FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135857
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT45643.3594146786694834No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA40903.0105184127428104No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT37922.791170127413383No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC37722.776448765981878No Hit
CAGGAAACAGCTATGACCGGGGAGACTGGGCCATACGATAGGCTTAATCC29962.205259942439477No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT25721.8931670800915668No Hit
CAGGAAACAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC23771.7496338061343912No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT23441.7253435597724076No Hit
CAGGAAACAGCTATGACCCCCGTGACAGCTCCTGCAATTACAAAATGGCT22911.6863319519789188No Hit
CAGGAAACAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG21421.5766578093142054No Hit
CAGGAAACAGCTATGACCGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC21241.5634085840258507No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCATA19341.4235556504265514No Hit
CAGGAAACAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT17441.2837027168272521No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA17261.2704534915388974No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA16401.2071516373834252No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC15701.155626872373157No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC15031.1063103115776147No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGGACACTGCCCAC14621.076131520643029No Hit
CAGGAAACAGCTATGACCAGGACTTGCCCACTCTCCCAGTTACTTGGAAT14171.0430084574221425No Hit
CAGGAAACAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG13530.9959001008413259No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA13440.9892754881971486No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG12420.914196544896472No Hit
CAGGAAACAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG12400.9127244087533215No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA12300.9053637280375689No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC12000.8832816858903112No Hit
CAGGAAACAGCTATGACCTGGCATCCAGGGCCTATTTGGTTCCTATTGAG11720.8626717798862038No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGAGCCT11680.8597275075999028No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG11670.8589914395283276No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC10980.8082027425896346No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC10490.772135407082447No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC10310.7588861817940923No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT10060.7404844800047108No Hit
CAGGAAACAGCTATGACCCTGGGTGCCGGGTAGAGTGTATACAGGGCTCA9780.7198745740006036No Hit
CAGGAAACAGCTATGACCAGAGCTACTTCTGTGCCCACCATATTCCTCTA9140.672766217419787No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCCTGCCCAGGCC9090.6690858770619107No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT8270.6087282951927394No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG8050.5925347976180838No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCCATGCCTGCCGT7690.5660363470413744No Hit
CAGGAAACAGCTATGACCTCCAGCTGCTCCATCTTGTCTACCTCCCGGCC7490.5513149856098692No Hit
CAGGAAACAGCTATGACCGGAGAAGCACAGCTTCGGCTCTGGCCCTGGGG7220.5314411476773372No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACGGGGATGGCAGTG6810.5012623567427515No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGGCTCCCCACCT6420.47255570195131646No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGACACTGCCCACC6190.45562613630508547No Hit
CAGGAAACAGCTATGACCAGGTTGCTGGATTCAGTTTGCTGACCAACTTT6020.4431129790883061No Hit
CAGGAAACAGCTATGACCACCTGGCAGCCCCAAATCTGCACCTCCCGCGG5690.41882273272632253No Hit
CAGGAAACAGCTATGACCGCAGCAAGAGACCAATACCGCATTGTAGTCAA5430.3996849628653658No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT5400.39747675865064No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGGCACGGGAACA5140.37833898878968325No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT5090.37465864843180696No Hit
CAGGAAACAGCTATGACCTGGGTTGCCTGGGACTGAGAGGGTCCTGGGAC4760.35036840206982345No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTCACGCCTGTAA4660.3430077213540708No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT4660.3430077213540708No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGTGGTGGGCCACA4400.3238699514931141No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACACAGCTGGGAGGC4330.3187174749920873No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCCCGTTAGCTGC4140.3047321816321573No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCCCTTCCTTACCT3780.27823373105544796No Hit
CAGGAAACAGCTATGACCTCCAGTGGTGGGGCTCAACACGGGAAACCTCA3770.27749766298387274No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCCAAACCAG3760.27676159491229746No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTGATCCCCCAAAA3750.27602552684072224No Hit
CAGGAAACAGCTATGACCTCCAGTGGTGGGGCTCAACAAAGGCTCCGTGA3700.27234518648284595No Hit
CAGGAAACAGCTATGACCTTGGACAGCACCTTGAATGCTATCAGGGAAGC3660.2694009141965449No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG3640.2679287780533944No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCACAGGGACGCAG3610.2657205738386686No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCTGAATAGTTGT3520.25909596119449124No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCTATTTTCCAAAG3440.2532074166218892No Hit
CAGGAAACAGCTATGACCTCCTGCCCCACCCTGATACAATATAGTCTCCC3340.2458467359061366No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCAGCAGTAACAGG3250.23922212326195927No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG3100.22818110218833038No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAATAGTCT2990.22008435340100252No Hit
CAGGAAACAGCTATGACCGCAGCCACCCTGAACACACTTGGCTATCACCC2930.21566794497155095No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTTTCAGAGATGGT2930.21566794497155095No Hit
CAGGAAACAGCTATGACCTGACTGCAAACATAGAAGTGAGCAGAGCAAGA2790.20536299196949734No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACTAGAAGTAATTA2770.20389085582634683No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG2700.19873837932532No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCTAAACCAG2690.19800231125374473No Hit
CAGGAAACAGCTATGACCCTGGGCCCCAAAGCAAAGGACTCTTCCCAGTG2450.18033667753593852No Hit
CAGGAAACAGCTATGACCCCGTAGCCACGCCCCTATTGTCTGACCACGCT2380.17518420103491172No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCAGACAGGGACGC2370.17444813296333644No Hit
CAGGAAACAGCTATGACCCCCATCCACTAGTCGGGGAGTTACACAAACAG2350.17297599682018594No Hit
CAGGAAACAGCTATGACCGATGGCAGAATGGAAACATGGCAAAAAAGAGG2270.16708745224758387No Hit
CAGGAAACAGCTATGACCTCAGTTTCTTGTTTTCGTGATACTTATTCTTG2190.16119890767498177No Hit
CAGGAAACAGCTATGACCGATGGCAGAATGGAAACATGGCAAAAGAAGAG2180.16046283960340652No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACTCTTGGCCTGCT2160.15899070346025598No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTGATCTCCCCGGC2100.15457429503080444No Hit
CAGGAAACAGCTATGACCACCTGGCAGCCCCAAATCTGCACCTCCCGCAG2080.15310215888765394No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCGGTACTGGCCGT2070.15236609081607866No Hit
CAGGAAACAGCTATGACCTTGGACAGCACCTTGAATGCTTTCAGGGAAGC2000.14721361431505187No Hit
CAGGAAACAGCTATGACCGCTGAGGCAGGACAATCGCTTGAACTTGGGAG1840.13543652516984772No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGCAGGAAACAGCT1750.12881191252567037No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATATGAATTTCTGT1400.1030495300205363No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACTCGCCCCCTGCC1400.1030495300205363No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACTGCCTCCCGACT1390.10231346194896104No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)