FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160047
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG20661.2908708067005317No Hit
CAGGAAANAGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC14070.879116759451911No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA13350.8341299743200435No Hit
CAGGAAANAGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT12520.7822702081263628No Hit
CAGGAAANAGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC12370.7728979612238905No Hit
CAGGAAANAGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT12150.7591519991002643No Hit
CAGGAAANAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG11530.7204133785700451No Hit
CAGGAAANAGCTATGANNACAAACGCAAATGGCTTTTAGCATTTCACAGG9770.6104456815810355No Hit
CAGGAAANAGCTATGANNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC9720.6073215992802115No Hit
CAGGAAANAGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA9400.587327472554937No Hit
CAGGAAANAGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA8720.5448399532637288No Hit
CAGGAAANAGCTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC8660.5410910545027398No Hit
CAGGAAANAGCTATGACCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC8220.5135991302554874No Hit
CAGGAAANAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA7840.489856104769224No Hit
CAGGAAANAGCTATGANNATCACACCACAGCACTCCAGCACTGGCCGTCG7630.4767349591057627No Hit
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG7580.4736108768049386No Hit
CAGGAAANAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT7530.4704867945041144No Hit
CAGGAAANAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT7490.4679875286634551No Hit
CAGGAAANAGCTATGANNATCGCACCACTGCACTCCAGCACTGGCCGTCG6890.4304985410535655No Hit
CAGGAAANAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC6890.4304985410535655No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC6880.42987372459340073No Hit
CAGGAAANAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG6860.4286240916730711No Hit
CAGGAAANAGCTATGANNCAGGGTGACTCCGATCCTGGAGGACAAGGCGG6080.37988840778021454No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG5970.3730154267184015No Hit
CAGGAAANAGCTATGANNTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG5810.36301836335576426No Hit
CAGGAAANAGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG5680.3548957493736215No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTATGTGCA5600.34989721769230286No Hit
CAGGAAANAGCTATGANNTGAAGTGGCTCCATCCTTCCATTTGTTTATAT5470.34177460371016016No Hit
CAGGAAANAGCTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT5430.3392753378695008No Hit
CAGGAAANAGCTATGANNCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA5360.33490162264834705No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT5250.32802864158653394No Hit
CAGGAAANAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG5190.324279742825545No Hit
CAGGAAANAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA5190.324279742825545No Hit
CAGGAAANAGCTATGACCGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC5170.3230301099052153No Hit
CAGGAAANAGCTATGACCATCACACCACAGCACTCCAGCACTGGCCGTCG5080.3174067617637319No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG5070.31678194530356707No Hit
CAGGAAACAGCTATGACCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC5060.31615712884340225No Hit
CAGGAAANAGCTATGANNTCAAGCAAGTTCAGGATGGTATAGAAATAGAG5050.3155323123832374No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA5040.3149074959230726No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT4790.29928708441895197No Hit
CAGGAAANAGCTATGANCAGGCACCAACAGTACAAAGCAGATACAAAGCA4710.2942885527376333No Hit
CAGGAAANAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA4710.2942885527376333No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT4670.291789286896974No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT4670.291789286896974No Hit
CAGGAAANAGCTATGANCTGGGGATGAGGACAGCAGGGCAGTCACCATCT4650.29053965397664433No Hit
CAGGAAANAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC4640.2899148375164795No Hit
CAGGAAANAGCTATGANCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC4520.28241703999450163No Hit
CAGGAAANAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC4460.27866814123351263No Hit
CAGGAAANAGCTATGANNCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG4430.2767936918530182No Hit
CAGGAAANAGCTATGACCATCGCACCACTGCACTCCAGCACTGGCCGTCG4420.27616887539285334No Hit
CAGGAAANAGCTATGANCGGGACCAGTCCAATAACCCCTGAGGGGTATAT4410.2755440589326885No Hit
CAGGAAANAGCTATGANNTGACAGCACAAGGAACACTGACCTACAGATAA4320.26992071079120505No Hit
CAGGAAANAGCTATGANNCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC4310.26929589433104023No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC4090.25554993220741407No Hit
CAGGAAANAGCTATGANNTCCAGTAAAACTGCCTCTGTTTCAACTCCCTT3900.24367841946428237No Hit
CAGGAAACAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG3740.23368135610164512No Hit
CAGGAAACAGCTATGACCATCACACCACAGCACTCCAGCACTGGCCGTCG3740.23368135610164512No Hit
CAGGAAANAGCTATGANNTGCCCAGTTGTTTCCAGAACCTTGTTTTTGAC3740.23368135610164512No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAGCTGGAGGGAC3720.2324317231813155No Hit
CAGGAAANAGCTATGACCTGAAGTGGCTCCATCCTTCCATTTGTTTATAT3620.22618355857966724No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG3600.2249339256593376No Hit
CAGGAAANAGCTATGANCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC3540.22118502689834862No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT3530.2205602104381838No Hit
CAGGAAANAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG3520.21993539397801898No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC3470.2168113116771948No Hit
CAGGAAANAGCTATGANNCGCTTCTGGGGACTTTGCTCTTGGGCTCTTTT3440.21493686229670037No Hit
CAGGAAANAGCTATGANNAAGTTGGTCAGCCCTGGTGACTCATTGGATAT3430.21431204583653554No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA3410.21306241291620584No Hit
CAGGAAANAGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT3410.21306241291620584No Hit
CAGGAAANAGCTATGANCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA3330.20806388123488723No Hit
CAGGAAANAGCTATGANNGTCAACAGCATACCTGTACACCTGAAATAACT3290.20556461539422793No Hit
CAGGAAACAGCTATGACCGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC3240.2024405330934038No Hit
CAGGAAANAGCTATGANCGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC3230.20181571663323897No Hit
CAGGAAANAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA3210.20056608371290932No Hit
CAGGAAANAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG3210.20056608371290932No Hit
CAGGAAACAGCTATGACCATCGCACCACTGCACTCCAGCACTGGCCGTCG3170.19806681787225006No Hit
CAGGAAANAGCTATGACCCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA3160.19744200141208518No Hit
CAGGAAANAGCTATGANCACAAACGCAAATGGCTTTTAGCATTTCACAGG3150.19681718495192035No Hit
CAGGAAANAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT3120.19494273557142588No Hit
CAGGAAANAGCTATGANNGCTGCTGACCCAACCCTACCCTGTCCAGCCCC3080.1924434697307666No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA3080.1924434697307666No Hit
CAGGAAANAGCTATGANCATCACACCACAGCACTCCAGCACTGGCCGTCG3010.1880697545096128No Hit
CAGGAAANAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG2970.18557048866895348No Hit
CAGGAAANAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG2970.18557048866895348No Hit
CAGGAAANAGCTATGANNGGCAGACAGCCTCTCCTCCTTTCATCACAAAA2930.1830712228282942No Hit
CAGGAAANAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG2920.18244640636812937No Hit
CAGGAAANAGCTATGANCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC2870.17932232406730522No Hit
CAGGAAANAGCTATGANCATCGCACCACTGCACTCCAGCACTGGCCGTCG2860.1786975076071404No Hit
CAGGAAANAGCTATGANCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA2810.17557342530631628No Hit
CAGGAAANAGCTATGACCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG2730.17057489362499767No Hit
CAGGAAANAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT2730.17057489362499767No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC2720.16995007716483285No Hit
CAGGAAACAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC2670.1668259948640087No Hit
CAGGAAANAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT2660.16620117840384388No Hit
CAGGAAANAGCTATGANNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT2610.16307709610301974No Hit
CAGGAAANAGCTATGANNTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC2590.16182746318269006No Hit
CAGGAAANAGCTATGANCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC2540.15870338088186595No Hit
CAGGAAANAGCTATGACCCATGTGCCCCAGTAGAGAGCAGCTGGAGGGAC2540.15870338088186595No Hit
CAGGAAACAGCTATGACCTGAAGTGGCTCCATCCTTCCATTTGTTTATAT2510.15682893150137148No Hit
CAGGAAANAGCTATGANNGCCCTGGCCACTGTGGTTAATGAAACTTGGTT2410.1505807668997232No Hit
CAGGAAANAGCTATGACCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG2400.1499559504395584No Hit
CAGGAAANAGCTATGANNCCTGGACGCAATCACCCAGGCCAGCTAAGTGA2390.14933113397939357No Hit
CAGGAAANAGCTATGACCTGACAGCACAAGGAACACTGACCTACAGATAA2380.14870631751922872No Hit
CAGGAAACAGCTATGACCCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA2370.1480815010590639No Hit
CAGGAAANAGCTATGACCTCCAGTAAAACTGCCTCTGTTTCAACTCCCTT2320.14495741875823975No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC2250.14058370353708596No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT2240.13995888707692114No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA2230.13933407061675632No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2200.13745962123626185No Hit
CAGGAAANAGCTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG2190.13683480477609702No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC2180.1362099883159322No Hit
CAGGAAANAGCTATGANCTGAAGTGGCTCCATCCTTCCATTTGTTTATAT2120.13246108955494323No Hit
CAGGAAACAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT2110.1318362730947784No Hit
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2090.13058664017444876No Hit
CAGGAAANAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT2040.12746255787362462No Hit
CAGGAAANAGCTATGANCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2010.12558810849313015No Hit
CAGGAAANAGCTATGACCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC2000.1249632920329653No Hit
CAGGAAANAGCTATGANCTTCATACAGAGATACAAAGGCAACTATGTGCA1990.12433847557280049No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC1980.12371365911263567No Hit
CAGGAAANAGCTATGACCTGCCCAGTTGTTTCCAGAACCTTGTTTTTGAC1960.122464026192306No Hit
CAGGAAANAGCTATGANNAATGCAAGGGACCACCAGGCACGGTGGCTCAT1950.12183920973214118No Hit
CAGGAAANAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG1930.12058957681181154No Hit
CAGGAAANAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT1920.11996476035164672No Hit
CAGGAAANAGCTATGANCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG1920.11996476035164672No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG1910.11933994389148186No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG1910.11933994389148186No Hit
CAGGAAANAGCTATGANCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG1910.11933994389148186No Hit
CAGGAAANAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC1890.11809031097115223No Hit
CAGGAAANAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGGCTCTTTT1890.11809031097115223No Hit
CAGGAAANAGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1880.11746549451098741No Hit
CAGGAAACAGCTATGACCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG1880.11746549451098741No Hit
CAGGAAANAGCTATGANCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG1870.11684067805082256No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG1850.11559104513049293No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT1840.1149662286703281No Hit
CAGGAAANAGCTATGANCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT1840.1149662286703281No Hit
CAGGAAANAGCTATGACCGTCAACAGCATACCTGTACACCTGAAATAACT1830.11434141221016325No Hit
CAGGAAANAGCTATGANCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG1820.11371659574999843No Hit
CAGGAAANAGCTATGANCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT1810.11309177928983362No Hit
CAGGAAANAGCTATGANCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG1800.1124669628296688No Hit
CAGGAAACAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG1800.1124669628296688No Hit
CAGGAAACAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG1800.1124669628296688No Hit
CAGGAAANAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT1790.11184214636950394No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGCAGAGAGCAGCTGGAGGGAC1790.11184214636950394No Hit
CAGGAAANAGCTATGANNTCCCACTTGCTCTTTGAAGGCAATTCCATTCT1780.11121732990933912No Hit
CAGGAAANAGCTATGACCCATGTGCCCCAGCAGAGAGCAGCTGGAGGGAC1770.11059251344917431No Hit
CAGGAAANAGCTATGACCGGCAGACAGCCTCTCCTCCTTTCATCACAAAA1770.11059251344917431No Hit
CAGGAAANAGCTATGANNACCTGGCAGCCCCAAATCTGCACCTCCCGCGG1760.10996769698900949No Hit
CAGGAAANAGCTATGANCCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA1720.10746843114835018No Hit
CAGGAAANAGCTATGANNTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG1710.10684361468818536No Hit
CAGGAAANAGCTATGANNACCCCTTAAGCCAGAGCCGACTGGCCGTCGTT1690.10559398176785569No Hit
CAGGAAANAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC1660.1037195323873612No Hit
CAGGAAANAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1640.10246989946703156No Hit
CAGGAAACAGCTATGACCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC1630.10184508300686675No Hit
CAGGAAANAGCTATGANCCATGTGCCCCAGTAGAGAGCAGCTGGAGGGAC1610.10059545008653707No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAGCTGGAGGGAC1610.10059545008653707No Hit
CAGGAAANAGCTATGANCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT1610.10059545008653707No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)