FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84589
Sequences flagged as poor quality0
Sequence length300
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTTCATCTGTGCCATTTCCGTTTCCTGACAGATC15661.8513045431439077No Hit
TGTAAAACGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG14071.6633368404875337No Hit
TGTAAAACGACGGCCAGTTGCCCAACGTTGTAGTGTTGAGTGCATGGCAT13731.623142488976108No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC12391.4647294565487239No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC11941.4115310501365426No Hit
TGTAAAACGACGGCCAGTGACTGTAGGTTGGGCTCCCCCACAGGATCCTC11291.3346889075411696No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG11061.3074986109304993No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG9531.126624029129083No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC9281.0970693589000933No Hit
TGTAAAACGACGGCCAGTTGAAGAATGGTGGCTTGTTTCATGGTGGCCTT9061.0710612490985827No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT8591.0154984690680822No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGGTTGGAGGTG8320.9835794252207734No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC6890.8145267115109529No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT6780.8015226566101975No Hit
TGTAAAACGACGGCCAGTACCGACTGGAGAGGAAGGCACTGGTGGTAGCT6710.7932473489460804No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTTGTAAAACGAC6470.7648748655262504No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTCACCATTTGGTG6180.7305914480606225No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG5910.6986724042133138No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG5840.6903970965491968No Hit
TGTAAAACGACGGCCAGTTCATCTGTGCCATTTCCGTTTCCTGCACAGAT5680.6714821076026434No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC5650.6679355471751646No Hit
TGTAAAANGACGGCCAGTTCATCTGTGCCATTTCCGTTTCCTGACAGATC5440.6431096241828134No Hit
TGTAAAACGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT5320.6289233824728984No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG5160.6100083935263451No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTCCCGACGCCAGG5090.601733085862228No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG5060.5981865254347493No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA5000.5910934045797918No Hit
TGTAAAANGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG4680.553263426686685No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT4540.5367128113584508No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAGAGCAAAGTCC4530.5355306245492913No Hit
TGTAAAANGACGGCCAGTTGCCCAACGTTGTAGTGTTGAGTGCATGGCAT4400.5201621960302166No Hit
TGTAAAANGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC4220.4988828334653442No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC4180.4941540862287059No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC4160.4917897126103867No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAGAGCAAAGTC4080.48233221813711No Hit
TGTAAAACGACGGCCAGTCCTCTGGCATGCTGGTAGACTTGTGCCTGTTA4080.48233221813711No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCATAAACACAAA4030.47642128409131207No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC4020.4752390972821525No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC3980.4705103500455142No Hit
TGTAAAACGACGGCCAGTAGGTCATCCTCCTGCCTCGGTGAAAACGACGG3970.46932816323635457No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT3910.46223504238139707No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG3840.45395973471728No Hit
TGTAAAACGACGGCCAGTGCAGAAGATGGACTGTGGAGGGGGCTACATTA3700.43740911938904586No Hit
TGTAAAANGACGGCCAGTGACTGTAGGTTGGGCTCCCCCACAGGATCCTC3670.4338625589615671No Hit
TGTAAAANGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG3660.43268037215240757No Hit
TGTAAAACGACGGCCAGTACATGCGTGGCCCTGTCTACTCATCCCCCTCT3510.4149475700150138No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGGCCCTGCT3360.39721476787762No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCAGCCTGGGTGAT3300.39012164702266255No Hit
TGTAAAANGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC3190.3771175921219071No Hit
TGTAAAACGACGGCCAGTGGGGTGGGGAGTGAGATGGTCCTAGGAAGTAC3070.3629313504119921No Hit
TGTAAAANGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG3020.3570204163661942No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT2940.34756292189291754No Hit
TGTAAAANGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC2900.3428341746562792No Hit
TGTAAAANGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT2880.34046980103796004No Hit
TGTAAAANGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGGTTGGAGGTG2850.3369232406104813No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCTGGGCAGAAGG2810.33219449337384294No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTCAGGCGGGAGG2790.3298301197555238No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCAAGGGT2760.32628355932804504No Hit
TGTAAAACGACGGCCAGTGACTGTAGGTTGGGCTCCCCACACTCCAGGAT2720.3215548120914067No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACACTGCCGAGTG2710.3203726252822471No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCCT2700.31919043847308753No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTACCCAGTGGTGTG2680.31682606485476833No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTATTTCCCTACTT2680.31682606485476833No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTGGGGTTAGGCAT2660.3144616912364492No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCAGGGCAGAAGGG2650.3132795044272896No Hit
TGTAAAANGACGGCCAGTTGAAGAATGGTGGCTTGTTTCATGGTGGCCTT2630.31091513080897043No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC2530.29909326271737463No Hit
TGTAAAACGACGGCCAGTTGGATGGTGAGGGAAGGGCTAAGACAGGATTC2490.2943645154807363No Hit
TGTAAAACGACGGCCAGTACATGCGTGGCCCTGTCTACGGCCCCGCCCTC2490.2943645154807363No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC2470.29200014186241713No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG2400.2837248341983No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC2350.27781390015250207No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTGATAAGGAAGGA2340.2766317133433425No Hit
TGTAAAANGACGGCCAGTACCGACTGGAGAGGAAGGCACTGGTGGTAGCT2330.2754495265341829No Hit
TGTAAAANGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG2280.269538592488385No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGCCTGGGTGGGA2260.2671742188700658No Hit
TGTAAAANGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC2250.26599203206090627No Hit
TGTAAAANGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT2230.26362765844258707No Hit
TGTAAAANGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG2190.25889891120594877No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG2130.25180579035099127No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG2130.25180579035099127No Hit
TGTAAAACGACGGCCAGTGACTGTAGGTTGGGCTCCCCGACCCATGAGCT2000.23643736183191666No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG1990.2352551750227571No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCACTGCAGCACGC1980.2340729882135975No Hit
TGTAAAACGACGGCCAGTGCCCAAGGTTGGTGAGATGGGAACCACCCAGC1960.23170861459527836No Hit
TGTAAAANGACGGCCAGTTCCCACCACCATACCCCAACTTGTAAAACGAC1960.23170861459527836No Hit
TGTAAAANGACGGCCAGTGTGGTAACTGCTGCAGGGCTCACCATTTGGTG1930.2281620541677996No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCATCCTCCTCTTC1910.2257976805494804No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCGCAGCCCTCCTAC1900.22461549374032086No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGAGCCCCCTGCCG1890.22343330693116126No Hit
TGTAAAANGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG1880.22225112012200168No Hit
TGTAAAANGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG1840.21752237288536333No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT1760.20806487841208668No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG1750.20688269160292708No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTGACCCTTCCAGGA1750.20688269160292708No Hit
TGTAAAANGACGGCCAGTTCATCTGTGCCATTTCCGTTTCCTGCACAGAT1740.20570050479376753No Hit
TGTAAAANGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC1740.20570050479376753No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGAGGCTCCTTCC1710.20215394436628875No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC1700.20097175755712918No Hit
TGTAAAANGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT1680.19860738393881No Hit
TGTAAAANGACGGCCAGTCCTCTGGCATGCTGGTAGACTTGTGCCTGTTA1620.19151426308385253No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTGCCAGTACCGGG1620.19151426308385253No Hit
TGTAAAANGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC1620.19151426308385253No Hit
TGTAAAANGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA1590.18796770265637378No Hit
TGTAAAACGACGGCCAGTGGGGTGGGGAGTGAGATGGTGCCTGAAAGAAG1570.1856033290380546No Hit
TGTAAAANGACGGCCAGTACCGGGCCAGGTCAGTTACTCCCGACGCCAGG1540.18205676861057585No Hit
TGTAAAANGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAGAGCAAAGTC1490.17614583456477792No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAAGCCCCAGACCC1490.17614583456477792No Hit
TGTAAAACGACGGCCAGTACCGACTGGAGAGGAAGGCAGTGCCTTGCCAG1490.17614583456477792No Hit
TGTAAAANGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC1470.17378146094645877No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCGCTCCCACCCAG1470.17378146094645877No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCACCGAGGCAG1460.1725992741372992No Hit
TGTAAAACGACGGCCAGTTCCTTCTTGGGTGGGTATTTTGCAACTGGGTA1440.17023490051898002No Hit
TGTAAAACGACGGCCAGTACCGACTGGAGAGGAAGGCACCCGGGCTGCAG1430.16905271370982045No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC1420.16787052690066084No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTTGGGAAGGGA1420.16787052690066084No Hit
TGTAAAANGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCATAAACACAAA1370.16195959285486292No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTCTAACTGGATA1360.16077740604570334No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGTGGGGTCACCCC1340.15841303242738417No Hit
TGTAAAANGACGGCCAGTAGGTCATCCTCCTGCCTCGGTGAAAACGACGG1330.1572308456182246No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGCACATGGGT1310.15486647199990541No Hit
TGTAAAANGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAGAGCAAAGTCC1300.15368428519074584No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCGACCACACCACA1270.1501377247632671No Hit
TGTAAAANGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT1270.1501377247632671No Hit
TGTAAAANGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT1230.14540897752662876No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGTCCCAGCTACT1220.1442267907174692No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACCTGGCGCAGGC1210.1430446039083096No Hit
TGTAAAANGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC1200.14186241709915No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAGGAGAGCAAAG1170.13831585667167126No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGAAAGCCTCAGGCA1160.13713366986251166No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTCCATTTGGTGTG1140.1347692962441925No Hit
TGTAAAACGACGGCCAGTGACTGTAGGTTGGGCTCCCCGAGGGAGGATCC1140.1347692962441925No Hit
TGTAAAANGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC1130.1335871094350329No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCCCACAGACCCC1080.127676175389235No Hit
TGTAAAANGACGGCCAGTGACTGTAGGTTGGGCTCCCCACACTCCAGGAT1050.12412961496175626No Hit
TGTAAAANGACGGCCAGTACTGTCTGGAACCGCACTGCAGCCTGGGTGAT1030.1217652413434371No Hit
TGTAAAACGACGGCCAGTAGTGGAAGCTATTAGCACCAATGACAACTCCC1020.1205830545342775No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCTCCCCCTGCTG1010.11940086772511793No Hit
TGTAAAANGACGGCCAGTGCAGAAGATGGACTGTGGAGGGGGCTACATTA1010.11940086772511793No Hit
TGTAAAANGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCCT1010.11940086772511793No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG990.11703649410679876No Hit
TGTAAAANGACGGCCAGTGGGGTGGGGAGTGAGATGGTCCTAGGAAGTAC980.11585430729763918No Hit
TGTAAAACGACGGCCAGTAGGTCATCCTCCTGCCTCGGGTTCCGAGGTCA970.11467212048847958No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA970.11467212048847958No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCCGGAGTGCAATG970.11467212048847958No Hit
TGTAAAANGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG960.11348993367932No Hit
TGTAAAANGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC940.11112556006100084No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCACACCACAGCCC930.10994337325184125No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC930.10994337325184125No Hit
TGTAAAANGACGGCCAGTTCCCACCACCATACCCCAACTCAGGCGGGAGG930.10994337325184125No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCTGGAGTGCAGTG920.10876118644268166No Hit
TGTAAAANGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG920.10876118644268166No Hit
TGTAAAANGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCTGGGCAGAAGG920.10876118644268166No Hit
TGTAAAANGACGGCCAGTTGTGTGTTCCTGCTCCTGGCACTGCAGCACGC910.10757899963352209No Hit
TGTAAAACGACGGCCAGTAGAACAATGCGGCACTGAAGGAAGAGATGATT890.10521462601520291No Hit
TGTAAAANGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCAAGGGT880.10403243920604334No Hit
TGTAAAANGACGGCCAGTCCTGGGCGTCAGAGTGAGAACACTGCCGAGTG880.10403243920604334No Hit
TGTAAAANGACGGCCAGTCAGCAGCAACTCCCTGTGCTGATAAGGAAGGA880.10403243920604334No Hit
TGTAAAANGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC870.10285025239688377No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC860.10166806558772416No Hit
TGTAAAANGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC860.10166806558772416No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCCCTCGGAGCTG860.10166806558772416No Hit
TGTAAAANGACGGCCAGTGCCCAAGGTTGGTGAGATGGGAACCACCCAGC850.10048587877856459No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)