FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107140
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAANGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC25262.3576628710098935No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC23822.2232592869143177No Hit
TGTAAAANGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG14711.3729699458652231No Hit
TGTAAAANGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG14691.3711032294194512No Hit
TGTAAAANGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC14341.3384356916184432No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG13551.2647003920104536No Hit
TGTAAAANGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC13401.2507000186671644No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG13091.2217659137577002No Hit
TGTAAAANGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC13051.2180324808661565No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC12691.1844315848422624No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC12571.1732312861676313No Hit
TGTAAAANGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT12211.139630390143737No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC12061.125630016800448No Hit
TGTAAAANGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG11771.0985626283367558No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT10610.9902930744819861No Hit
TGTAAAANGACGGCCAGTTGAGAGGGACTGGAGAAAGGGAACTTACAGAG10350.9660257606869517No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG10350.9660257606869517No Hit
TGTAAAACGACGGCCAGTTGAGAGGGACTGGAGAAAGGGAACTTACAGAG10290.960425611349636No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA10130.9454918797834608No Hit
TGTAAAANGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA10060.9389583722232593No Hit
TGTAAAANGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA10040.9370916557774873No Hit
TGTAAAANGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA9380.8754900130670151No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA9170.8558894903864103No Hit
TGTAAAANGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC8860.826955385476946No Hit
TGTAAAANGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC8440.7877543401157365No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC8380.7821541907784206No Hit
TGTAAAANGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC7960.7429531454172111No Hit
TGTAAAANGACGGCCAGTGAGACAGGCTTCTGGGCTGTCCACAACTCTCT7920.7392197125256673No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA7920.7392197125256673No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC7750.7233526227366063No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC7520.7018853836102296No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC7390.6897517267127123No Hit
TGTAAAACGACGGCCAGTGAGACAGGCTTCTGGGCTGTCCACAACTCTCT7200.6720179204778794No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC7110.6636176964719059No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG6880.6421504573455292No Hit
TGTAAAANGACGGCCAGTCTCTCACCCACCTCCACCCATCAAAGGTGCCT6790.6337502333395557No Hit
TGTAAAANGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT6670.6225499346649244No Hit
TGTAAAANGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC6250.5833488893037148No Hit
TGTAAAANGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG6180.5768153817435131No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT6080.5674817995146537No Hit
TGTAAAANGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC5950.5553481426171365No Hit
TGTAAAACGACGGCCAGTCTCTCACCCACCTCCACCCATCAAAGGTGCCT5890.5497479932798208No Hit
TGTAAAANGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC5760.5376143363823035No Hit
TGTAAAANGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG5710.5329475452678738No Hit
TGTAAAANGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG5640.5264140377076721No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG5540.5170804554788128No Hit
TGTAAAANGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG5470.5105469479186111No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC5360.5002800074668657No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC5250.49001306701512043No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC5000.4666791114429718No Hit
TGTAAAANGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCAGTGGG5000.4666791114429718No Hit
TGTAAAANGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG4910.45827888743699835No Hit
TGTAAAANGACGGCCAGTTGCCTTTGTATCTCTGTATGAAGGTCATAGCT4760.4442785140937091No Hit
TGTAAAANGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCCGTGGG4620.431211498973306No Hit
TGTAAAANGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGTCTTGGTGGT4580.4274780660817622No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG4540.42374463319021843No Hit
TGTAAAANGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG4510.4209445585215606No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG4480.41814448385290276No Hit
TGTAAAACGACGGCCAGTTGCCTTTGTATCTCTGTATGAAGGTCATAGCT4410.4116109762927011No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG4200.39201045361209635No Hit
TGTAAAANGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC4190.3910770953892103No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC4180.39014373716632444No Hit
TGTAAAANGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG4110.3836102296061228No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGTCTTGGTGGT4090.38174351316035093No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCCGTGGG4020.37521000560014933No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCAGTGGG3980.37147657270860557No Hit
TGTAAAANGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA3830.35747619936531644No Hit
TGTAAAANGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC3830.35747619936531644No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC3740.34907597535934287No Hit
TGTAAAANGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG3720.347209258913571No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG3690.3444091842449132No Hit
TGTAAAANGACGGCCAGTAGCAGGCAGGTCCTTCCAGACAGCCAATGAGG3690.3444091842449132No Hit
TGTAAAANGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT3570.3332088855702819No Hit
TGTAAAANGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC3560.33227552734739596No Hit
TGTAAAANGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGGG3540.33040881090162405No Hit
TGTAAAANGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA3450.32200858689565054No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG3380.31547507933544894No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGGG3370.314541721112563No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA3290.3070748553294754No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC3280.3061414971065895No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT3280.3061414971065895No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG3240.30240806421504574No Hit
TGTAAAANGACGGCCAGTATCTCCTGGGGAGAAACGGCATCTGTGTCCAA3080.2874743326488706No Hit
TGTAAAACGACGGCCAGTTCCCACCACCTGCCTTCCGGTCATAGCTGTTT3070.2865409744259847No Hit
TGTAAAANGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA3060.28560761620309877No Hit
TGTAAAANGACGGCCAGTCCATTACTGCTGCGTCCCTGTTTTACAATGCA3040.28374089975732686No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA2990.27907410864289717No Hit
TGTAAAANGACGGCCAGTCCTCACTCCCCACCCAGGATCTGGGCACTGGA2990.27907410864289717No Hit
TGTAAAACGACGGCCAGTATCTCCTGGGGAGAAACGGCATCTGTGTCCAA2970.27720739219712526No Hit
TGTAAAANGACGGCCAGTATCTCCTGGGGAGAAACGGCTTCTGTGTCCAA2850.2660070935224939No Hit
TGTAAAACGACGGCCAGTAGCAGGCAGGTCCTTCCAGACAGCCAATGAGG2840.265073735299608No Hit
TGTAAAACGACGGCCAGTATCTCCTGGGGAGAAACGGCTTCTGTGTCCAA2830.26414037707672205No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCTGGCCCGGTCAT2780.2594735859622923No Hit
TGTAAAANGACGGCCAGTTCCCACCACCTGCCTTCCGGTCATAGCTGTTT2720.25387343662497663No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTTTTACAATGCA2680.25014000373343287No Hit
TGTAAAANGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG2660.24827328728766102No Hit
TGTAAAANGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA2630.24547321261900318No Hit
TGTAAAACGACGGCCAGTCCTCACTCCCCACCCAGGATCTGGGCACTGGA2620.24453985439611722No Hit
TGTAAAANGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG2590.2417397797274594No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA2530.23613963039014374No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT2510.23427291394437183No Hit
TGTAAAANGACGGCCAGTTGTGTGTTCCTGCTCCTGGCTGGCCCGGTCAT2510.23427291394437183No Hit
TGTAAAACGACGGCCAGTAGAACAATGCGGCACTGAAGGAAGAGATGATT2450.22867276460705618No Hit
TGTAAAANGACGGCCAGTAGAACAATGCGGCACTGAAGGAAGAGATGATT2430.2268060481612843No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC2430.2268060481612843No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC2360.2202725406010827No Hit
TGTAAAACGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG2350.21933918237819675No Hit
TGTAAAANGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT2350.21933918237819675No Hit
TGTAAAANGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC2320.2165391077095389No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG2210.20627216725779357No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA2170.20253873436624978No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC2150.20067201792047787No Hit
TGTAAAANGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT2130.198805301474706No Hit
TGTAAAANGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT1890.17640470412544335No Hit
TGTAAAANGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTA1880.1754713459025574No Hit
TGTAAAANGACGGCCAGTAAACCTGGCCCCACTGCCCTGCCTCTCCCAGG1810.1689378383423558No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT1810.1689378383423558No Hit
TGTAAAANGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA1730.16147097255926826No Hit
TGTAAAACGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA1690.15773753966772447No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT1660.15493746499906663No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTA1630.15213739033040882No Hit
TGTAAAANGACGGCCAGTGGATCCAGGTAGGTGTCCAGGTCACTGCCAAT1570.14653724099309315No Hit
TGTAAAANGACGGCCAGTAAACCTGGCCCCACTGCCCTGCCTGTCCCAGG1510.14093709165577747No Hit
TGTAAAANGACGGCCAGTTGATGGACTGGATGCTGCCGTCTTGGCTCACT1500.14000373343289155No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA1500.14000373343289155No Hit
TGTAAAANGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA1460.13627030054134776No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCCTGCCTCTCCCAGG1440.13440358409557587No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCTTGGCTCACT1440.13440358409557587No Hit
TGTAAAANGACGGCCAGTTGATGGACTGGATGCTGCCGTATGGGCTCACT1420.132536867649804No Hit
TGTAAAACGACGGCCAGTGGATCCAGGTAGGTGTCCAGGTCACTGCCAAT1380.12880343475826023No Hit
TGTAAAANGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT1370.1278700765353743No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCCTGCCTGTCCCAGG1350.1260033600896024No Hit
TGTAAAANGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG1260.11760313608362889No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAGCC1220.11386970319208513No Hit
TGTAAAANGACGGCCAGTTGGAGTGTCTAGGTTTGAAAGCTGCTGGTCAT1220.11386970319208513No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTATGGGCTCACT1210.11293634496919917No Hit
TGTAAAACGACGGCCAGTTGGAGTGTCTAGGTTTGAAAGCTGCTGGTCAT1190.11106962852342728No Hit
TGTAAAACGACGGCCAGTGAACAGCCCCGGAAGCAAGTAAAAGGAGGAGG1170.10920291207765541No Hit
TGTAAAANGACGGCCAGTGAACAGCCCCGGAAGCAAGTAAAAGGAGGAGG1150.10733619563188353No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)