FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144398
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNGCAGCAAGAGACCCAGACCCACCCGACTGGCC25911.7943461820800841No Hit
CAGGAAANAGCTATGANNGCTTCTATATGAGCGCATCTCACCTGAATTTG25591.7721852103214726No Hit
CAGGAAANAGCTATGANNACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG15731.0893502680092522No Hit
CAGGAAANAGCTATGACCGCAGCAAGAGACCCAGACCCACCCGACTGGCC15521.0748071302926634No Hit
CAGGAAANAGCTATGACCGCTTCTATATGAGCGCATCTCACCTGAATTTG13700.9487666034155597No Hit
CAGGAAANAGCTATGANNACTCCCAACTCTTGGCTGTGCCACTGGCCGTC13290.9203728583498386No Hit
CAGGAAANAGCTATGANNAACCAACACTGCTGCTAATTGTGTTTGTGTCC12810.8871314007119211No Hit
CAGGAAACAGCTATGACCGCAGCAAGAGACCCAGACCCACCCGACTGGCC10690.7403149628111193No Hit
CAGGAAANAGCTATGANCGCAGCAAGAGACCCAGACCCACCCGACTGGCC9880.6842200030471336No Hit
CAGGAAANAGCTATGANNGCAAAACACCTTAACACTCGGCTTCTATTTGC9660.6689843349630882No Hit
CAGGAAANAGCTATGANNTGCCAGTCTTCCCCTTCCCTCACCATCCAACT9530.6599814401861521No Hit
CAGGAAANAGCTATGACCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG9410.6516710757766728No Hit
CAGGAAANAGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA9240.6398980595299104No Hit
CAGGAAANAGCTATGANNTCCTCTTCATTTTCACGGTGCCATTCTGATTC9220.6385129987949971No Hit
CAGGAAANAGCTATGANNGCTTCTATATGAGCGCATCTCACCAACCTTGG9180.6357428773251708No Hit
CAGGAAACAGCTATGACCGCTTCTATATGAGCGCATCTCACCTGAATTTG8980.6218922699760384No Hit
CAGGAAANAGCTATGACCACTCCCAACTCTTGGCTGTGCCACTGGCCGTC8950.6198146788736686No Hit
CAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC8600.5955761160126871No Hit
CAGGAAANAGCTATGANNGCTTCTATATGAGCCCATCTCACCAACCTTGG8230.5699524924167925No Hit
CAGGAAANAGCTATGANCGCTTCTATATGAGCGCATCTCACCTGAATTTG8220.5692599620493358No Hit
CAGGAAANAGCTATGANNTAGAGGACACCCAAGGCCACCAGGGCAGCGGT8160.5651047798445962No Hit
CAGGAAANAGCTATGANNTCAAGCAAGTTCAGGATGGTATAGAAATAGAG7250.5020845164060445No Hit
CAGGAAANAGCTATGACCAACCAACACTGCTGCTAATTGTGTTTGTGTCC7190.49792933420130475No Hit
CAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC6950.481308605382346No Hit
CAGGAAANAGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT6790.4702281195030402No Hit
CAGGAAANAGCTATGACCTGCCAGTCTTCCCCTTCCCTCACCATCCAACT6520.4515297995817117No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG6340.43906425296749263No Hit
CAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA6230.4314464189254699No Hit
CAGGAAANAGCTATGANNTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC6200.42936882782310004No Hit
CAGGAAANAGCTATGANNGCAGCAAGAGACCAATACCCACCCGACTGGCC5990.41482569010651116No Hit
CAGGAAANAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC5900.4085929167994017No Hit
CAGGAAANAGCTATGANCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG5710.39543483981772604No Hit
CAGGAAANAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT5640.3905871272455297No Hit
CAGGAAACAGCTATGACCACTCCCAACTCTTGGCTGTGCCACTGGCCGTC5530.382969293203507No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA5470.37881411099876733No Hit
CAGGAAANAGCTATGANCACTCCCAACTCTTGGCTGTGCCACTGGCCGTC5370.37188880732420115No Hit
CAGGAAANAGCTATGACCGCTTCTATATGAGCGCATCTCACCAACCTTGG5350.3705037465892879No Hit
CAGGAAANAGCTATGACCTCCTCTTCATTTTCACGGTGCCATTCTGATTC5220.361500851812352No Hit
CAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA5040.3490353051981329No Hit
CAGGAAANAGCTATGANNACAGGCAGGAGGGAGTACTACTGGCCGTCGTT5040.3490353051981329No Hit
CAGGAAANAGCTATGANNGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT5030.3483427748306763No Hit
CAGGAAANAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA4980.34488012299339327No Hit
CAGGAAANAGCTATGANNCTCAGCCCTGCAGCAGTTACCACACTGGCCGT4920.3407249407886536No Hit
CAGGAAANAGCTATGACCGCTTCTATATGAGCCCATCTCACCAACCTTGG4880.33795481931882715No Hit
CAGGAAANAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC4820.3337996371140875No Hit
CAGGAAANAGCTATGACCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG4580.31717890829512874No Hit
CAGGAAANAGCTATGACCTAGAGGACACCCAAGGCCACCAGGGCAGCGGT4530.3137162564578457No Hit
CAGGAAANAGCTATGANNTGGAGTTTGAAACAGCACCTTGGAACTGGCCG4480.3102536046205626No Hit
CAGGAAACAGCTATGACCAACCAACACTGCTGCTAATTGTGTTTGTGTCC4470.30956107425310603No Hit
CAGGAAANAGCTATGANCAACCAACACTGCTGCTAATTGTGTTTGTGTCC4350.3012507098436266No Hit
CAGGAAANAGCTATGANNCTAGGGTGGGGTGAGGGGAGTGTACTGGCCGT4350.3012507098436266No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT4350.3012507098436266No Hit
CAGGAAANAGCTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT4320.2991731187412568No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTCACCATCCAACT4080.2825523899222981No Hit
CAGGAAANAGCTATGANNGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC3970.2749345558802753No Hit
CAGGAAANAGCTATGANCGCAAAACACCTTAACACTCGGCTTCTATTTGC3960.27424202551281873No Hit
CAGGAAANAGCTATGANCGCTTCTATATGAGCGCATCTCACCAACCTTGG3900.2700868433080791No Hit
CAGGAAANAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA3880.26870178257316585No Hit
CAGGAAANAGCTATGANCTGCCAGTCTTCCCCTTCCCTCACCATCCAACT3880.26870178257316585No Hit
CAGGAAANAGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT3770.2610839485311431No Hit
CAGGAAANAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC3770.2610839485311431No Hit
CAGGAAANAGCTATGANNAGAGACCAGATGCATAAGCACAGCAGCATTCC3640.25208105375420714No Hit
CAGGAAANAGCTATGANCTCCTCTTCATTTTCACGGTGCCATTCTGATTC3610.25000346265183726No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC3500.24238562860981455No Hit
CAGGAAANAGCTATGACCACAGGCAGGAGGGAGTACTACTGGCCGTCGTT3480.2410005678749013No Hit
CAGGAAANAGCTATGACCGCAGCAAGAGACCAATACCCACCCGACTGGCC3420.23684538567016164No Hit
CAGGAAACAGCTATGACCTCCTCTTCATTTTCACGGTGCCATTCTGATTC3310.2292275516281389No Hit
CAGGAAANAGCTATGACCTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC3210.22230224795357278No Hit
CAGGAAANAGCTATGACCCTCAGCCCTGCAGCAGTTACCACACTGGCCGT3150.21814706574883308No Hit
CAGGAAANAGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT3150.21814706574883308No Hit
CAGGAAANAGCTATGANCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA3150.21814706574883308No Hit
CAGGAAANAGCTATGACCCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA3090.2139918835440934No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCAGGGCAGCGGT3080.2132993531766368No Hit
CAGGAAACAGCTATGACCGCTTCTATATGAGCGCATCTCACCAACCTTGG3060.21191429244172355No Hit
CAGGAAANAGCTATGANNAAGGACAAGGTGAGGCAGCAAGTGCTGATGTA3060.21191429244172355No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA3020.2091441709718971No Hit
CAGGAAANAGCTATGANCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC3010.2084516406044405No Hit
CAGGAAANAGCTATGANCGCTTCTATATGAGCCCATCTCACCAACCTTGG2980.2063740495020707No Hit
CAGGAAANAGCTATGANCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2970.20568151913461402No Hit
CAGGAAACAGCTATGACCGCTTCTATATGAGCCCATCTCACCAACCTTGG2940.20360392803224422No Hit
CAGGAAANAGCTATGANNTAGAGGACACCCAAGGCCACATTGCGACTGGC2910.20152633692987437No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC2880.19944874582750452No Hit
CAGGAAANAGCTATGANCTAGAGGACACCCAAGGCCACCAGGGCAGCGGT2860.1980636850925913No Hit
CAGGAAANAGCTATGANCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG2850.1973711547251347No Hit
CAGGAAANAGCTATGANNGGTAGCAACGTCCCACCCAGGAAGGAACTGGC2800.19390850288785164No Hit
CAGGAAANAGCTATGANCGCAGCAAGAGACCAATACCCACCCGACTGGCC2710.18767572958074213No Hit
CAGGAAANAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA2680.18559813847837228No Hit
CAGGAAANAGCTATGANNGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG2660.18421307774345905No Hit
CAGGAAANAGCTATGACCCTAGGGTGGGGTGAGGGGAGTGTACTGGCCGT2630.18213548664108922No Hit
CAGGAAANAGCTATGANNCGTTAGCTGCCTCTCCTGCCCACTGGCCGTCG2630.18213548664108922No Hit
CAGGAAANAGCTATGANCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA2610.180750425906176No Hit
CAGGAAANAGCTATGANNTGCCAAAATTAAGGATGCACACCTGTGACACA2600.18005789553871937No Hit
CAGGAAANAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT2560.17728777406889293No Hit
CAGGAAACAGCTATGACCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG2540.1759027133339797No Hit
CAGGAAANAGCTATGACCGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT2520.17451765259906646No Hit
CAGGAAANAGCTATGANNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC2510.17382512223160987No Hit
CAGGAAANAGCTATGANCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC2470.1710550007617834No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA2460.1703624703943268No Hit
CAGGAAANAGCTATGACCTGGAGTTTGAAACAGCACCTTGGAACTGGCCG2450.16966994002687016No Hit
CAGGAAACAGCTATGACCACAGGCAGGAGGGAGTACTACTGGCCGTCGTT2450.16966994002687016No Hit
CAGGAAANAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT2430.16828487929195696No Hit
CAGGAAANAGCTATGANNCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC2420.16759234892450034No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC2390.16551475782213051No Hit
CAGGAAACAGCTATGANCGCAGCAAGAGACCCAGACCCACCCGACTGGCC2350.16274463635230405No Hit
CAGGAAANAGCTATGANCTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC2310.1599745148824776No Hit
CAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT2310.1599745148824776No Hit
CAGGAAANAGCTATGANNGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG2300.15928198451502099No Hit
CAGGAAANAGCTATGANNGCTCCAGCAGAGAGCAGCTGGAGGGACTGGCC2270.15720439341265113No Hit
CAGGAAANAGCTATGACCAGAGACCAGATGCATAAGCACAGCAGCATTCC2160.1495865593706284No Hit
CAGGAAACAGCTATGACCGCAGCAAGAGACCAATACCCACCCGACTGGCC2150.14889402900317178No Hit
CAGGAAACAGCTATGANCGCTTCTATATGAGCGCATCTCACCTGAATTTG2070.1433537860635189No Hit
CAGGAAANAGCTATGACCGCTCCAGCAGAGAGCAGCTGGAGGGACTGGCC2050.14196872532860566No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA2040.14127619496114904No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC2030.14058366459369243No Hit
CAGGAAANAGCTATGANCACAGGCAGGAGGGAGTACTACTGGCCGTCGTT2030.14058366459369243No Hit
CAGGAAANAGCTATGANNGTCAACAGCATACCTGTACACCTGAAATAACT2030.14058366459369243No Hit
CAGGAAANAGCTATGANNCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT2020.13989113422623584No Hit
CAGGAAANAGCTATGANCCTCAGCCCTGCAGCAGTTACCACACTGGCCGT1960.13573595202149613No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA1920.1329658305516697No Hit
CAGGAAANAGCTATGANCCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA1910.13227330018421307No Hit
CAGGAAACAGCTATGACCCTCAGCCCTGCAGCAGTTACCACACTGGCCGT1900.13158076981675645No Hit
CAGGAAANAGCTATGANNGGGCAGGCCGAGGACATTAGAAATTTGGGACG1900.13158076981675645No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGTGTACTGGCCGT1870.12950317871438663No Hit
CAGGAAANAGCTATGANCAGGCACCAACAGTACAAAGCAGATACAAAGCA1870.12950317871438663No Hit
CAGGAAACAGCTATGACCGCTCCAGCAGAGAGCAGCTGGAGGGACTGGCC1870.12950317871438663No Hit
CAGGAAANAGCTATGACCCGTTAGCTGCCTCTCCTGCCCACTGGCCGTCG1860.12881064834693No Hit
CAGGAAANAGCTATGANCGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT1790.12396293577473373No Hit
CAGGAAANAGCTATGANNTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG1780.1232704054072771No Hit
CAGGAAANAGCTATGACCGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC1750.12119281430490728No Hit
CAGGAAANAGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT1710.11842269283508082No Hit
CAGGAAANAGCTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT1700.11773016246762422No Hit
CAGGAAANAGCTATGACCTAGAGGACACCCAAGGCCACATTGCGACTGGC1680.11634510173271098No Hit
CAGGAAANAGCTATGACCGGTAGCAACGTCCCACCCAGGAAGGAACTGGC1670.11565257136525436No Hit
CAGGAAANAGCTATGACCAAGGACAAGGTGAGGCAGCAAGTGCTGATGTA1640.11357498026288453No Hit
CAGGAAANAGCTATGANNCTCAGCTCTGCAGCAGTTACCACACTGGCCGT1640.11357498026288453No Hit
CAGGAAANAGCTATGANNTGGAGTTTGAAACAGAACCTTGGAACTGGCCG1640.11357498026288453No Hit
CAGGAAANAGNTATGANNGCAGCAAGAGACCCAGACCCACCCGACTGGCC1610.11149738916051469No Hit
CAGGAAACAGCTATGANCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG1610.11149738916051469No Hit
CAGGAAANAGCTATGANCTGGAGTTTGAAACAGCACCTTGGAACTGGCCG1610.11149738916051469No Hit
CAGGAAANAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT1600.11080485879305807No Hit
CAGGAAANAGCTATGANNTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC1600.11080485879305807No Hit
CAGGAAANAGCTATGANCCTAGGGTGGGGTGAGGGGAGTGTACTGGCCGT1600.11080485879305807No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCCACTGGCCGTCG1600.11080485879305807No Hit
CAGGAAANAGCTATGANNCCTGGACGCAATCACCCAGGCCAGCTAAGTGA1590.11011232842560147No Hit
CAGGAAACAGCTATGACCGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT1560.10803473732323163No Hit
CAGGAAANAGCTATGANCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT1560.10803473732323163No Hit
CAGGAAANAGCTATGANNTCCAGCTGCTCCATCTTGTCTACCTCCTGGAA1500.10387955511849195No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGAACTGGCCG1490.10318702475103535No Hit
CAGGAAANAGCTATGACCGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG1480.10249449438357872No Hit
CAGGAAANAGCTATGANNACTGCCCACACCAACTGGCCGTCGTTTTACAC1470.10180196401612211No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT1460.10110943364866548No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)