FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97715
Sequences flagged as poor quality0
Sequence length300
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA20532.1010080335670063No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAGTGCCAAGG18001.8420917975745792No Hit
TGTAAAACGACGGCCAGTACTAAACTGTAGTGAACAGAGACATGCCCAGG15751.6118303228777566No Hit
TGTAAAACGACGGCCAGTCCACCTGCTTCGCACCATCTCCATCTCCATTT14871.5217725016629995No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTGTAAAACGACGG14291.4624162104078187No Hit
TGTAAAACGACGGCCAGTACAAAAGTTCTCCAAGTCCCTAGATTAGCTAG14181.4511589827559739No Hit
TGTAAAACGACGGCCAGTCGCACAGTCTGTTCTGCAGCCCTGGAGCAAGG13071.3375633219055416No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGGCTCCAGGATCG12401.2689965716624878No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGGCAGCACCTCT11481.174845213119787No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCCAAGAGGTGGGG11121.1380033771682956No Hit
TGTAAAACGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGATTTGGGGC10901.1154889218646062No Hit
TGTAAAACGACGGCCAGTGCCAATCCTGAGGCCATCAACACAAGGACTGG10411.0653430895972984No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA10271.051015708949496No Hit
TGTAAAACGACGGCCAGTCCCAGCACAACTTCCCCCATGTAAAACGACGG10061.0295246379777925No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC9700.992682802026301No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC9480.9701683467226117No Hit
TGTAAAACGACGGCCAGTTCTTTGGCTCTACCTAAAAGTTGGTCAGCAAA9450.9670981937266541No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA9050.926162820447219No Hit
TGTAAAACGACGGCCAGTCCTGGCCCACAAACTGTGCTGGGGTCATAGCT9020.9230926674512613No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG8890.9097886711354449No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT8790.8995548278155862No Hit
TGTAAAACGACGGCCAGTCTTGCACTACATGGCCATCAGGGTCTCGTCCC8290.8483856112162923No Hit
TGTAAAACGACGGCCAGTTGAAGAGAGAGGTTAGCAAGGGAGGACTCCAG8110.8299646932405464No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCGAGCAGGTGGC8070.8258711559126031No Hit
TGTAAAACGACGGCCAGTCAGCCAGTAGCAACAGCAGCTACTGATGTAAG7960.8146139282607583No Hit
TGTAAAACGACGGCCAGTTTGCTGCTGGGCTTCCTCTGCACTTGAAAGGA7810.7992631632809702No Hit
TGTAAAACGACGGCCAGTAGTCAGCCACGTTGGAACCAGTACTCACGCAA7600.7777720923092668No Hit
TGTAAAACGACGGCCAGTGGCTCCCCAGCTGTCTCTCTTGTAAAACGACG7360.7532108683416058No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGCAGGATGGAG7280.7450237936857187No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGTGTCGTGGGGCA6690.6846441180985519No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCAGGCCCCAAGGA6670.6825973494345802No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCACATGCCTCCC6430.6580361254669191No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGAAGCCCAGTCAGG6400.6549659724709614No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCCGGCCCCAAGGA6280.6426853604871309No Hit
TGTAAAACGACGGCCAGTATTCCTCCACTATCGCCCCTACGTAAAGAAAT6230.6375684388272016No Hit
TGTAAAACGACGGCCAGTGGAGCGACTTGACAATGCCCCTCACACCCCTT6110.625287826843371No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCAAGAGGTGGGGT6010.6150539835235123No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC5860.5997032185437241No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGTCCAACAATGGCCTG5630.576165378908049No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCCCTCGGAGCTG5470.5597912295962749No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTCACCATTTGGTG5320.5444404646164868No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGTCATAGCTGTT5240.5362533899605998No Hit
TGTAAAACGACGGCCAGTAGATCATCCAGCGCACACGGAGACAGGCCCAG5120.5239727779767692No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGGTCTGTGCGG5000.5116921659929387No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA4870.49838816967712224No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGCGTGGGGCACTC4860.4973647853451364No Hit
TGTAAAACGACGGCCAGTCGCACAGTCTGTTCTGCAGCCACAGCACACGT4800.4912244793532211No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC4700.48099063603336234No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCAACCGT4670.4779204830374047No Hit
TGTAAAACGACGGCCAGTAGATCATCCAGCGCACACGGCGTCTTGGCGGG4650.475873714373433No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG4640.47485033004144706No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC4530.46359310238960244No Hit
TGTAAAACGACGGCCAGTAAGGGTGGGTCATGGGGTGACACCGTAACTGT4450.45540602773371536No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCTCCTGCTCAGCCTT4410.45131249040577187No Hit
TGTAAAACGACGGCCAGTCCCAGCACAACTTCCCCCATGGCGTGGTGTCC4360.44619556874584254No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGGGCCCCGGGAGC4320.44210203141789894No Hit
TGTAAAACGACGGCCAGTCTGGGTTGGGGTGTCTGCTGCTCCAGGATGGC4310.4410786470859131No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAGGAGGACCC4230.43289157243002613No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCCACGCTGGGAAT4190.42879803510208253No Hit
TGTAAAACGACGGCCAGTCTGGGTTGGGGTGTCTGCTGGGAGGTACAGGG4080.4175408074502379No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCCCCCAACTCCA3990.40833034846236504No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT3970.4062835797983933No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTACCCAGTGGTGTG3930.40219004247044976No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT3800.3888860461546334No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGAAGCCTCAACCC3780.3868392774906616No Hit
TGTAAAACGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT3750.383769124494704No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCAGGGCCATGTG3610.3694417438469017No Hit
TGTAAAACGACGGCCAGTCTGGGTTGGGGTGTCTGCTGTTTGTGCAGGCG3540.3622780535230006No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCGCAGCCCTCCTAC3530.3612546691910147No Hit
TGTAAAACGACGGCCAGTCTGGGTTGGGGTGTCTGCTGTCTTCACTGTTG3510.35920790052704293No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCGTGACAAGGCT3440.3520442102031418No Hit
TGTAAAACGACGGCCAGTGCCCTGAACCGGTGCCAAATAATGACACTGTG3420.34999744153917006No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC3340.34181036688328303No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGTGTGTGTTCGTGC3330.34078698255129714No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTCCATTTGGTGTG3310.3387402138873254No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGTCATAGCTGTTT3110.31827252724760785No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC3100.31724914291562195No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG3040.31110883692370667No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC3030.3100854525917208No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG3000.3070152995957632No Hit
TGTAAAACGACGGCCAGTACACTCCCTCCACCTCCCATCCCAGAAAAAGT2950.3018983779358338No Hit
TGTAAAACGACGGCCAGTCTTGCACTACATGGCCATCACCCCCTCTCCAC2930.29985160927186205No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGGATGCTGAGTGGGAG2920.29882822493987615No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC2900.2967814562759044No Hit
TGTAAAACGACGGCCAGTTGAGAAGCTGAGGCTTAAAGAGGGATCCCTTG2760.28245407562810215No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGGTCCCCGGATGCC2740.2804073069641304No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCTGCAGACAGGAT2710.27733715396817277No Hit
TGTAAAACGACGGCCAGTAGATCATCCAGCGCACACGGGGAGACAGGCCC2690.275290385304201No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC2680.2742670009722151No Hit
TGTAAAACGACGGCCAGTATTCCTCCACTATCGCCCCTGTGTTGGCCAAC2620.2681266949802999No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCAAGGGT2560.2619863889883846No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAATTAGTAGT2540.25993962032441287No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGGGAGGATCCTC2520.25789285166044107No Hit
TGTAAAACGACGGCCAGTCCAGGCATCTCCCAGCTGCGGTCATAGCTGTT2500.2558460829964693No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTGTGAGGAGAATG2450.25072916133653994No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTTGGGAAGGGA2430.2486823926725682No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC2410.24663562400859645No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGGGCGGCGGCTCCT2390.24458885534462466No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGCCTCAGGGCTT2370.2425420866806529No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGCTGAGGGAGGAT2340.23947193368469527No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCCAGGGGGCGTCCCGG2320.23742516502072353No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGAAAGCCTCAGGCA2260.2312848590288083No Hit
TGTAAAACGACGGCCAGTCCCAGCACAACTTCCCCCATGGAACAGAAACG2260.2312848590288083No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCCCACAGACCCC2240.2292380903648365No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGGGTCAGTGGGAGTCT2150.22002763137696363No Hit
TGTAAAACGACGGCCAGTAGATCATCCAGCGCACACGGAGGAGACAGGCC2090.21388732538504834No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGTGGGAGTCTGGCTAC2070.2118405567210766No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTGACCCTTCCAGGA2060.2108171723890907No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCATGACAAGGCT2050.20979378805710486No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCTCAGCCTCCTGCTCA2040.20877040372511896No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGGGAAGGAGAGT1980.2026300977332037No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCAGGATGGAGGG1960.20058332906923193No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGAGGATCCTCCC1950.1995599447372461No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATTGAAAATCTGC1950.1995599447372461No Hit
TGTAAAACGACGGCCAGTAGATCATCCAGCGCACACGGGAGACAGGCCCA1870.19137287008135906No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCCCCCAGGATGG1800.18420917975745793No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGTGGGGTCACCCC1800.18420917975745793No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGAGGCTTTTTGTTCCA1800.18420917975745793No Hit
TGTAAAACGACGGCCAGTAGCCTCTGGACACCCAGGACCTGATTCCCAGG1780.18216241109348616No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCAGCCCCAAGGAT1740.17806887376554265No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCCGCCCCAAGGAT1630.166811646113698No Hit
TGTAAAACGACGGCCAGTTTGCTGCTGGGCTTCCTCTGGGCTGCTCTGTC1580.16169472445376862No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT1570.16067134012178272No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC1560.15964795578979685No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGGTGGCCCTTC1510.15453103412986746No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGCGTGTGTTCGTGC1490.15248426546589572No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGACACTGTGAGGCCC1470.15043749680192395No Hit
TGTAAAACGACGGCCAGTAGATCATCCAGCGCACACGGCAGGAGACAGGC1470.15043749680192395No Hit
TGTAAAACGACGGCCAGTAGATCATCCAGCGCACACGGTGAGCACAGGAG1450.1483907281379522No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGCTGGGGCCGGA1450.1483907281379522No Hit
TGTAAAACGACGGCCAGTCCCAGCACAACTTCCCCCATTGTAAAACGACG1410.1442971908100087No Hit
TGTAAAACGACGGCCAGTTGACACAGCTGCTTCTGAGTACAAGTATGTAG1340.13713350048610756No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGACCAACAATGGCCTG1300.13303996315816405No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT1300.13303996315816405No Hit
TGTAAAACGACGGCCAGTAAGGGTGGGTCATGGGGTGAGGGTGGGTCGCG1280.1309931944941923No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCCCTCAGAGCTG1270.12996981016220643No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCATCAGGTTCCC1260.12894642583022053No Hit
TGTAAAACGACGGCCAGTGCCCAAGGTTGGTGAGATGGGCTATGACCTCT1240.1268996571662488No Hit
TGTAAAACGACGGCCAGTTCATAGCTGTGAAGAGGGATACCAAGGAAAGT1240.1268996571662488No Hit
TGTAAAACGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT1230.12587627283426292No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTCCTCACTCGGAT1180.12075935117433353No Hit
TGTAAAACGACGGCCAGTAACAAATTCAGGTGAGATGCGCTCACATAGAA1170.11973596684234764No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGGCGGCACCTCT1140.11666581384639002No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC1130.11564242951440415No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGGCTTGACACGG1130.11564242951440415No Hit
TGTAAAACGACGGCCAGTGACCTCATGGGGCGCTTCCTGGGAGAGCTTCG1090.11154889218646064No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCCAGTGCCTCCG1070.10950212352248886No Hit
TGTAAAACGACGGCCAGTGCCCAAGGTTGGTGAGATGGGGCCAGGTCATA1050.10745535485851712No Hit
TGTAAAACGACGGCCAGTTCTATTGCTGAGACTTTCCAGAGCAGTGGGGG1030.10540858619454535No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGCTGAGCGGGTTC1020.10438520186255948No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTTTAATGGAGTAA1010.10336181753057361No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCTGAGAAGGACCT1010.10336181753057361No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACTGCCTTCCAGCATTT1000.10233843319858774No Hit
TGTAAAACGACGGCCAGTCCTTGAAGGCTGGGCTTCTGTCTTCTGGCAGG980.10029166453461597No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)