FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93396
Sequences flagged as poor quality0
Sequence length300
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT10641.1392350850143476No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT8780.94008308707011No Hit
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG8530.9133153454109384No Hit
CAGGAAACAGCTATGACCTGGAAACCCATGCCATGCACTCAACACTACAA8430.9026082487472697No Hit
CAGGAAACAGCTATGACCGTCTCAGGGATCACGTGGCGCAGACCTCGCTC7530.8062443787742516No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCTGAGGCAGGAGA6690.7163047667994347No Hit
CAGGAAACAGCTATGACCTCCTGCCCCACCCTGATACAGGTCACTGTGGA6430.6884663154738961No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA6410.6863248961411623No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC5570.5963852841663455No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA5550.5942438648336117No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG5170.5535568975116707No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCTGCCCAACACCC5120.5482033491798364No Hit
CAGGAAACAGCTATGACCAGGTTGCTGGATTCAGTTTGCTGACCAACTTT5120.5482033491798364No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCAGGAAACAGCT5070.542849800848002No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT4920.5267891558524991No Hit
CAGGAAANAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT4730.5064456721915286No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCAAGCCTGACTG4710.5043042528587948No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCTGGGTGCTGGTG4590.4914557368623924No Hit
CAGGAAANAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT4460.4775365111996231No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT4380.46897083386868815No Hit
CAGGAAANAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG4130.4422030922095165No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAGGCCGCAGCTCC4080.43684954387768216No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT3650.39080902822390684No Hit
CAGGAAANAGCTATGACCGTCTCAGGGATCACGTGGCGCAGACCTCGCTC3620.3875968992248062No Hit
CAGGAAANAGCTATGACCTGGAAACCCATGCCATGCACTCAACACTACAA3620.3875968992248062No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC3540.3790312218938713No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAACGCAGCTCCCCA3510.37581909289477067No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC3460.3704655445629363No Hit
CAGGAAACAGCTATGACCTGACTGAAAGGCTTTTCCACGTGCACTGCTGG3400.3640412865647351No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG3360.35975844789926764No Hit
CAGGAAANAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA3340.35761702856653393No Hit
CAGGAAACAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT3300.3533341899010664No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG3200.34262709323739776No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA3190.34155638357103085No Hit
NAGGAAANAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG3040.3254957385755279No Hit
CAGGAAANAGCTATGACCTCCTGCCCCACCCTGATACAGGTCACTGTGGA2990.32014219024369356No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT2990.32014219024369356No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGGAGTCAC2980.31907148057732665No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA2910.31157651291275856No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT2900.3105058032463917No Hit
CAGGAAANAGCTATGACCAATGCAAGGGACCACCAGGCTGAGGCAGGAGA2790.29872799691635615No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2760.2955158679172556No Hit
CAGGAAACAGCTATGACCGGCGGGCCCTGATATAGAGCCACTGGCCGTCG2730.292303738918155No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCCCCGGGCAGA2700.28909160991905436No Hit
CAGGAAANAGCTATGACCGCTGCTGACCCAACCCTACCCAGGAAACAGCT2660.2848087712535869No Hit
CAGGAAANAGCTATGACCGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA2570.2751723842562851No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGACACTGCAGCCTGGG2530.2708895455908176No Hit
CAGGAAANAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2500.267677416591717No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCAGGCCCTGGCGT2450.26232386825988263No Hit
CAGGAAANAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT2440.2612531585935158No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT2410.2580410295944152No Hit
CAGGAAANAGCTATGACCACAGGAGACAGGATTGGGGCTGGGTGCTGGTG2380.25482890059531454No Hit
NAGGAAANAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT2360.25268748126258084No Hit
CAGGAAANAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT2350.25161677159621393No Hit
CAGGAAANAGCTATGACCAGGTTGCTGGATTCAGTTTGCTGACCAACTTT2320.24840464259711337No Hit
CAGGAAANAGCTATGACCAACACGGCAGTCAGAGGTGCTGCCCAACACCC2320.24840464259711337No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT2280.24412180393164593No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCCTCCCCACCTCC2210.23662683626707784No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGTCTGGGTCCT2190.23448541693434408No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGTGGAGACGGC2190.23448541693434408No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT2180.23341470726797722No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACGGAGATCCAGCC2180.23341470726797722No Hit
NAGGAAANAGCTATGACCTGGAAACCCATGCCATGCACTCAACACTACAA2120.226990449269776No Hit
CAGGAAANAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG2110.22591973960340914No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT2090.22377832027067537No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC2060.22056619127157479No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT2040.21842477193884108No Hit
NAGGAAANAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2010.21521264293974046No Hit
CAGGAAANAGCTATGACCACAGGGGCCTCTTAAAACTGCAAGCCTGACTG1980.21200051394063987No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGACACACCACT1940.20771767527517238No Hit
CAGGAAANAGCTATGACCTCCTGGAACCTGCCTCCGAAGGCCGCAGCTCC1930.2066469656088055No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCCGAGACGGC1920.20557625594243867No Hit
CAGGAAACAGCTATGACCTGGCATCCAGGGCCTATTTGGTTCCTATTGAG1910.2045055462760718No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTAGTCTC1880.20129341727697117No Hit
NAGGAAANAGCTATGACCGTCTCAGGGATCACGTGGCGCAGACCTCGCTC1840.1970105786115037No Hit
CAGGAAANAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT1840.1970105786115037No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA1820.19486915927876997No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCTGCCAGGAGCAGG1770.18951561094693564No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT1730.18523277228146814No Hit
CAGGAAACAGCTATGACCAGCGTCTCATTCCATAAAGGCCACCATGAAAC1710.1830913529487344No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1680.17987922394963382No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGGGACATGGCC1680.17987922394963382No Hit
NAGGAAANAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1680.17987922394963382No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG1670.17880851428326697No Hit
NAGGAAANAGCTATGACCGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA1660.17773780461690009No Hit
CAGGAAANAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC1640.17559638528416635No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCACCCCACCCTGCT1630.17452567561779947No Hit
NAGGAAANAGCTATGACCTCCTGCCCCACCCTGATACAGGTCACTGTGGA1620.17345496595143262No Hit
CAGGAAANAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT1620.17345496595143262No Hit
CAGGAAANAGCTATGACCTCCTGGAACCTGCCTCCGAACGCAGCTCCCCA1610.17238425628506573No Hit
NAGGAAANAGCTATGACCAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1600.17131354661869888No Hit
CAGGAAACAGCTATGACCACCTTTACCCAAATAGCTACCACCAGTGCCTT1570.16810141761959826No Hit
CAGGAAANAGCTATGACCTGCCAAAATTAAGGATGCACACCTGGAGTCAC1550.16595999828686453No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCAGCACAGGGAGT1540.16488928862049768No Hit
CAGGAAANAGCTATGACCTGACTGAAAGGCTTTTCCACGTGCACTGCTGG1540.16488928862049768No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGAAGGGCCACCCCA1540.16488928862049768No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCCGTCCTCGGAC1530.1638185789541308No Hit
NAGGAAANAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1510.16167715962139706No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGCCCCGGGAGCTG1510.16167715962139706No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGTCTGCAGCCTGG1500.16060644995503018No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCTCTGCAGCTAGC1500.16060644995503018No Hit
NAGGAAANAGCTATGACCGCTGCTGACCCAACCCTACCCAGGAAACAGCT1490.15953574028866332No Hit
CAGGAAACAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCCAAGACGGC1490.15953574028866332No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC1470.1573943209559296No Hit
NAGGAAANAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT1460.15632361128956274No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCGGGGATCCTCG1450.15525290162319585No Hit
CAGGAAANAGCTATGACCGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG1420.15204077262409524No Hit
CAGGAAACAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT1410.15097006295772838No Hit
CAGGAAACAGCTATGACCGTGCTCCTTGGGGACGTAGTCAGGGGCCACGC1390.14882864362499465No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACGGAGGTCCAGCC1390.14882864362499465No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAACACGCAGCTCCC1390.14882864362499465No Hit
CAGGAAANAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1390.14882864362499465No Hit
CAGGAAANAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1390.14882864362499465No Hit
CAGGAAANAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1360.14561651462589403No Hit
CAGGAAANAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT1360.14561651462589403No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAACCATGTGCCC1360.14561651462589403No Hit
CAGGAAANAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA1340.1434750952931603No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGACAGGAAACA1310.1402629662940597No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT1300.13919225662769286No Hit
CAGGAAACAGCTATGACCGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC1300.13919225662769286No Hit
CAGGAAACAGCTATGACCCTGGCCTCCTTCAATGCATTTAATGGCCTTAT1300.13919225662769286No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT1290.13812154696132595No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAACAGCAGACACC1280.1370508372949591No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC1270.13598012762859224No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT1270.13598012762859224No Hit
CAGGAAANAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT1260.13490941796222536No Hit
NAGGAAANAGCTATGACCAACACGGCAGTCAGAGGTGCTGCCCAACACCC1260.13490941796222536No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG1250.1338387082958585No Hit
NAGGAAANAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT1250.1338387082958585No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCATGCTGGGTGGGA1230.13169728896312477No Hit
CAGGAAACAGCTATGACCGGCTGGCACAAAGAACAGCACTGACTGTTTAT1200.12848515996402415No Hit
CAGGAAANAGCTATGACCGCATCACTGAGGGAGGATCCTCCCCGGGCAGA1190.12741445029765727No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCCTGGCTTGTC1170.12527303096492357No Hit
CAGGAAACAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG1170.12527303096492357No Hit
NAGGAAANAGCTATGACCAGGTTGCTGGATTCAGTTTGCTGACCAACTTT1150.12313161163218982No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCCCAGGCTGAGGC1150.12313161163218982No Hit
CAGGAAANAGCTATGACCATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC1140.12206090196582296No Hit
CAGGAAANAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT1140.12206090196582296No Hit
CAGGAAANAGCTATGACCGATGCTCCTTCCTTGGCCCCAGGCCCTGGCGT1130.12099019229945608No Hit
CAGGAAACAGCTATGACCGATGTAGCAGTGCTCTGGAGTCCTCCCTTGCT1130.12099019229945608No Hit
CAGGAAANAGCTATGACCGGCGGGCCCTGATATAGAGCCACTGGCCGTCG1110.11884877296672233No Hit
CAGGAAACAGCTATGACCGGGTCCCACCCATGCTGAAATGTGATCCTCAA1100.11777806330035548No Hit
CAGGAAANAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG1090.11670735363398861No Hit
CAGGAAANAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGTGGAGACGGC1080.11563664396762173No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT1080.11563664396762173No Hit
CAGGAAACAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG1070.11456593430125488No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACCTCCAACCCCGT1070.11456593430125488No Hit
NAGGAAANAGCTATGACCACAGGAGACAGGATTGGGGCTGGGTGCTGGTG1070.11456593430125488No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCAGGAAACAGCTA1060.113495224634888No Hit
CAGGAAANAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT1060.113495224634888No Hit
NAGGAAANAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1050.11242451496852113No Hit
NAGGAAANAGCTATGACCACAGGGGCCTCTTAAAACTGCAAGCCTGACTG1030.11028309563578739No Hit
CAGGAAANAGCTATGACCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1010.10814167630305367No Hit
NAGGAAANAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG1010.10814167630305367No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTGCTCTAGGCTGC1000.10707096663668679No Hit
CAGGAAANAGCTATGACCCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT1000.10707096663668679No Hit
NAGGAAANAGCTATGACCTCCTGGAACCTGCCTCCGAACGCAGCTCCCCA1000.10707096663668679No Hit
NAGGAAANAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT990.10600025697031994No Hit
CAGGAAANAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC980.10492954730395306No Hit
NAGGAAANAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT980.10492954730395306No Hit
NAGGAAANAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC970.10385883763758619No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGGGGAGGGTGG970.10385883763758619No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTCACACCTGTAA960.10278812797121933No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGGGGGCCTGGCT950.10171741830485245No Hit
NAGGAAANAGNTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG940.10064670863848559No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)