FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100916
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT17471.731142732569662No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT17171.7014150382496331No Hit
CAGGAAANAGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA13271.3149550120892624No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC11521.141543461889096No Hit
CAGGAAANAGCTATGANNCCAGCAGAGCTCTTCTTACAGAGGCAGGAAAC10681.0583059177930159No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC10241.014705299456974No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG9680.9592136033929208No Hit
CAGGAAANAGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT8380.8303935946727972No Hit
CAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT7970.7897657457687581No Hit
CAGGAAANAGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC7960.7887748226247573No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT7300.7233738951206945No Hit
CAGGAAANAGCTATGANNCATGGTTGGGGAAAAACTCCTCATCTTCATGT7240.7174283562566888No Hit
CAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT6600.6540092750406279No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT6470.6411272741686155No Hit
CAGGAAANAGCTATGANNAGCCTCTCCCCTCGTTCCAGCAACTCAGTGAG6470.6411272741686155No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT6340.6282452732966031No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT6320.6262634270086012No Hit
CAGGAAANAGCTATGANNAGGTTGCTGGATTCAGTTTGCTCAGGAAACAG6210.6153632724245908No Hit
CAGGAAANAGCTATGANNCATGGTTGGGGAAAAACTCCTCATTTTCATGT5700.5648261920805422No Hit
CAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT5660.5608624995045384No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC5350.530143882040509No Hit
CAGGAAANAGCTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT5250.5202346506004994No Hit
CAGGAAANNGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA4850.4805977248404614No Hit
CAGGAAANAGCTATGANNCAGGGTGACTCCGATCCTGGAGGACAAGGCGG4760.4716794165444528No Hit
CAGGAAANNGCTATGANNCCAGCAGAGCTCTTCTTACAGAGGCAGGAAAC4330.4290697213524119No Hit
CAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC4260.4221332593444052No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG4230.4191604899124024No Hit
CAGGAAANAGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG4140.41024218161639386No Hit
CAGGAAANAGCTATGANNGGGACCAGTCCAATAACCCCTGACAGGAAACA4090.40528756589638903No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT3890.38546910301637005No Hit
CAGGAAANAGCTATGANNCCAGCACAGTTTGTGGGCCAGGACTGGCCGTC3820.3785326410083634No Hit
CAGGAAANNGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC3620.3587141781283444No Hit
CAGGAAANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG3560.35276863926433866No Hit
CAGGAAANAGCTATGANNCTTGGTGGAGCCATAGGCCGCATGCACGCGCA3480.344841254112331No Hit
CAGGAAANAGCTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC3420.3388957152483253No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT3380.3349320226723215No Hit
CAGGAAANAGCTATGANNCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA3330.32997740695231675No Hit
CAGGAAANAGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA3220.3190772523683063No Hit
CAGGAAANNGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT3150.3121407903602997No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA3100.3071861746402949No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA3040.3012406357762892No Hit
CAGGAAANNGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC2780.27547663403226447No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA2760.27349478774426256No Hit
CAGGAAANAGCTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG2750.2725038646002616No Hit
CAGGAAANAGCTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT2740.27151294145626065No Hit
CAGGAAANNGCTATGANNAGCCTCTCCCCTCGTTCCAGCAACTCAGTGAG2410.23881247770422925No Hit
CAGGAAANNGCTATGANNAGGTTGCTGGATTCAGTTTGCTCAGGAAACAG2410.23881247770422925No Hit
CAGGAAANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT2410.23881247770422925No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2410.23881247770422925No Hit
CAGGAAANNGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT2400.2378215545602283No Hit
CAGGAAANAGCTATGANNGGCTGGCACAAAGAACAGCACTGACTGTTTAT2370.23484878512822546No Hit
CAGGAAANAGCTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC2370.23484878512822546No Hit
CAGGAAANNGCTATGANNCATGGTTGGGGAAAAACTCCTCATCTTCATGT2330.23088509255222164No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG2280.2259304768322169No Hit
CAGGAAANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT2200.2180030916802093No Hit
CAGGAAANNGCTATGANNCATGGTTGGGGAAAAACTCCTCATTTTCATGT2100.20809386024019977No Hit
CAGGAAANAGCTATGANNCCCAAACCTGCGGAGGCACGGGGATGGCAGTG2060.20413016766419595No Hit
CAGGAAANAGCTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA2050.20313924452019502No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA2050.20313924452019502No Hit
CAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2010.19917555194419123No Hit
CAGGAAANNGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT1960.19422093622418646No Hit
CAGGAAANNGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC1870.1853026279281779No Hit
CAGGAAANAGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT1870.1853026279281779No Hit
NAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1770.17539339648816837No Hit
CAGGAAANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1750.17341155020016646No Hit
CAGGAAANNGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG1720.1704387807681636No Hit
NAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1720.1704387807681636No Hit
CAGGAAANAGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT1700.16845693448016172No Hit
NAGGANANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1700.16845693448016172No Hit
CAGGAAANNGCTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1670.16548416504815888No Hit
NAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1610.15953862618415315No Hit
CAGGAAANNGCTATGANNCCAGCACAGTTTGTGGGCCAGGACTGGCCGTC1590.15755677989615124No Hit
CAGGAAANAGCTATGANNAAACTATTAATACCTGCAGCAAAGCCCCAAGC1590.15755677989615124No Hit
CAGGAAANNGCTATGANNCAGGGTGACTCCGATCCTGGAGGACAAGGCGG1580.1565658567521503No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG1540.1526021641761465No Hit
CAGGAAANNGCTATGANNGGGACCAGTCCAATAACCCCTGACAGGAAACA1540.1526021641761465No Hit
CAGGAAACNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1490.14764754845614173No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA1480.14665662531214077No Hit
CAGGAAANAGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT1460.1446747790241389No Hit
CAGGAAANNGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1460.1446747790241389No Hit
CAGGAAANNGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG1440.142692932736137No Hit
CAGGANANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1420.1407110864481351No Hit
CAGGAAANNGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT1410.13972016330413414No Hit
CAGGAAANAGCTATGANNCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC1390.13773831701613223No Hit
CAGGAAANNGCTATGANNCTTGGTGGAGCCATAGGCCGCATGCACGCGCA1380.13674739387213128No Hit
CAGGAAACAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1380.13674739387213128No Hit
CAGGAAANAGCTATGANNCTTGAGCTCCTCGGACACGGCAGGAAACAGCT1370.13575647072813032No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG1350.13377462444012841No Hit
CAGGAANNNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1350.13377462444012841No Hit
CAGGAAANNGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA1340.1327837012961275No Hit
NAGGANANNGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1320.13080185500812558No Hit
CAGGAAANAGCTATGANNTTGGACAGCACCTTGAATGCTATCAGGGAAGC1310.12981093186412462No Hit
CAGGANANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1280.12683816243212176No Hit
CAGGAAANNGCTATGANNCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA1270.1258472392881208No Hit
NAGGAAANNGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1270.1258472392881208No Hit
NAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1250.12386539300011891No Hit
CAGGAAANNGCTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC1240.12287446985611795No Hit
CAGGAAANAGCTATGANNCACGACACCCCACCTCTTGGACACTGCCCACC1210.1199017004241151No Hit
CAGGANANNGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1200.11891077728011415No Hit
NAGGAAANNGCTATGANNCCAGCAGAGCTCTTCTTACAGAGGCAGGAAAC1180.11692893099211227No Hit
CAGGAAANAGCTATGANNCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA1160.11494708470411036No Hit
CAGGAAANAGCTATGANNGCTGCTGACCCAACCCTACCCTGTCCAGCCCC1140.11296523841610845No Hit
CAGGAAANAGCTATGANNGCAGCTGGGAGATGCCTGGACTGGCCGTCGTT1140.11296523841610845No Hit
NAGGAAANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1130.11197431527210751No Hit
NAGGANANNGCTATGANNCCAGCAGAGCTCTTCTTACAGAGGCAGGAAAC1130.11197431527210751No Hit
CAGGAAANNGCTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG1120.11098339212810655No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG1120.11098339212810655No Hit
NAGGAAANNGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC1110.1099924689841056No Hit
CAGGAAANNGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1100.10900154584010464No Hit
NAGGANANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1090.10801062269610369No Hit
CAGGAAANNGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA1080.10701969955210275No Hit
CAGGAAACNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1080.10701969955210275No Hit
NAGGAAANAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT1070.1060287764081018No Hit
CAGGAAANAGCTATGANNAAGGACAAGGTGAGGCAGCACTTCTGCAGGGT1060.10503785326410084No Hit
CAGGAANNNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1040.10305600697609893No Hit
CAGGANANNGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC1040.10305600697609893No Hit
CAGGAAANAGCTATGANNTGGCCCCAGTGTTGAGAGGTCAGGAAACAGCT1040.10305600697609893No Hit
CAGGAAACAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT1040.10305600697609893No Hit
CAGGANANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1020.10107416068809703No Hit
CAGGAAANAGCTATGANNCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG1020.10107416068809703No Hit
CAGGAAANAGCTATGANNAAAGGCAGCAGGGTCGATGGCTGTGGGATGGA1020.10107416068809703No Hit
CAGGAAANNGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT1010.10008323754409608No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)