FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168645
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG113976.757982744818998No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA64913.8489133979661414No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC64623.831717513119274No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC62283.6929645112514455No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG56603.356162352871416No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC55923.315840967713244No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG55603.296866198227045No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT51593.059088618103116No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG50943.0205461175842747No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC42642.5283880340359928No Hit
TGTAAAACGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG31721.880874025319458No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG28861.7112870230365562No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC28181.6709656378783837No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC25711.5245041359067866No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC23581.3982033265142757No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA22251.3193394408372618No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT21871.2968069020724007No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC20561.2191289394882743No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG17061.0115923982329746No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA16360.9700850899819147No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCTGTAAAACGACG14930.8852915888404638No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCAAGGGT14450.8568294346111653No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT14420.8550505499718343No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA14230.8437842805894038No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC14090.8354828189391919No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC13900.8242165495567613No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC12720.7542470870764031No Hit
TGTAAAACGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG9820.5822882386077263No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG9590.5686501230395209No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG8250.4891932758160633No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG7710.4571733523081028No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCTGGAGGGCGGCCA7430.4405704290076789No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCTGGAGTGCAGTG7300.43286192890391056No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT6550.38838981292063207No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG6510.3860179667348572No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC6170.36585727415577096No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC4620.27394823445699545No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA3740.2217676183699487No Hit
TGTAAAACGACGGCTAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG3500.2075365412552996No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCTCAACAAGGAGACGATG3400.20160692579086248No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGTGCCTG2970.1761095792937828No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGGGGCCCGGATGT2950.17492365620089537No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG2880.1707729253757894No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG2850.16899404073645824No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGGG2790.16543627145779596No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCCACAAGGAGACGATG2710.16069257908624623No Hit
TGNAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAATAAGGAGACGATG2700.16009961753980254No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGGGAGGATCCTC2590.1535770405289217No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCACCTCAGCCTCC2590.1535770405289217No Hit
TGTAAAACGACGGCCAGTCACCAGCCATCTGGACCTGGTCATAGCTGTTT2550.15120519434314686No Hit
TGTAAAACGACGGCCAGTTCTTCACCTCCCAAAATGCTGGGATTACAGGC2490.14764742506448458No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGACCAGACG2450.1452755788787097No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGCCTCACAGGGTCATAGCTG2350.13934596341427258No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTACACTTAG2300.136381155682054No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCCCGTGTCC2220.13163746331050433No Hit
TGTAAAACGACGGCTAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG2200.1304515402176169No Hit
TGTAAAACGACGGCTAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC2100.12452192475317975No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCTGGAGGGCAGCCA2080.12333600166029232No Hit
TGTAAAACGACGGCTAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG2040.12096415547451747No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGCGCGGCGTGGGAGCCCC2020.11977823238163005No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCAATCATCTAGG1840.1091049245456432No Hit
TGTAAAACGACGGCTAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC1840.1091049245456432No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTTCGGCGTGGGAGCCCC1810.10732603990631208No Hit
TGNAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCTGAGTAGCCACAG1810.10732603990631208No Hit
TGTAAAACGACGGCTAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA1800.10673307835986835No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGCGCCTG1770.10495419372053723No Hit
TGTAAAACGACGGCCAGTGGGAGGCCATGTTGAACTTATTCAGAATGTAC1750.1037682706276498No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)