FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141028
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC17591.2472700456646908No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC15601.106163315086366No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG13280.9416569759196756No Hit
CAGGAAANAGCTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT13090.9281844740051621No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT12990.9210936835238391No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT12950.9182573673313101No Hit
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC11660.8267861701222453No Hit
CAGGAAANAGCTATGANNCGTAAAGGGCAAGGTGGGGTAATCTTGTCCAT11540.8182772215446579No Hit
CAGGAAANAGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT10810.7665144510310009No Hit
CAGGAAANAGCTATGANNACCCCACCTGCTCAAAAGACACACTCCTAAAA10490.7438239214907678No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA10240.7260969452874607No Hit
CAGGAAANAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC9850.6984428624103015No Hit
CAGGAAANAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC9330.6615707519074226No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG9230.6544799614260998No Hit
CAGGAAANAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG8250.5849902147091358No Hit
CAGGAAANAGCTATGANNTCCCACCACTGACTCCAGGGGAAGGAGGAGGA7920.5615906061207704No Hit
CAGGAAANAGCTATGANNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG7690.5452817880137277No Hit
CAGGAAANAGCTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG7460.5289729699066852No Hit
CAGGAAANAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAACT7420.5261366537141561No Hit
CAGGAAANAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAATT7030.49848257083699693No Hit
CAGGAAANAGCTATGACCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT6860.486428227018748No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT6650.4715375670079701No Hit
CAGGAAANAGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT6540.4637376974785149No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC6350.45026519556400146No Hit
CAGGAAANAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT6300.4467198003233401No Hit
CAGGAAANAGCTATGACCCGTAAAGGGCAAGGTGGGGTAATCTTGTCCAT6270.44459256317894325No Hit
CAGGAAANAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC6270.44459256317894325No Hit
CAGGAAANAGCTATGANCACAGTGTCCCCATCAGGCTCCGGGACAGCATC6250.4431744050826786No Hit
CAGGAAANAGCTATGANNGCAAAACACCTTAACACTCGGCTTCTATTTGC6200.4396290098420172No Hit
CAGGAAANAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA6000.4254474288793715No Hit
CAGGAAANAGCTATGANCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC5840.4141021641092549No Hit
CAGGAAANAGCTATGANNAGGACTTGCCCACTCTCCCAGTTACTTGGAAT5810.41197492696485805No Hit
CAGGAAANAGCTATGANNGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT5790.4105567688685935No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC5510.3907025555208895No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG5260.3729755793175823No Hit
CAGGAAANAGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT5250.37226650026945No Hit
CAGGAAANAGCTATGANNGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT5240.3715574212213178No Hit
CAGGAAANAGCTATGANNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT5160.36588478883625947No Hit
CAGGAAANAGCTATGACCACCCCACCTGCTCAAAAGACACACTCCTAAAA5130.3637575516918626No Hit
CAGGAAANAGCTATGANCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG5110.362339393595598No Hit
CAGGAAANAGCTATGANNAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC5060.35879399835493664No Hit
CAGGAAANAGCTATGANNAAGTTGGTCAGCCCTGGTGACTCATTGGATAT5000.3545395240661429No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG4870.3453214964404232No Hit
CAGGAAANAGCTATGANCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT4780.3389397850072326No Hit
CAGGAAANAGCTATGACCTCCCACCACTGACTCCAGGGGAAGGAGGAGGA4770.3382307059591003No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAACT4730.33539438976657115No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAATT4680.33184899452590977No Hit
CAGGAAANAGCTATGANCGCATCAGATTCACTTCCACTGTCGTCAGAATT4590.32546728309271916No Hit
CAGGAAANAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG4580.3247582040445869No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT4560.32334004594832233No Hit
CAGGAAANAGCTATGANCGCATCAGATTCACTTCCACTGTCGTCAGAACT4310.3056130697450152No Hit
CAGGAAANAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG4290.3041949116487506No Hit
CAGGAAANAGCTATGANCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC4270.302776753552486No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT4210.2985222792636923No Hit
CAGGAAANAGCTATGANNCTGGGTGCCGGGTAGAGTGTATACAGGGCTCA4150.2942678049748986No Hit
CAGGAAACAGCTATGACCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT4120.29214056783050174No Hit
CAGGAAACAGCTATGACCCGTAAAGGGCAAGGTGGGGTAATCTTGTCCAT4110.29143148878236946No Hit
CAGGAAANAGCTATGACCGTCCTAGCACCGCAGCGAATGGGACCCGCAAG3990.282922540204782No Hit
CAGGAAANAGCTATGANNTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC3930.2786680659159883No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC3920.27795898686785603No Hit
CAGGAAANAGCTATGANNGCCCCTACTCCAAAAGGCGGATGAATGGGGAA3880.2751226706753269No Hit
CAGGAAANAGCTATGANNGCTGCTGACCCAACCCTACCCTGTCCAGCCCC3870.2744135916271946No Hit
CAGGAAANAGCTATGACCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT3820.2708681963865332No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA3780.26803188019400404No Hit
CAGGAAANAGCTATGANCCGTAAAGGGCAAGGTGGGGTAATCTTGTCCAT3740.26519556400147487No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT3730.2644864849533426No Hit
CAGGAAANAGCTATGANNGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG3690.2616501687608135No Hit
CAGGAAANAGCTATGANCAGGCACCAACAGTACAAAGCAGATACAAAGCA3670.2602320106645489No Hit
CAGGAAANAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT3670.2602320106645489No Hit
CAGGAAANAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC3660.2595229316164166No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTATGTGCA3580.2538502992313583No Hit
CAGGAAANAGCTATGANNGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA3550.25172306208696144No Hit
CAGGAAANAGCTATGANNTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC3510.24888674589443233No Hit
CAGGAAANAGCTATGANCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT3500.24817766684630005No Hit
CAGGAAANAGCTATGANNGCCCTGGCCACTGTGGTTAATGAAACTTGGTT3460.24534135065377086No Hit
CAGGAAANAGCTATGANNAAACTATTAATACCTGCAGCAAAGCCCCAAGC3420.24250503446124175No Hit
CAGGAAANAGCTATGANCACCCCACCTGCTCAAAAGACACACTCCTAAAA3200.22690529540233145No Hit
CAGGAAANAGCTATGACCGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT3140.22265082111353773No Hit
CAGGAAANAGCTATGANNCCTGGACGCAATCACCCAGGCCAGCTAAGTGA3140.22265082111353773No Hit
CAGGAAANAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG3100.2198145049210086No Hit
CAGGAAACAGCTATGACCACCCCACCTGCTCAAAAGACACACTCCTAAAA3090.21910542587287632No Hit
CAGGAAANAGCTATGACCAGGACTTGCCCACTCTCCCAGTTACTTGGAAT3090.21910542587287632No Hit
CAGGAAANAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT3000.21272371443968574No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT2970.21059647729528888No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2960.2098873982471566No Hit
CAGGAAANAGCTATGANCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2860.20279660776583372No Hit
CAGGAAANAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT2790.19783305442890772No Hit
CAGGAAANAGCTATGANNGGAGAAGCACAGCTTCGGCTCTGGCCCTGGGG2770.1964148963326432No Hit
CAGGAAANAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT2730.19357858014011403No Hit
CAGGAAANAGCTATGANNCCCTGCGGCTCCTTTCAAGTTAAGTCACCACT2670.1893241058513203No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT2630.18648778965879115No Hit
CAGGAAANAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT2620.1857787106106589No Hit
CAGGAAANAGCTATGANCTCCCACCACTGACTCCAGGGGAAGGAGGAGGA2600.1843605525143943No Hit
CAGGAAACAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG2590.18365147346626204No Hit
CAGGAAANAGCTATGANCACACCCGGAGCAGTGCAAATCAACTCCCCCAG2570.18223331536999743No Hit
CAGGAAANAGCTATGANNCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT2550.18081515727373287No Hit
CAGGAAANAGCTATGANNCAGAAGGCTGAGCAGGAGGCTGGGAAAAAGGG2540.18010607822560057No Hit
CAGGAAANAGCTATGANCGTCCTAGCACCGCAGCGAATGGGACCCGCAAG2540.18010607822560057No Hit
CAGGAAANAGCTATGANNGGTAGCAACGTCCCACCCAGCTGAATAGTTGT2520.17868792012933601No Hit
CAGGAAANAGCTATGANNCTTGGTGGAGCCATAGGCCGCATGCACGCGCA2510.17797884108120374No Hit
CAGGAAANAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT2500.17726976203307146No Hit
CAGGAAANAGCTATGACCAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC2490.17656068298493915No Hit
CAGGAAACAGCTATGACCTCCCACCACTGACTCCAGGGGAAGGAGGAGGA2490.17656068298493915No Hit
CAGGAAANAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT2400.1701789715517486No Hit
CAGGAAANAGCTATGANNGGTCCCGCGGGGAAAGTAAGTCCTTCCCTTGG2370.16805173440735174No Hit
CAGGAAACAGCTATGACCGTCCTAGCACCGCAGCGAATGGGACCCGCAAG2360.16734265535921944No Hit
CAGGAAANAGCTATGACCAAACTATTAATACCTGCAGCAAAGCCCCAAGC2350.16663357631108716No Hit
CAGGAAANAGCTATGANNTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC2340.16592449726295488No Hit
CAGGAAANAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC2310.16379726011855802No Hit
CAGGAAANAGCTATGACCCTGGGTGCCGGGTAGAGTGTATACAGGGCTCA2260.16025186487789658No Hit
CAGGAAANAGCTATGANCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT2250.1595427858297643No Hit
CAGGAAANAGCTATGACCGCCCCTACTCCAAAAGGCGGATGAATGGGGAA2240.15883370678163203No Hit
CAGGAAANAGCTATGANNGGGCAGGCCGAGGACATTAGAAATTTGGGACG2240.15883370678163203No Hit
CAGGAAANAGCTATGANNACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG2230.15812462773349972No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT2170.153870153444706No Hit
CAGGAAANAGCTATGANCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT2120.1503247582040446No Hit
CAGGAAANAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC2090.14819752105964773No Hit
CAGGAAANAGCTATGANCGCAAAACACCTTAACACTCGGCTTCTATTTGC2060.14607028391525087No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC2050.1453612048671186No Hit
CAGGAAANAGCTATGANCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG2050.1453612048671186No Hit
CAGGAAANAGCTATGANCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT2040.14465212581898632No Hit
CAGGAAANAGCTATGANCAGGACTTGCCCACTCTCCCAGTTACTTGGAAT2030.143943046770854No Hit
CAGGAAANAGCTATGANNTCCACAAGGCCCTGGGACAATCTCCCAGGCCC2030.143943046770854No Hit
CAGGAAANAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC2000.14181580962645715No Hit
CAGGAAANAGCTATGANCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT2000.14181580962645715No Hit
CAGGAAANAGCTATGANCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT2000.14181580962645715No Hit
CAGGAAANAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA1980.1403976515301926No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT1970.1396885724820603No Hit
CAGGAAANAGCTATGACCGCCCTGGCCACTGTGGTTAATGAAACTTGGTT1930.13685225628953115No Hit
CAGGAAANAGCTATGANNGCTACGGGCAATCACTCTCCTACTCCCAACCA1920.13614317724139888No Hit
CAGGAAANAGCTATGANCGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT1890.13401594009700202No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1860.13188870295260516No Hit
CAGGAAACAGCTATGACCAGGACTTGCCCACTCTCCCAGTTACTTGGAAT1860.13188870295260516No Hit
CAGGAAANAGCTATGANCAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC1840.13047054485634058No Hit
CAGGAAANAGCTATGACCGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG1840.13047054485634058No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT1800.12763422866381144No Hit
CAGGAAANAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA1720.12196159627875316No Hit
CAGGAAACAGCTATGACCGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT1670.11841620103809174No Hit
CAGGAAACAGCTATGACCCTGGGTGCCGGGTAGAGTGTATACAGGGCTCA1650.11699804294182715No Hit
CAGGAAANAGCTATGANNCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA1630.11557988484556259No Hit
CAGGAAANAGCTATGANNGCTACGGGCAATCACTCTCCTATTCCCAACCA1630.11557988484556259No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT1610.11416172674929802No Hit
CAGGAAANAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT1600.11345264770116573No Hit
CAGGAAANAGCTATGANNACTCCTCCTGGCCTTCCTCTCCAGTCGGTACT1600.11345264770116573No Hit
CAGGAAACAGCTATGACCAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC1600.11345264770116573No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT1590.11274356865303344No Hit
CAGGAAANAGCTATGANNTCCAGTAAAACTGCCTCTGTTTCAACTCCCTT1530.10848909436423973No Hit
CAGGAAANAGCTATGACCGGGCAGGCCGAGGACATTAGAAATTTGGGACG1520.10778001531610744No Hit
CAGGAAACAGCTATGANCACAGTGTCCCCATCAGGCTCCGGGACAGCATC1520.10778001531610744No Hit
CAGGAAANAGCTATGANCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT1520.10778001531610744No Hit
CAGGAAACAGCTATGACCGCCCCTACTCCAAAAGGCGGATGAATGGGGAA1500.10636185721984287No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC1490.10565277817171058No Hit
CAGGAAANAGCTATGANCCTGGGTGCCGGGTAGAGTGTATACAGGGCTCA1480.1049436991235783No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT1470.10423462007544601No Hit
CAGGAAANAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT1450.10281646197918144No Hit
CAGGAAANAGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1450.10281646197918144No Hit
CAGGAAANAGCTATGACCGGAGAAGCACAGCTTCGGCTCTGGCCCTGGGG1440.10210738293104915No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC1430.10139830388291686No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)