FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73638
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG7511.0198538797903256No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT6900.9370162144544937No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT6760.9180042912626634No Hit
CAGGAAANAGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC5710.7754148673239359No Hit
CAGGAAANAGCTATGANNCCCGTGACAGCTCCTGCAATTACAAAATGGCT4750.6450473940085282No Hit
CAGGAAANAGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA4700.6382574214400174No Hit
CAGGAAANAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAATT4550.6178875037344849No Hit
CAGGAAANAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG4520.6138135201933784No Hit
CAGGAAANAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAACT4350.5907276134604417No Hit
CAGGAAANAGCTATGANNGCAAAACACCTTAACACTCGGCTTCTATTTGC4210.5717156902686114No Hit
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC4150.5635677231863984No Hit
CAGGAAANAGCTATGACCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC3990.5418398109671637No Hit
CAGGAAANAGCTATGANNCATGGTTGGGGAAAAACTCCTCATCTTCATGT3660.49702599201499226No Hit
CAGGAAANAGCTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT3660.49702599201499226No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC3550.48208805236426844No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAATT3450.46850810722724684No Hit
CAGGAAANAGCTATGANNCTGGGTGCTGGTGGCAGACTGGCCGTCGTTTT3430.46579211819984245No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAACT3380.4590021456313317No Hit
CAGGAAANAGCTATGANNCATGGTTGGGGAAAAACTCCTCATTTTCATGT3360.45628615660392735No Hit
CAGGAAANAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA3090.41962030473396883No Hit
CAGGAAANAGCTATGACCCCCGTGACAGCTCCTGCAATTACAAAATGGCT2930.3978923925147342No Hit
CAGGAAANAGCTATGACCCTGGGTGCTGGTGGCAGACTGGCCGTCGTTTT2880.3911024199462234No Hit
CAGGAAANAGCTATGANNAAACTATTAATACCTGCAGCAAAGCCCCAAGC2860.3883864309188191No Hit
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG2830.3843124473777126No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG2820.3829544528640104No Hit
CAGGAAANAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG2800.38023846383660614No Hit
CAGGAAANAGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA2750.37344849126809526No Hit
CAGGAAANAGCTATGANNGTTTTCACTGGCTCCCGGGGCTGTTCACTGGC2700.36665851869958443No Hit
CAGGAAANAGCTATGANCGCATCAGATTCACTTCCACTGTCGTCAGAATT2600.3530785735625628No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG2590.3517205790488606No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC2580.3503625845351585No Hit
CAGGAAACAGCTATGACCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC2560.3476465955077542No Hit
CAGGAAANAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC2510.34085662293924335No Hit
CAGGAAANAGCTATGANNCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC2500.3394986284255412No Hit
CAGGAAANAGCTATGANCGCATCAGATTCACTTCCACTGTCGTCAGAACT2440.33135066134332813No Hit
CAGGAAANAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC2410.3272766778022217No Hit
CAGGAAANAGCTATGANNCTGGCCTCCTTCAATGCATTTAATGGCCTTAT2400.32591868328851953No Hit
CAGGAAANAGCTATGANNAGGACTTGCCCACTCTCCCAGTTACTTGGAAT2290.3109807436377957No Hit
CAGGAAANAGCTATGANCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC2290.3109807436377957No Hit
CAGGAAANAGCTATGACCAAACTATTAATACCTGCAGCAAAGCCCCAAGC2280.30962274912409354No Hit
CAGGAAACAGCTATGACCCTGGGTGCTGGTGGCAGACTGGCCGTCGTTTT2200.2987587930144762No Hit
CAGGAAANAGCTATGACCGTTTTCACTGGCTCCCGGGGCTGTTCACTGGC2190.29740079850077406No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA2170.2946848094733697No Hit
CAGGAAANAGCTATGANNGCATCCTGCTTAGTGCTGCCTCAATCTGGTCC2140.29061082593226323No Hit
CAGGAAANAGCTATGACCCATGGTTGGGGAAAAACTCCTCATCTTCATGT2140.29061082593226323No Hit
CAGGAAANAGCTATGACCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT2110.28653684239115673No Hit
CAGGAAANAGCTATGANNTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC2100.28517884787745457No Hit
CAGGAAANAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2080.28246285885005024No Hit
CAGGAAANAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA2070.2811048643363481No Hit
CAGGAAANAGCTATGANNCAGGGTGACTCCGATCCTGGAGGACAAGGCGG2070.2811048643363481No Hit
CAGGAAANAGCTATGANNCTTGGTGGAGCCATAGGCCGCATGCACGCGCA2070.2811048643363481No Hit
CAGGAAACAGCTATGACCCCCGTGACAGCTCCTGCAATTACAAAATGGCT2040.2770308807952416No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA2030.2756728862815394No Hit
CAGGAAANAGCTATGACCCATGGTTGGGGAAAAACTCCTCATTTTCATGT1980.2688829137130286No Hit
CAGGAAANAGCTATGANNTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC1970.2675249191993264No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC1970.2675249191993264No Hit
CAGGAAANAGCTATGANCCCCGTGACAGCTCCTGCAATTACAAAATGGCT1930.26209294114451775No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG1920.26073494663081564No Hit
CAGGAAANAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1920.26073494663081564No Hit
CAGGAAANAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1890.2566609630897091No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1840.2498709905211983No Hit
CAGGAAANAGCTATGANNTCCCACCACTGACTCCAGGGGAAGGAGGAGGA1810.2457970069800918No Hit
CAGGAAANAGCTATGANNCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA1800.24443901246638963No Hit
CAGGAAANAGCTATGANCACAGTGTCCCCATCAGGCTCCGGGACAGCATC1760.239007034411581No Hit
CAGGAAANAGCTATGANCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1750.2376490398978788No Hit
CAGGAAANAGCTATGANNGTTTTCACTTGCTTCCGGGGCTGTTCACTGGC1730.23493305087047447No Hit
CAGGAAANAGCTATGANNAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC1730.23493305087047447No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG1710.23221706184307017No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGCTGTTCACTGGC1690.22950107281566584No Hit
CAGGAAACAGCTATGACCCATGGTTGGGGAAAAACTCCTCATCTTCATGT1640.22271110024715499No Hit
CAGGAAANAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC1630.22135310573345285No Hit
CAGGAAANAGCTATGANCCTGGGTGCTGGTGGCAGACTGGCCGTCGTTTT1600.21727912219234635No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC1570.21320513865123983No Hit
CAGGAAANAGCTATGACCCTGGCCTCCTTCAATGCATTTAATGGCCTTAT1540.20913115511013336No Hit
CAGGAAANAGCTATGANCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1540.20913115511013336No Hit
CAGGAAANAGCTATGANCGCAAAACACCTTAACACTCGGCTTCTATTTGC1500.20369917705532467No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1500.20369917705532467No Hit
CAGGAAANAGCTATGANCCATGGTTGGGGAAAAACTCCTCATCTTCATGT1480.20098318802792037No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA1480.20098318802792037No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1480.20098318802792037No Hit
CAGGAAANAGCTATGACCAGGACTTGCCCACTCTCCCAGTTACTTGGAAT1460.19826719900051604No Hit
CAGGAAANAGCTATGANCCATGGTTGGGGAAAAACTCCTCATTTTCATGT1440.1955512099731117No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA1440.1955512099731117No Hit
CAGGAAANAGCTATGANNCTGGGTGCCGGGTAGAGTGTATACAGGGCTCA1400.19011923191830307No Hit
CAGGAAACAGCTATGACCCATGGTTGGGGAAAAACTCCTCATTTTCATGT1400.19011923191830307No Hit
CAGGAAANAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC1390.18876123740460088No Hit
CAGGAAANAGCTATGANNCCCTGCGGCTCCTTTCAAGTTAAGTCACCACT1380.1874032428908987No Hit
CAGGAAACAGCTATGACCAAACTATTAATACCTGCAGCAAAGCCCCAAGC1370.18604524837719655No Hit
CAGGAAANAGCTATGANNAAGTTGGTCAGCCCTGGTGACTCATTGGATAT1360.1846872538634944No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA1350.18332925934979222No Hit
CAGGAAANAGCTATGANNGGAGAAGCACAGCTTCGGCTCTGGCCCTGGGG1340.18197126483609005No Hit
CAGGAAANAGCTATGANNGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT1330.1806132703223879No Hit
CAGGAAANAGCTATGANCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1310.17789728129498356No Hit
CAGGAAANAGCTATGACCGTTTTCACTTGCTTCCGGGGCTGTTCACTGGC1310.17789728129498356No Hit
CAGGAAANAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC1300.1765392867812814No Hit
CAGGAAANAGCTATGACCCTTGGTGGAGCCATAGGCCGCATGCACGCGCA1280.1738232977538771No Hit
CAGGAAACAGCTATGACCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1260.17110730872647273No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1250.1697493142127706No Hit
CAGGAAANAGCTATGANCAAACTATTAATACCTGCAGCAAAGCCCCAAGC1250.1697493142127706No Hit
CAGGAAANAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1250.1697493142127706No Hit
CAGGAAANAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC1250.1697493142127706No Hit
CAGGAAANAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG1250.1697493142127706No Hit
CAGGAAANAGCTATGACCGCATCCTGCTTAGTGCTGCCTCAATCTGGTCC1210.16431733615796193No Hit
CAGGAAANAGCTATGANCGTTTTCACTGGCTCCCGGGGCTGTTCACTGGC1210.16431733615796193No Hit
CAGGAAANAGCTATGANNTGCCAAAATTAAGGATGCACACCTGTGACACA1210.16431733615796193No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1190.1616013471305576No Hit
CAGGAAACAGCTATGACCCTGGCCTCCTTCAATGCATTTAATGGCCTTAT1190.1616013471305576No Hit
CAGGAAANAGCTATGACCTCCCACCACTGACTCCAGGGGAAGGAGGAGGA1180.16024335261685543No Hit
CAGGAAANAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG1180.16024335261685543No Hit
CAGGAAANAGCTATGANCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1170.15888535810315327No Hit
CAGGAAANAGCTATGANNGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT1160.1575273635894511No Hit
CAGGAAANAGCTATGANNGCTGCTGACCCAACCCTACCCTGTCCAGCCCC1140.15481137456204677No Hit
CAGGAAANAGCTATGANCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1120.15209538553464244No Hit
CAGGAAANAGCTATGACCAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC1100.1493793965072381No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1080.14666340747983378No Hit
CAGGAAANAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG1060.14394741845242945No Hit
CAGGAAANAGCTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG1050.14258942393872728No Hit
CAGGAAANAGCTATGANNAGCAGCTTTCAAACCTAGACACTGGCAGGTGG1050.14258942393872728No Hit
CAGGAAANAGCTATGANNCTGGCCTCCTTCAATGCATTTAATGGCCTTCT1030.13987343491132295No Hit
CAGGAAANAGCTATGACCCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA1020.1385154403976208No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC1010.13715744588391862No Hit
CAGGAAANAGCTATGACCCTGGGTGCCGGGTAGAGTGTATACAGGGCTCA1010.13715744588391862No Hit
CAGGAAACAGCTATGACCAGGACTTGCCCACTCTCCCAGTTACTTGGAAT1010.13715744588391862No Hit
CAGGAAANAGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT1000.13579945137021648No Hit
CAGGAAACAGCTATGACCGCATCCTGCTTAGTGCTGCCTCAATCTGGTCC990.1344414568565143No Hit
CAGGAAANAGCTATGACCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT990.1344414568565143No Hit
CAGGAAANAGCTATGACCGGAGAAGCACAGCTTCGGCTCTGGCCCTGGGG980.13308346234281213No Hit
CAGGAAANAGCTATGANCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC980.13308346234281213No Hit
CAGGAAANAGCTATGANNGCTACGGGCAATCACTCTCCTACTCCCAACCA960.13036747331540782No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA960.13036747331540782No Hit
CAGGAAANAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA950.12900947880170566No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC940.12765148428800346No Hit
CAGGAAANAGCTATGANCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC910.12357750074689698No Hit
CAGGAAANAGCTATGANNCTGGGTGCCGGGTAGAGTGTTGGGAAAGACTG910.12357750074689698No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC900.12221950623319482No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG880.1195035172057905No Hit
CAGGAAANAGCTATGACCCCCTGCGGCTCCTTTCAAGTTAAGTCACCACT870.11814552269208832No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA860.11678752817838615No Hit
CAGGAAANAGCTATGANCAGGACTTGCCCACTCTCCCAGTTACTTGGAAT860.11678752817838615No Hit
CAGGAAANAGCTATGANCCTGGCCTCCTTCAATGCATTTAATGGCCTTAT860.11678752817838615No Hit
CAGGAAANAGCTATGANNCTGGGTGCCGGGCAGAGGGTTGGGAAAGACTG840.11407153915098184No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG840.11407153915098184No Hit
CAGGAAANAGCTATGANCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA840.11407153915098184No Hit
CAGGAAANAGCTATGANCCTTGGTGGAGCCATAGGCCGCATGCACGCGCA830.11271354463727966No Hit
CAGGAAANAGCTATGANCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC830.11271354463727966No Hit
CAGGAAANAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTGACACA820.11135555012357749No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG820.11135555012357749No Hit
CAGGAAANAGCTATGANCGTTTTCACTTGCTTCCGGGGCTGTTCACTGGC820.11135555012357749No Hit
CAGGAAANAGCTATGANNTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC820.11135555012357749No Hit
CAGGAAANAGCTATGANNGCCCTGGCCACTGTGGTTAATGAAACTTGGTT800.10863956109617318No Hit
CAGGAAANAGCTATGACCAGCAGCTTTCAAACCTAGACACTGGCAGGTGG800.10863956109617318No Hit
CAGGAAANAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC790.107281566582471No Hit
CAGGAAANAGCTATGANCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA770.10456557755506668No Hit
CAGGAAANAGCTATGACCCTGGCCTCCTTCAATGCATTTAATGGCCTTCT750.10184958852766234No Hit
CAGGAAANAGCTATGANCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA750.10184958852766234No Hit
CAGGAAANAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT750.10184958852766234No Hit
CAGGAAANAGCTATGACCGCTACGGGCAATCACTCTCCTACTCCCAACCA740.10049159401396018No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT740.10049159401396018No Hit
CAGGAAACAGCTATGACCTCCCACCACTGACTCCAGGGGAAGGAGGAGGA740.10049159401396018No Hit
CAGGAAANAGCTATGANCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG740.10049159401396018No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)