATGNNNTGCTGGGAGAGGGGGATGAGTAGACAGGGCCA860.16345459383434066No Hit
NAGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT830.157752689398259No Hit
NNGGANNNNGNTATGANNTGCACACACCTCCTATGTTAACAGGCACAAGT830.157752689398259No Hit
NNGGANNNNGNTATGNNNCATGTGCCCCAGTAGAGAGCACAGGAAACAGC820.1558520545862318No Hit
NNGGANNNNGNTATGNNNACCGTTAAGCCACACCAAATGGTGAGCCCTGC810.1539514197742046No Hit
NNGGANNNNGNTATGANNTGCTGGGAGAGGGGGATGAGTAGACAGGGCCA810.1539514197742046No Hit
NNGGANNNNGNTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC800.15205078496217736No Hit
NAGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT800.15205078496217736No Hit
CAGGAAANAGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA780.14824951533812292No Hit
NNGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT770.1463488805260957No Hit
NAGGAANNNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA760.1444482457140685No Hit
NAGGANNNNGNTATGNNNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC740.14064697609001406No Hit
NNGGAAANNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA740.14064697609001406No Hit
NNGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC730.13874634127798685No Hit
NNGGAAANNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT730.13874634127798685No Hit
NAGGANNNNGNTATGNNNACCTTTACCCAAATAGCTACCACCAGTGCCTT710.1349450716539324No Hit
NNGGANNNNGNTATGANNCTTGAGCTCCTCGGACACGGCAGGAAACAGCT710.1349450716539324No Hit
NNGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC700.1330444368419052No Hit
NNGGANNNNGNTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG700.1330444368419052No Hit
NNGGANNNNGNTATGANNACCTTTACCCAAATAGCTACCACCAGTGCCTT690.131143802029878No Hit
NAGGANNNNGNTATGNNNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC690.131143802029878No Hit
NAGGANNNNGNTATGNNNACAGTGTCCCCATCAGGCTCCGGGACAGCATC690.131143802029878No Hit
NAGGAAANAGCTATGANNTGACAGCACAAGGAACACTGACCCGAGGCAGG690.131143802029878No Hit
NNGGANNNNGNTATGNNNCATGTGCCCCAGTAGAGAGCAGTGCCCCACTG690.131143802029878No Hit
NNGGANNNNGNTATGANNCCCCAGGCCATTGTTGGAACAAAAAGCCTCAC680.12924316721785076No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC680.12924316721785076No Hit
NNGGANNNNGNTATGNNNGCATCACTGAGGGAGGATCCTCCGGGCAGAGG680.12924316721785076No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA680.12924316721785076No Hit
NNGGANNNNGNTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT680.12924316721785076No Hit
NNGGANNNNGNTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC680.12924316721785076No Hit
NAGGANNNNGNTATGNNNGCCTCTCAGGGCCTCACAGTGGTCTGGGTCCT670.12734253240582355No Hit
NAGGANNNNGNTATGNNNCCCCAGGCCATTGTTGGAACAAAAAGCCTCAC670.12734253240582355No Hit
NNGGANNNNGNTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA670.12734253240582355No Hit
NAGGAAANAGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT660.12544189759379631No Hit
NNGGANNNNGNTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT660.12544189759379631No Hit
NAGGAAANAGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA660.12544189759379631No Hit
NAGGANNNNGNTATGNNNTGCTGGGAGAGGGGGATGAGTAGACAGGGCCA650.1235412627817691No Hit
NAGGAANNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT650.1235412627817691No Hit
NAGGANNNNGNTATGNNNTGCACACACCTCCTATGTTAACAGGCACAAGT640.1216406279697419No Hit
NNGGANNNNGNTATGANNCATGTGCCCCAGTAGAGAGCACAGGAAACAGC640.1216406279697419No Hit
NNGGANNNNGNTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA640.1216406279697419No Hit
NNGGANNNNGNTATGNNNACAGGGGCCTCTTAAAACTGCAAGCCTGACTG630.11973999315771468No Hit
NNGGANNNNGNTATGNNNCTCAGCTCTGCAGCCTCCGCACAGACCCCACA620.11783935834568746No Hit
NNGGANANAGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA610.11593872353366025No Hit
CAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT610.11593872353366025No Hit
NNGGANNNNGNTATGNNNATCGCACCACTGCACTCCAGCGCTCCATACAC600.11403808872163303No Hit
CAGGAAANAGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT590.1121374539096058No Hit
NNGGANNNNGNTATGANNCCACCCTCATGCCTAACCCCAGCACAGGGAGT590.1121374539096058No Hit
NNGGANNNNGNTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT590.1121374539096058No Hit
NAGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC580.11023681909757858No Hit
NNGGAAANNGNTATGANNTGACAGCACAAGGAACACTGACCCGAGGCAGG580.11023681909757858No Hit
NAGGANNNNGNTATGNNNTGGGGATGAGGACAGCAGGGCAGTCACCATCT570.10833618428555138No Hit
NNGGANNNNGNTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT570.10833618428555138No Hit
NNGGANNNNGNTATGNNNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA560.10643554947352415No Hit
NAGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA560.10643554947352415No Hit
NNGGANNNNGNTATGANNGCCTCTCAGGGCCTCACAGTGGTCTGGGTCCT550.10453491466149695No Hit
NNGGANNNNGNTATGNNNGCCTCTCAGGGCCTCACAGTGGACACACCACT550.10453491466149695No Hit
NNGGANNNNGNTATGNNNGAGGCGCAGGTCACAGTGAGACCAATACAGAG550.10453491466149695No Hit
NNGGANNNNGNTATGNNNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT550.10453491466149695No Hit
NNGGANNNNGNNATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA550.10453491466149695No Hit
NNGGANNNNGNTATGNNNAGCGTCTCATTCCATAAAGGCCACCATGAAAC540.10263427984946973No Hit
NAGGANNNNGNTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA540.10263427984946973No Hit
NNGGANNNNGNTATGNNNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT540.10263427984946973No Hit
NAGGANNNNGNTATGANNTGACAGCACAAGGAACACTGACCCGAGGCAGG540.10263427984946973No Hit
NNGGANNNNGNTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC530.1007336450374425No Hit
NNGGANNNNGNTATGNNNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT530.1007336450374425No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)