FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80779
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG6640.821995815744191No Hit
CAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT6490.8034266331596084No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT6090.7539088129340547No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA5930.7341016848438331No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT5640.6982012651803068No Hit
CAGGAAANAGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT5600.6932494831577515No Hit
CAGGAAANAGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA4960.6140209707968655No Hit
CAGGAAANAGCTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC4870.6028794612461159No Hit
CAGGAAANAGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT4580.5669790415825895No Hit
CAGGAAANAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT4290.5310786219190632No Hit
CAGGAAANAGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC4280.5298406764134244No Hit
CAGGAAANAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA4190.5186991668626747No Hit
CAGGAAANAGCTATGACCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT4070.5038438207950086No Hit
CAGGAAANAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG3980.492702311244259No Hit
CAGGAAANAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT3700.4580398370863714No Hit
CAGGAAANAGCTATGANNCCCGTGACAGCTCCTGCAATTACAAAATGGCT3630.4493742185468995No Hit
CAGGAAANAGCTATGANNACAAACGCAAATGGCTTTTAGCATTTCACAGG3570.4419465455130665No Hit
CAGGAAANAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT3520.43575681798487226No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT3500.4332809269735946No Hit
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC3410.422139417422845No Hit
CAGGAAANAGCTATGACCGGGGAGACTGGGCCATACGATAGGCTTAATCC3240.4010943438269847No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGACTGGCCGTCG3080.3812872157367633No Hit
CAGGAAANAGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA3080.3812872157367633No Hit
CAGGAAANAGCTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC3060.3788113247254856No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT3050.37757337921984674No Hit
CAGGAAANAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA3050.37757337921984674No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2920.3614800876465418No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2890.3577662511296253No Hit
CAGGAAANAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT2840.35157652360143105No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2710.3354832320281261No Hit
CAGGAAACAGCTATGACCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2670.33053145000557077No Hit
CAGGAAANAGCTATGANNGCAAAACACCTTAACACTCGGCTTCTATTTGC2590.32062788596046No Hit
CAGGAAANAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2580.31938994045482116No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA2570.31815199494918234No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG2460.3045345943871551No Hit
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG2410.2983448668589609No Hit
CAGGAAANAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC2400.29710692135332206No Hit
CAGGAAANAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG2390.2958689758476832No Hit
CAGGAAANAGCTATGANNCTGGGTGCTGGTGGCAGACTGGCCGTCGTTTT2360.29215513933076664No Hit
CAGGAAANAGCTATGACCCCCGTGACAGCTCCTGCAATTACAAAATGGCT2280.2822515752856559No Hit
CAGGAAANAGCTATGACCCTGTGAGGCACCAGAGCACCGACTGGCCGTCG2260.27977568427437827No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT2240.27729979326310056No Hit
CAGGAAANAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT2240.27729979326310056No Hit
CAGGAAANAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC2200.2723480112405452No Hit
CAGGAAANAGCTATGANCTGGGGATGAGGACAGCAGGGCAGTCACCATCT2180.2698721202292675No Hit
CAGGAAANAGCTATGANCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2160.26739622921798983No Hit
CAGGAAANAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG2110.26120650168979564No Hit
CAGGAAANAGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA2090.25873061067851794No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA2060.2550167741616014No Hit
CAGGAAANAGCTATGANCAGGCACCAACAGTACAAAGCAGATACAAAGCA2050.2537788286559625No Hit
CAGGAAANAGCTATGANNTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG2040.2525408831503237No Hit
CAGGAAANAGCTATGANNGCAGCAAGAGACCCAGACCCACCCGACTGGCC2000.24758910112776836No Hit
CAGGAAANAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC1970.24387526461085185No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1940.24016142809393531No Hit
CAGGAAACAGCTATGACCGGGGAGACTGGGCCATACGATAGGCTTAATCC1940.24016142809393531No Hit
CAGGAAANAGCTATGANNTCAAGCAAGTTCAGGATGGTATAGAAATAGAG1920.23768553708265763No Hit
CAGGAAANAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT1910.2364475915770188No Hit
CAGGAAANAGCTATGACCCTGGGTGCTGGTGGCAGACTGGCCGTCGTTTT1910.2364475915770188No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCAGAGGGAAGGTGACT1910.2364475915770188No Hit
CAGGAAANAGCTATGACCTGGAAACCCATGCCATGCACTCAACACTACAA1860.2302578640488246No Hit
CAGGAAANAGCTATGANCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1820.22530608202626917No Hit
CAGGAAANAGCTATGANNTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC1820.22530608202626917No Hit
CAGGAAANAGCTATGANCGGGGAGACTGGGCCATACGATAGGCTTAATCC1820.22530608202626917No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1800.22283019101499152No Hit
CAGGAAANAGCTATGANNTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC1770.219116354498075No Hit
CAGGAAANAGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1760.21787840899243613No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1730.21416457247551962No Hit
CAGGAAANAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1720.2129266269698808No Hit
CAGGAAANAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC1720.2129266269698808No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC1700.21045073595860309No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTATGTGCA1690.20921279045296426No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT1670.20673689944168658No Hit
CAGGAAANAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1660.20549895393604775No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT1630.20178511741913122No Hit
CAGGAAANAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT1630.20178511741913122No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT1600.1980712809022147No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT1590.19683333539657583No Hit
CAGGAAANAGCTATGANCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC1590.19683333539657583No Hit
CAGGAAANAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1580.195595389890937No Hit
CAGGAAANAGCTATGANCGGGACCAGTCCAATAACCCCTGAGGGGTATAT1560.19311949887965932No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT1550.19188155337402046No Hit
CAGGAAACAGCTATGACCCCCGTGACAGCTCCTGCAATTACAAAATGGCT1550.19188155337402046No Hit
CAGGAAANAGCTATGANNCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA1530.1894056623627428No Hit
CAGGAAANAGCTATGANNCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG1520.18816771685710396No Hit
CAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1490.18445388034018742No Hit
CAGGAAANAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA1480.18321593483454857No Hit
CAGGAAANAGCTATGANNAGGACTTGCCCACTCTCCCAGTTACTTGGAAT1470.18197798932890974No Hit
CAGGAAACAGCTATGACCTGGAAACCCATGCCATGCACTCAACACTACAA1440.1782641528119932No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC1420.17578826180071552No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGACTGGCCGTCG1400.17331237078943787No Hit
CAGGAAANAGCTATGACCGCAGCAAGAGACCCAGACCCACCCGACTGGCC1390.17207442528379902No Hit
CAGGAAANAGCTATGANCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT1390.17207442528379902No Hit
CAGGAAACAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG1360.16836058876688248No Hit
CAGGAAANAGCTATGACCACCAAGACCCCAGCAGCAGAGGGAAGGTGACT1340.1658846977556048No Hit
CAGGAAANAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1330.16464675224996597No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1320.16340880674432712No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTGGCCGTC1320.16340880674432712No Hit
CAGGAAANAGCTATGANNCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC1300.16093291573304944No Hit
CAGGAAANAGCTATGANCTGGAAACCCATGCCATGCACTCAACACTACAA1300.16093291573304944No Hit
CAGGAAANAGCTATGANCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC1270.15721907921613293No Hit
CAGGAAACAGCTATGACCCTGGGTGCTGGTGGCAGACTGGCCGTCGTTTT1270.15721907921613293No Hit
CAGGAAANAGCTATGACCCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA1270.15721907921613293No Hit
CAGGAAANAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG1270.15721907921613293No Hit
CAGGAAANAGCTATGANCCCCGTGACAGCTCCTGCAATTACAAAATGGCT1260.15598113371049407No Hit
CAGGAAANAGCTATGACCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG1250.15474318820485522No Hit
CAGGAAANAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA1230.15226729719357754No Hit
CAGGAAANAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1210.14979140618229986No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA1200.14855346067666103No Hit
CAGGAAANAGCTATGANNAAGTTGGTCAGCCCTGGTGACTCATTGGATAT1180.14607756966538332No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC1170.1448396241597445No Hit
CAGGAAANAGCTATGANCCTGTGAGGCACCAGAGCACCGACTGGCCGTCG1160.14360167865410564No Hit
CAGGAAANAGCTATGANCACAAACGCAAATGGCTTTTAGCATTTCACAGG1150.1423637331484668No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1140.14112578764282796No Hit
CAGGAAANAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC1140.14112578764282796No Hit
CAGGAAANAGCTATGANNGGCAGACAGCCTCTCCTCCTTTCATCACAAAA1110.13741195112591142No Hit
CAGGAAANAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1100.1361740056202726No Hit
CAGGAAANAGCTATGACCAGGACTTGCCCACTCTCCCAGTTACTTGGAAT1100.1361740056202726No Hit
CAGGAAANAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC1100.1361740056202726No Hit
CAGGAAANAGCTATGANCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1080.13369811460899492No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1080.13369811460899492No Hit
CAGGAAANAGCTATGANNGCTGCTGACCCAACCCTACCCTGTCCAGCCCC1070.1324601691033561No Hit
CAGGAAANAGCTATGANCACAGTGTCCCCATCAGGCTCCGGGACAGCATC1070.1324601691033561No Hit
CAGGAAANAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1060.13122222359771724No Hit
CAGGAAANAGCTATGANNCTGGGTGCCGGGTAGAGTGTATACAGGGCTCA1030.1275083870808007No Hit
CAGGAAANAGCTATGANCCTGGGTGCTGGTGGCAGACTGGCCGTCGTTTT1020.12627044157516185No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA1010.12503249606952302No Hit
CAGGAAACAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC1000.12379455056388418No Hit
CAGGAAANAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT980.12131865955260648No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG980.12131865955260648No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC980.12131865955260648No Hit
CAGGAAANAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG980.12131865955260648No Hit
CAGGAAANAGCTATGANCGCAAAACACCTTAACACTCGGCTTCTATTTGC970.12008071404696766No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA940.11636687753005114No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA930.1151289320244123No Hit
CAGGAAANAGCTATGANCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG930.1151289320244123No Hit
CAGGAAACAGCTATGACCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG930.1151289320244123No Hit
CAGGAAANAGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT920.11389098651877344No Hit
CAGGAAANAGCTATGANCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC910.11265304101313459No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA900.11141509550749576No Hit
CAGGAAANAGCTATGACCTTCATACAGAGATACAAAGGCAACTGGCCGTC900.11141509550749576No Hit
CAGGAAACAGCTATGACCGCAGCAAGAGACCCAGACCCACCCGACTGGCC900.11141509550749576No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG900.11141509550749576No Hit
CAGGAAANAGCTATGANNGGCTGGCACAAAGAACAGCACTGACTGTTTAT880.10893920449621806No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG880.10893920449621806No Hit
CAGGAAANAGCTATGANCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA880.10893920449621806No Hit
CAGGAAANAGCTATGANNCTGGGTGCCGGGTAGAGTGTTGGGAAAGACTG880.10893920449621806No Hit
CAGGAAANAGCTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT870.10770125899057924No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG860.1064633134849404No Hit
CAGGAAANAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT860.1064633134849404No Hit
CAGGAAANAGCTATGACCCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG850.10522536797930154No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA840.10398742247366272No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC840.10398742247366272No Hit
CAGGAAANAGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT830.10274947696802388No Hit
CAGGAAANAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT830.10274947696802388No Hit
CAGGAAANAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG830.10274947696802388No Hit
CAGGAAANAGCTATGANCGCAGCAAGAGACCCAGACCCACCCGACTGGCC820.10151153146238502No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)