FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133711
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA30932.3131978670416045No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC29862.2331745331348953No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC29172.1815707009894476No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC28592.138193566722259No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT28382.1224880525910357No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC27962.091077024328589No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA27832.0813545631997368No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA26822.0058185190448055No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCATA22841.7081616321768591No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT22801.7051701056756736No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA22751.701430697549192No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA22121.6543141551555218No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCAGGGCAGCGGT21811.6311298247713353No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG20871.5608289519934786No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA20431.5279221604804392No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC17811.331977174652796No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC16791.2556932488725685No Hit
CAGGAAACAGCTATGACCAGATCCTGTGAAGTGGGAGGGTTTTCAAAGTC16671.2467186693690122No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG16441.2295173919871962No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT16191.2108203513547875No Hit
CAGGAAACAGCTATGACCGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC16071.2018457718512314No Hit
CAGGAAACAGCTATGACCTGCTGGGAGAGGGGGATGAGCGGCGCCGCCAT14961.1188309114433368No Hit
CAGGAAACAGCTATGACCGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG13070.9774812842623268No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT12300.9198943991145082No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA12190.9116677012362483No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA12190.9116677012362483No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT12100.9049367666085812No Hit
CAGGAAACAGCTATGACCGGGTCCCACCCATGCTGAAAGTCCACCTTCCA11860.8869876076014688No Hit
CAGGAAACAGCTATGACCTGACTGCAAACATAGAAGTGAGCAGAGCAAGA11460.8570723425896148No Hit
CAGGAAACAGCTATGACCTGACAGCACAAGGAACACTGACCTACAGATAA10910.8159388531983157No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC10340.773309600556424No Hit
CAGGAAACAGCTATGACCGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT9970.7456379804204591No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCCAGCTAAGTGA9530.7127311889074197No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC8930.6678582913896389No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCCAGGCTGGT8920.6671104097643425No Hit
CAGGAAACAGCTATGACCTCCCTTAAACTTGGGATTGCTCCATTGCACTC8650.6469176058813411No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCTGAATAGTTGT8560.640186671253674No Hit
CAGGAAACAGCTATGACCTGACTTTTGCAAGTCACAGAGAACTGTTGTAT8500.6356993815018959No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC8240.6162544592441908No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCAAGTGCTGATGTA7980.5968095369864858No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC7600.5683900352252245No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTGATCCCCCAAAA7370.5511887578434085No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTGACACA7060.5280044274592217No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT6710.5018285705738496No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT6640.49659339919677514No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGGCACGGGAACA6340.4741569504378847No Hit
CAGGAAACAGCTATGACCGCAGCAAGAGACCAATACCGCATTGTAGTCAA5670.42404888154302933No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAATAGTCT5570.41657006529006585No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCTATTTTCCAAAG5450.4075954857865097No Hit
CAGGAAACAGCTATGACCTCCAGCCAGCCAGTCCTTGTAACTCAGCATGA5450.4075954857865097No Hit
CAGGAAACAGCTATGACCTTGCCAGTGCTGCCTTCAGCCATTTGTGAAAT5360.4008645511588426No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTTGTAATCCCATC4990.3731929310228777No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCCAGAGGGGTC4880.3649662331446179No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCAGGAAACAGCT4720.3530001271398763No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCGGGTCTTACA4490.3357988497580603No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCAGAGAGTGATAT4190.31336240099916984No Hit
CAGGAAACAGCTATGACCCCTGACCCCTTCTTCTCCAGGAAACTCTCTTC4170.31186663774857715No Hit
CAGGAAACAGCTATGACCCCTGACCCCTTCTTCTCCAGGAAACTCTGTTC4040.30214417661972465No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC3970.29690900524265024No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTTTCAGAGATGGT3460.25876704235253645No Hit
CAGGAAACAGCTATGACCACTCCCTTGAGCCTTTGGGGGCCACTGCAGCT3390.253531870975462No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC3380.25278398935016566No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC3300.24680093634779487No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCATGTGTTCAGC3280.24530517309720215No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCATGTGTTCAGT3240.24231364659601679No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT2940.21987719783712634No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCACGGTGGCTCAT2880.21538990808534828No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC2840.21239838158416285No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCCACTCCTCCCCC2780.2079110918323848No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTGTAATCCCAGCT2610.19519710420234684No Hit
CAGGAAACAGCTATGACCGGCCTTCTCTGCATTATCTCGCTCATAAGAGT2600.1944492225770505No Hit
CAGGAAACAGCTATGACCCCCATCCACTAGTCGGGGAGTTACACAAACAG2520.1884661695746797No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTTGTAGTCCCAGC2470.18472676144819797No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA2450.18323099819760527No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGAAGCTCCAGCA2360.17650006356993814No Hit
CAGGAAACAGCTATGACCACCCCTTGCATTGGTAGGGCTTCTCTCCTGTG2290.17126489219286373No Hit
CAGGAAACAGCTATGACCGGCGGGCCCTGATATAGAGCAGGCGCCGCGGG2260.16902124731697465No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCAGACACACGGC2180.1630381943146039No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCGCAGTGGCTGAT2160.16154243106401117No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG2060.1540636148110477No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCTGCTGATTCCAC1980.14808056180867693No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1950.14583691693278786No Hit
CAGGAAACAGCTATGACCAGCAGCTTTCAAACCTAGACACTTTATTGTTT1850.13835810067982438No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG1790.1338708109280463No Hit
CAGGAAACAGCTATGACCAGGAGAAGCAAAGGACAGATCACAAAATTGAT1600.1196610600474157No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCTGGGTGCTGGTG1580.118165296796823No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG1580.118165296796823No Hit
CAGGAAACAGCTATGACCGCTGAGGCAGGACAATCGCTTGAACTTGGGAG1530.11442588867034126No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGTCTGGGTCCT1460.10919071729326682No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1450.10844283566797047No Hit
CAGGAAACAGCTATGACCCAGTTGCTTGGGGTTTGTGTCTTATGATGTTT1410.10545130916678508No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCCTCTCCCAACT1370.10245978266559969No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)