FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131353
Sequences flagged as poor quality0
Sequence length300
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC33782.5716961165713763No Hit
CAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA23101.758619902095879No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT20741.5789513753016682No Hit
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC20111.5309890143354168No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC15611.1884007217193364No Hit
CAGGAAANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC14171.0787724680821908No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG13481.0262422632143917No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG11660.8876843315341104No Hit
CAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT10780.8206892876447436No Hit
CAGGAAANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA10420.7932822242354571No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT10060.7658751608261707No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT9470.720958029127618No Hit
CAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT9310.708777112056824No Hit
CAGGAAANAGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT8670.660053443773648No Hit
CAGGAAANAGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT8590.6539629852382511No Hit
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC8270.6296011510966631No Hit
CAGGAAANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC7800.5938197072012059No Hit
CAGGAAANAGCTATGANNAGAGACCAGATGCATAAGCACAGCAGCATTCC7240.551186497453427No Hit
CAGGAAANAGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT7220.5496638828195778No Hit
CAGGAAANAGCTATGANNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG6900.5253020486779898No Hit
CAGGAAANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC6450.4910432194163818No Hit
CAGGAAANAGCTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT6280.47810099502866327No Hit
CAGGAAANAGCTATGANNCCGTAGCCACGCCCCTATTGTCTGACCACGCT6020.458307004788623No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCTGAGGGATGGG5900.44917131698552754No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG5690.43318386333011044No Hit
CAGGAAANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG5470.41643510235776876No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA5230.39816372675157785No Hit
CAGGAAANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG5020.38217627309616076No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG4900.3730405852930652No Hit
CAGGAAANAGCTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA4850.3692340487084422No Hit
CAGGAAANAGCTATGANNTAGAGGACACCCAAGGCCACCAGGGCAGCGGT4720.35933705358842205No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA4660.3547692096868743No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC4490.3418269852991557No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG4400.33497521944683406No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT4280.32583953164373863No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC4270.32507822432681405No Hit
CAGGAAANNGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT4220.3212716877421909No Hit
CAGGAAANAGCTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT4180.31822645847449244No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT4130.3144199218898693No Hit
CAGGAAANAGCTATGANNGGAGGCTGAGGTGGGAGGATCGCTTGATCCCG4100.3121359999390954No Hit
CAGGAAANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT3870.2946259316498291No Hit
CAGGANANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC3820.29081939506520593No Hit
NAGGAAANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC3750.2854902438467336No Hit
CAGGAAANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT3720.28320632189595973No Hit
CAGGAAANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT3590.27330932677593966No Hit
NAGGANANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC3570.2717867121420904No Hit
CAGGAAANAGCTATGANNACTCCCAACTCCCGTCTGGTCGGGGCAAAAAC3450.2626510243389949No Hit
CAGGAAANAGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT3350.2550379511697487No Hit
CAGGAAANNGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT3260.2481861853174271No Hit
CAGGAAANAGCTATGANNAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG3190.2428570340989547No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3090.2352439609297085No Hit
CAGGAAANAGCTATGANNAGTGGGAGGAGCTCTCGGTCCCCTCTTTTAGG3030.23067611702816074No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG3000.2283921950773869No Hit
CAGGAAANAGCTATGANNGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG2980.22686958044353767No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG2960.2253469658096884No Hit
CAGGAAANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2850.21697258532351757No Hit
CAGGAAANAGCTATGANNGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC2840.21621127800659293No Hit
NAGGANANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA2780.21164343410504516No Hit
CAGGAAANNGCTATGANNAGAGACCAGATGCATAAGCACAGCAGCATTCC2770.21088212678812057No Hit
NAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2730.2078368975204221No Hit
CAGGAAANAGCTATGANNTGGGCCTGGACACTTTGACGGCGGGGCACGTC2730.2078368975204221No Hit
CAGGANANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA2660.2025077463019497No Hit
NAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2570.1956559804496281No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA2450.1865202926465326No Hit
CAGGAAANNGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT2450.1865202926465326No Hit
CAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2410.18347506337883412No Hit
NAGGAAANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA2370.18042983411113564No Hit
CAGGAAANAGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT2350.17890721947728638No Hit
CAGGAAANNGCTATGANNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG2300.17510068289266328No Hit
NAGGANANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2270.1728167609418894No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA2260.1720554536249648No Hit
CAGGAAANNGCTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT2240.17053283899111554No Hit
CAGGAAANAGCTATGANNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG2200.16748760972341703No Hit
CAGGAAANNGCTATGANNACGTTCTGGGCTGTGGTGTGCTGAGGGATGGG2190.16672630240649244No Hit
CAGGAAANNGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA2180.1659649950895678No Hit
CAGGAAACNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC2170.1652036877726432No Hit
CAGGAAANNGCTATGANNCCGTAGCCACGCCCCTATTGTCTGACCACGCT2150.16368107313879393No Hit
CAGGAAANNGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG2090.15911322923724622No Hit
CAGGAANNNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC2070.15759061460339696No Hit
CAGGANANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2040.1553066926526231No Hit
NAGGAAANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2030.15454538533569848No Hit
CAGGAAACAGCTATGANCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1920.1461710048495276No Hit
CAGGAAACAGCNATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1890.14388708289875374No Hit
CAGGAAANNGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1830.139319238997206No Hit
CAGGAAACNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1780.1355127024125829No Hit
NAGGAAANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1760.13399008777873364No Hit
CAGGANANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1760.13399008777873364No Hit
NAGGAAANAGCTATGANCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1760.13399008777873364No Hit
CAGGAAANAGCTATGANNGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG1750.13322878046180903No Hit
NAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1740.1324674731448844No Hit
CAGGAAANNGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC1740.1324674731448844No Hit
CAGGAAANAGCTATGANNTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG1720.13094485851103513No Hit
NAGGANANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1710.13018355119411051No Hit
CAGGAAANAGCTATGANNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG1680.12789962924333664No Hit
CAGGAAANNGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG1680.12789962924333664No Hit
CAGGAAANNGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC1650.1256157072925628No Hit
CAGGAAANNGCTATGANNGGAGGCTGAGGTGGGAGGATCGCTTGATCCCG1640.12485439997563816No Hit
CAGGAAANNGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT1640.12485439997563816No Hit
CAGGAANNNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1610.1225704780248643No Hit
CAGGAAANNGCTATGANNTAGAGGACACCCAAGGCCACCAGGGCAGCGGT1610.1225704780248643No Hit
CAGGAAANNGCTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA1590.12104786339101506No Hit
CAGGAAACNGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1580.12028655607409042No Hit
CAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1570.11952524875716582No Hit
CAGGAAACAGCTATGANCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1490.11343479022176883No Hit
NAGGAAANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1470.11191217558791956No Hit
NAGGAAANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1460.11115086827099495No Hit
NAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1450.11038956095407032No Hit
CAGGAAANAGCTATGANNCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC1450.11038956095407032No Hit
CAGGAAANAGCTATGANNAAGTTGGTCAGCCCTGGTGACTCATTGGATAT1430.10886694632022108No Hit
CAGGANANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1420.10810563900329646No Hit
CAGGAAANAGCTATGANNGCCCTGGCCACTGTGGTTAATGAAACTTGGTT1420.10810563900329646No Hit
CAGGAAANAGCTATGANNAGACAGGGTCTTGCTATGTTGCCCAGGCTGGT1400.10658302436944722No Hit
CAGGAAACAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1400.10658302436944722No Hit
CAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG1380.10506040973559798No Hit
CAGGAANNNGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1370.10429910241867334No Hit
CAGGAAACAGCNATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1360.10353779510174872No Hit
CAGGAAANNGCTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT1350.1027764877848241No Hit
NAGGANANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1340.10201518046789948No Hit
CAGGAAANAGCTATGANNCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA1330.10125387315097485No Hit
NAGGANANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1330.10125387315097485No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)