FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139816
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA62414.463723751215884No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC54953.9301653601876754No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG49893.568261143216799No Hit
TGTAAAACGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTCTGGCATGG45243.235681180980717No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA42873.0661726840991017No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG37792.702838015677748No Hit
TGTAAAACGACGGCCAGTTCCCAAAGGCTGGTGGAGGAGATGCTGAAGAT37412.6756594381186702No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC33012.3609601190135607No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG32712.339503347256394No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC32162.3001659323682553No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG31792.2737025805344167No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC30632.190736396406706No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA27531.9690164215826516No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC23941.7122503862218914No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT23881.7079590318704583No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT21261.5205698918578703No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT19051.3625050065800768No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT14141.011329175487784No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC13360.9555415689191509No Hit
TGTAAAACGACGGCCAGTTGGAGTGCCTGGGTTAGAACCCTGCTGGTACC13350.9548263431939119No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC13090.9362304743377009No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCCAAGAGGTGGGG12640.9040453167019511No Hit
TGTAAAACGACGGCCAGTAGTGACAACAGCCCCAAAGGAATGATCCCGCT12280.8782971905933513No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCATCAGGTTCCC11770.841820678606168No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC10640.7610001716541741No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA9910.7087886937117354No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC9270.6630142472964468No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCAGTGGTGCGATGGTCATAGCTG9160.655146764318819No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC8760.6265377353092636No Hit
TGTAAAACGACGGCCAGTCGCCTGAATAGGTGCAGGGGCATGATCACAGC8110.580048063168736No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT7790.5571608399610917No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCAAGAGGTGGGGT7040.5035189105681753No Hit
TGTAAAACGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGGGACACGGA6530.46704239858099217No Hit
TGTAAAACGACGGCCAGTCGCCTGAATAGGTGCAGGGGGCAATCATGGCT6490.46418149568003664No Hit
TGTAAAACGACGGCCAGTTGAAGAATGGTGGCTTGTTTCATGGTTGTAAA6000.4291354351433312No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG5760.411970017737598No Hit
TGTAAAACGACGGCCAGTCGCCTGAATAGGTGCAGGGGCACGAGCTCGGC5150.36834124849802596No Hit
TGTAAAACGACGGCCAGTCGCCTGAATAGGTGCAGGGGCACGATATCCAT4990.35689763689420384No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA4870.3483149281913372No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG4260.3046861589517652No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCAAGGGT4170.29824912742461523No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG4080.29181209589746526No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG3860.2760771299422098No Hit
TGTAAAACGACGGCCAGTCCTGCCGCCTGGTCTCACCTCGCCATGGTTCG3590.25676603536075987No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA3190.22815700635120442No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCCT2900.20741546031927677No Hit
TGTAAAACGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG2880.205985008868799No Hit
TGTAAAACGACGGCCAGTAGGTCATCCTCCTGCCTCGGCCTCCCAAAGTG2840.20312410596784344No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC2600.1859586885621102No Hit
TGTAAAACGACGGCCAGTTCCCACCACCTGCCTTCCGGTCATAGCTGTTT2590.18524346283687132No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC2500.17880643130972135No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGCATTTCCTTGGC2500.17880643130972135No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCCCTGCACATGCAAG2440.17451507695828802No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG2320.16593236825542143No Hit
TGTAAAACGACGGCCAGTCCTGCCGCCTGGTCTCACCTCGCTATGGTTCG2300.16450191680494364No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC2150.15377353092636037No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG2060.1473364993992104No Hit
TGTAAAACGACGGCTAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA1900.13589288779538822No Hit
TGTAAAACGACGGCTAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC1720.12301882474108829No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAAGGCGCGGAGGCAGC1660.11872747038965496No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCTCAACAAGGAGACGATG1640.1172970189391772No Hit
TGNAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAATCTCCACGCAGGA1630.11658179321393833No Hit
TGTAAAACGACGGCTAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG1620.11586656748869943No Hit
TGTAAAACGACGGCCAGTTCTTCACCTCCCAAAATGCTGGGATTACAGGC1610.11515134176346056No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA1610.11515134176346056No Hit
TGTAAAACGACGGCCAGTGAACAGCCCCGGAAGCAAGTAAAAGGAGGAGG1530.10942953596154946No Hit
TGTAAAACGACGGCCAGTCGCCTGAATAGGTGCAGGGGCGCAATCATGGC1490.10656863306059393No Hit
TGNAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC1470.10513818161011616No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCGTTTGTATCAGG1470.10513818161011616No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAACACCTCCACGCAGGA1440.1029925044343995No Hit
TGTAAAACGACGGCCAGTTGAAGAATGGTGGCCTTTATGGAATGAGACGC1440.1029925044343995No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT1400.10013160153344396No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)