FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92687
Sequences flagged as poor quality0
Sequence length300
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG18872.0358842124569785No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT18041.9463355163075728No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT16921.825498721503555No Hit
CAGGAAACAGCTATGACCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT16701.8017629225241945No Hit
CAGGAAACAGCTATGACCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC16691.8006840225705871No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT14311.543905833612049No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAACT13921.5018287354213644No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT13471.4532782375090358No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAATT13431.4489626376946068No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA13151.418753438993602No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGCACTCCTTC12331.3302836427978033No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC12331.3302836427978033No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCTGCCCAACACCC11681.2601551458133287No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT10931.1792376492927812No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA10541.1371605511020964No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC10181.0983201527722335No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT10091.0886100531897678No Hit
CAGGAAACAGCTATGACCGGGGAGACTGGGCCATACGATAGGCTTAATCC10061.0853733533289458No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTGTTT9240.9969035571331472No Hit
CAGGAAACAGCTATGACCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT9230.9958246571795398No Hit
CAGGAAACAGCTATGACCGGCGGGCCCTGATATAGAGCCACTGGCCGTCG8830.9526686590352477No Hit
CAGGAAACAGCTATGACCCATGGTTGGGGAAAAACTCCTCATCTTCATGT8560.9235383602878505No Hit
CAGGAAACAGCTATGACCAGCGTCTCATTCCATAAAGGCCACCATGAAAC8270.8922502616332386No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG8200.8846979619579877No Hit
CAGGAAACAGCTATGACCAGCAACATACCGTTTGTAAACAACTGGCCGTC8180.882540162050773No Hit
CAGGAAACAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT7840.8458575636281248No Hit
CAGGAAACAGCTATGACCTCCCACCACTGACTCCAGGGGAAGGAGGAGGA7780.8393841639064809No Hit
CAGGAAACAGCTATGACCGATGTAGCAGTGCTCTGGAGTCCTCCCTTGCT7660.8264373644631933No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC7510.8102538651590838No Hit
CAGGAAACAGCTATGACCCATGGTTGGGGAAAAACTCCTCATTTTCATGT7410.7994648656230109No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA7360.7940703658549743No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG6920.746598767896253No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA6880.7422831680818238No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTGCAGTGAACTGA6830.7368886683137873No Hit
CAGGAAACAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG6770.7304152685921435No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG6280.6775491708653857No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG6110.6592078716540616No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTCAGGAATCATGC5900.6365509726283082No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGACCGGGATTCGTG5130.5534756762005459No Hit
CAGGAAACAGCTATGACCTCTACGCCCCTCAGCTGTGTCCTGTCTGCATA5130.5534756762005459No Hit
CAGGAAACAGCTATGACCCTTGGTGGAGCCATAGGCCGCATGCACGCGCA4980.5372921768964364No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA4850.5232664774995415No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCATA4750.5124774779634684No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG4720.5092407781026465No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG4610.49737287861296625No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG4510.4865838790768932No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA4500.4855049791232859No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCAGCACAGGGAGT4450.4801104793552494No Hit
CAGGAAACAGCTATGACCCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA4330.4671636799119617No Hit
CAGGAAACAGCTATGACCTGCACACACCTCCTATGTTAACAGGCACAAGT4270.460690280190318No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACCCTGTCTCTCTAGC4250.45853248028310334No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT4240.457453580329496No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC4210.4542168804686742No Hit
CAGGAAACAGCTATGACCGGGCATGGTGTGGGACTAGCTAATCTAGGGAC4080.4401911810717792No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGCAGGGGCATCCGG4050.43695448121095726No Hit
CAGGAAACAGCTATGACCAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC3980.4294021815357062No Hit
CAGGAAACAGCTATGACCTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT3940.425086581721277No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAACAGTGAAGACAG3790.4089030824171675No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTTGCAGTGAGCCG3780.40782418246356017No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAACAGCAGACACC3740.4035085826491309No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCCGGAGAGCCTGT3730.4024296826955236No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC3580.3862461833914141No Hit
CAGGAAACAGCTATGACCTGGCATCCAGGGCCTATTTGGTTCCTATTGAG3560.38408838348419955No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT3480.3754571838553411No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCCCGTGTCAAGC3460.3732993839481265No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA3320.3581947845976242No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCGGTACTGGCAGT3180.34309018524712204No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATGAGTTAGCCAGC3080.332301185711049No Hit
CAGGAAACAGCTATGACCTCCTGCCCCACCCTGATACAGGTCACTGTGGA3010.3247488860357979No Hit
CAGGAAACAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT2920.31503878645333216No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGCCCCTGTACC2900.31288098654611757No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGGTCCTCCTC2870.30964428668529564No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA2860.3085653867316884No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAGGCACTACGT2810.30317088696365185No Hit
CAGGAAACAGCTATGACCAAACTATTAATACCTGCAGCAAAGCCCCAAGC2810.30317088696365185No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCTTCCCATCTCA2750.29669748724200806No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCGCCCAACACCC2750.29669748724200806No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGGGACATGGCC2740.29561858728840074No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTGCTTGAGTCTGG2590.27943508798429123No Hit
CAGGAAACAGCTATGACCGCATCCTGCTTAGTGCTGCCTCAATCTGGTCC2530.2729616882626474No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT2530.2729616882626474No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAACTGGCCAGCCTG2520.2718827883090401No Hit
CAGGAAACAGCTATGACCTGACTGAAAGGCTTTTCCACGTGCACTGCTGG2500.2697249884018255No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGATTCTCAGATTCC2470.2664882885410036No Hit
CAGGAAACAGCTATGACCCCCTGCGGCTCCTTTCAAGTTAAGTCACCACT2270.24491028946885757No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCCTCCCCACCTCC2250.24275248956164294No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAATGAAACTTGGTT2180.23520018988639185No Hit
CAGGAAACAGCTATGACCCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG2150.2319634900255699No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGCCGCCCCCCTGCA2150.2319634900255699No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCCATGCCTGCCGT2090.2254900903039261No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGACGACAGGAAAA2070.22333229039671149No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCAGACCAATGCC2030.2190166905822823No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTGGCAGCTGCAC2020.21793779062867502No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGTCTGCAGCCTGG1970.2125432908606385No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTAGTGGCATGAA1940.2093065909998166No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA1930.2082276910462093No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCAGGCCCTGGCGT1900.20499099118538736No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGCCCCGGACGAGG1880.2028331912781728No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATTCTCCATCTCCA1860.20067539137095816No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGACACCTCCTCTGG1850.1995964914173509No Hit
CAGGAAACAGCTATGACCTGCTGGGAGAGGGGGATGAGTAGACAGGGCCA1840.19851759146374356No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA1820.19635979155652894No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT1820.19635979155652894No Hit
CAGGAAACAGCTATGACCTTGGACAGCACCTTGAATGCTATCAGGGAAGC1810.19528089160292167No Hit
CAGGAAACAGCTATGACCATCGCACCACTGCACTCCAGCGCTCCATACAC1740.18772859192767055No Hit
CAGGAAACAGCTATGACCTCCAGTAAAACTGCCTCTGTTTCAACTCCCTT1700.18341299211324136No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTGGCTGCTT1660.1790973922988121No Hit
CAGGAAACAGCTATGACCTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG1640.1769395923915975No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTTAGGAATCATGC1630.17586069243799024No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATGGTGAGCCCTGC1620.17478179248438291No Hit
CAGGAAACAGCTATGACCTGGGTTGCCTGGGACTGAGAGGGTCCTGGGAC1610.17370289253077562No Hit
CAGGAAACAGCTATGACCGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC1600.17262399257716832No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT1570.16938729271634642No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTTGGCATGAAGG1570.16938729271634642No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTGAGTGAGCCATG1560.1683083927627391No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGCAGGTCGTGCTG1530.1650716929019172No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGCCATGGGGACTG1530.1650716929019172No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCCTGGCTTGTC1510.1629138929947026No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT1480.1596771931338807No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACAGTCTCCCCAGG1460.15751939322666608No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCATGCTGGGTGGGA1450.15644049327305878No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCAGGAAACAGCTA1450.15644049327305878No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGGGGGCCTGGCT1450.15644049327305878No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG1440.15536159331945149No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGCTGGTGTGGGGA1440.15536159331945149No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGGTGGGGACTGGA1400.1510459935050223No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA1380.14888819359780767No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGTTGGGGACTGGA1330.14349369382977117No Hit
CAGGAAACAGCTATGACCTGCCCAATAGTTCTTGCCTCCAAAATTATCCT1310.14133589392255655No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGCAGCCGCTGCTG1290.13917809401534195No Hit
CAGGAAACAGCTATGACCATCGCACCACTGCACTCCAGCACTGGCCGTCG1280.13809919406173465No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACGCCTGCTGGGTC1280.13809919406173465No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACACACACTTCTAA1250.13486249420091276No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTTCATTCT1240.13378359424730546No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGACTCTCAGATCCC1210.13054689438648354No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTCCATTCT1180.1273101945256616No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACAGAGGAGCCGGT1170.12623129457205434No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCTCTGCAGCTAGC1150.12407349466483973No Hit
CAGGAAACAGCTATGACCTCCACAAGGCCCTGGGACAATCTCCCAGGCCC1140.12299459471123242No Hit
CAGGAAACAGCTATGACCTCCAGTGGTGGGGCTCAACAAAGGCTCCGTGA1130.12191569475762513No Hit
CAGGAAACAGCTATGACCCCCATCCACTAGTCGGGGAGTTGCTTGTCATT1110.11975789485041052No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTGTAATCCCAGCT1100.11867899489680321No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCCGTCCTCCCAGC1080.11652119498958861No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATGGAGCCCTGCAG1070.11544229503598132No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCAGGAAACAGCT1060.114363395082374No Hit
CAGGAAACAGCTATGACCTGCAATCTTGAGCTCCTGGGCTCAGGTGATCT1050.11328449512876672No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCCCTTCCTTACCT1050.11328449512876672No Hit
CAGGAAACAGCTATGACCTCTACGCCCCTCAGCTGTGTCCTATCTGCATA1040.1122055951751594No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAATAGTCT1040.1122055951751594No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAAGGCACTACG1030.11112669522155211No Hit
CAGGAAACAGCTATGACCCCCATCCACTAGTCGGGGAGTGTCATTGGTGC1020.1100477952679448No Hit
CAGGAAACAGCTATGACCACCCAGTTAGAGCCATGGTTCCTGTAGGCTAG1010.10896889531433751No Hit
CAGGAAACAGCTATGACCATCGCACCACTGCACTCCAGCAGGAAACAGCT980.1057321954535156No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCCGCTCAGCTCC980.1057321954535156No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGGCTGGAAACTGG980.1057321954535156No Hit
CAGGAAACAGCTATGACCTTCCTGATGGGATTCAGCCATCAACAGTAGGA970.1046532954999083No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACTAACAAACTCCC950.10249549559269368No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCACTCCCCAGGC940.1014165956390864No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTGGCATGAAGGA930.10033769568547908No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCCGCATA930.10033769568547908No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)