FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120318
Sequences flagged as poor quality0
Sequence length300
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC31022.578167855183763No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC25852.1484732126531356No Hit
CAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT24872.0670223906647385No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA17171.4270516464701872No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG16411.3638857028873486No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG16261.3514187403381042No Hit
CAGGAAANAGCTATGANNTGGCCCAGCCTATGCCTTTCTAATTGCTCTAG15731.30736880599744No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA14961.243371731577985No Hit
CAGGAAANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC11850.9848900413903158No Hit
CAGGAAANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC11070.9200618361342443No Hit
CAGGAAANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT9980.8294685749430675No Hit
CAGGAAANAGCTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG8650.7189281736730996No Hit
CAGGAAANAGCTATGANNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG8260.6865140710450639No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA8180.6798650243521335No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG7590.6308283049917718No Hit
CAGGAAANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG6820.5668312305723168No Hit
CAGGAAANAGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT6560.5452218288202928No Hit
CAGGAAANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG6550.5443906979836766No Hit
CAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA6520.5418973054738276No Hit
CAGGAAANAGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA6290.5227812962316528No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA6150.5111454645190245No Hit
CAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA6140.5103143336824083No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT6020.5003407636430127No Hit
CAGGAAANAGCTATGANNTGCTGGGAGAGGGGGATGAGCGGCGCCGCCAT5970.49618510945993116No Hit
CAGGAAANNGCTATGANNTGGCCCAGCCTATGCCTTTCTAATTGCTCTAG5880.48870493193038445No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC5790.4812247544008378No Hit
CAGGAAANAGCTATGANNGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT5430.45130404428265103No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG4690.3898003623730448No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA3990.3316212038099038No Hit
CAGGAAANNGCTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG3590.29837597034525176No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA3520.2925580544889376No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT3380.28092222277630946No Hit
CAGGAAANAGCTATGANNAGATCCTGTGAAGTGGGAGGGTTTTCAAAGTC3130.26014395186090195No Hit
CAGGAAANNGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA3070.25515716684120415No Hit
NAGGANANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC3040.2526637743313553No Hit
CAGGAAANNGCTATGANNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG2980.24767698931165744No Hit
CAGGAAANAGCTATGANNGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA2890.24019681178211075No Hit
CAGGANANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2880.23936568094549446No Hit
NAGGANANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2830.23521002676241293No Hit
CAGGAAANNGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA2800.23271663425256406No Hit
NAGGAAANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2800.23271663425256406No Hit
CAGGAAANAGCTATGANNGCCCTGGCCACTGTGGTTAATGAAACTTGGTT2720.22606758755963366No Hit
NAGGAAANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2690.22357419504978474No Hit
CAGGAAANNGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2670.22191193337655213No Hit
CAGGAAANAGCTATGANNAAGTTGGTCAGCCCTGGTGACTCATTGGATAT2640.21941854086670326No Hit
CAGGAAANAGCTATGANNCAGAAGGCTGAGCAGGAGGCTGGGAAAAAGGG2630.21858741003008691No Hit
CAGGANANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2620.21775627919347063No Hit
CAGGANANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2460.20445818580760983No Hit
CAGGAAANAGCTATGANNGCTGCTGACCCAACCCTACCCTGTCCAGCCCC2460.20445818580760983No Hit
CAGGAAANAGCTATGANNCCTGGACGCAATCACCCAGGCCAGCTAAGTGA2440.20279592413437725No Hit
CAGGAAANAGCTATGANNAGGCTTTGTGTGTGGGGACTTGTAATCCCATC2400.19947140078791203No Hit
CAGGAAANNGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA2380.19780913911467943No Hit
NAGGAAANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2360.19614687744144685No Hit
NAGGANANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2330.19365348493159792No Hit
CAGGAAANNGCTATGANNTGCTGGGAGAGGGGGATGAGCGGCGCCGCCAT2250.18700443823866753No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA2230.18534217656543492No Hit
CAGGAAANAGCTATGANNGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG2220.18451104572881863No Hit
CAGGAAANAGCTATGANNTCCAGTGGTGGGGCTCAACAAAGGCTCCGTGA2190.18201765321896973No Hit
CAGGAAANNGCTATGANNGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT2140.17786199903588823No Hit
CAGGAAANAGCTATGANNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT2110.1753686065260393No Hit
CAGGAAANAGCTATGANNTGGGTTGCCTGGGACTGAGAGGGTCCTGGGAC2070.17204408317957415No Hit
CAGGAAANNGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT2040.16955069066972522No Hit
CAGGAAANAGCTATGANNGCCCCTACTCCAAAAGGCGGATGAATGGGGAA2020.16788842899649262No Hit
CAGGANANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1950.16207051314017853No Hit
NAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA1930.16040825146694593No Hit
CAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1920.1595771206303296No Hit
CAGGAAANAGCTATGANNTCCAGTGGTGGGGCTCAACAAAGGCTCTGTGA1910.15874598979371332No Hit
CAGGAAANAGCTATGANNTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC1880.15625259728386443No Hit
CAGGAAACAGCTATGANCACAGTGTCCCCATCAGGCTCCGGGACAGCATC1860.15459033561063182No Hit
CAGGAAACNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1860.15459033561063182No Hit
CAGGAANNNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1860.15459033561063182No Hit
CAGGAAANAGCTATGANNGCAAAACACCTTAACACTCGGCTTCTATTTGC1840.1529280739373992No Hit
NAGGAAANAGCTATGANCACAGTGTCCCCATCAGGCTCCGGGACAGCATC1840.1529280739373992No Hit
CAGGAAANNGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT1840.1529280739373992No Hit
NAGGANANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA1820.15126581226416663No Hit
NAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1820.15126581226416663No Hit
CAGGAAACAGCNATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1790.14877241975431774No Hit
CAGGAANNNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1740.1446167655712362No Hit
NAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA1740.1446167655712362No Hit
NAGGANANNGCTATGANNTGGCCCAGCCTATGCCTTTCTAATTGCTCTAG1710.14212337306138734No Hit
NAGGANANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1700.14129224222477102No Hit
CAGGAAANNGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA1700.14129224222477102No Hit
CAGGAAANNGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1700.14129224222477102No Hit
CAGGANANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA1690.14046111138815473No Hit
CAGGAAANAGCTATGANNTGGGTTATGGCGTGGGATGCCCTGCAACCCCC1690.14046111138815473No Hit
NAGGAAANNGCTATGANNTGGCCCAGCCTATGCCTTTCTAATTGCTCTAG1680.1396299805515384No Hit
CAGGAAACAGCTATGANCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1660.1379677188783058No Hit
CAGGAAANAGCTATGANNTCTGACTGTCCGTGATCCCACACAATCACCCT1650.13713658804168952No Hit
CAGGAAACAGCTATGANCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1630.1354743263684569No Hit
CAGGANANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1620.13464319553184062No Hit
NAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1600.132980933858608No Hit
NAGGANANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1600.132980933858608No Hit
CAGGAAANAGCTATGANNCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT1580.1313186721853754No Hit
CAGGAAACNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1560.12965641051214283No Hit
NAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1560.12965641051214283No Hit
CAGGAAACNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1550.1288252796755265No Hit
NAGGANANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA1510.12550075632906132No Hit
CAGGANANNGCTATGANNTGGCCCAGCCTATGCCTTTCTAATTGCTCTAG1490.12383849465582872No Hit
CAGGANANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA1480.12300736381921243No Hit
CAGGAAANNGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1480.12300736381921243No Hit
CAGGAANNNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1470.12217623298259611No Hit
NAGGAAANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1450.12051397130936352No Hit
NAGGAAANAGCTATGANCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1450.12051397130936352No Hit
NAGGAAANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1420.11802057879951462No Hit
NAGGAAANAGCTATGANCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1400.11635831712628203No Hit
CAGGAAANNGCTATGANNAGATCCTGTGAAGTGGGAGGGTTTTCAAAGTC1380.11469605545304942No Hit
CAGGAAANAGCTATGANNACAAACGCAAATGGCTTTTAGCATTTCACAGG1350.11220266294320051No Hit
CAGGAAANAGCTATGANNTGTTGTCTTTCAGGGGCCGCCATGCCTGCCGT1300.10804700876011902No Hit
CAGGAAANNGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT1290.1072158779235027No Hit
CAGGAAACNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1270.10555361625027011No Hit
CAGGAAANNGCTATGANNCAGAAGGCTGAGCAGGAGGCTGGGAAAAAGGG1240.10306022374042122No Hit
CAGGAAACAGCNATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1220.10139796206718862No Hit
CAGGAAANAGCTATGANNGCTACGGGCAATCACTCTCCTACTCCCAACCA1210.1005668312305723No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)