FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133627
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAANGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA34032.54664102314652No Hit
TGTAAAANGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG32482.4306465010813683No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA31232.337102531673988No Hit
TGTAAAANGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA29632.217366250832541No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG28022.0968816182358356No Hit
TGTAAAANGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT26141.9561914882471356No Hit
TGTAAAANGACGGCCAGTGGAGCGACTTGACAATGCCCCTCACACCCCTT24561.8379519109162072No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA24191.8102628959716225No Hit
TGTAAAACGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT23491.7578782731034897No Hit
TGTAAAACGACGGCCAGTGGAGCGACTTGACAATGCCCCTCACACCCCTT22101.6538573791224827No Hit
TGTAAAANGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT19281.4428221841394329No Hit
TGTAAAANGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC17101.279681501492962No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT16891.2639661146325218No Hit
TGTAAAANGACGGCCAGTTGACACAGCTGCTTCTGAGTACAAGTATGTAG15281.1434814820358161No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC14921.1165408188464907No Hit
TGTAAAACGACGGCCAGTTGACACAGCTGCTTCTGAGTACAAGTATGTAG13641.0207517941733333No Hit
TGTAAAANGACGGCCAGTACACTCCCTCCACCTCCCATCCCAGAAAAAGT13531.0125199248654837No Hit
TGTAAAANGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA13000.9728572818367545No Hit
TGTAAAACGACGGCCAGTACACTCCCTCCACCTCCCATCCCAGAAAAAGT12470.9331946388080252No Hit
TGTAAAANGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC11310.8463858351979764No Hit
TGTAAAANGACGGCCAGTTCACAGTGCTGTAGGGGCCTACACGTTCCAGG11140.8336638553585728No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA11090.8299220965822776No Hit
TGTAAAANGACGGCCAGTAACAAATTCAGGTGAGATGCGCTCATATAGAA10780.8067231921692471No Hit
TGTAAAANGACGGCCAGTACACATTCTTGTAAAGCATGGGATTGCTGAAT10590.7925045088193255No Hit
TGTAAAANGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC9980.7468550517485238No Hit
TGTAAAANGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT9790.732636368398602No Hit
TGTAAAANGACGGCCAGTGTTCTACCGTCAGTCCTGGCATTATTTCTCTC9730.7281462578670479No Hit
TGTAAAACGACGGCCAGTAACAAATTCAGGTGAGATGCGCTCATATAGAA9660.7229077955802345No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC9640.7214110920697164No Hit
TGTAAAACGACGGCCAGTTCACAGTGCTGTAGGGGCCTACACGTTCCAGG9260.6929737253698729No Hit
TGTAAAACGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC9140.6839935043067643No Hit
TGTAAAACGACGGCCAGTACACATTCTTGTAAAGCATGGGATTGCTGAAT8960.6705231727121016No Hit
TGTAAAANGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT8870.6637880069147702No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT8810.6592978963832159No Hit
TGTAAAACGACGGCCAGTGTTCTACCGTCAGTCCTGGCATTATTTCTCTC8570.641337454256999No Hit
TGTAAAANGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG8430.6308605296833724No Hit
TGTAAAANGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGACCAGACG8300.6211319568650048No Hit
TGTAAAANGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC8300.6211319568650048No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGACCAGACG8220.6151451428229325No Hit
TGTAAAACGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT7960.5956879971861974No Hit
TGTAAAANGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT7920.5926945901651612No Hit
TGTAAAACGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG7860.588204479633607No Hit
TGTAAAANGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC7820.5852110726125708No Hit
TGTAAAANGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG7730.5784759068152395No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGGAGGAGTGGTCATAG7660.5732374445284261No Hit
TGTAAAANGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCATTTATT7560.5657539269758358No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGCGGTCATA7380.552283595381173No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC7270.5440517260733235No Hit
TGTAAAANGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGCGGTCATA7270.5440517260733235No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT7200.5388132637865102No Hit
TGTAAAANGACGGCCAGTAACAAATTCAGGTGAGATGCGCTCACATAGAA6990.5230978769260703No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG6900.516362711128739No Hit
TGTAAAACGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCATTTATT6900.516362711128739No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC6880.5148660076182209No Hit
TGTAAAANGACGGCCAGTGGGGAGAGGAAGGCCAGGAGGAGTGGTCATAG6760.5058857865551124No Hit
TGTAAAACGACGGCCAGTAACAAATTCAGGTGAGATGCGCTCACATAGAA6700.5013956760235581No Hit
TGTAAAANGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA6270.4692165505474193No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT6110.4572429224632747No Hit
TGTAAAANGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC6050.4527528119317204No Hit
TGTAAAANGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT6000.44901105315542517No Hit
TGTAAAANGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT5800.4340440180502443No Hit
TGTAAAACGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA5690.42581214874239487No Hit
TGTAAAANGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA5450.40785170661617787No Hit
TGTAAAANGACGGCCAGTTCCATTACTCTACACAAGCAGGGTACACTTAG5440.40710335486091886No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT5430.4063550031056598No Hit
TGTAAAANGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG5080.3801626916715933No Hit
TGTAAAANGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG5050.3779176364058162No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC4930.3689374153427077No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTACACTTAG4910.36744071183218957No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT4720.3532220284822678No Hit
TGTAAAANGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT4500.33675828986656886No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG4410.3300231240692375No Hit
TGTAAAANGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT4270.3195461994956109No Hit
TGTAAAANGACGGCCAGTCCTGCCGCCTGGTCTCACCTCGCTATGGTTCG4240.3173011442298338No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG4180.3128110336982795No Hit
TGTAAAANGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA4160.31131433018776145No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA4110.30757257141146627No Hit
TGTAAAANGACGGCCAGTTCCCACCACCATACCCCAACTTAGTATTTGAA4030.3015857573693939No Hit
TGTAAAANGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG3930.2941022398168035No Hit
TGTAAAANGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC3700.27689014944584556No Hit
TGTAAAANGACGGCCAGTCCTGCCGCCTGGTCTCACCTCGCCATGGTTCG3610.27015498364851415No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTTAGTATTTGAA3590.26865828013799603No Hit
TGTAAAANGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG3530.26416816960644185No Hit
TGTAAAACGACGGCCAGTCCTGCCGCCTGGTCTCACCTCGCTATGGTTCG3480.2604264108301466No Hit
TGTAAAACGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG3410.2551879485433333No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA3250.24321432045918862No Hit
TGTAAAANGACGGCCAGTCGCACAGTCTGTTCTGCAGCAACAACGGGATT3220.2409692651934115No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT3140.2349824511513392No Hit
TGTAAAACGACGGCCAGTCCTGCCGCCTGGTCTCACCTCGCCATGGTTCG3050.2282472853540078No Hit
TGTAAAANGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG3040.22749893359874876No Hit
TGTAAAANGACGGCCAGTGTGGTAACTGCTGCAGGGCTTAATACATTAGT2930.2192670642908993No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC2920.21851871253564026No Hit
TGTAAAANGACGGCCAGTGCCCAAGGTTGGTGAGATGCGCTCATATAGAA2910.2177703607803812No Hit
TGTAAAANGACGGCCAGTCTTTCCCAACCCTCTGCCCGCCGGTGCCCATG2870.21477695375934505No Hit
TGTAAAACGACGGCCAGTCGCACAGTCTGTTCTGCAGCAACAACGGGATT2870.21477695375934505No Hit
TGTAAAACGACGGCCAGTGCCCAAGGTTGGTGAGATGCGCTCATATAGAA2820.21103519498304985No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG2820.21103519498304985No Hit
TGTAAAANGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC2770.20729343620675464No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGCCGGTGCCCATG2630.19681651163312805No Hit
TGTAAAACGACGGCCAGTGCCATCGGCTCCCAAGTTTTATGTCCTAGAGC2530.1893329940805376No Hit
TGTAAAANGACGGCCAGTAGCCCCTCGTCACCTACTCCCCGAAGTTACCA2450.18334618003846528No Hit
TGTAAAANGACGGCCAGTGCCATCGGCTCCCAAGTTTTATGTCCTAGAGC2340.1751143107306158No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTTAATACATTAGT2270.16987584844380252No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG2240.1676307931780254No Hit
TGTAAAACGACGGCCAGTAGCCCCTCGTCACCTACTCCCCGAAGTTACCA2160.16164397913595305No Hit
TGTAAAANGACGGCCAGTTCCATCCACTGCTGAGAAATCTCCTCAAACCC2120.1586505721149169No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC2100.1571538686043988No Hit
TGTAAAANGACGGCCAGTAGTACTCCCTCCTGCCTGTGGTCATAGCTGTT2080.15565716509388072No Hit
TGTAAAANGACGGCCAGTGGTCCCACTCTGGCTTTCCCACCTGATAATAA1950.1459285922755132No Hit
TGTAAAANGACGGCCAGTCCTTGAAGGCTGGGCTTCTGTATGAAGGTTGG1830.13694837121240466No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGTCATAGCTGTT1780.13320661243610948No Hit
TGTAAAACGACGGCCAGTCCTTGAAGGCTGGGCTTCTGTATGAAGGTTGG1700.12721979839403713No Hit
TGTAAAANGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG1690.1264714466387781No Hit
TGTAAAACGACGGCCAGTGGTCCCACTCTGGCTTTCCCACCTGATAATAA1670.12497474312826001No Hit
TGTAAAACGACGGCCAGTTCCATCCACTGCTGAGAAATCTCCTCAAACCC1610.12048463259670575No Hit
TGTAAAANGACGGCCAGTAGATCATCCAGCGCACACGGCTGCTGGACAGT1490.11150441153359725No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG1470.11000770802307917No Hit
TGTAAAANGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG1470.11000770802307917No Hit
TGTAAAACGACGGCCAGTAGATCATCCAGCGCACACGGCTGCTGGACAGT1470.11000770802307917No Hit
TGTAAAANGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA1450.10851100451256107No Hit
TGTAAAANGACGGCCAGTCCTGGCCCACAAACTGTGCTCAGTAAATGGTA1440.10776265275730204No Hit
TGTAAAACGACGGCCAGTGCCCAAGGTTGGTGAGATGGGCTCATATAGAA1430.107014301002043No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA1410.10551759749152492No Hit
TGTAAAACGACGGCCAGTCCTGGCCCACAAACTGTGCTCAGTAAATGGTA1400.10476924573626588No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG1370.10252419047048875No Hit
TGTAAAANGACGGCCAGTGCCCAAGGTTGGTGAGATGGGCTCATATAGAA1370.10252419047048875No Hit
TGTAAAANGACGGCCAGTACACTCCCCTCACCCCACCCTAGGGTCATAGC1370.10252419047048875No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)