FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003245872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176045
Sequences flagged as poor quality0
Sequence length300
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCTGGGTGCTGGTG21391.2150302479479678No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC16830.9560055667584992No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT15150.8605754210571162No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC14780.8395580675395496No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT12610.7162941293419297No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC12150.6901644465903604No Hit
CAGGAAACAGCTATGACCGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC11780.6691470930727939No Hit
CAGGAAANAGCTATGACCACAGGAGACAGGATTGGGGCTGGGTGCTGGTG11620.6600585077679003No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA10750.6106393251725412No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA10590.6015507398676474No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC10210.5799653497685251No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCTGCAGTGCGGT9880.561220142577182No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCCGTCCTCGGAC9280.5271379476838308No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCTCTGCAGCTAGC9150.5197534721236048No Hit
CAGGAAANAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC9000.511232923400267No Hit
CAGGAAANAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC8140.4623817773864637No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACGGAGATCCAGCC8090.4595415944786845No Hit
CAGGAAACAGCTATGACCAGCGTCTCATTCCATAAAGGCCACCATGAAAC7890.44818086284756736No Hit
CAGGAAACAGCTATGACCGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG7820.44420460677667645No Hit
CAGGAAANAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT7530.4277315459115567No Hit
NAGGAAANAGCTATGACCACAGGAGACAGGATTGGGGCTGGGTGCTGGTG7390.4197790337697748No Hit
CAGGAAANAGCTATGACCCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT6940.3942173875997614No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG6920.39308131443664973No Hit
CAGGAAANAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC6810.38683291203953535No Hit
CAGGAAANAGCTATGACCGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC6710.3811525462239768No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT6460.3669516316850805No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT6330.35956715612485446No Hit
NAGGAAANAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC6020.341958022096623No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGACACACCACT5970.33911783918884375No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCCGTCCTCCCAGC5950.33798176602573204No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAACCATGTGCCC5930.33684569286262034No Hit
CAGGAAANAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA5670.3220767417421682No Hit
NAGGAAANAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC5670.3220767417421682No Hit
CAGGAAANAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC5590.3175324490897214No Hit
CAGGAAANAGCTATGACCCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA5440.3090119003663836No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA5390.30617171745860433No Hit
NAGGAAANAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT5240.29765116873526654No Hit
CAGGAAANAGCTATGACCTAGAGGACACCCAAGGCCACCCGTCCTCGGAC5230.2970831321537107No Hit
CAGGAAACAGCTATGACCGTCCTAGCACCGCAGCGAATGGGACCCGCAAG5030.2857224005225937No Hit
CAGGAAANAGCTATGACCCCTGGACGCAATCACCCAGGCTGCAGTGCGGT5030.2857224005225937No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCTGAGGCAGGAGA4920.2794739981254793No Hit
NAGGAAANAGCTATGACCCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT4880.2772018517992559No Hit
CAGGAAANAGCTATGACCGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG4850.2754977420545883No Hit
CAGGAAANAGCTATGACCTAGAGGACACCCAAGGCCACGGAGATCCAGCC4840.27492970547303247No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCCAAACCAG4800.27265755914680906No Hit
NAGGAAANAGCTATGACCGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC4690.2664091567496947No Hit
CAGGAAANAGCTATGACCAGCGTCTCATTCCATAAAGGCCACCATGAAAC4570.25959271777102444No Hit
CAGGAAANAGCTATGACCGCTGCTGACCCAACCCTACCTCTGCAGCTAGC4540.25788860802635694No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCAGAGAGTGATAT4540.25788860802635694No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAGGCCCCATCCGG4330.24595983981368397No Hit
NAGGAAANAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC4270.24255162032434888No Hit
CAGGAAANAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG4190.23800732767190208No Hit
CAGGAAACAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG4140.2351671447641228No Hit
NAGGAAANAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC4110.23346303501945526No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG4030.22891874236700846No Hit
NAGGAAANAGCTATGACCCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA4000.22721463262234087No Hit
NAGGAAANAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA3990.22664659604078502No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCGGGGATCCTCG3970.22551052287767331No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG3890.22096623022522652No Hit
NAGGAAANAGCTATGACCCCTGGACGCAATCACCCAGGCTGCAGTGCGGT3830.21755801073589137No Hit
CAGGAAACAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT3800.2158539009912238No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCTGCCCAACACCC3770.21414979124655628No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCAGGGCAGCGGT3760.21358175466500043No Hit
NAGGAAANAGCTATGACCTAGAGGACACCCAAGGCCACCCGTCCTCGGAC3650.20733335226788605No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCTAAACCAG3590.20392513277855095No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAGGCCGCAGCTCC3560.2022210230338834No Hit
CAGGAAANAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT3490.1982447669629924No Hit
CAGGAAACAGCTATGACCTCCTGCCCCACCCTGATACAGGTCACTGTGGA3450.195972620636769No Hit
CAGGAAANAGCTATGACCGCCTCTCAGGGCCTCACAGTGGACACACCACT3440.19540458405521316No Hit
NAGGAAANAGCTATGACCGCTGCTGACCCAACCCTACCTCTGCAGCTAGC3430.1948365474736573No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG3380.19199636456587804No Hit
CAGGAAANAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT3310.18802010849498707No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGTCTGGGTCCT3290.18688403533187536No Hit
CAGGAAACAGCTATGACCTGGAAACCCATGCCATGCACTCAACACTACAA3250.18461188900565198No Hit
NAGGAAANAGCTATGACCAGCGTCTCATTCCATAAAGGCCACCATGAAAC3190.18120366951631686No Hit
CAGGAAANAGCTATGACCCATGTGCCCCAGTAGAGAGCAACCATGTGCCC3160.1794995597716493No Hit
CAGGAAANAGCTATGACCCCACCCTCATGCCTAACCCCCGTCCTCCCAGC3160.1794995597716493No Hit
NAGGAAANAGCTATGACCGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG3120.1772274134454259No Hit
NAGGAAANAGCTATGACCTAGAGGACACCCAAGGCCACGGAGATCCAGCC3110.17665937686387004No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACGGCCCCTCTGCC3070.17438723053764663No Hit
CAGGAAACAGCTATGACCGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC3060.17381919395609077No Hit
CAGGAAACAGCTATGACCTGACAGCACAAGGAACACTGACCCGAGGCAGG3040.17268312079297907No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCCTCCCCACCTCC3030.1721150842114232No Hit
NAGGAAANAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG2970.1687068647220881No Hit
CAGGAAANAGCTATGACCGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA2960.16813882814053224No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT2880.16359453548808542No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG2860.16245846232497374No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2840.16132238916186203No Hit
CAGGAAACAGCTATGACCTGGGTTGCCTGGGACTGAGACGGCTGCGGAGG2810.15961827941719445No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCACAGGGACGCAG2790.15848220625408277No Hit
CAGGAAACAGCTATGACCACCTTTACCCAAATAGCTACCACCAGTGCCTT2760.1567780965094152No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT2760.1567780965094152No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGAAAAGTTTCAGG2640.14996165753074497No Hit
CAGGAAANAGCTATGACCAATGCAAGGGACCACCAGGCTGAGGCAGGAGA2630.14939362094918912No Hit
CAGGAAANAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG2560.14541736487829815No Hit
CAGGAAANAGCTATGACCGTCCTAGCACCGCAGCGAATGGGACCCGCAAG2540.14428129171518644No Hit
CAGGAAACAGCTATGACCATCGCACCACTGCACTCCAGTGGCGCTCCATA2480.14087307222585133No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG2470.1403050356442955No Hit
NAGGAAANAGCTATGACCGCCTCTCAGGGCCTCACAGTGGACACACCACT2460.13973699906273965No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG2440.13860092589962794No Hit
CAGGAAACAGCTATGACCATCGCACCACTGCACTCCAGCGCTCCATACAC2440.13860092589962794No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAACGCAGCTCCCCA2440.13860092589962794No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAAGGGGACATGGCC2390.13576074299184868No Hit
CAGGAAACAGCTATGACCGGGTCCCACCCATGCTGAAATGTGATCCTCAA2340.1329205600840694No Hit
NAGGAAANAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT2340.1329205600840694No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTATTGCAGCCTGT2320.1317844869209577No Hit
NAGGAAANAGNTATGANCACAGGAGACAGGATTGGGGCTGGGTGCTGGTG2290.13008037717629015No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCTCTCCACGTGC2290.13008037717629015No Hit
CAGGAAANAGCTATGACCGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG2280.1295123405947343No Hit
CAGGAAANAGCTATGACCTGTTTTGCCCCTGAAATGAATATCCAAACCAG2280.1295123405947343No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCAAACTTGG2260.12837626743162261No Hit
NAGGAAANAGCTATGACCGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA2230.12667215768695503No Hit
CAGGAAANAGCTATGACCAATGCAAGGGACCACCAGGCAGAGAGTGATAT2230.12667215768695503No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG2220.12610412110539918No Hit
CAGGAAANAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT2200.12496804794228748No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCAGGCAC2190.12440001136073163No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAACAGGCCACCGCA2180.12383197477917579No Hit
CAGGAAANAGCTATGACCGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC2140.12155982845295236No Hit
CAGGAAANAGCTATGACCTCCTGGAACCTGCCTCCGAAGGCCCCATCCGG2120.12042375528984067No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT2120.12042375528984067No Hit
NAGGAAANAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT2110.11985571870828482No Hit
CAGGAAANAGCTATGACCTAGAGGACACCCAAGGCCACCAGGGCAGCGGT2100.11928768212672897No Hit
NAGGAAANAGCTATGACCGTCCTAGCACCGCAGCGAATGGGACCCGCAAG2100.11928768212672897No Hit
CAGGAAANAGCTATGACCAACACGGCAGTCAGAGGTGCTGCCCAACACCC2090.11871964554517311No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAATAAATAGGCAGC2090.11871964554517311No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCAGGCCCTGGCGT2080.11815160896361725No Hit
CAGGAAANAGCTATGACCTGTTTTGCCCCTGAAATGAATATCTAAACCAG2070.1175835723820614No Hit
NAGGAAANAGCTATGACCCCACCCTCATGCCTAACCCCCGTCCTCCCAGC2050.1164474992189497No Hit
NAGGAAANAGNTATGANCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC2040.11587946263739385No Hit
NAGGAAANAGCTATGACCAATGCAAGGGACCACCAGGCTGAGGCAGGAGA2000.11360731631117044No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT1970.11190320656650288No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTAGTCTC1970.11190320656650288No Hit
CAGGAAANAGCTATGACCGCCTCTCAGGGCCTCACAGTGGTCTGGGTCCT1940.11019909682183533No Hit
CAGGAAANAGCTATGACCCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG1900.1079269504956119No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC1900.1079269504956119No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCTTCCCATCTCA1900.1079269504956119No Hit
NAGGAAANAGCTATGACCCATGTGCCCCAGTAGAGAGCAACCATGTGCCC1900.1079269504956119No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTCCTCCCTTTGG1890.10735891391405607No Hit
CAGGAAANAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG1890.10735891391405607No Hit
CAGGAAANAGCTATGACCTCCTGGAACCTGCCTCCGAAGGCCGCAGCTCC1890.10735891391405607No Hit
CAGGAAANAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG1880.10679087733250021No Hit
CAGGAAANAGCTATGACCTCCTGCCCCACCCTGATACAGGTCACTGTGGA1850.10508676758783267No Hit
CAGGAAANAGCTATGACCTAGAGGACACCCAAGGCCACCGGGGATCCTCG1850.10508676758783267No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGTGCTGCCTCTCCA1840.10451873100627679No Hit
CAGGAAACAGCTATGACCGCTAATGCTTACTCTTTGCGTGAGTACTGGTT1830.10395069442472095No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCCTGGGTGTCTG1820.1033826578431651No Hit
CAGGAAANAGCTATGACCGGAAGGCAGGTGGTGGGAACGGCCCCTCTGCC1800.10224658468005339No Hit
CAGGAAANAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1790.10167854809849755No Hit
NAGGAAANAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG1780.1011105115169417No Hit
NAGGAAANAGNTATGANCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1780.1011105115169417No Hit
CAGGAAACAGCTATGACCTGGGTTATGGCGTGGGATGCAGGAAACAGCTA1780.1011105115169417No Hit
CAGGAAANAGCTATGACCTGGGTTGCCTGGGACTGAGACGGCTGCGGAGG1770.10054247493538584No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)