FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139816
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT30242.1628425931223894No Hit
CAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG26461.8924872689820909No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT26361.8853350117297016No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT20741.4833781541454483No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC18781.3431939119986267No Hit
CAGGAAANAGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT17611.2595125021456772No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA16251.1622418035131887No Hit
CAGGAAANAGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC16231.160811352062711No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC15161.0842821994621503No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG12970.9276477656348344No Hit
CAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT12240.8754362876923957No Hit
CAGGAAANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT11080.792470103564685No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT10140.7252388853922298No Hit
CAGGAAANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG9820.7023516621845854No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT9060.6479945070664301No Hit
CAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT8620.6165245751559192No Hit
CAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT7750.5542999370601362No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG7520.5378497453796418No Hit
CAGGAAANNGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT7000.5006580076672198No Hit
CAGGAAANAGCTATGANNCAGGGTGACTCCGATCCTGGAGGACAAGGCGG6930.4956514275905476No Hit
CAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC6830.4884991703381587No Hit
CAGGAAANAGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG6560.4691880757567088No Hit
CAGGAAANNGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC6350.4541683355266922No Hit
CAGGAAANAGCTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC5870.4198375007152257No Hit
CAGGAAANNGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC5620.40195685758425365No Hit
CAGGAAANNGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA5510.39408937460662585No Hit
CAGGAAANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG5170.36977169994850373No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA4710.336871316587515No Hit
CAGGAAANAGCTATGANNCCCAAACCTGCGGAGGCACGGGGATGGCAGTG4680.3347256394117984No Hit
CAGGAAANAGCTATGANNCCTTGGGCAAGTTAACAAACCTCTCTGAGCCT4530.32399725353321507No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG4380.3132688676546318No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA4140.29610345024889856No Hit
CAGGAAANAGCTATGANNATCGCACCACTGCACTCCAGCACTGGCCGTCG4120.2946729987984208No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA3950.28251416146935976No Hit
CAGGAAANAGCTATGANNCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT3640.26034216398695426No Hit
CAGGAAANNGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT3440.24603764948217657No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA3420.24460719803169878No Hit
CAGGANANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT3360.2403158436802655No Hit
CAGGAAANAGCTATGANNGGAGGCTGAGGTGGGAGGATCAACTGAACCCA3320.23745494077930993No Hit
CAGGAAANAGCTATGANNCACGACACCCCACCTCTTGGACACTGCCCACC3270.23387881215311554No Hit
CAGGAAANAGCTATGANNTTGGACAGCACCTTGAATGCTATCAGGGAAGC3260.23316358642787666No Hit
CAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3230.23101790925215995No Hit
CAGGAAANAGCTATGANNCTGGCCTCCTTCAATGCATTTAATGGCCTTAT3170.22672655490072668No Hit
CAGGAAANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT3170.22672655490072668No Hit
NAGGANANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT3090.22100474909881557No Hit
NAGGAAANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT3090.22100474909881557No Hit
NAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3080.2202895233735767No Hit
CAGGAAANNGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT3010.2152829432969045No Hit
CAGGAAANAGCTATGANNAGCGTCTCATTCCATAAAGGCCACCATTCTTC3000.2145677175716656No Hit
NAGGAAANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2780.19883275161641012No Hit
CAGGAAANAGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT2670.19096526863878238No Hit
NAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2650.18953481718830462No Hit
CAGGANANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2640.18881959146306576No Hit
CAGGAAANNGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG2630.18810436573782685No Hit
CAGGAAANAGCTATGANNGGAGGCTGAGGTGGGAGGATCCCTTGAATCTA2620.18738914001258797No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG2590.18524346283687132No Hit
CAGGAAANAGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA2580.18452823711163244No Hit
NAGGANANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2480.17737597985924358No Hit
CAGGAAANAGCTATGANNTTGGACAGCACCTTGAATGCTTTCAGGGAAGC2380.17022372260685473No Hit
CAGGAAANNGCTATGANNCAGGGTGACTCCGATCCTGGAGGACAAGGCGG2360.16879327115637696No Hit
CAGGAAACAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2350.16807804543113805No Hit
CAGGAAANAGCTATGANNGGAGGCTGAGGTGGGAGGATCGCTTGATCCCG2350.16807804543113805No Hit
CAGGAAANNGCTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC2350.16807804543113805No Hit
CAGGAAANNGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG2300.16450191680494364No Hit
NAGGANANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2270.162356239629227No Hit
CAGGANANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2260.1616410139039881No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT2170.15520398237683813No Hit
CAGGAAACNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2140.15305830520112146No Hit
CAGGANANNGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT2130.1523430794758826No Hit
CAGGAAANAGCTATGANNTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC2100.15019740230016593No Hit
CAGGAAANNGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA2100.15019740230016593No Hit
CAGGAAANAGCTATGANNGGAGGCTGAGGTGGGAGGATCACTTGAGCCTG2060.1473364993992104No Hit
CAGGANANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1960.14018424214682154No Hit
CAGGAAANAGCTATGANNGGCTGGCACAAAGAACAGCACTGACTGTTTAT1950.13946901642158266No Hit
CAGGAAANAGCTATGANNTCCCTTAAACTTGGGATTGCTCCATTGCACTC1910.13660811352062713No Hit
NAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1890.13517766207014933No Hit
NAGGAAANNGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT1880.13446243634491045No Hit
NAGGANANNGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT1860.1330319848944327No Hit
CAGGAAANNGCTATGANNATCGCACCACTGCACTCCAGCACTGGCCGTCG1840.13160153344395492No Hit
NAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1830.13088630771871604No Hit
CAGGAANNNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1820.13017108199347713No Hit
CAGGAAANNGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1820.13017108199347713No Hit
NAGGANANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1790.12802540481776048No Hit
NAGGANANNGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA1760.12587972764204383No Hit
CAGGAAANNGCTATGANNCCCAAACCTGCGGAGGCACGGGGATGGCAGTG1740.12444927619156607No Hit
CAGGAAACAGCTATGANCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1740.12444927619156607No Hit
CAGGAANNNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1740.12444927619156607No Hit
CAGGAAACAGCNATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1730.12373405046632717No Hit
CAGGAAACNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1700.12158837329061052No Hit
CAGGANANNGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC1640.1172970189391772No Hit
NAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1640.1172970189391772No Hit
CAGGAANNNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1640.1172970189391772No Hit
CAGGAAACAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT1640.1172970189391772No Hit
NAGGAAANNGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA1630.11658179321393833No Hit
CAGGAAANAGCTATGANNCTGGCCTCCTTCAATGCATTTAATGGCCTTCT1620.11586656748869943No Hit
CAGGAAANNGCTATGANNCCTTGGGCAAGTTAACAAACCTCTCTGAGCCT1620.11586656748869943No Hit
CAGGAAANNGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA1610.11515134176346056No Hit
NAGGAAANNGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC1600.11443611603822167No Hit
NAGGANANNGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC1580.11300566458774389No Hit
NAGGAAANAGCTATGANCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1530.10942953596154946No Hit
NAGGAAANNGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC1510.1079990845110717No Hit
CAGGAAACAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG1510.1079990845110717No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG1500.1072838587858328No Hit
CAGGAAANNGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1500.1072838587858328No Hit
CAGGANANNGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA1490.10656863306059393No Hit
CAGGAAACAGCNATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1470.10513818161011616No Hit
NAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1470.10513818161011616No Hit
CAGGAAACAGCNATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1460.10442295588487727No Hit
NAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1450.10370773015963838No Hit
CAGGAAANAGCTATGANNCTTGAGCTCCTCGGACACGGCAGGAAACAGCT1450.10370773015963838No Hit
CAGGAAANNGCTATGANNCACGACACCCCACCTCTTGGACACTGCCCACC1440.1029925044343995No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)