FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88853
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNACTCCCTTGAGCCTTTGGGGCTGTTGTCACTA9171.032041686831058No Hit
CAGGAAANAGCTATGACCACTCCCTTGAGCCTTTGGGGCTGTTGTCACTA8800.990399873949107No Hit
CAGGAAANAGCTATGANNGCAGCAAGAGACCCAGACCCACCCGACTGGCC8550.9622635138937345No Hit
CAGGAAANAGCTATGACCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT8340.9386289714472218No Hit
CAGGAAANAGCTATGACCGCAGCAAGAGACCCAGACCCACCCGACTGGCC7660.8620980720966089No Hit
CAGGAAANAGCTATGANNGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT7460.8395889840523111No Hit
CAGGAAANAGCTATGACCGTTTTCACTGGCTCCCGGGGCTGTTCACTGGC7450.8384635296500962No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCAGAGGGAAGGTGACT7390.8317108032368068No Hit
CAGGAAANAGCTATGACCACCAAGACCCCAGCAGCAGAGGGAAGGTGACT7120.8013235343770048No Hit
CAGGAAACAGCTATGACCACTCCCTTGAGCCTTTGGGGCTGTTGTCACTA6470.7281689982330366No Hit
CAGGAAANAGCTATGANNGTTTTCACTGGCTCCCGGGGCTGTTCACTGGC6420.7225417262219621No Hit
CAGGAAANAGCTATGANNGCTTCTATATGAGCGCATCTCACCTGAATTTG6290.7079108189931684No Hit
CAGGAAANAGCTATGACCGTTTTCACTTGCTTCCGGGGCTGTTCACTGGC6270.7056599101887386No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT6200.6977817293732345No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGCTGTTCACTGGC5890.6628926429045727No Hit
CAGGAAACAGCTATGACCGCAGCAAGAGACCCAGACCCACCCGACTGGCC5800.6527635532846386No Hit
CAGGAAANAGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG5770.6493871900779941No Hit
CAGGAAANAGCTATGANNGTTTTCACTTGCTTCCGGGGCTGTTCACTGGC5620.6325053740447706No Hit
CAGGAAANAGCTATGANNGCTTCTATATGAGCGCATCTCACCAGAGCACC5530.6223762844248365No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCAGAGGGAAGGTGACT5380.605494468391613No Hit
CAGGAAANAGCTATGACCGCTTCTATATGAGCGCATCTCACCTGAATTTG5160.5807344715428854No Hit
CAGGAAANAGCTATGACCTGCTCTGGGCTCTGGATACTGGCCGTCGTTTT5040.5672290187163067No Hit
CAGGAAANAGCTATGACCCTGTGAGGCACCAGAGCACCGACTGGCCGTCG4800.5402181130631493No Hit
CAGGAAANAGCTATGANNTGCTCTGGGCTCTGGATACTGGCCGTCGTTTT4720.5312144778454301No Hit
CAGGAAACAGCTATGACCGTTTTCACTTGCTTCCGGGGCTGTTCACTGGC4640.522210842627711No Hit
CAGGAAANAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG4580.5154581162144216No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGACTGGCCGTCG4510.5075799353989173No Hit
CAGGAAANAGCTATGANNGCTTCTATATGAGCGCATCTCACCAACCTTGG4250.4783181209413301No Hit
CAGGAAANAGCTATGACCGCTTCTATATGAGCGCATCTCACCAGAGCACC4220.4749417577346854No Hit
CAGGAAANAGCTATGANCACTCCCTTGAGCCTTTGGGGCTGTTGTCACTA4170.4693144857236109No Hit
CAGGAAANAGCTATGANCGCAGCAAGAGACCCAGACCCACCCGACTGGCC4150.4670635769191811No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGACTGGCCGTCG3930.44230358007045345No Hit
CAGGAAACAGCTATGACCGCTTCTATATGAGCGCATCTCACCTGAATTTG3800.42767267284165983No Hit
CAGGAAANAGCTATGANNAACCAACACTGCTGCTAATTGTGTTTGTGTCC3560.4006617671885024No Hit
CAGGAAACAGCTATGACCTGCTCTGGGCTCTGGATACTGGCCGTCGTTTT3520.3961599495796428No Hit
CAGGAAANAGCTATGACCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG3470.3905326775685683No Hit
CAGGAAANAGCTATGANNACTCCCAACTCTTGGCTGTGCCACTGGCCGTC3410.38377995115527896No Hit
CAGGAAANAGCTATGANCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT3410.38377995115527896No Hit
CAGGAAANAGCTATGANNACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG3280.36914904392648534No Hit
CAGGAAANAGCTATGANCACCAAGACCCCAGCAGCAGAGGGAAGGTGACT3280.36914904392648534No Hit
CAGGAAANAGCTATGACCGCTTCTATATGAGCGCATCTCACCAACCTTGG3260.36689813512205555No Hit
CAGGAAANAGCTATGACCACTCCCAACTCTTGGCTGTGCCACTGGCCGTC3240.36464722631762575No Hit
CAGGAAANAGCTATGANNGCTTCTATATGAGCCCATCTCACCAGAGCACC3190.35901995430655126No Hit
CAGGAAANAGCTATGANCGTTTTCACTGGCTCCCGGGGCTGTTCACTGGC3120.35114177349104697No Hit
CAGGAAANAGCTATGACCAACCAACACTGCTGCTAATTGTGTTTGTGTCC2970.3342599574578236No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG2920.3286326854467491No Hit
CAGGAAACAGCTATGACCGCTTCTATATGAGCGCATCTCACCAGAGCACC2870.3230054134356747No Hit
CAGGAAANAGCTATGANNGCTTCTATATGAGCCCATCTCACCAACCTTGG2820.3173781414246002No Hit
CAGGAAANAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2790.3140017782179555No Hit
CAGGAAANAGCTATGANNTGCCAAAATTAAGGATGCACACCTGCCTCACT2710.3049981430002363No Hit
CAGGAAANAGCTATGANCGCTTCTATATGAGCGCATCTCACCTGAATTTG2680.3016217797935916No Hit
CAGGAAACAGCTATGACCACTCCCAACTCTTGGCTGTGCCACTGGCCGTC2610.2937435989780874No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2570.2892417813692278No Hit
CAGGAAANAGCTATGANCGTTTTCACTTGCTTCCGGGGCTGTTCACTGGC2570.2892417813692278No Hit
CAGGAAACAGCTATGACCGCTTCTATATGAGCGCATCTCACCAACCTTGG2550.28699087256479805No Hit
CAGGAAANAGCTATGACCGCTTCTATATGAGCCCATCTCACCAGAGCACC2520.28361450935815335No Hit
CAGGAAANAGCTATGANNTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC2520.28361450935815335No Hit
CAGGAAANAGCTATGACCTGCCAAAATTAAGGATGCACACCTGCCTCACT2490.28023814615150866No Hit
CAGGAAANAGCTATGACCGCTTCTATATGAGCCCATCTCACCAACCTTGG2490.28023814615150866No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG2450.2757363285426491No Hit
CAGGAAANAGCTATGANCCTGTGAGGCACCAGAGCACCGACTGGCCGTCG2400.27010905653157463No Hit
CAGGAAANAGCTATGANCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG2380.26785814772714484No Hit
CAGGAAANAGCTATGACCGCAGCCACCCTGAACACACTGGCCGTCGTTTT2300.2588545125094257No Hit
CAGGAAANAGCTATGANNGCAGCCACCCTGAACACACTGGCCGTCGTTTT2240.2521017860961363No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2230.2509763316939214No Hit
CAGGAAANAGCTATGANCGCTTCTATATGAGCGCATCTCACCAGAGCACC2190.24647451408506185No Hit
CAGGAAANAGCTATGANCTGCTCTGGGCTCTGGATACTGGCCGTCGTTTT2140.24084724207398736No Hit
CAGGAAACAGCTATGACCAACCAACACTGCTGCTAATTGTGTTTGTGTCC2100.2363454244651278No Hit
CAGGAAANAGCTATGACCAGGAAGAAGCCAAGGCCCAGACTGGCCGTCGT1950.21946360843190438No Hit
CAGGAAACAGCTATGACCGCTTCTATATGAGCCCATCTCACCAACCTTGG1790.2014563379964661No Hit
CAGGAAANAGCTATGACCCTTCCATGCTGGGCTCTGGACTGGCCGTCGTT1780.2003308835942512No Hit
CAGGAAANAGCTATGANCGCTTCTATATGAGCGCATCTCACCAACCTTGG1770.1992054291920363No Hit
CAGGAAACAGCTATGACCGCAGCCACCCTGAACACACTGGCCGTCGTTTT1700.19132724837653203No Hit
CAGGAAANAGCTATGACCTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC1690.19020179397431713No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1680.18907633957210224No Hit
CAGGAAACAGCTATGACCGCTTCTATATGAGCCCATCTCACCAGAGCACC1620.18232361315881288No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGCCTCACT1610.18119815875659798No Hit
CAGGAAANAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1610.18119815875659798No Hit
CAGGAAANAGCTATGANNGCAGCAAGAGACCAATACCCACCCGACTGGCC1580.17782179554995328No Hit
CAGGAAANAGCTATGACCGCAGCAAGAGACCAATACCCACCCGACTGGCC1560.17557088674552349No Hit
CAGGAAACAGCTATGACCAGGAAGAAGCCAAGGCCCAGACTGGCCGTCGT1550.17444543234330862No Hit
CAGGAAANAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1500.16881816033223415No Hit
CAGGAAANAGCTATGANCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG1490.16769270593001925No Hit
CAGGAAANAGCTATGANNAGGAAGAAGCCAAGGCCCAGACTGGCCGTCGT1480.16656725152780436No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1460.16431634272337456No Hit
CAGGAAANAGCTATGANCAACCAACACTGCTGCTAATTGTGTTTGTGTCC1430.1609399795167299No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1420.159814525114515No Hit
CAGGAAACAGCTATGACCCTTCCATGCTGGGCTCTGGACTGGCCGTCGTT1370.1541872531034405No Hit
CAGGAAANAGCTATGANNCTTCCATGCTGGGCTCTGGACTGGCCGTCGTT1360.1530617987012256No Hit
CAGGAAANAGCTATGANCGCTTCTATATGAGCCCATCTCACCAGAGCACC1320.14855998109236604No Hit
CAGGAAANAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1300.14630907228793624No Hit
CAGGAAANAGCTATGANCGCTTCTATATGAGCCCATCTCACCAACCTTGG1290.14518361788572134No Hit
CAGGAAANAGCTATGACCACAGGCAGGAGGGAGTACTACTGGCCGTCGTT1280.14405816348350647No Hit
CAGGAAANAGCTATGANCACTCCCAACTCTTGGCTGTGCCACTGGCCGTC1250.14068180027686178No Hit
CAGGAAANAGCTATGACCTGTTGTCTTTCAGGGGCCGCAGCAGTAATGGA1220.1373054370702171No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC1190.13392907386357242No Hit
CAGGAAANAGCTATGANNTGTTGTCTTTCAGGGGCCGCAGCAGTAATGGA1190.13392907386357242No Hit
CAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1190.13392907386357242No Hit
CAGGAAANAGCTATGANNACAGGCAGGAGGGAGTACTACTGGCCGTCGTT1130.12717634745028306No Hit
CAGGAAANAGCTATGACCGCAGCTCCCCACAACTTCAACTGGCCGTCGTT1130.12717634745028306No Hit
NAGGAAANAGCTATGANNACTCCCTTGAGCCTTTGGGGCTGTTGTCACTA1080.12154907543920858No Hit
CAGGAAACAGCTATGACCGCAGCAAGAGACCAATACCCACCCGACTGGCC1070.12042362103699368No Hit
CAGGAAANAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1050.1181727122325639No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCATTGGGAAGGTGACT1040.117047257830349No Hit
CAGGAAANAGCTATGANNTTCCCTGTGCCCATCCAGCCCACTGGCCGTCG1040.117047257830349No Hit
CAGGAAANAGCTATGANCGCAGCCACCCTGAACACACTGGCCGTCGTTTT1040.117047257830349No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1030.1159218034281341No Hit
CAGGAAANAGCTATGANNGGGAATTGGAGGCAGGCTTGCACTGGCCGTCG1020.1147963490259192No Hit
CAGGAAANAGCTATGANNGCAGCTCCCCACAACTTCAACTGGCCGTCGTT1010.11367089462370433No Hit
CAGGAAANAGCTATGACCGGGAATTGGAGGCAGGCTTGCACTGGCCGTCG1010.11367089462370433No Hit
CAGGAAANAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1000.11254544022148943No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG980.11029453141705964No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG970.10916907701484474No Hit
CAGGAAANAGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT960.10804362261262984No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCAGCAGTAATGGA950.10691816821041496No Hit
CAGGAAANAGCTATGACCTTCCCTGTGCCCATCCAGCCCACTGGCCGTCG940.10579271380820006No Hit
CAGGAAANAGCTATGANNGGGAGGTGGAGGTTGCACTGGCCGTCGTTTTA940.10579271380820006No Hit
CAGGAAACAGCTATGACCGGGAATTGGAGGCAGGCTTGCACTGGCCGTCG940.10579271380820006No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGTAGAGTGTATACA940.10579271380820006No Hit
NAGGAAANAGCTATGANNGCAGCAAGAGACCCAGACCCACCCGACTGGCC920.10354180500377026No Hit
CAGGAAANAGCTATGANNCTCAGCCCTGCAGCAGTTACCACACTGGCCGT920.10354180500377026No Hit
CAGGAAANAGCTATGANNTCCTCTTCATTTTCACGGTGCCATTCTGATTC900.1012908961993405No Hit
CAGGAAACAGCTATGANCGCAGCAAGAGACCCAGACCCACCCGACTGGCC900.1012908961993405No Hit
NAGGAAANAGNTATGANNACTCCCTTGAGCCTTTGGGGCTGTTGTCACTA900.1012908961993405No Hit
NAGGAAANAGNTATGANNGCAGCAAGAGACCCAGACCCACCCGACTGGCC890.1001654417971256No Hit
CAGGAAANAGCTATGACCCTCAGCCCTGCAGCAGTTACCACACTGGCCGT890.1001654417971256No Hit
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC890.1001654417971256No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)