FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133627
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG21471.6067112185411632No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA21031.573783741309765No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT16931.266959521653558No Hit
CAGGAAANAGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA15591.1666803864488464No Hit
CAGGAAANAGCTATGANNGCTTCTATATGAGCGCATCTCACCTGAAGTTG13691.0244935529496284No Hit
CAGGAAANAGCTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC12120.9070023273739588No Hit
CAGGAAANAGCTATGANNGCAAAACACCTTAACACTCGGCTTCTATTTGC12030.9002671615766276No Hit
CAGGAAANAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA11490.8598561667926392No Hit
CAGGAAANAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG11010.8239352825402052No Hit
CAGGAAANAGCTATGANNAACCAACACTGCTGCTAATTGTGTTTGTGTCC9060.6780066902646921No Hit
CAGGAAANAGCTATGANNCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA8920.6675297656910655No Hit
CAGGAAANAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT8620.6450792130332942No Hit
CAGGAAANAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA8530.6383440472359627No Hit
CAGGAAANAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG7700.5762308515494623No Hit
CAGGAAANAGCTATGACCGCTTCTATATGAGCGCATCTCACCTGAAGTTG7220.5403099672970283No Hit
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG7190.5380649120312512No Hit
CAGGAAANAGCTATGANCAGGCACCAACAGTACAAAGCAGATACAAAGCA6970.5216011734155522No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA6910.517111062883998No Hit
CAGGAAANAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC6900.516362711128739No Hit
CAGGAAANAGCTATGANNTCAAGCAAGTTCAGGATGGTATAGAAATAGAG6770.5066341383103714No Hit
CAGGAAANAGCTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT6650.49765391724726293No Hit
CAGGAAANAGCTATGANNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC6390.4781967716105278No Hit
CAGGAAANAGCTATGANNAGAGACCAGATGCATAAGCACAGCAGCATTCC6340.4744550128342326No Hit
CAGGAAANAGCTATGANNCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG6180.46248138475008793No Hit
CAGGAAANAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC6160.4609846812395698No Hit
CAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT6110.4572429224632747No Hit
CAGGAAANAGCTATGANNTCCTCTTCATTTTCACGGTGCCATTCTGATTC5460.40860005837143687No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG5440.40710335486091886No Hit
CAGGAAANAGCTATGANCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT5360.40111654081884646No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA5310.3973747820425513No Hit
CAGGAAANAGCTATGANNCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC5250.392884671510997No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT4990.3734275258742619No Hit
CAGGAAANAGCTATGANCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA4950.3704341188532258No Hit
CAGGAAANAGCTATGANCGCTTCTATATGAGCGCATCTCACCTGAAGTTG4820.3607055460348582No Hit
CAGGAAANAGCTATGANNGCAGCAAGAGACCCAGACCCACCCGACTGGCC4800.35920884252434016No Hit
CAGGAAANAGCTATGANNCGCTTCTGGGGACTTTGCTCTTGGGCTCTTTT4800.35920884252434016No Hit
CAGGAAANAGCTATGANNGGCTGGCACAAAGAACAGCACTGACTGTTTAT4740.35471873199278586No Hit
CAGGAAANAGCTATGACCAACCAACACTGCTGCTAATTGTGTTTGTGTCC4730.35397038023752686No Hit
CAGGAAANAGCTATGANNCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG4670.34948026970597257No Hit
CAGGAAANAGCTATGANNGTCAACAGCATACCTGTACACCTGAAATAACT4650.3479835661954545No Hit
CAGGAAANAGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT4640.34723521444019545No Hit
CAGGAAANAGCTATGANNACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG4630.34648686268493645No Hit
CAGGAAACAGCTATGACCGCTTCTATATGAGCGCATCTCACCTGAAGTTG4610.3449901591744184No Hit
CAGGAAANAGCTATGACCCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA4470.33451323460079174No Hit
CAGGAAACAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC4270.3195461994956109No Hit
CAGGAAANAGCTATGANNTCCTCTTCATTTTCACGGTGCCATTCCGATTC4220.31580444071931574No Hit
CAGGAAANAGCTATGANCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC4140.3098176266772434No Hit
CAGGAAANAGCTATGANCGCAAAACACCTTAACACTCGGCTTCTATTTGC3950.2955989433273216No Hit
CAGGAAANAGCTATGANNGCAGCAAGAGACCAATACCGCATTGTAGTCAA3860.2888637775299902No Hit
CAGGAAANAGCTATGANNTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC3840.2873670740194721No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC3830.28661872226421303No Hit
CAGGAAANAGCTATGANNGCTTCTATATGAGCGCATCTCACCTGAATTTG3820.28587037050895403No Hit
CAGGAAANAGCTATGACCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG3750.28063190822214074No Hit
CAGGAAANAGCTATGANNCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT3640.27240003891429126No Hit
CAGGAAANAGCTATGACCGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC3600.2694066318932551No Hit
CAGGAAANAGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT3600.2694066318932551No Hit
CAGGAAANAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT3560.26641322487221897No Hit
CAGGAAANAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT3480.2604264108301466No Hit
CAGGAAANAGCTATGACCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG3400.25443959678807426No Hit
CAGGAAANAGCTATGACCAGAGACCAGATGCATAAGCACAGCAGCATTCC3340.24994948625652003No Hit
CAGGAAACAGCTATGACCCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA3330.24920113450126097No Hit
CAGGAAANAGCTATGANCCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA3280.24545937572496576No Hit
CAGGAAANAGCTATGANNACAGGGGCCTCTTAAAACTGCATGTGTTCAGC3200.2394725616828934No Hit
CAGGAAACAGCTATGACCAACCAACACTGCTGCTAATTGTGTTTGTGTCC3130.23423409939608014No Hit
CAGGAAANAGCTATGACCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG3100.231989044130303No Hit
CAGGAAANAGCTATGACCGCAGCAAGAGACCCAGACCCACCCGACTGGCC3040.22749893359874876No Hit
CAGGAAANAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG3020.2260022300882307No Hit
CAGGAAANAGCTATGACCGTCAACAGCATACCTGTACACCTGAAATAACT2980.2230088230671945No Hit
CAGGAAANAGCTATGANNAAGGACAAGGTGAGGCAGCAAGTGCTGATGTA2980.2230088230671945No Hit
CAGGAAANAGCTATGANNCCTGGACGCAATCACCCAGGCCAGCTAAGTGA2900.21702200902512214No Hit
CAGGAAANAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT2840.2125318984935679No Hit
CAGGAAANAGCTATGANNTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC2800.20953849147253176No Hit
CAGGAAANAGCTATGANNGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG2730.20430002918571843No Hit
CAGGAAANAGCTATGANNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT2730.20430002918571843No Hit
CAGGAAANAGCTATGANNCCTTGGGCAAGTTAACAAACCTCTCTGAGCCT2650.19831321514364614No Hit
CAGGAAANAGCTATGANCAACCAACACTGCTGCTAATTGTGTTTGTGTCC2650.19831321514364614No Hit
CAGGAAANAGCTATGACCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC2640.19756486338838708No Hit
CAGGAAANAGCTATGACCTCCTCTTCATTTTCACGGTGCCATTCCGATTC2570.19232640110157379No Hit
CAGGAAANAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGGCTCTTTT2540.19008134583579664No Hit
CAGGAAANAGCTATGACCTCCTCTTCATTTTCACGGTGCCATTCTGATTC2500.1870879388147605No Hit
CAGGAAANAGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2490.18633958705950143No Hit
CAGGAAANAGCTATGACCCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG2480.1855912353042424No Hit
CAGGAAACAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT2470.18484288354898337No Hit
CAGGAAANAGCTATGANCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT2430.1818494765279472No Hit
CAGGAAANAGCTATGANNACTCCTCCTGGCCTTCCTCTTTCAGAGATGGT2420.18110112477268814No Hit
CAGGAAACAGCTATGACCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG2390.17885606950691105No Hit
CAGGAAANAGCTATGANNAGAAAGAAGAAGCAGAGCAAAAACAATAGTCT2360.1766110142411339No Hit
CAGGAAANAGCTATGANNTGCCAAAATTAAGGATGCACACCTGTGACACA2340.1751143107306158No Hit
CAGGAAANAGCTATGANNAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT2290.1713725519543206No Hit
CAGGAAACAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT2190.1638890344017302No Hit
CAGGAAANAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT2170.1623923308912121No Hit
CAGGAAANAGCTATGANCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG2170.1623923308912121No Hit
CAGGAAACAGCTATGACCAGAGACCAGATGCATAAGCACAGCAGCATTCC2170.1623923308912121No Hit
CAGGAAANAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2130.15939892387017593No Hit
CAGGAAANAGCTATGANNCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC2110.15790222035965787No Hit
CAGGAAANAGCTATGANNGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT2070.1549088133386217No Hit
CAGGAAACAGCTATGACCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG2060.15416046158336266No Hit
CAGGAAANAGCTATGANCCAGGGCTCCAAGAATGTTCCACACATCTTTCT2050.1534121098281036No Hit
CAGGAAANAGCTATGACCGCAGCAAGAGACCAATACCGCATTGTAGTCAA2050.1534121098281036No Hit
CAGGAAANAGCTATGANCGCAGCAAGAGACCCAGACCCACCCGACTGGCC2020.15116705456232649No Hit
CAGGAAACAGCTATGACCGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC2020.15116705456232649No Hit
CAGGAAANAGCTATGACCGGCTGGCACAAAGAACAGCACTGACTGTTTAT2020.15116705456232649No Hit
CAGGAAANAGCTATGANNGCTTCTATATGAGCGCATCTCACCAACCTTGG2010.15041870280706743No Hit
CAGGAAANAGCTATGANCGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC2000.1496703510518084No Hit
CAGGAAANAGCTATGANNTGCTGCCGAGAGAGGACAGTGATCCCCCAAAA1980.1481736475412903No Hit
CAGGAAANAGCTATGANCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG1960.14667694403077222No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG1950.1459285922755132No Hit
CAGGAAANAGCTATGANNACACATACTCCCTTGCTCCAAACTTATAACAA1930.1444318887649951No Hit
CAGGAAANAGCTATGANCAGAGACCAGATGCATAAGCACAGCAGCATTCC1920.14368353700973605No Hit
CAGGAAANAGCTATGACCGCTTCTATATGAGCGCATCTCACCTGAATTTG1900.14218683349921798No Hit
CAGGAAANAGCTATGANCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG1890.14143848174395895No Hit
CAGGAAACAGCTATGACCGCAGCAAGAGACCCAGACCCACCCGACTGGCC1880.14069012998869987No Hit
CAGGAAACAGCTATGACCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC1870.13994177823344084No Hit
CAGGAAANAGCTATGANNGGGCAGGCCGAGGACATTAGAAATTTGGGACG1860.1391934264781818No Hit
CAGGAAANAGCTATGACCAAGGACAAGGTGAGGCAGCAAGTGCTGATGTA1850.13844507472292278No Hit
CAGGAAANAGCTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG1850.13844507472292278No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG1840.13769672296766372No Hit
CAGGAAANAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT1830.13694837121240466No Hit
CAGGAAANAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC1830.13694837121240466No Hit
CAGGAAACAGCTATGACCGTCAACAGCATACCTGTACACCTGAAATAACT1790.13395496419136851No Hit
CAGGAAANAGCTATGANCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG1790.13395496419136851No Hit
CAGGAAANAGCTATGANCCGCTTCTGGGGACTTTGCTCTTGGGCTCTTTT1760.13170990892559137No Hit
CAGGAAACAGCTATGACCTCCTCTTCATTTTCACGGTGCCATTCTGATTC1750.13096155717033234No Hit
CAGGAAANAGCTATGACCACAGGGGCCTCTTAAAACTGCATGTGTTCAGC1750.13096155717033234No Hit
CAGGAAANAGCTATGANCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT1750.13096155717033234No Hit
CAGGAAACAGCTATGACCTCCTCTTCATTTTCACGGTGCCATTCCGATTC1680.12572309488351904No Hit
CAGGAAANAGCTATGANNCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT1680.12572309488351904No Hit
CAGGAAANAGCTATGANCTCCTCTTCATTTTCACGGTGCCATTCTGATTC1670.12497474312826001No Hit
CAGGAAACAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG1660.12422639137300096No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGGCTCTTTT1650.12347803961774192No Hit
CAGGAAANAGCTATGANCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC1630.12198133610722385No Hit
CAGGAAANAGCTATGANNACCGTTAAGCCACACCAAATATGAATTTCTGT1620.1212329843519648No Hit
CAGGAAANAGCTATGANCGTCAACAGCATACCTGTACACCTGAAATAACT1580.11823957733092863No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT1570.1174912255756696No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1550.1159945220651515No Hit
CAGGAAANAGCTATGANCCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG1540.11524617030989245No Hit
CAGGAAANAGCTATGANNGCATCACTGAGGGAGGATCCTCGGGTCTTACA1470.11000770802307917No Hit
CAGGAAACAGCTATGACCGCAGCAAGAGACCAATACCGCATTGTAGTCAA1470.11000770802307917No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT1460.10925935626782013No Hit
CAGGAAANAGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1450.10851100451256107No Hit
CAGGAAANAGCTATGACCTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC1420.10626594924678395No Hit
CAGGAAANAGCTATGACCACTCCTCCTGGCCTTCCTCTTTCAGAGATGGT1420.10626594924678395No Hit
NAGGAAANAGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1410.10551759749152492No Hit
CAGGAAANAGCTATGANCGGCTGGCACAAAGAACAGCACTGACTGTTTAT1410.10551759749152492No Hit
CAGGAAANAGCTATGANCTCCTCTTCATTTTCACGGTGCCATTCCGATTC1400.10476924573626588No Hit
CAGGAAANAGCTATGANCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT1400.10476924573626588No Hit
CAGGAAACAGCTATGANCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT1380.10327254222574779No Hit
CAGGAAACAGCTATGACCCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG1370.10252419047048875No Hit
CAGGAAACAGCTATGACCGGCTGGCACAAAGAACAGCACTGACTGTTTAT1360.10177583871522972No Hit
CAGGAAANAGCTATGACCGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG1360.10177583871522972No Hit
NAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1350.10102748695997067No Hit
CAGGAAACAGCTATGANCAGGCACCAACAGTACAAAGCAGATACAAAGCA1350.10102748695997067No Hit
CAGGAAANAGNTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA1340.10027913520471161No Hit
CAGGAAANAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGAGCCT1340.10027913520471161No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)