FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173701
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG50532.9090218248599604No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG47882.756460814848504No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC38482.2153010057512623No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA38372.2089682845809753No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT37352.150246688274679No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG37302.1473681786518215No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC36042.074829736155808No Hit
TGTAAAACGACGGCCAGTCCAGAGATCAATAATAGCTCAGGGGAGATGGA33531.9303285530883532No Hit
TGTAAAACGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT32911.894635033764918No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA32321.8606686202151974No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG31531.815188168174046No Hit
TGTAAAACGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG31151.793311495040328No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA30791.7725862257557525No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT30511.7564665718677497No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC28761.6557187350677314No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA25601.4737969269031266No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT22121.2734526571522329No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG20941.2055198300527918No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA17831.026476531511045No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCCAAGAGGTGGGG16790.9666035313556053No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT16190.9320614158813132No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT16090.9263043966355979No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC15150.8721884157258738No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG14550.8376463002515818No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC14520.8359191944778672No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG14350.8261322617601511No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA14260.8209509444390072No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT13780.7933172520595737No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACCTGGCGCAGGC13540.7795004058698568No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC13320.7668349635292832No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC13240.7622293481327108No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC13180.7587751365852816No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC12850.739776973074421No Hit
TGTAAAACGACGGCCAGTAGGTCATCCTCCTGCCTCGGCCTCCCAAAGTG12320.7092647710721297No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGCACATGGTT12210.7029320499018429No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC11690.672995549824123No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCAAGAGGTGGGGT11470.6603301074835494No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA11380.6551487901624055No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG11160.6424833478218318No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG10210.5877916649875361No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA10120.5826103476663923No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCATCAGGTTCCC10060.579156136118963No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG9800.5641878860801032No Hit
TGTAAAACGACGGCCAGTTGAAGAATGGTGGCTTGTTTCATGGTGGCCTT9470.5451897225692426No Hit
TGTAAAACGACGGCCAGTTCCTGGGCTTCTCTTGAGTACGGAGGAAAGTG9160.527342962907525No Hit
TGTAAAACGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGGGACACGGA8720.5020120782263775No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG8530.4910737416595184No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTGCGGCCCCTGA8380.48243821279094534No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG8320.47898400124351614No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCACCGAGGCAG8130.46804566467665704No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGTCATAGCTGTT7670.4415633761463664No Hit
TGTAAAACGACGGCCAGTATCTCCTGGGGAGAAACGGCTTCTGTGTCCAA7540.4340792511269365No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG6990.4024156452755022No Hit
TGTAAAACGACGGCCAGTTCTTCACCTCCCAAAATGCTGGGATTACAGGC6980.40183994335093065No Hit
TGTAAAACGACGGCCAGTCTCTCACCCACCTCCACCCATCAAAGGTGCCT6810.39205301063321457No Hit
TGTAAAACGACGGCCAGTAGCAGGCAGGTCCTTCCAGACAGCCAATGAGG6660.3834174817646416No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC5650.3252715873829166No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG5030.2895780680594815No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCTCCTGCTCAGCCTT4990.2872752603611954No Hit
TGTAAAACGACGGCCAGTTGGAGTGCCTGGGTTAGAACCCTGCTGGTACC4800.2763369237943362No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCTGGAGTGCAGTG4710.2711556064731924No Hit
TGTAAAACGACGGCCAGTATCTCCTGGGGAGAAACGGCATCTGTGTCCAA4660.26827709685033474No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTTTTACAATGCA4630.26654999107662014No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA4510.25964156798176175No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGGTCATAGCTGTT4430.25503595258518946No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCTGCTGTCCTCA4430.25503595258518946No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGCTCTGGTG4380.25215744296233183No Hit
TGTAAAACGACGGCCAGTCTGGGTTGGGGTGTCTGCTGTTTCCTGTTGTG4320.24870323141490264No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGGAGGGTCATAGC4180.24064340447090116No Hit
TGTAAAACGACGGCCAGTGCCATCGGCTCCCAAGTTTTATGTCCTAGAGC4170.24006770254632964No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGTCATAGCTGTTT3900.22452375058289822No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGAGGGTCATAGCT3880.22337234673375514No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG3860.22222094288461205No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTTGCGGCCCCTG3720.21416111594061057No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCCAGGTCATAGC3650.21013120246860983No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCACATGCCTCCC3630.2089797986194668No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCATGTGAAGAGAG3550.2043741832228945No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCCCTGCACATGCAAG3450.19861716397717916No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT3440.19804146205260764No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG3370.1940115485806069No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGGGTCATAGCTGT3350.19286014473146382No Hit
TGTAAAACGACGGCCAGTACCTGAATCTTACTAAGCGCCTGGATCTATTG3340.1922844428068923No Hit
TGTAAAACGACGGCCAGTCCTCACTCCCCACCCAGGATCTGGGCACTGGA3160.1819218081646047No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC3070.17674049084346088No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCAGGTCATAGCTG2890.1663778562011733No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGCATTTCCTTGGC2830.16292364465374407No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCAATCATCTAGG2720.1565909234834572No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGGTGCGAGT2670.15371241386059953No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGGGGCCCGGATGT2620.15083390423774187No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGGCACTCTTGGTG2590.14910679846402727No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGGATCATGCTGC2560.14737969269031265No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACGTTGCTACCGG2430.13989556767088274No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTAGGATTTTTTTTT2400.1381684618971681No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCAGTGGTGCGATG2370.13644135612345354No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGCACATGGGT2370.13644135612345354No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTTAGTATTTGAA2280.13126003880230971No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGGGAGGGAGGAG2150.12377591378287978No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAAAGCCACAGCCCTCAG2080.11974600031087904No Hit
TGTAAAACGACGGCCAGTTGCCCAACGTTGTAGTGTTGAGTGCATGGCAT2060.11859459646173597No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGATATGACAACAAT1940.11168617336687757No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGCGCCTG1760.10132353872458995No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)