FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003245839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162777
Sequences flagged as poor quality0
Sequence length300
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAGTGCCAAGG29781.8294967962304256No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGACGGGTGGCC26911.6531819605963987No Hit
TGTAAAACGACGGCCAGTAAGAGGTGAGCGAGGTCTGCGCCACGTGATCC26771.6445812369069341No Hit
TGTAAAACGACGGCCAGTTGGAGTGCCTGGGTTAGAACCCAGGCATTCAA24471.5032836334371564No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTGTAAAACGACGG23731.4578226653642714No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGCCCCAATCCTGTCTC23321.4326348317022675No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG22301.369972416250453No Hit
TGTAAAACGACGGCCAGTCGCACAGTCTGTTCTGCAGCCCTGGAGCAAGG21521.3220540985520068No Hit
TGTAAAACGACGGCCAGTGCCACTTCTGAAGGAGGTTCCCCCAGCAGTGC21181.3011666267347353No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTAGAGCCTGGCT19781.215159389840088No Hit
TGTAAAACGACGGCCAGTCTTGCACTACATGGCCATCAGGGTCTCGTCCC18841.1574116736393962No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCACAGTGACCTGT18761.1524969743882734No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC18031.1076503437217788No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTGGGGTGCT16641.022257444233522No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA15610.9589806913753172No Hit
TGTAAAACGACGGCCAGTGCCAATCCTGAGGCCATCAACACAAGGACTGG15110.9282638210558003No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT15040.9239634592110678No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG14230.8742021292934505No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC14130.8680587552295471No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGCACGCAGGCT14080.8649870681975955No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC13530.8311985108461268No Hit
TGTAAAACGACGGCCAGTAGTCAGCCACGTTGGAACCAGTACTCACGCAA13380.8219834497502718No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCGAGCAGGTGGC13360.8207547749374912No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGTGCTGCTGAATG12590.7734507946454351No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCAGGGCTTGAACCC12220.7507203106089927No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC11900.7310615136045019No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG11820.7261468143533791No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCAGGCCCCAAGGA11500.7064880173488883No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGAAGCCCAGTCAGG11210.6886722325635686No Hit
TGTAAAACGACGGCCAGTATTCCTCCACTATCGCCCCTACGTAAAGAAAT11120.6831431959060555No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG11020.6769998218421521No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC10890.6690134355590778No Hit
TGTAAAACGACGGCCAGTTCTTTGGCTCTACCTAAAAGTTGGTCAGCAAA10830.6653274111207358No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCAGGTGACTCCAG10300.6327675285820478No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGTCCAACAATGGCCTG10290.6321531911756575No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTCACCATTTGGTG10060.6180234308286797No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC9850.6051223452944826No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA9670.5940642719794565No Hit
TGTAAAACGACGGCCAGTAGATCATCCAGCGCACACGGCGTCTTGGCGGG9620.5909925849475048No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGGTGCTCTGGT9550.5866922231027725No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCCCTCGGAGCTG9510.5842348734772111No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCCGGCCCCAAGGA9310.5719481253494043No Hit
TGTAAAACGACGGCCAGTGGAGCGACTTGACAATGCCCCTCACACCCCTT9050.5559753527832556No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT8790.5400025802171069No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATCACATTTCAGCA8640.5307875191212518No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATCGCATTTCAGCA8490.5215724580253966No Hit
TGTAAAACGACGGCCAGTATTCCTCCACTATCGCCCCTGTGTTGGCCAAC8310.5105143847103706No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTGCCCAGTGTGTT8220.5049853480528577No Hit
TGTAAAACGACGGCCAGTCGCACAGTCTGTTCTGCAGCCACAGCACACGT8030.4933129373314412No Hit
TGTAAAACGACGGCCAGTCCAGAGATCAATAATAGCTCAGGGGAGATGGA7800.4791831769844634No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCGCAGCCCTCCTAC7690.4724254655141697No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCCGAGTAGCTGAG7590.46628209145026633No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGCCTTCGGAGGC7540.4632104044183146No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC7530.46259606701192435No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGATCCCCGGTG7470.4589100425735823No Hit
TGTAAAACGACGGCCAGTAGATCATCCAGCGCACACGGAGACAGGCCCAG7290.4478519692585562No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGGGAGGATCCTC7110.43679389594353013No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCCACGCTGGGAAT6840.4202067859709909No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAGGAGGACCC6710.41222039968791657No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCAGGGCCATGTG6650.40853437524957453No Hit
TGTAAAACGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT6540.4017766637792809No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG6530.40116232637289045No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA6490.3987049767473292No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGTTCGGAGGCAGG6450.3962476271217678No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC6350.39010425305786445No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGAAGCCTCAACCC6320.3882612408386934No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCCTCGGAGCTTC6310.3876469034323031No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG6250.3839608789939611No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT6180.3796605171492287No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGGGCCCCGGGAGC5910.36307340717668957No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC5880.3612303949575186No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGGGCGGCGGCTCCT5740.35262967126805383No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCAGGAAATCAGGCT5740.35262967126805383No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGTGGGAGTCTGGCTAC5740.35262967126805383No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCAGAAATCAGGCTG5480.33665689870190507No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCACCCCCTGCCA5270.32375581316770796No Hit
TGTAAAACGACGGCCAGTACACTCCCTCCACCTCCCATCCCAGAAAAAGT5230.32129846354214664No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTACCCAGTGGTGTG5190.31884111391658526No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCTACTTCAATGC5150.3163837642910239No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG5060.31085472763351085No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCACCACGTGCAGGA5060.31085472763351085No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC5040.3096260528207302No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCCCCAGTAGCTGG4970.3053256909759979No Hit
TGTAAAACGACGGCCAGTAGATCATCCAGCGCACACGGAGGAGACAGGCC4700.2887385810034587No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCCGGCAGCGACAT4660.2862812313778974No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTGACCCTTCCAGGA4580.28136653212677465No Hit
TGTAAAACGACGGCCAGTGCCTGTGCCTGACCACTACACACTGGTCATAG4440.2727658084373099No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGCTGAGGGAGGAT4130.25372134883920944No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCCAAAGTGCTGGG4110.25249267402642883No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTTGCGGCCCCTG4070.2500353244008674No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCAGTTTCCTGCC4050.24880664958808676No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGGATGCTGAGTGGGAG4040.2481923121816964No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGTGTGGCCTGAG4030.24757797477530608No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCAGCACCCGGCAGC3940.24204893811779304No Hit
TGTAAAACGACGGCCAGTCCAGGCATCTCCCAGCTGCGGTCATAGCTGTT3920.24082026330501238No Hit
TGTAAAACGACGGCCAGTCTTGCACTACATGGCCATCACCCCCTCTCCAC3800.23344821442832833No Hit
TGTAAAACGACGGCCAGTGACCTCATGGGGCGCTTCCCGGTCATAGCTGT3780.23221953961554764No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGCTAACGGGGTT3720.2285335151772056No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA3680.22607616555164428No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTCCATTTGGTGTG3670.22546182814525395No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC3640.22361881592608293No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGGTCCCCGGATGCC3620.22239014111330224No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGGCACGATTAGGG3550.2180897792685699No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTGTGAGGAGAATG3510.21563242964300852No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCCGCCCCAAGGAT3440.21133206779827615No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCAGCCCCTGCCA3330.2045743563279825No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCCCACAGACCCC3320.2039600189215921No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA3300.20273134410881147No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGGCCCTCGTTCCC3280.20150266929603078No Hit
TGTAAAACGACGGCCAGTAGATCATCCAGCGCACACGGGGAGACAGGCCC3250.19965965707685976No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGTGTGTGTTCGTGC3190.19597363263851772No Hit
TGTAAAACGACGGCCAGTCTCTCACCCACCTCCACCCACTGTTGAGCCCC3110.19105893338739502No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCCAGGGGGCGTCCCGG3060.18798724635544334No Hit
TGTAAAACGACGGCCAGTAGATCATCCAGCGCACACGGGAGACAGGCCCA3040.18675857154266265No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGAGGCTTTTTGTTCCA3030.18614423413627232No Hit
TGTAAAACGACGGCCAGTTCTTTGGAATTTAGTGTCTGAGCCTTGGATAC2920.17938652266597863No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGCTTGCAGCACA2880.17692917304041725No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGTGTAAAACTACG2880.17692917304041725No Hit
TGTAAAACGACGGCCAGTAGATCATCCAGCGCACACGGCAGGAGACAGGC2850.17508616082124623No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGGGTCAGTGGGAGTCT2790.17140013638290422No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT2760.1695571241637332No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAATTAGTAGT2740.16832844935095254No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG2720.16709977453817187No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGAGGATCCTCCC2630.16157073788065882No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC2610.16034206306787815No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCACCCCTGCCAA2570.15788471344231678No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTCCTCACTCGGAT2550.15665603862953612No Hit
TGTAAAACGACGGCCAGTTGACACAGCTGCTTCTGAGTACAAGTATGTAG2470.15174133937841341No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCAGGGAACAACT2390.14682664012729071No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCAGCCCCAAGGAT2390.14682664012729071No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTTGGACCCAGAG2380.14621230272090038No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGTGGGGTCACCCC2310.141911940876168No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC2200.1351542294058743No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG2190.13453989199948393No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGTGTTCGGAGGCA2170.13331121718670327No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGACACTGTGAGGCCC2130.13085386756114195No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGCATCCTGGGTGAC2100.12901085534197093No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGGGGGTGCCTCAA2080.12778218052919024No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGCTCTGGTG2000.12286748127806754No Hit
TGTAAAACGACGGCCAGTTGCCTTGAAGATTTTCTGTAGGATAAGTGAGC1920.11795278202694484No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTGGTGGAGTGTGT1910.1173384446205545No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGCACATTGGAGT1900.11672410721416417No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC1880.11549543240138348No Hit
TGTAAAACGACGGCCAGTAACAAATTCAGGTGAGATGCGCTCACATAGAA1880.11549543240138348No Hit
TGTAAAACGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT1870.11488109499499316No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCACATGTACTCT1860.1142667575886028No Hit
TGTAAAACGACGGCCAGTGCCACTTCTGAAGGAGGTTCCCCCAGTAGTGC1850.11365242018221249No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCTTTCTAACAGG1850.11365242018221249No Hit
TGTAAAACGACGGCCAGTCTCTCACCCACCTCCACCCAGTCACCCCTGCA1840.11303808277582214No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTGTGTTCACTGCA1820.11180940796304147No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCCCTCAGAGCTG1790.10996639574387046No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGGATGGTGTGGC1740.10689470871191875No Hit
TGTAAAACGACGGCCAGTCCTTGAAGGCTGGGCTTCTGTCTTCTGGCAGG1720.10566603389913808No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATTGAAAATCTGC1700.10443735908635741No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG1690.10382302167996707No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTTGACCATGAGTC1670.1025943468671864No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG1660.10198000946079605No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCGCTCCCCACCTCC1640.10075133464801539No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)