FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116769
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT17681.514100488999649No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT15361.3154176194024099No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA12991.1124527914086788No Hit
CAGGAAANAGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC12121.0379467153097142No Hit
CAGGAAANAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT11921.0208188817237451No Hit
CAGGAAANAGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT11731.0045474398170746No Hit
CAGGAAANAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT10940.9368924971524978No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT10670.9137699218114397No Hit
CAGGAAANAGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA9990.8555352876191455No Hit
CAGGAAANAGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC9340.7998698284647465No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT8620.7382096275552588No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT8190.7013847853454256No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT7490.6414373677945345No Hit
CAGGAAANAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA7450.6380118010773407No Hit
CAGGAAANAGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA7360.6303042759636547No Hit
CAGGAAANAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT7270.6225967508499688No Hit
CAGGAAANAGCTATGACCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC7220.6183147924534765No Hit
CAGGAAANAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT6710.5746388168092559No Hit
CAGGAAANAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT6480.5549418081853916No Hit
CAGGAAANAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA6320.5412395413166166No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCAGAGGGAAGGTGACT6050.5181169659755586No Hit
CAGGAAANAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT6010.5146913992583648No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTGGCCGTC5780.4949943906345006No Hit
CAGGAAANAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC5380.4607387234625628No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA5340.4573131567453691No Hit
CAGGAAANAGCTATGANNTGAAGTGGCTCCATCCTTCCATTTGTTTATAT5110.43761614812150484No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA4850.4153499644597453No Hit
CAGGAAANAGCTATGANNGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT4850.4153499644597453No Hit
CAGGAAACAGCTATGACCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC4820.4127807894218499No Hit
CAGGAAANAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT4740.40592965598746245No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT4710.40336048094956706No Hit
CAGGAAANAGCTATGANNTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG4710.40336048094956706No Hit
CAGGAAANAGCTATGANCAGGCACCAACAGTACAAAGCAGATACAAAGCA4510.3862326473635982No Hit
CAGGAAANAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA4440.3802379056085091No Hit
CAGGAAANAGCTATGANCTGGGGATGAGGACAGCAGGGCAGTCACCATCT4390.3759559472120169No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTATGTGCA4160.3562589385881527No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA4140.3545461552295558No Hit
CAGGAAANAGCTATGACCACCAAGACCCCAGCAGCAGAGGGAAGGTGACT4070.34855141347446666No Hit
CAGGAAANAGCTATGACCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT3980.34084388836078067No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT3940.3374183216435869No Hit
CAGGAAANAGCTATGANCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC3870.3314235798884978No Hit
CAGGAAANAGCTATGANNCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC3820.3271416214920056No Hit
CAGGAAANAGCTATGANCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC3800.32542883813340867No Hit
CAGGAAANAGCTATGANCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT3750.32114687973691647No Hit
CAGGAAANAGCTATGANCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA3630.31087017958533514No Hit
CAGGAAANAGCTATGACCTGAAGTGGCTCCATCCTTCCATTTGTTTATAT3490.29888069607515694No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC3470.29716791271656007No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT3460.2963115210372616No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA3450.29545512935796314No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC3440.29459873767866473No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA3420.2928859543200678No Hit
CAGGAAANAGCTATGACCTTCATACAGAGATACAAAGGCAACTGGCCGTC3380.289460387602874No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA3250.2783272957719943No Hit
CAGGAAANAGCTATGANNCTGGCCTCCTTCAATGCATTTAATGGCCTTAT3190.2731889456962036No Hit
CAGGAAANAGCTATGANNTGCCCAGTTGTTTCCAGAACCTTGTTTTTGAC3160.2706197706583083No Hit
CAGGAAANAGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT3080.26376863722392074No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC3040.26034307050672695No Hit
CAGGAAANAGCTATGANCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT2990.25606111211023475No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2850.24407162860005652No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA2780.23807688684496742No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT2750.23550771180707208No Hit
CAGGAAANAGCTATGANCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA2690.2303693617312814No Hit
CAGGAAANAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG2670.22865657837268455No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTGGCCGTC2660.22780018669338611No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCAGAGGGAAGGTGACT2650.22694379501408765No Hit
CAGGAAANAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA2600.22266183661759542No Hit
CAGGAAANAGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT2500.214097919824611No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA2460.21067235310741722No Hit
CAGGAAANAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA2410.20639039471092496No Hit
CAGGAAANAGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT2340.2003956529558359No Hit
CAGGAAANAGCTATGANCACCAAGACCCCAGCAGCAGAGGGAAGGTGACT2320.198682869597239No Hit
CAGGAAANAGCTATGANCTTCATACAGAGATACAAAGGCAACTGGCCGTC2220.19011895280425456No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA2210.18926256112495612No Hit
CAGGAAANAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2170.18583699440776233No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC2140.183267819369867No Hit
CAGGAAACAGCTATGACCTGAAGTGGCTCCATCCTTCCATTTGTTTATAT2120.18155503601127013No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT2100.17984225265267323No Hit
CAGGAAANAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC2080.17812946929407636No Hit
CAGGAAANAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA2060.17641668593547946No Hit
CAGGAAANAGCTATGANNAAGTTGGTCAGCCCTGGTGACTCATTGGATAT2050.175560294256181No Hit
CAGGAAANAGCTATGANNGCTGCTGACCCAACCCTACCCTGTCCAGCCCC2030.1738475108975841No Hit
CAGGAAANAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC2030.1738475108975841No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA2020.17299111921828567No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGGAACACACAACTGGC2020.17299111921828567No Hit
CAGGAAANAGCTATGACCCTGGCCTCCTTCAATGCATTTAATGGCCTTAT1980.1695655525010919No Hit
CAGGAAANAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC1970.16870916082179346No Hit
CAGGAAANAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1910.1635708107460028No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC1900.16271441906670434No Hit
CAGGAAANAGCTATGANNTGTTGTCTTTCAGGGGCCGCCATGCCTGCCGT1890.1618580273874059No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGGAACACACAACTGGC1880.16100163570810747No Hit
CAGGAAANAGCTATGANCTTCATACAGAGATACAAAGGCAACTATGTGCA1840.15757606899091367No Hit
CAGGAAANAGCTATGANCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT1840.15757606899091367No Hit
CAGGAAANAGCTATGACCTGCCCAGTTGTTTCCAGAACCTTGTTTTTGAC1830.15671967731161524No Hit
CAGGAAANAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1830.15671967731161524No Hit
CAGGAAACAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG1810.15500689395301837No Hit
CAGGAAANAGCTATGANCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1770.15158132723582457No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1730.1481557605186308No Hit
CAGGAAANAGCTATGANNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT1710.1464429771600339No Hit
CAGGAAANAGCTATGANNGCCCTGGCCACTGTGGTTAATGAAACTTGGTT1710.1464429771600339No Hit
CAGGAAANAGCTATGANCTGAAGTGGCTCCATCCTTCCATTTGTTTATAT1680.14387380212213857No Hit
CAGGAAACAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1670.14301741044284014No Hit
CAGGAAANAGCTATGANNCTTCCATGCTGGGCTCTGGACTGGCCGTCGTT1630.13959184372564637No Hit
CAGGAAANAGCTATGANCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG1610.13787906036704947No Hit
CAGGAAANAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC1600.13702266868775104No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1570.1344534936498557No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA1560.13359710197055724No Hit
CAGGAAANAGCTATGANNCTGGCCTCCTTCAATGCATTTAATGGCCTTCT1550.1327407102912588No Hit
CAGGAAANAGCTATGANNTGGCATCCAGGGCCTATTTGGTTCCTATTGAG1510.12931514357406504No Hit
CAGGAAANAGCTATGANCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1510.12931514357406504No Hit
CAGGAAANAGCTATGANCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC1510.12931514357406504No Hit
CAGGAAANAGCTATGANCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA1500.12845875189476658No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1500.12845875189476658No Hit
CAGGAAANAGCTATGANNGCTACGGGCAATCACTCTCCTACTCCCAACCA1490.12760236021546814No Hit
CAGGAAANAGCTATGANNTGCCAAAATTAAGGATGCACACCTGCCTCACT1480.1267459685361697No Hit
CAGGAAANAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC1470.12588957685687127No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC1470.12588957685687127No Hit
CAGGAAACAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT1450.12417679349827437No Hit
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1440.12332040181897592No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGGAACACACAACTGGC1410.12075122678108059No Hit
CAGGAAANAGCTATGANCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC1400.11989483510178216No Hit
CAGGAAANAGCTATGANNGCAGCAAGAGACCAATACCGCATTGTAGTCAA1360.11646926838458838No Hit
CAGGAAANAGCTATGANNAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT1340.11475648502599148No Hit
CAGGAAANAGCTATGANNTGCCAAAATTAAGGATGCACACCTGTGACACA1340.11475648502599148No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1310.11218730998809616No Hit
CAGGAAANAGCTATGANCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1300.11133091830879771No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC1300.11133091830879771No Hit
CAGGAAANAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT1300.11133091830879771No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1290.11047452662949926No Hit
CAGGAAANAGCTATGANNGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT1280.10961813495020081No Hit
NAGGAAANAGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1280.10961813495020081No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1270.10876174327090236No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC1240.10619256823300705No Hit
CAGGAAANAGCTATGANNGTGCAGTCCTCAGGCCACACTAGAAGTAATTA1230.10533617655370861No Hit
CAGGAAANAGCTATGANCCTGGCCTCCTTCAATGCATTTAATGGCCTTAT1210.10362339319511171No Hit
CAGGAAANAGCTATGANCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC1200.10276700151581326No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG1190.10191060983651484No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG1190.10191060983651484No Hit
CAGGAAANAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT1190.10191060983651484No Hit
CAGGAAANAGCTATGANNGCAGCTCCCCACAACTTCAACTGGCCGTCGTT1180.1010542181572164No Hit
CAGGAAANAGCTATGANCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1180.1010542181572164No Hit
NAGGAAANAGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1170.10019782647791795No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)