FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003245817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194014
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG86244.445040048656282No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA83974.32803818281155No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT68233.516756522725164No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG52452.7034131557516465No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA51922.6760955394971497No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG47082.426629006154195No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG46822.4132279113878377No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT39072.0137722020060407No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG32331.6663745915243229No Hit
TGTAAAACGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT32191.6591586174193615No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC31371.6168936262331586No Hit
TGTAAAACGACGGCCAGTTCCCAAAGGCTGGTGGAGGAGATGCTGAAGAT31001.5978228375271888No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA30541.5741132083251723No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC26401.3607265455070252No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG23911.2323852917830673No Hit
TGTAAAACGACGGCCAGTTCTTTGGCTCTACCTAAAAGTTGGTCATAGCT23731.223107610790974No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT21821.1246611069304278No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC20811.0726030080303484No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA18360.9463234611935221No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG17070.8798334140835198No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTGGTGGTCC11540.5948024369375405No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTAGGGTTGGGTCA11450.590163596441494No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG10970.5654231137959116No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC10870.560268846578082No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT10270.5293432432711042No Hit
TGTAAAACGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT10060.5185192821136618No Hit
TGTAAAACGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC10050.5180038553918789No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA9740.502025627016607No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC9690.4994484934076922No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA9670.49841763996412625No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC9520.49068623913738185No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC7570.39017802838970383No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCCCCCATGAACGG7270.3747152267362149No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA6870.35409815786489635No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC6700.34533590359458594No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT6380.32884224849753113No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA6250.3221417011143526No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGCTGCCTTTGGTC6190.31904914078365476No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC6120.31544115373117404No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC6050.31183316667869326No Hit
TGTAAAACGACGGCCAGTCAGTTCCCAGGGACTCTGCTCTATGAGAGAGA5990.30874060634799555No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAACTCCAATGCAA5830.3004937787994681No Hit
TGTAAAACGACGGCCAGTAGGTGGCAAAGCTCAATCAGGAGCCCTACCAA5760.2968857917469873No Hit
TGTAAAACGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA5510.28400012370241323No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGGAGGATCCTCC5480.2824538435370643No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA5440.28039213664993246No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG5310.27369158926675397No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG5270.27162988237962205No Hit
TGTAAAACGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG5040.2597750677786139No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGTGTAAAACGACG4960.2556516540043502No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGTGACC4310.22214891708845752No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGGGAGGATCCTC4170.21493294298349602No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGTCTTCTGCGTA4030.20771696887853455No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAGCC3970.20462440854783676No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAGGAGAGTGA3650.18813075345078192No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGGGGCCATCATCC3550.18297648623295226No Hit
TGTAAAACGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT3460.17833764573690558No Hit
TGTAAAACGACGGCCAGTCTGGGTTGGGGTGTCTGCTGCTTGTGCAGGCG3360.17318337851907595No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGTCATAGCTGTT3350.17266795179729297No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGTAACC3270.16854453802302927No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGCATACGTTTT3180.16390569752698259No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCTCAACAAGGAGACGATG3170.1633902708051996No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG3150.1623594173616337No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGTGTAAAACGACG3150.1623594173616337No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGACCAGACG3020.15565886997845518No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC2930.15102002948240847No Hit
TGTAAAACGACGGCTAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG2920.15050460276062552No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG2690.13864978815961734No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGAGGGAGGATCCT2560.13194924077643883No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGTGAACGAAG2460.1267949735586092No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGCA2440.12576412011504323No Hit
TGTAAAACGACGGCCAGTCTGGGTTGGGGTGTCTGCTGAGCCCTTCTTGT2310.11906357273186471No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAACACCTCCACGCAGGA2250.11597101240116692No Hit
TGNAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAATAAGGAGACGATG2230.114940158957601No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGTAAAACGACGGC2200.1133938787922521No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGCGCCTG2190.11287845207046915No Hit
TGTAAAACGACGGCTAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA2190.11287845207046915No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGTGCCTG2170.11184759862690322No Hit
TGAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATGA2140.11030131846155433No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGAAACC2090.1077241848526395No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTAGCACACAGCCA2090.1077241848526395No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGTGGTCTTGCTCC2040.10514705124372468No Hit
TGNAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAATCTCCACGCAGGA2040.10514705124372468No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGA1990.10256991763480985No Hit
TGTAAAACGACGGCTAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG1990.10256991763480985No Hit
TGAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGAG1990.10256991763480985No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)