FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149091
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAANGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG42392.84322997364026No Hit
TGTAAAANGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA41462.7808519628951447No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG39132.6245715703831887No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA36882.4736570282579096No Hit
TGTAAAANGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT26151.7539623451449116No Hit
TGTAAAANGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT23111.5500600304512009No Hit
TGTAAAACGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT22861.5332917479928365No Hit
TGTAAAANGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA20691.387743056254234No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT20331.3635967295141893No Hit
TGTAAAANGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC20031.343474790564152No Hit
TGTAAAANGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC18031.2093285308972372No Hit
TGTAAAANGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG17911.2012797553172223No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA17881.1992675614222186No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC17361.1643895339088208No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG17251.1570114896271406No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG17191.1529871018371332No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC16331.0953042101803596No Hit
TGTAAAANGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC16131.0818895842136682No Hit
TGTAAAANGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG15781.0584139887719581No Hit
TGTAAAANGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT15591.0456700941036012No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC15551.042987168910263No Hit
TGTAAAANGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC15071.0107920665902033No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT14760.9899993963418315No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC13830.927621385596716No Hit
TGTAAAANGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG12630.8471336297965673No Hit
TGTAAAANGACGGCCAGTAGGTCATCCTCCTGCCTCGGCCTCCCAAAGTG12140.814267796178173No Hit
TGTAAAACGACGGCCAGTAGGTCATCCTCCTGCCTCGGCCTCCCAAAGTG10960.7351215029746933No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG10460.7015849380579646No Hit
TGTAAAANGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC9590.6432313151028567No Hit
TGTAAAANGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT9450.6338410769261726No Hit
TGTAAAANGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT9180.615731331871139No Hit
TGTAAAANGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG9150.6137191379761353No Hit
TGTAAAANGACGGCCAGTTCCTGGGCTTCTCTTGAGTACGGAGGAAAGTG9010.6043288997994514No Hit
TGTAAAANGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC8670.5815240356560758No Hit
TGTAAAANGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGCGGTCATA8420.5647557531977114No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGCGGTCATA8360.560731365407704No Hit
TGTAAAANGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC8330.5587191715127002No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC8240.5526825898276891No Hit
TGTAAAANGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG8030.5385972325626631No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC8010.5372557699659939No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT7920.5312191882809828No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT7840.5258533378943062No Hit
TGTAAAACGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG7650.5131094432259492No Hit
TGTAAAACGACGGCCAGTTCCTGGGCTTCTCTTGAGTACGGAGGAAAGTG7630.5117679806292801No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC7320.4909753103809083No Hit
TGTAAAANGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT7310.49030457908257374No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG7030.4715241027292057No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT6850.4594509393591833No Hit
TGTAAAANGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC6350.4259143744424546No Hit
TGTAAAANGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC6140.4118290171774286No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC6120.4104875545807595No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC5850.3923778095257259No Hit
TGTAAAANGACGGCCAGTGGGGAGAGGAAGGCCAGGAGGAGTGGTCATAG5570.3735973331723578No Hit
TGTAAAANGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC5440.36487782629400833No Hit
TGTAAAANGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT5370.3601827072056663No Hit
TGTAAAANGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT5320.35682905071399346No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTCATAGCTGTTTC5310.3561583194156589No Hit
TGTAAAANGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG5310.3561583194156589No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGGAGGAGTGGTCATAG5270.3534753942223206No Hit
TGTAAAANGACGGCCAGTATCCAGAGCCCAGAGCAGGTCATAGCTGTTTC5250.35213393162565143No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC4970.3333534552722834No Hit
TGTAAAANGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA4660.31256078502391155No Hit
TGTAAAANGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG4660.31256078502391155No Hit
TGTAAAANGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC4660.31256078502391155No Hit
TGTAAAANGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA4650.311890053725577No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT4560.30585347204056584No Hit
TGTAAAANGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG4480.30048762165388926No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT4280.28707299568719774No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC4230.2837193391955249No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG4110.27567056361551No Hit
TGTAAAANGACGGCCAGTACCGACTGGAGAGGAAGGCCAGGAGGAGTGGT4020.2696339819304988No Hit
TGTAAAANGACGGCCAGTACCTCATGTCACGTGCGTCCATGTGAAGAGAG3940.2642681315438222No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA3900.26158520635048393No Hit
TGTAAAANGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG3890.2609144750521494No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG3880.2602437437538148No Hit
TGTAAAANGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG3770.25286569947213444No Hit
TGTAAAACGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA3700.24817058038379244No Hit
TGTAAAANGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA3670.24615838648878874No Hit
TGTAAAACGACGGCCAGTTGTCTGGTGGTGGAAGGAGGTCATAGCTGTTT3650.2448169238921196No Hit
TGTAAAACGACGGCCAGTACCGACTGGAGAGGAAGGCCAGGAGGAGTGGT3640.244146192593785No Hit
TGTAAAANGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC3580.24012180480377757No Hit
TGTAAAANGACGGCCAGTTGTCTGGTGGTGGAAGGAGGTCATAGCTGTTT3580.24012180480377757No Hit
TGTAAAANGACGGCCAGTCCAGAGCCCAGCATGGAAGGGTCATAGCTGTT3520.23609741701377013No Hit
TGTAAAANGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG3510.23542668571543557No Hit
TGTAAAANGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCAATCATCTAGG3440.2307315666270935No Hit
TGTAAAANGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC3400.2280486414337552No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC3380.22670717883708608No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG3340.22402425364374778No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG3330.2233535223454132No Hit
TGTAAAANGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGTCATAGCTGTT3310.22201205974874405No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC3310.22201205974874405No Hit
TGTAAAANGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGGG3220.2159754780637329No Hit
TGTAAAACGACGGCCAGTCCCCACACACAAAGCCTGGTCATAGCTGTTTC3180.2132925528703946No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCATGTGAAGAGAG3180.2132925528703946No Hit
TGTAAAANGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC3150.21128035897539088No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG3140.21060962767705632No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA3140.21060962767705632No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGTCATAGCTGTT3120.20926816508038715No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC3120.20926816508038715No Hit
TGTAAAANGACGGCCAGTGCCATCGGCTCCCAAGTTTTATGTCCTAGAGC3070.2059145085887143No Hit
TGTAAAANGACGGCCAGTCCCCACACACAAAGCCTGGTCATAGCTGTTTC3050.2045730459920451No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGGTCATAGCTGTT3040.20390231469371053No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCAATCATCTAGG3020.2025608520970414No Hit
TGTAAAANGACGGCCAGTTCCCACCACCATACCCCAACTTAGTATTTGAA3000.20121938950037224No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG2970.1992071956053685No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTTAGTATTTGAA2930.19652427041203022No Hit
TGTAAAANGACGGCCAGTTGGAGTGTCTAGGTTTGAAAGCTGCTGGTCAT2870.19249988262202278No Hit
TGTAAAACGACGGCCAGTGCCATCGGCTCCCAAGTTTTATGTCCTAGAGC2820.18914622613034993No Hit
TGTAAAANGACGGCCAGTCTTTCCCAACCCTCTGCCCGGCCGGTGCCCAT2770.18579256963867705No Hit
TGTAAAANGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA2730.18310964444533875No Hit
TGTAAAANGACGGCCAGTAAGAGGTGAGCGAGGTCTGCCGGTCATAGCTG2680.17975598795366587No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGGG2560.171707212373651No Hit
TGTAAAACGACGGCCAGTAAGAGGTGAGCGAGGTCTGCCGGTCATAGCTG2510.16835355588197812No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA2500.16768282458364353No Hit
TGTAAAANGACGGCCAGTAGAACAATGCGGCACTGAAGGAAGAGATGATT2470.16567063068863983No Hit
TGTAAAACGACGGCCAGTTGGAGTGTCTAGGTTTGAAAGCTGCTGGTCAT2390.16030478030196324No Hit
TGTAAAACGACGGCCAGTAGAACAATGCGGCACTGAAGGAAGAGATGATT2350.15762185510862495No Hit
TGTAAAANGACGGCCAGTTGTCTGGTGGTGGAAGGAGGAGAGGCTGTCTG2110.14152430394859514No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGCCGGTGCCCAT2100.14085357265026058No Hit
TGTAAAANGACGGCCAGTAGCACAGCTGCCCTTGGATCGACATGGAGAAA2100.14085357265026058No Hit
TGTAAAACGACGGCCAGTTGTCTGGTGGTGGAAGGAGGAGAGGCTGTCTG2060.13817064745692229No Hit
TGTAAAANGACGGCCAGTGGTACGTGGTGGTAGCTATTTGGGTAAAGGTG2010.1348169909652494No Hit
TGTAAAANGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGTGAACGAAG1910.12810967798190367No Hit
TGTAAAANGACGGCCAGTGTGGTAACTGCTGCAGGGCTTAATACATTAGT1880.12609748408689994No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTAGCTATTTGGGTAAAGGTG1870.12542675278856538No Hit
TGTAAAANGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCCT1870.12542675278856538No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCCT1860.1247560214902308No Hit
TGTAAAANGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCAGTGGG1840.12341455889356165No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGTGAACGAAG1800.12073163370022336No Hit
TGTAAAACGACGGCCAGTAGCACAGCTGCCCTTGGATCGACATGGAGAAA1700.1140243207168776No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCAAACAGGAAAA1700.1140243207168776No Hit
TGTAAAANGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCCGTGGG1690.11335358941854304No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGTCATAGCTGTT1610.10798773903186645No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTTAATACATTAGT1580.10597554513686272No Hit
TGTAAAANGACGGCCAGTAGTACTCCCTCCTGCCTGTGGTCATAGCTGTT1580.10597554513686272No Hit
TGTAAAANGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCAAACAGGAAAA1580.10597554513686272No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)