FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144398
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAANGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGCGGTCATA36422.5221955982769844No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGCGGTCATA35842.482028836964501No Hit
TGTAAAANGACGGCCAGTAACAAATTCAGGTGAGATGCGCTCATATAGAA34902.4169309824235796No Hit
TGTAAAACGACGGCCAGTAACAAATTCAGGTGAGATGCGCTCATATAGAA30912.140611365808391No Hit
TGTAAAANGACGGCCAGTGGGGAGAGGAAGGCCAGGAGGAGTGGTCATAG22541.5609634482472057No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGGAGGAGTGGTCATAG22051.5270294602418317No Hit
TGTAAAANGACGGCCAGTACACTCCCTCCACCTCCCATCCCAGAAAAAGT20081.390600977852879No Hit
TGTAAAANGACGGCCAGTGGAGCGACTTGACAATGCCCCTCACACCCCTT19111.3234255322095874No Hit
TGTAAAANGACGGCCAGTGGCACAGCCAAGAGTTGGGAGTGGTCATAGCT18791.3012645604509758No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGTGGTCATAGCT17861.2368592362775108No Hit
TGTAAAACGACGGCCAGTACACTCCCTCCACCTCCCATCCCAGAAAAAGT17291.197385005332484No Hit
TGTAAAACGACGGCCAGTGGAGCGACTTGACAATGCCCCTCACACCCCTT17071.1821493372484384No Hit
TGTAAAANGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC16641.1523705314478039No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC16381.134364741893932No Hit
TGTAAAANGACGGCCAGTTGGATGGTGAGGGAAGGGGAAGACTGGCAGGT14210.984085652155847No Hit
TGTAAAACGACGGCCAGTTGGATGGTGAGGGAAGGGGAAGACTGGCAGGT14010.9702350448067147No Hit
TGTAAAANGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC13930.9646948018670619No Hit
TGTAAAANGACGGCCAGTGCCCAAGGTTGGTGAGATGCGCTCATATAGAA12520.8670480200556795No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC12370.8566600645438303No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT12290.8511198216041774No Hit
TGTAAAACGACGGCCAGTGCCCAAGGTTGGTGAGATGCGCTCATATAGAA12110.8386542749899584No Hit
TGTAAAANGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG12050.8344990927852186No Hit
TGTAAAANGACGGCCAGTACACATTCTTGTAAAGCATGGGATTGCTGAAT11700.8102605299242371No Hit
TGTAAAANGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT11560.8005651047798447No Hit
TGTAAAANGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC11460.7936398011052784No Hit
TGTAAAACGACGGCCAGTGCCCAAGGTTGGTGAGATGGGCTCATATAGAA11430.7915622100029086No Hit
TGTAAAANGACGGCCAGTGCCCAAGGTTGGTGAGATGGGCTCATATAGAA11120.7700937686117537No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC11030.7638609953046441No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG10660.7382373717087495No Hit
TGTAAAACGACGGCCAGTACACATTCTTGTAAAGCATGGGATTGCTGAAT10000.6925303674566129No Hit
TGTAAAANGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC9390.6502860150417596No Hit
TGTAAAANGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG9260.6412831202648236No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC9250.640590589897367No Hit
TGTAAAANGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG8970.6211997396085818No Hit
TGTAAAANGACGGCCAGTCGGGTGGGTATTGGTCTCTTGCTGCGGTCATA8570.5934985249103173No Hit
TGTAAAANGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCCGTGGG8400.5817255086635549No Hit
TGTAAAANGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG8320.5761852657239019No Hit
TGTAAAANGACGGCCAGTAGTACTCCCTCCTGCCTGTGGTCATAGCTGTT8090.5602570672723999No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG8080.5595645369049433No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTATTGGTCTCTTGCTGCGGTCATA7970.5519467028629205No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG7750.536711034778875No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGTCATAGCTGTT7710.5339409133090487No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCCGTGGG7580.5249380185321126No Hit
TGTAAAANGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA7210.49931439493621793No Hit
TGTAAAANGACGGCCAGTGTGGTAACTGCTGCAGGGCTGAGGGTCATAGC7200.49862186456876134No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTGAGGGTCATAGC6930.47992354464743275No Hit
TGTAAAANGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG6840.4736907713403233No Hit
TGTAAAANGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG6830.47299824097286663No Hit
TGTAAAANGACGGCCAGTACACTCCCCTCACCCCACCCTAGGGTCATAGC6660.4612252247261042No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG6520.4515297995817117No Hit
TGTAAAACGACGGCCAGTACACTCCCCTCACCCCACCCTAGGGTCATAGC6480.44875967811188516No Hit
TGTAAAANGACGGCCAGTGAGACAGGCTTCTGGGCTGTCCACAACTCTCT6310.43698666186512275No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA6230.4314464189254699No Hit
TGTAAAANGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT6040.4182883419437942No Hit
TGTAAAANGACGGCCAGTTCCAAGGTGCTGTTTCAAACTCCAGGTCATAG5850.40513026496211857No Hit
TGTAAAANGACGGCCAGTGTTCTACCGTCAGTCCTGGCATTATTTCTCTC5790.4009750827573789No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG5790.4009750827573789No Hit
TGTAAAACGACGGCCAGTTCCAAGGTGCTGTTTCAAACTCCAGGTCATAG5640.3905871272455297No Hit
TGTAAAACGACGGCCAGTGAGACAGGCTTCTGGGCTGTCCACAACTCTCT5590.3871244754082466No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG5590.3871244754082466No Hit
TGTAAAANGACGGCCAGTTCCATTACTCTACACAAGCAGGGTACACTTAG5380.3725813376916578No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT5300.36704109475200486No Hit
TGTAAAANGACGGCCAGTTCACAGTGCTGTAGGGGCCTACACGTTCCAGG5030.3483427748306763No Hit
TGTAAAACGACGGCCAGTGTTCTACCGTCAGTCCTGGCATTATTTCTCTC4990.34557265336084986No Hit
TGTAAAANGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCAGTGGG4940.3421100015235668No Hit
TGTAAAACGACGGCCAGTCCCTCCAGCTGCTCTCTGCTGGAGCGGTCATA4880.33795481931882715No Hit
TGTAAAANGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT4850.33587722821645727No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTACACTTAG4630.3206415601324118No Hit
TGTAAAACGACGGCCAGTGGGCAGGAGAGGCAGCTAACGGGTCATAGCTG4590.3178714386625854No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCAGTGGG4570.31648637792767215No Hit
TGTAAAANGACGGCCAGTCGCAATGTGGCCTTGGGTGTCCTCTAGGTCAT4320.2991731187412568No Hit
TGTAAAANGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT4300.29778805800634356No Hit
TGTAAAANGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT4240.2936328758016039No Hit
TGTAAAANGACGGCCAGTCCCTCCAGCTGCTCTCTGCTGGAGCGGTCATA4240.2936328758016039No Hit
TGTAAAANGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC4220.2922478150666907No Hit
TGTAAAANGACGGCCAGTTCCTTCCTGGGTGGGACGTTGCTACCGGTCAT4220.2922478150666907No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT4200.29086275433177744No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTTGGGTGTCCTCTAGGTCAT4020.2783972077175584No Hit
TGTAAAANGACGGCCAGTGGGCAGGAGAGGCAGCTAACGGGTCATAGCTG4000.2770121469826452No Hit
TGTAAAANGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG3990.2763196166151886No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT3990.2763196166151886No Hit
TGTAAAACGACGGCCAGTTCCTTCCTGGGTGGGACGTTGCTACCGGTCAT3940.2728569647779055No Hit
TGTAAAACGACGGCCAGTTCACAGTGCTGTAGGGGCCTACACGTTCCAGG3850.26662419147079597No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC3810.26385407000096955No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG3740.25900635742877326No Hit
TGTAAAANGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG3740.25900635742877326No Hit
TGTAAAANGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC3620.25069599301929385No Hit
TGTAAAANGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT3440.23823044640507487No Hit
TGTAAAACGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG3410.23615285530270502No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT3380.23407526420033517No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC3230.223687308688486No Hit
TGTAAAANGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA3120.21606947464646326No Hit
TGTAAAANGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCATTTATT3100.21468441391155002No Hit
TGTAAAANGACGGCCAGTGACTGTAGGTTGGGCTCCCCATGTGCTGTGCC3090.2139918835440934No Hit
TGTAAAANGACGGCCAGTGCCCAAGGTTGGTGAGATGGGAACCACCCAGC2950.20429645839970084No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA2760.1911383814180252No Hit
TGTAAAANGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA2720.18836825994819872No Hit
TGTAAAACGACGGCCAGTGACTGTAGGTTGGGCTCCCCATGTGCTGTGCC2710.18767572958074213No Hit
TGTAAAACGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCATTTATT2620.1814429562736326No Hit
TGTAAAANGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG2610.180750425906176No Hit
TGTAAAACGACGGCCAGTGCCCAAGGTTGGTGAGATGGGAACCACCCAGC2460.1703624703943268No Hit
TGTAAAANGACGGCCAGTTCCCACCACCATACCCCAACTTAGTATTTGAA2430.16828487929195696No Hit
TGTAAAACGACGGCCAGTCCCTCCAGCTGCTCTCTGCCCAGCGGCAGCAG2390.16551475782213051No Hit
TGTAAAANGACGGCCAGTCCCTCCAGCTGCTCTCTGCCCAGCGGCAGCAG2390.16551475782213051No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT2370.16412969708721728No Hit
TGTAAAANGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA2310.1599745148824776No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG2270.15720439341265113No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGAGCTGAGGGTCATAGC2260.15651186304519454No Hit
TGTAAAANGACGGCCAGTGTGGTAACTGCTGCAGAGCTGAGGGTCATAGC2220.15374174157536807No Hit
TGTAAAANGACGGCCAGTTCCAAGGTTCTGTTTCAAACTCCAGGTCATAG2220.15374174157536807No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA2210.15304921120791148No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTTTCAAACTCCAGGTCATAG2150.14889402900317178No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCATAGCTGTTTCCTGC2150.14889402900317178No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA2140.1482014986357152No Hit
TGTAAAANGACGGCCAGTGCTAGACATCGCCTGGTGCCTTTGGTCATAGC2030.14058366459369243No Hit
TGTAAAANGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGGG2020.13989113422623584No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGGG2010.1391986038587792No Hit
TGTAAAANGACGGCCAGTTGGTGTGGGCAGTGGTCATAGCTGTTTCCTGC2000.1385060734913226No Hit
TGTAAAACGACGGCCAGTTGGAGGTGGGGCCTGATGGGTCATAGCTGTTT1970.13642848238895275No Hit
TGTAAAANGACGGCCAGTGGTACGTGGTGGTGGCTGAGAGCCCCCTGCCG1970.13642848238895275No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTTAGTATTTGAA1940.13435089128658292No Hit
TGTAAAANGACGGCCAGTTGGAGGTGGGGCCTGATGGGTCATAGCTGTTT1860.12881064834693No Hit
TGTAAAANGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT1840.12742558761201678No Hit
TGTAAAANGACGGCCAGTTCCTTCTTGGGTGGGACGTTGCTACCGGTCAT1820.12604052687710357No Hit
TGTAAAANGACGGCCAGTGGGCAGGGGAGTCAGCAGAGGCCTCGCTCGGG1730.11980775356999404No Hit
TGTAAAACGACGGCCAGTGGGCAGGGGAGTCAGCAGAGGCCTCGCTCGGG1700.11773016246762422No Hit
TGTAAAACGACGGCCAGTTCCTTCTTGGGTGGGACGTTGCTACCGGTCAT1690.11703763210016759No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCTTTGGTCATAGC1650.11426751063034116No Hit
TGTAAAANGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC1640.11357498026288453No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCACCGAGGCAG1630.11288244989542792No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGAGCCCCCTGCCG1620.11218991952797129No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT1600.11080485879305807No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC1560.10803473732323163No Hit
TGTAAAANGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG1540.1066496765883184No Hit
TGTAAAACGACGGCCAGTTGGATGGTGAGGGAAGGGCTAAGACAGGATTC1460.10110943364866548No Hit
TGTAAAACGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT1450.10041690328120888No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)