FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106754
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT10100.9461003803136182No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT9910.9283024523671244No Hit
CAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG9750.913314723570077No Hit
CAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT8870.8308822151863161No Hit
CAGGAAANAGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT8040.7531333720516328No Hit
CAGGAAANAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT7960.7456395076531089No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT7890.7390823763044008No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT7660.7175375161586451No Hit
CAGGAAANAGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC7030.658523334020271No Hit
CAGGAAANAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT6590.6173070798283905No Hit
CAGGAAANAGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA6400.5995091518818967No Hit
CAGGAAANAGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT6390.5985724188320812No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT5740.5376847705940762No Hit
CAGGAAANAGCTATGACCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT5720.5358113044944453No Hit
CAGGAAANAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG5710.5348745714446298No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT5630.527380707046106No Hit
CAGGAAANAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAACT5610.525507240946475No Hit
CAGGAAANAGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC5490.5142664443486895No Hit
CAGGAAANAGCTATGANNCATGGTTGGGGAAAAACTCCTCATCTTCATGT5330.4992787155516421No Hit
CAGGAAANAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT5230.4899113850534874No Hit
CAGGAAANAGCTATGANNCATGGTTGGGGAAAAACTCCTCATTTTCATGT5190.4861644528542256No Hit
CAGGAAANAGCTATGACCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC5190.4861644528542256No Hit
CAGGAAANAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAATT4960.46461959270847No Hit
CAGGAAANAGCTATGANNGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT4960.46461959270847No Hit
CAGGAAANAGCTATGANNGCTTCTATATGAGCGCATCTCACCTGAAGTTG4690.4393278003634525No Hit
CAGGAAANAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT4450.41684620716788123No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT4210.39436461397231015No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT4180.3915544148228638No Hit
CAGGAAANAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA4180.3915544148228638No Hit
CAGGAAANAGCTATGANNGCAAAACACCTTAACACTCGGCTTCTATTTGC4020.3765666860258164No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA3990.37375648687637No Hit
CAGGAAANAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT3960.37094628772692356No Hit
CAGGAAANAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG3920.36719935552766175No Hit
CAGGAAANAGCTATGANCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT3810.35689529197969166No Hit
CAGGAAACAGCTATGACCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT3760.3522116267306143No Hit
CAGGAAANAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC3580.335350431833936No Hit
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG3490.3269198343855968No Hit
CAGGAAANAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT3490.3269198343855968No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAACT3410.31942596998707307No Hit
CAGGAAANAGCTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT3370.31567903778781126No Hit
CAGGAAANAGCTATGACCCATGGTTGGGGAAAAACTCCTCATTTTCATGT3350.3138055716881803No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAATT3330.3119321055885494No Hit
CAGGAAANAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT3280.3072484403394721No Hit
CAGGAAANAGCTATGACCCATGGTTGGGGAAAAACTCCTCATCTTCATGT3190.2988178428911329No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT3180.29788110984131744No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTATGTGCA3100.2903872454427937No Hit
CAGGAAACAGCTATGACCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC3080.2885137793431628No Hit
CAGGAAANAGCTATGANCTGGGGATGAGGACAGCAGGGCAGTCACCATCT3070.28757704629334735No Hit
CAGGAAANAGCTATGACCGCTTCTATATGAGCGCATCTCACCTGAAGTTG3070.28757704629334735No Hit
CAGGAAANAGCTATGANNTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG3060.2866403132435319No Hit
CAGGAAANAGCTATGANCGCATCAGATTCACTTCCACTGTCGTCAGAATT3010.2819566479944545No Hit
CAGGAAANAGCTATGANCGCATCAGATTCACTTCCACTGTCGTCAGAACT2920.27352605054611534No Hit
CAGGAAANAGCTATGACCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT2910.2725893174962999No Hit
CAGGAAANAGCTATGANNTGAAGTGGCTCCATCCTTCCATTTGTTTATAT2890.270715851396669No Hit
CAGGAAANAGCTATGANNAAACTATTAATACCTGCAGCAAAGCCCCAAGC2780.2604117878486989No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA2750.2576015886992525No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT2720.25479138954980607No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2680.25104445735054426No Hit
CAGGAAANAGCTATGANCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC2650.24823425820109787No Hit
CAGGAAANAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT2640.24729752515128237No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA2550.23886692770294324No Hit
CAGGAAANAGCTATGANNTCCCACCACTGACTCCAGGGGAAGGAGGAGGA2550.23886692770294324No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2540.23793019465312773No Hit
CAGGAAACAGCTATGACCCATGGTTGGGGAAAAACTCCTCATCTTCATGT2510.23511999550368134No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT2480.232309796354235No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC2450.2294995972047886No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT2360.22106899975644942No Hit
CAGGAAANAGCTATGANNAACCAACACTGCTGCTAATTGTGTTTGTGTCC2350.22013226670663397No Hit
CAGGAAANAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA2350.22013226670663397No Hit
CAGGAAANAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC2310.21638533450737207No Hit
CAGGAAANAGCTATGANCGGGACCAGTCCAATAACCCCTGAGGGGTATAT2290.21451186840774117No Hit
CAGGAAANAGCTATGANCGCTTCTATATGAGCGCATCTCACCTGAAGTTG2250.21076493620847933No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT2220.2079547370590329No Hit
CAGGAAACAGCTATGACCCATGGTTGGGGAAAAACTCCTCATTTTCATGT2210.20701800400921744No Hit
CAGGAAANAGCTATGANCCATGGTTGGGGAAAAACTCCTCATTTTCATGT2160.20233433876014015No Hit
CAGGAAANAGCTATGANCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA2130.19952413961069376No Hit
CAGGAAANAGCTATGANNCTTGGTGGAGCCATAGGCCGCATGCACGCGCA2110.1976506735110628No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA2070.19390374131180096No Hit
CAGGAAANAGCTATGANNCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA2070.19390374131180096No Hit
CAGGAAANAGCTATGANNTCCTCTTCATTTTCACGGTGCCATTCCGATTC2070.19390374131180096No Hit
CAGGAAANAGCTATGANCCATGGTTGGGGAAAAACTCCTCATCTTCATGT2060.1929670082619855No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCAGAGGGAAGGTGACT2040.19109354216235455No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA2040.19109354216235455No Hit
CAGGAAANAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA2010.1882833430129082No Hit
CAGGAAANAGCTATGANNTGACAGCACAAGGAACACTGACCTACAGATAA1960.18359967776383088No Hit
CAGGAAANAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA1960.18359967776383088No Hit
CAGGAAACAGCTATGACCGCTTCTATATGAGCGCATCTCACCTGAAGTTG1950.1826629447140154No Hit
CAGGAAANAGCTATGANCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT1950.1826629447140154No Hit
CAGGAAANAGCTATGANCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC1930.18078947861438446No Hit
CAGGAAANAGCTATGACCTGAAGTGGCTCCATCCTTCCATTTGTTTATAT1920.179852745564569No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT1910.17891601251475356No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC1880.17610581336530715No Hit
CAGGAAANAGCTATGANNTCCAGTAAAACTGCCTCTGTTTCAACTCCCTT1840.17235888116604528No Hit
CAGGAAANAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1830.17142214811622983No Hit
CAGGAAANAGCTATGACCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1830.17142214811622983No Hit
CAGGAAANAGCTATGANCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT1750.16392828371770612No Hit
CAGGAAANAGCTATGACCAAACTATTAATACCTGCAGCAAAGCCCCAAGC1700.1592446184686288No Hit
CAGGAAANAGCTATGANNTCCTCTTCATTTTCACGGTGCCATTCTGATTC1690.15830788541881335No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1690.15830788541881335No Hit
CAGGAAANAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG1680.15737115236899787No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC1660.15549768626936694No Hit
CAGGAAANAGCTATGANNTGCCCAGTTGTTTCCAGAACCTTGTTTTTGAC1650.1545609532195515No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1650.1545609532195515No Hit
CAGGAAANAGCTATGANCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT1590.14894055492065872No Hit
CAGGAAANAGCTATGANNGCATCCTGCTTAGTGCTGCCTCAATCTGGTCC1570.14706708882102779No Hit
CAGGAAANAGCTATGACCAACCAACACTGCTGCTAATTGTGTTTGTGTCC1540.1442568896715814No Hit
CAGGAAANAGCTATGANCGCAAAACACCTTAACACTCGGCTTCTATTTGC1530.14332015662176595No Hit
CAGGAAANAGCTATGANNAAGTTGGTCAGCCCTGGTGACTCATTGGATAT1520.14238342357195047No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA1520.14238342357195047No Hit
CAGGAAANAGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT1460.13676302527305767No Hit
CAGGAAANAGCTATGANNGCTGCTGACCCAACCCTACCCTGTCCAGCCCC1460.13676302527305767No Hit
CAGGAAANAGCTATGANNGGCAGACAGCCTCTCCTCCTTTCATCACAAAA1450.13582629222324222No Hit
CAGGAAANAGCTATGACCTGACAGCACAAGGAACACTGACCTACAGATAA1420.13301609307379583No Hit
CAGGAAANAGCTATGANNAAGGACAAGGTGAGGCAGCAGTAATGGACTGG1410.13207936002398035No Hit
CAGGAAANAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1380.129269160874534No Hit
CAGGAAANAGCTATGANNCCTGGACGCAATCACCCAGGCCAGCTAAGTGA1370.12833242782471851No Hit
CAGGAAANAGCTATGACCTCCTCTTCATTTTCACGGTGCCATTCCGATTC1360.12739569477490303No Hit
CAGGAAACAGCTATGACCTGAAGTGGCTCCATCCTTCCATTTGTTTATAT1360.12739569477490303No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1350.12645896172508758No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1330.12458549562545666No Hit
CAGGAAANAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT1330.12458549562545666No Hit
CAGGAAANAGCTATGANNAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC1320.12364876257564118No Hit
CAGGAAANAGCTATGACCACCAAGACCCCAGCAGCAGAGGGAAGGTGACT1300.12177529647601026No Hit
CAGGAAANAGCTATGACCTCCCACCACTGACTCCAGGGGAAGGAGGAGGA1260.1180283642767484No Hit
CAGGAAANAGCTATGACCCTTGGTGGAGCCATAGGCCGCATGCACGCGCA1250.11709163122693295No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC1250.11709163122693295No Hit
CAGGAAANAGCTATGANCTTCATACAGAGATACAAAGGCAACTATGTGCA1240.1161548981771175No Hit
CAGGAAANAGCTATGANCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1240.1161548981771175No Hit
CAGGAAANAGCTATGANCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1240.1161548981771175No Hit
CAGGAAANAGCTATGANNCGCTTCTGGGGACTTTGCTCTTGGGCTCTTTT1230.11521816512730203No Hit
CAGGAAANAGCTATGANNTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC1210.11334469902767108No Hit
CAGGAAANAGCTATGACCTCCAGTAAAACTGCCTCTGTTTCAACTCCCTT1190.11147123292804016No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC1190.11147123292804016No Hit
CAGGAAACAGCTATGACCAAACTATTAATACCTGCAGCAAAGCCCCAAGC1180.11053449987822471No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG1180.11053449987822471No Hit
CAGGAAACAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG1170.10959776682840924No Hit
CAGGAAANAGCTATGANNGCTTCTATATGAGCGCATCTCACCTGAATTTG1160.10866103377859376No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA1150.10772430072877831No Hit
CAGGAAANAGCTATGANNCCCTGCGGCTCCTTTCAAGTTAAGTCACCACT1140.10678756767896284No Hit
CAGGAAACAGCTATGACCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1130.10585083462914739No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA1130.10585083462914739No Hit
CAGGAAANAGCTATGACCCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA1100.10304063547970099No Hit
CAGGAAANAGCTATGACCTCCTCTTCATTTTCACGGTGCCATTCTGATTC1100.10304063547970099No Hit
CAGGAAANAGCTATGACCTGCCCAGTTGTTTCCAGAACCTTGTTTTTGAC1080.10116716938007007No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC1080.10116716938007007No Hit
CAGGAAANAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT1080.10116716938007007No Hit
CAGGAAANAGCTATGANCTGAAGTGGCTCCATCCTTCCATTTGTTTATAT1070.10023043633025461No Hit
CAGGAAANAGCTATGANCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1070.10023043633025461No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)