FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152284
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG85435.609913057182633No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG70014.597331302040923No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC68314.485697775209477No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG42752.807254865908434No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG41002.6923380000525334No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG30752.0192535000394No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC30001.9700034146725856No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC29391.9299466785742427No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA29341.9266633395497885No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC26411.734259672716766No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGTGAACGAAG25991.7066796249113498No Hit
TGTAAAACGACGGCCAGTTCCTCCTTTGCCAATATAGGGTTTGATGTAAT24871.6331328307635733No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC24121.5838827453967586No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCATCAGGTTCCC23621.5510493551522155No Hit
TGTAAAACGACGGCCAGTACATGCGTGGCCCTGTCTACGGCCCCGCCCTC19971.3113656063670511No Hit
TGTAAAACGACGGCCAGTGGGGTGGGGAGTGAGATGGTCCTAGGAAGTAC16951.1130519292900107No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA16511.0841585458748129No Hit
TGTAAAACGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGGGACACGGA16271.068398518557432No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCTGGAGGGCAGCCA16181.0624885083134143No Hit
TGTAAAACGACGGCCAGTATCTGCACATCGTGCTGCCACACGAGGCTTCA15471.0158650941661633No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCTGGAGGGCGGCCA14260.936408289774369No Hit
TGTAAAACGACGGCCAGTGGCTCTCATCCTCTTCTGCAACTACTATGTTT13760.9035748995298258No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCCT12860.8444747970896483No Hit
TGTAAAACGACGGCCAGTTGGATGGTGAGGGAAGGGCTAAGACAGGATTC12270.8057313966010874No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT12170.7991647185521789No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC11590.7610779858685088No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT10610.6967245409892043No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG10570.6940978697696409No Hit
TGTAAAACGACGGCCAGTAGGAGGGCGGAGGAGGAAAGCAGGTTTGCTCT10570.6940978697696409No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG10490.688844527330514No Hit
TGTAAAACGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT9860.6474744556223898No Hit
TGTAAAACGACGGCCAGTGCAGAAGATGGACTGTGGAGGGGGCTACATTA9140.6001943736702476No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC8340.5476609492789788No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC8170.5364975965958341No Hit
TGTAAAACGACGGCCAGTCCTGTGTTTCCACTTCTGCTTCCAAAGATTTT7140.4688608126920753No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA6890.45244411756980385No Hit
TGTAAAACGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC6480.42552073756927844No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA6280.4123873814714612No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCCTGCCTCTCCCAGG5910.3880906726904993No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGGGGCCCGGATGT5030.3303039058601035No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT4890.32111055659163146No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC4520.29681384781066955No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCGCCTCGGCCTCC4220.2771138136639437No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCCAAGAGGTGGGG4160.2731738068345985No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCCTGCCTGTCCCAGG4150.27251713902970764No Hit
TGTAAAACGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG3700.24296708780961887No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGCATTTCCTTGGC3410.22392372146778386No Hit
TGTAAAACGACGGCCAGTAGGCTTTGGGAGGCCATGACTAGTTAGAGACA3380.2219537180531113No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG3220.2114470331748575No Hit
TGTAAAACGACGGCCAGTGCCATCGGCTCCCAAGTTTTATGTCCTAGAGC3190.20947702976018492No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG3180.20882036195529405No Hit
TGTAAAACGACGGCCAGTACACATTCTTGTAAAGCATGGGATTGCTGAAT3150.20685035854062145No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCAAGAGGTGGGGT2880.1891203278085682No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGTGCCTG2840.18649365658900474No Hit
TGTAAAACGACGGCCAGTGACCTCATGGGGCGCTTCCAGCCTCAGGGTCG2590.1700769614667332No Hit
TGTAAAACGACGGCTAGTTGGAACCATTTTTGATAACTTTCTGATCACAG2480.1628536156129337No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGCGCCTG2400.15760027317380684No Hit
TGTAAAACGACGGCCAGTAGATCATCCAGCGCACACGGCGTCTTGGCGGG2380.1562869375640251No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCACATGCCTCCC2350.15431693414935255No Hit
TGTAAAACGACGGCCAGTGGATGCCAAGGCAGTACCTGAAGCACCGGGGA2220.14578025268577133No Hit
TGTAAAACGACGGCCAGTTGGAACCATTTTTGACAACTTTCTGATCACAG2180.14315358146620788No Hit
TGTAAAACGACGGCCAGTGGATGCCAAGGCAGTACCTGAAGCACCAGGGA2160.14184024585642616No Hit
TGTAAAACGACGGCCAGTCTTGCACTACATGGCCATCAGGGTCTCGTCCC2110.13855690683197186No Hit
TGTAAAACGACGGCTAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG2020.1326468965879541No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCGTCTACACGAGA2010.13199022878306324No Hit
TGTAAAACGACGGCCAGTTGGAACCATTTTTGATACCTTTCTGATCACAG1990.13067689317328152No Hit
TGNAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGCGAGACGGGCACC1990.13067689317328152No Hit
TGTAAAACGACGGCTAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC1980.13002022536839064No Hit
TGTAAAACGACGGCCAGTCTTGCACTACATGGCCATCATGAACCAGCGAA1960.12870688975860892No Hit
TGNAAAACGACGGCCAGTTGGAACCATTTTTGATAACCTTCTGATCACAG1890.1241102151243729No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCACCTTGGCCTCC1810.11885687268524599No Hit
TGNAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG1750.11491686585590082No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACCGTGAGACGGGCACC1610.10572351658742875No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC1580.10375351317275616No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCACAGCACTGCC1560.10244017756297444No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)