FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003245873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176276
Sequences flagged as poor quality0
Sequence length300
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA65013.6879665978352127No Hit
TGTAAAACGACGGCCAGTCAGCCAGTAGCAACAGCAGCTACTGATGTAAG48132.7303773627720167No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA46182.6197553836029863No Hit
TGTAAAACGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTCTGGCATGG39492.240236901223082No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG38382.177267466926865No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC37802.144364519276589No Hit
TGTAAAACGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT35682.0240985726928225No Hit
TGTAAAACGACGGCCAGTACTAAACTGTAGTGAACAGAGACATGCCCAGG34661.9662347682044068No Hit
TGTAAAACGACGGCCAGTCCAGAGATCAATAATAGCTCAGGGGAGATGGA32731.85674737343711No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC30931.754634777281082No Hit
TGTAAAACGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT28461.614513603666977No Hit
TGTAAAACGACGGCCAGTCCCAGCACAACTTCCCCCATGGCGTGGTGTCC26111.4811999364632735No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC25181.428441761782659No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC23441.3297329188318319No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC22321.2661961923347478No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT20211.1464975379518483No Hit
TGTAAAACGACGGCCAGTTGAGAAGCTGAGGCTTAAAGAGGGATCCCTTG19371.0988449930790352No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT18161.0302026367741495No Hit
TGTAAAACGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG17981.019991377158547No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCAGTTTCCTGCC17610.9990015657264744No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG14710.8344868274750958No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT14570.8265447366629604No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC13990.7936417890126847No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC13850.7856996982005491No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTGGGGTGCT13660.7749211463840795No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCATGACAAGGCT13620.7726519775806122No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT13560.7692482243754113No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG13530.7675463477728108No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC13080.7420181987338038No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT12970.7357779845242688No Hit
TGTAAAACGACGGCCAGTTCTTTGGCTCTACCTAAAAGTTGGTCATAGCT12810.7267013093103996No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT12800.7261340171095327No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGACGGGTGGCC12640.7170573418956636No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGGCAGCACCTCT12560.712519004288729No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCCAAGAGGTGGGG12380.7023077446731262No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT12350.7006058680705257No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG11980.6796160566384534No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCGTGACAAGGCT11760.6671356282193832No Hit
TGTAAAACGACGGCCAGTTCATAGCTGTGAAGAGGGATACCAAGGAAAGT11740.6660010438176496No Hit
TGTAAAACGACGGCCAGTAAGGGTGGGTCATGGGGTGAGGGTGGGTCGCG11650.6608954140098482No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGACGGCACTGGTG11430.6484149855907781No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG10980.6228868365517711No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC10980.6228868365517711No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCACCCCTGCCAA10960.6217522521500375No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC10160.5763688760806917No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC10060.5706959540720234No Hit
TGTAAAACGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT10040.5695613696702898No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGATCCCCGGTG9930.5633211554607548No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGTCATAGCTGTT9550.5417640518278154No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG9530.5406294674260818No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCCGGCCCCAAGGA9510.5394948830243482No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCGAGCAGGTGGC9510.5394948830243482No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCAAGAGGTGGGGT9420.5343892532165468No Hit
TGTAAAACGACGGCCAGTAAGAGGTGAGCGAGGTCTGCGCCACGTGATCC9240.524177993600944No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC9210.5224761169983435No Hit
TGTAAAACGACGGCCAGTACATGCGTGGCCCTGTCTACTCATCCCCCTCT9070.514534026186208No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTTGGCTCACT8970.5088611041775398No Hit
TGTAAAACGACGGCCAGTTGGAGTGCCTGGGTTAGAACCCTGCTGGTACC8750.49638067575846967No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCCCCAGTAGCTGG8710.4941115069550024No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGCCTGGGTGGGA8660.49127504595066823No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACAGCCTGGGTGG8340.47312169552292993No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC8070.4578048060995258No Hit
TGTAAAACGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGGGACACGGA7940.45043000748825707No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC7900.44816083868478973No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCCACGCTGGGAAT7860.44589166988132245No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC7850.44532437768045563No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGCCCCAATCCTGTCTC7490.42490185844925005No Hit
TGTAAAACGACGGCCAGTGCCCTGAACCGGTGCCAAATGGAGTGAAGAAA7400.4197962286414486No Hit
TGTAAAACGACGGCCAGTTTGCTGCTGGGCTTCCTCTGCCCTGAGGGCTT6970.39540266400417523No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCTCCTGCTCAGCCTT6840.38802786539290657No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGCCTTCGGAGGC6540.3710090993669019No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA6240.35399033334089725No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTACCCAGTGGTGTG5920.33583698291315894No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG5460.30974154167328505No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG5380.3052032040663505No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC5270.29896298985681546No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTGACCCTTCCAGGA5260.29839569765594864No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCCCTCGGAGCTG5060.2870498536386122No Hit
TGTAAAACGACGGCCAGTAGGTCATCCTCCTGCCTCGGCCTCCCAAAGTG5010.2842133926342781No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGGCTGTCATCTCT4720.26776191880914024No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCCCCTGTGCCTC4680.2654927500056729No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCGCCTCGGCCTGC4510.25584878259093696No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCCCATCCCTCAG4200.23826272436406545No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGTGAACGAAG4180.23712813996233179No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCCGAGTAGCTGAG4170.23656084776146497No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCATCAGGTTCCC4130.23429167895799768No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGGAGCTGATGGGA4020.22805146474846263No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCAGAGGGGTCGGGG4020.22805146474846263No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT3950.22408041934239487No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTTGTAAAACGAC3890.22067666613719397No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTGGGGTTAGGCAT3840.21784020513285984No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG3820.2167056207311262No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGTTCGGAGGCAGG3780.2144364519276589No Hit
TGTAAAACGACGGCCAGTTTGCTGCTGGGCTTCCTCTGCACTTGAAAGGA3760.21330186752592525No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCTGCAGACAGGAT3640.20649436111552338No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGGCTCCAGGATCG3600.2042251923120561No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC3380.191744763892986No Hit
TGTAAAACGACGGCCAGTCCACCTGCTTCGCACCATCTCGGCCCAGCCCA3380.191744763892986No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAAACCCCAGACCC3200.18153350427738318No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGTCATAGCTGTC3120.17699516667044862No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGTGGCCTGTTCG3070.17415870566611452No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTGTACCGGGGCCA2990.1696203680591799No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGCAGGAAGCACCT2980.16905307585831308No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGCCGGTGCCCAT2940.1667839070548458No Hit
TGTAAAACGACGGCCAGTTCCCACCACCTGCCTTCCGGTCATAGCTGTTT2840.16111098504617757No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCCCACAGACCCC2830.16054369284531075No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGGCGGCACCTCT2590.14692868002450704No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG2540.1440922190201729No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAAGCCCCAGACCC2500.1418230502167056No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGCATTTCCTTGGC2500.1418230502167056No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCGCCTCCTGCTCAGCC2430.13785200481063786No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGGGGTCAT2360.13388095940457012No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGAAGCCCAGTCAGG2360.13388095940457012No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCCGTGGG2320.1316117906011028No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGGAGAGACTCCGC2280.12934262179763553No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGCCAT2270.12877532959676868No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGGCACTCTTGGTG2220.12593886859243458No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCACCGAGGCAG2200.12480428419070094No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC2120.12026594658376635No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGGGGGTCGGTAAT2070.11742948557943225No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCGTGGGGCACTCTTGGTG2060.11686219337856543No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGAGGATCCTCCCT2060.11686219337856543No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGTGCCGCTCTCCG2040.11572760897683178No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGCCCACTGCCTAT1830.10381447275862853No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)