FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118551
Sequences flagged as poor quality0
Sequence length300
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA25192.124823915445673No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAGTGCCAAGG22191.8717682685089116No Hit
TGTAAAACGACGGCCAGTAAGAGGTGAGCGAGGTCTGCGCCACGTGATCC18741.580754274531636No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGACGGGTGGCC18621.5706320486541656No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGCCCCAATCCTGTCTC18551.564727416892308No Hit
TGTAAAACGACGGCCAGTCCACCTGCTTCGCACCATCTCCATCTCCATTT16341.3783097569822271No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTGTAAAACGACGG15761.3293856652411198No Hit
TGTAAAACGACGGCCAGTACTAAACTGTAGTGAACAGAGACATGCCCAGG15571.3133588076017917No Hit
TGTAAAACGACGGCCAGTACAAAAGTTCTCCAAGTCCCTAGATTAGCTAG15201.2821486111462577No Hit
TGTAAAACGACGGCCAGTTGTCTGGTGGTGGAAGGAGGTGCACGCCACCA15091.2728699040919098No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC13391.1294717041610784No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA12961.0932003947668092No Hit
TGTAAAACGACGGCCAGTTGGAGTGCCTGGGTTAGAACCCAGGCATTCAA12141.0240318512707611No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTAGGGTTGGGTCA11790.9945086924614722No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCGTGACCGAGAGCT10810.9118438477954636No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGCACGCAGGCT10300.8688243878162142No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATCACATTTCAGCA10210.8612327184081112No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC10160.8570151242924986No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGTGCTGCTGAATG10160.8570151242924986No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCGAGCAGGTGGC10070.8494234548843957No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTGGGGTGCT9870.8325530784219449No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCAGGGCTTGAACCC9680.8165262207826167No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG9370.790377137265818No Hit
TGTAAAACGACGGCCAGTCCCAGCACAACTTCCCCCATGTAAAACGACGG9240.7794113925652251No Hit
TGTAAAACGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTCTGGCATGG9100.7676021290415096No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC9000.7591669408102842No Hit
TGTAAAACGACGGCCAGTGCCAATCCTGAGGCCATCAACACAAGGACTGG8860.7473576772865687No Hit
TGTAAAACGACGGCCAGTCAGCCAGTAGCAACAGCAGCTACTGATGTAAG8800.7422965643478334No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT8440.711929886715422No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC8390.7077122925998093No Hit
TGTAAAACGACGGCCAGTACATGCGTGGCCCTGTCTACTCATCCCCCTCT8200.6916854349604812No Hit
TGTAAAACGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG7940.6697539455592951No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA7830.6604752385049473No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCAGGCCCCAAGGA7800.6579446820355797No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC7760.6545706067430894No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGACAGGGTCAGT7600.6410743055731288No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGAAGCCCAGTCAGG7320.6174557785256978No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC6960.5870891008932865No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCAGGTGACTCCAG6870.5794974314851836No Hit
TGTAAAACGACGGCCAGTAAGGGTGGGTCATGGGGTGACACCGTAACTGT6830.5761233561926935No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATCGCATTTCAGCA6440.5432261220909145No Hit
TGTAAAACGACGGCCAGTTTGCTGCTGGGCTTCCTCTGCACTTGAAAGGA6110.5153900009278708No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCCGGCCCCAAGGA5920.4993631432885425No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA5860.49430203034980724No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCCGAGTAGCTGAG5810.4900844362341946No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCACCACGTGCAGGA5660.47743165388735653No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGCCTTCGGAGGC5520.46562239036364095No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGATCCCCGGTG5480.46224831507115083No Hit
TGTAAAACGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT5480.46224831507115083No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGACGGCACTGGTG5480.46224831507115083No Hit
TGTAAAACGACGGCCAGTCCCAGCACAACTTCCCCCATGGCGTGGTGTCC5410.45634368330929304No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGTCCAACAATGGCCTG5300.4470649762549452No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCGCAGCCCTCCTAC5270.44453441978557756No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC5180.4369427503774747No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGTTCGGAGGCAGG5150.4344121939081071No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAGGAGGACCC5000.421759411561269No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGTGAACGAAG4950.4175418174456563No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCAAGGGT4940.4166982986225338No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGGGAGGATCCTC4930.4158547797994112No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCCACGCTGGGAAT4890.4124807045069211No Hit
TGTAAAACGACGGCCAGTTGAGAAGCTGAGGCTTAAAGAGGGATCCCTTG4870.410793666860676No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA4860.4099501480375535No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC4660.39307977157510265No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGGTCTGTGCGG4610.38886217745949No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGTGTAAAACTACG4460.37620939511265195No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCAGTTTCCTGCC4450.3753658762895294No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT4390.37030476335079415No Hit
TGTAAAACGACGGCCAGTTGCCCATCAGCAATCCTGCCTTCGACATACAA4300.3627130939426913No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG4240.35765198100395607No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCCCCAGTAGCTGG4210.35512142453458845No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC4130.34837327394960815No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGGGCGGCGGCTCCT4040.34078160454150536No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCGTGACAAGGCT4020.3390945668952603No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG3840.3239112280790546No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCAGAAATCAGGCTG3820.3222241904328095No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCAGGGCCATGTG3710.31294548337846156No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCTCCCCAGTCACC3670.3095714080859714No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGACATTCCTGCAA3500.2952315880928883No Hit
TGTAAAACGACGGCCAGTGCCCTGAACCGGTGCCAAATAATGACACTGTG3390.2859528810385404No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCCAAAGTGCTGGG3310.2792047304535601No Hit
TGTAAAACGACGGCCAGTCCCAGCACAACTTCCCCCATGGAACAGAAACG3150.26570842928359945No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTACCCAGTGGTGTG3140.26486491046047694No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCAGGAAATCAGGCT3130.2640213916373544No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCCGCCCCAAGGAT3030.25558620340612903No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAGGAGAGTGA3030.25558620340612903No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTTGGGAAGGGA3020.2547426845830065No Hit
TGTAAAACGACGGCCAGTCTCTCACCCACCTCCACCCACCAACAGGCCCC2970.2505250904673938No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC2920.24630749635178112No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTGACCCTTCCAGGA2700.22775008224308527No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGGGCCGGCCCGT2690.2269065634199627No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAATTAGTAGT2620.22100193165810497No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCATGACAAGGCT2610.22015841283498241No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGCACGGCACTGGT2590.21847137518873735No Hit
TGTAAAACGACGGCCAGTGCCATCGGCTCCCAAGTTTTACATCCTGGAGA2490.21003618695751197No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTTGCGGCCCCTG2450.2066621116650218No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCAACCGT2440.2058185928418993No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCTGGCCCAAGTCA2420.20413155519565418No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGTGTTCGGAGGCA2380.20075747990316406No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCTGATCAGTCACC2370.1999139610800415No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTACAGGAACCATGG2370.1999139610800415No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCTACTTCAATGC2340.19738340461067388No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCACCTTGGCCTCC2330.19653988578755133No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGGGCAGGGCAGCGT2330.19653988578755133No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTGCGGCCCCTGA2320.19569636696442883No Hit
TGTAAAACGACGGCCAGTAAGGGTGGGTCATGGGGTGAGGGTGGGTCGCG2290.1931658104950612No Hit
TGTAAAACGACGGCCAGTCTCTCACCCACCTCCACCCAGTCACCCCTGCA2280.19232229167193865No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGTGTGTGTTCGTGC2240.1889482163794485No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGCTGAGGGAGGAT2220.18726117873320344No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC2200.18557414108695836No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT2200.18557414108695836No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGCCAGCCCCCCAC2180.18388710344071327No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGCCAGTTAACCA2140.18051302814822312No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCAGGAAACAACT2120.17882599050197803No Hit
TGTAAAACGACGGCCAGTCCAGGCATCTCCCAGCTGCGGTCATAGCTGTT2120.17882599050197803No Hit
TGTAAAACGACGGCCAGTGCCCAAGGTTGGTGAGATGGGGCCAGGTCATA2070.17460839638636536No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGGTCCCCGGATGCC2060.17376487756324283No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCACTAGGAGAG2020.17039080227075268No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC2010.16954728344763015No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGCTGCCGTGTCC1980.16701672697826253No Hit
TGTAAAACGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT1980.16701672697826253No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGGCCCTCGTTCCC1900.1602685763932822No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTAGGAACCATGGCT1890.15942505757015968No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATCACGGTGGT1870.1577380199239146No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGCAGGGTGTGGAT1860.15689450110079206No Hit
TGTAAAACGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC1830.15436394463142444No Hit
TGTAAAACGACGGCCAGTTGTCTGGTGGTGGAAGGAGGAGGTTGCAGTGA1770.14930283169268924No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGTACGGCACTGGT1760.1484593128695667No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGGTGGCCCTTC1690.1425546811077089No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGTGTAAAACGACG1670.14086764346146385No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCTCCTCAGTCACC1650.13918060581521877No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGTGTGTTCGTGCTT1620.13665004934585115No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGAGGATCCTCCC1550.13074541758399338No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCTATGGGTGGGG1530.12905837993774832No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGACCCGTGTCCG1530.12905837993774832No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGCCCAGCACGACG1530.12905837993774832No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTGCTCCACA1510.12737134229150324No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCATCACCAGG1500.1265278234683807No Hit
TGTAAAACGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTTTCCTGTCA1480.12484078582213562No Hit
TGTAAAACGACGGCCAGTCCTGCCGCCTGGTCTCACGTCATGCTGTTCTT1480.12484078582213562No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC1470.12399726699901309No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCGGGGACCGAGAGC1430.12062319170652294No Hit
TGTAAAACGACGGCCAGTTCATAGCTGTGAAGAGGGATACCAAGGAAAGT1410.11893615406027785No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCCGAGTAGCTGGG1370.11556207876778771No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGCGTGTGTTCGTGC1360.11471855994466516No Hit
TGTAAAACGACGGCCAGTTTGCTGCTGGGCTTCCTCTGGGCTGCTCTGTC1330.11218800347529755No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCTGGAGGGCGGCCA1330.11218800347529755No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCACCTCGGCCTAC1330.11218800347529755No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGCTGGGGCCGGA1300.10965744700592993No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG1270.10712689053656232No Hit
TGTAAAACGACGGCCAGTAGCCTCTGGACACCCAGGACACCGCCTACCCC1270.10712689053656232No Hit
TGTAAAACGACGGCCAGTTGCCCATCAGCAATCCTGCCCTGTCTGGCATT1260.1062833717134398No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCTTTCTAACAGG1250.10543985289031725No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA1240.10459633406719471No Hit
TGTAAAACGACGGCCAGTCCTGCCGCCTGGTCTCACCTCGCCATGGTTCG1240.10459633406719471No Hit
TGTAAAACGACGGCCAGTTGCCCATCAGCAATCCTGCCACAAGGACACAG1240.10459633406719471No Hit
TGTAAAACGACGGCCAGTCCCAGCACAACTTCCCCCATTGTAAAACGACG1230.10375281524407218No Hit
TGTAAAACGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCAGGTGTA1210.10206577759782709No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGAGGCTTTTTGTTCCA1190.10037873995158203No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)