FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118551
Sequences flagged as poor quality0
Sequence length300
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGCACTCCTTC23291.9645553390523909No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCCGTCCTCGGAC18781.584128349824126No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCTGGGTGCTGGTG18711.5782237180622685No Hit
CAGGAAACAGCTATGACCGTCTCAGGGATCACGTGGCGCAGACCTCGCTC18571.566414454538553No Hit
CAGGAAACAGCTATGACCTCCACAAGGCCCTGGGACAAGGAAAATGGAGA16631.4027718028527807No Hit
CAGGAAACAGCTATGACCTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT15991.3487865981729381No Hit
CAGGAAACAGCTATGACCGGGCATGGTGTGGGACTAGCTAATCTAGGGAC15491.3066106570168112No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC15261.287209724084993No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAACT13161.11007077122926No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC13141.108383733583015No Hit
CAGGAAACAGCTATGACCTTGGACAGCACCTTGAATGCCTGGGTTCTAAC12861.0847652065355837No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAATT12611.0636772359575204No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCTCTGCAGCTAGC12541.0577726041956625No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGCAGGAAACAGCT12291.0366846336175992No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCACGGGCATCCTG11540.9734207218834088No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGACTGGCCGTCGTT10760.9076262536798508No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC10750.9067827348567283No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACGGAGATCCAGCC10520.8873818019249099No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCAGAGCCACC10500.8856947642786649No Hit
CAGGAAACAGCTATGACCGGGTCCCACCCATGCTGAAATGTGATCCTCAA10440.8806336513399297No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCACCCCACCCTGCT10140.8553280866462535No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA10080.8502669737075184No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG9590.808934551374514No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC9570.8072475137282688No Hit
CAGGAAACAGCTATGACCTCCAGCCAGCCAGTCCTTGTGTTGATGGCCTC9240.7794113925652251No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT9220.77772435491898No Hit
CAGGAAACAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT9020.7608539784565292No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT8820.7439836019940785No Hit
CAGGAAACAGCTATGACCTGCTGGGAGAGGGGGATGAGTAGACAGGGCCA8720.7355484137628531No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA8650.7296437820009953No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA8400.7085558114229319No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA8310.700964142014829No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT8140.686624322021746No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCAAGCCTGACTG7810.6587882008587022No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACCCTGTCTCTCTAGC7610.6419178243962513No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGGAGTCAC7490.6317955985187809No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT7370.6216733726413104No Hit
CAGGAAACAGCTATGACCAGGGCAAGTGTTCGCGTTGTGACAGTTACGGT7100.5988983644170021No Hit
CAGGAAACAGCTATGACCCTTGGTGGAGCCATAGGCCGTCGTTTTACATG7030.5929937326551442No Hit
CAGGAAACAGCTATGACCGGGTCCCACCCATGCTGAAATGCGATCCTCAA6730.567688167961468No Hit
CAGGAAACAGCTATGACCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT6680.5634705738458554No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG6520.5499742726758947No Hit
CAGGAAACAGCTATGACCCCCTGCGGCTCCTTTCAAGTGCAGAGGAAGCC6270.5288863020978313No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCCGGAGAGCCTGT6240.5263557456284638No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTAGTCTC6190.522138151512851No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT5910.49851962446542No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCATCCTGCACGTGG5880.49598906799605236No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAGGCCGCAGCTCC5570.4698399844792537No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGACACTGCAGCCTGGG5440.45887423977866065No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCGGGGATCCTCG5430.45803072095553815No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCCATGCTCACCT5390.45465664566304803No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG5380.4538131268399254No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA5200.4386297880237197No Hit
CAGGAAACAGCTATGACCCCGTAGCCACGCCCCTATTGTCTGACCACGCT5200.4386297880237197No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGGTCCTCCTC5110.4310381186156169No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCCCCGGGCAGA5110.4310381186156169No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAACGCAGCTCCCCA5060.42682052450000424No Hit
CAGGAAACAGCTATGACCCATGGTTGGGGAAAAACTCCTCATCTTCATGT4840.40826311039130836No Hit
CAGGAAACAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT4830.40741959156818586No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA4770.4023584786294506No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTAGTTCC4720.3981408845138379No Hit
CAGGAAACAGCTATGACCTCCCACCACTGACTCCAGGGGAAGGAGGAGGA4590.3871751398132449No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA4580.3863316209901224No Hit
CAGGAAACAGCTATGACCGTGCTCCTTGGGGACGTAGTTTTACACAGAAC4520.3812705080513872No Hit
CAGGAAACAGCTATGACCGGAAATAAGTACCTTCTGTGTCCTTGTATGTC4500.3795834704051421No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGATTCTCAGATTCC4440.3745223574664069No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGAAAAGTTTCAGG4410.37199180099703927No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCACAGACCCCACA4380.36946124452767165No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT4230.35680846218083356No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA4000.3374075292490152No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA3990.3365640104258927No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAGGCACTACGT3950.3331899351334025No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGCGGAAGAGGAGCC3860.32559826572529965No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCTGGGTGTCTGC3780.31885011514031936No Hit
CAGGAAACAGCTATGACCTCCAGTGGTGGGGCTCAACACACACTGGGGCC3780.31885011514031936No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGGGGAGGGTGG3650.30788437043972633No Hit
CAGGAAACAGCTATGACCCATGGTTGGGGAAAAACTCCTCATTTTCATGT3620.30535381397035877No Hit
CAGGAAACAGCTATGACCGCATCCTGCTTAGTGCTGCCCACAGTGTCATT3620.30535381397035877No Hit
CAGGAAACAGCTATGACCTGCTTTGGGGCCATGGAGTTGAGCTGTTGAGA3610.3045102951472362No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT3530.2977621445622559No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGACACACCACT3390.2859528810385404No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTGGCATGAAGGA3370.2842658433922953No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA3360.28342232456917277No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT3340.2817352869229277No Hit
CAGGAAACAGCTATGACCCTTGGTGGAGCCATAGGCCGTTTCTGTTCCAT3330.28089176809980515No Hit
CAGGAAACAGCTATGACCCACCGCTGTCCCTTCCCAAGGACTATGCTGAA3110.2623343539911093No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG3080.2598037975217417No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGTCTGGGTCCT3060.25811675987549665No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCCTGGGTGTCTG3010.25389916575988397No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG3000.2530556469367614No Hit
CAGGAAACAGCTATGACCCTGGTCCTCATCCTTGGGGCGGAGAGCCTGTG2970.2505250904673938No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCATA2940.24799453399802618No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTTATCCC2920.24630749635178112No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGATACTACTA2750.23196767635869794No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCAACAGGGACGCA2730.23028063871245288No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGGCCGGCCCCTCA2680.2260630445968402No Hit
CAGGAAACAGCTATGACCCCTGACCCCTTCTTCTCCAGGATGTAAAACTT2660.2243760069505951No Hit
CAGGAAACAGCTATGACCCTTGGTGGAGCCATAGGCCGCATGCACGCGCA2650.2235324881274726No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTGCCATGCTCACC2590.21847137518873735No Hit
CAGGAAACAGCTATGACCCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA2590.21847137518873735No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGTTGAGCTCCTCA2570.21678433754249224No Hit
CAGGAAACAGCTATGACCACCCAGTTAGAGCCATGGTTCCTGTAGGCTAG2520.2125667434268796No Hit
CAGGAAACAGCTATGACCGGCTGGCACAAAGAACAGCACTGACTGTTTAT2480.20919266813438941No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCACAGGGACGCAG2420.20413155519565418No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGACGCTGCCCTGC2400.20244451754940912No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGATCAGGGTGG2360.19907044225691894No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTTGGGCCAGGGT2340.19738340461067388No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGACAGGAAACA2310.19485284814130627No Hit
CAGGAAACAGCTATGACCGTGCTCCTTGGGGACGTAGTTGTTTCCTGCAG2290.1931658104950612No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAACACACACACAC2280.19232229167193865No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCCCTCAGCGGG2270.19147877284881612No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAACACGCAGCTCCC2260.1906352540256936No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGCAGGGGCATCCGG2240.1889482163794485No Hit
CAGGAAACAGCTATGACCAAAGGCAGCAGGGTCGATGGTGGGGGGCTGGC2210.18641765991008089No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAACTGGCCAGCCTG2190.18473062226383583No Hit
CAGGAAACAGCTATGACCGTGCTCCTTGGGGACGTAGTCAGGGGCCACGC2170.18304358461759074No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTAGTCCC2160.1822000657944682No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTAGTGGCATGAA2130.1796695093251006No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA2080.17545191520948789No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAACTGGCCGTCGTT2040.17207783991699777No Hit
CAGGAAACAGCTATGACCACCCAGTTAGAGCCATGGTTCCTAGGCTAGCA2020.17039080227075268No Hit
CAGGAAACAGCTATGACCTGGCCCCAGTGTTGAGAGGTCAGGAAAACAGC1980.16701672697826253No Hit
CAGGAAACAGCTATGACCAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC1980.16701672697826253No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGCAGCCTCAGCCA1970.16617320815513997No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGAACGAGGGCCAGT1960.16532968933201744No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC1910.16111209521640474No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGCGATCTCGTCTC1860.15689450110079206No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTACCATGCTCACC1840.15520746345454697No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGGATCCACACCCT1820.1535204258083019No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGTAATCCCAGC1790.1509898693389343No Hit
CAGGAAACAGCTATGACCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC1780.15014635051581177No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACCACCGTGATCTCTC1770.14930283169268924No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGAGGAGGGTGG1710.144241718753954No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGATGCAGTCAAGAG1680.14171116228458638No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCAGGATAAGATGTG1640.13833708699209624No Hit
CAGGAAACAGCTATGACCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG1630.13749356816897368No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGAAGGGCCACCCCA1630.13749356816897368No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGGTCCTCAGCCAC1610.13580653052272862No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAACACACACACGC1580.133275974053361No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCAGGAAACAGCT1570.13243245523023847No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTCACACCTGTAA1570.13243245523023847No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCGTGCTGGGCCA1550.13074541758399338No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCCGGGCAGAGG1530.12905837993774832No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCGGGCATCCT1510.12737134229150324No Hit
CAGGAAACAGCTATGACCAAACTATTAATACCTGCAGCAAAGCCCCAAGC1430.12062319170652294No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCAGCCACCACCACGTACCACT1430.12062319170652294No Hit
CAGGAAACAGCTATGACCGGCTGGCACAAAGAACAGCATGACGTGAGACC1380.11640559759091024No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGCATCAGTGCTTC1370.11556207876778771No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAACACACGCACAC1360.11471855994466516No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT1350.11387504112154263No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT1340.1130315222984201No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGTAATCCCACA1330.11218800347529755No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGGCCCCAGCCACAG1320.11134448465217502No Hit
CAGGAAACAGCTATGACCACCACACCTGGCCTGAGGATGGGGATAGCACC1280.10797040935968487No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGTATAAAGTTGGA1270.10712689053656232No Hit
CAGGAAACAGCTATGACCGGAAATAAGTACCTTCTGTGTCCTTGTAATGC1260.1062833717134398No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTTGGCATGAAGG1240.10459633406719471No Hit
CAGGAAACAGCTATGACCGGAAATAAGTACCTTCTGTGTCCTTGTGGCAG1230.10375281524407218No Hit
CAGGAAACAGCTATGACCGTCAACAGCATACCTGTACACCTGAGTGTTCC1220.10290929642094965No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT1190.10037873995158203No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC1190.10037873995158203No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGGCTGCTGCGAGT1190.10037873995158203No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)