FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188325
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG55572.950750033187309No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA50742.69427850789858No Hit
TGTAAAACGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTCTGGCATGG50442.678348599495553No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGCTACGGGGT46042.4447099429178283No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCTGTAAAACGACG45912.43780698260985No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCAAGGGT44212.347537501659365No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC44002.336386565777247No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA42912.278507898579583No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA41312.193548387096774No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCAGTGGTGCGATG37291.9800876144962165No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC36261.9253949289791583No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC34811.8484003716978628No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG29591.5712199654851984No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG28871.5329881853179346No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC25031.329085357759193No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC21831.159166334793575No Hit
TGTAAAACGACGGCCAGTCCAGAGATCAATAATAGCTCAGGGGAGATGGA20881.108721624850657No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC19551.0380990309305722No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC18420.9780963759458383No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC17940.9526085225009957No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC16430.8724279835390947No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGGCTACGGGGTCA16070.8533120934554627No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT15230.8087083499269879No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC15080.8007433957254746No Hit
TGTAAAACGACGGCCAGTAGTGACAACAGCCCCAAAGGAATGATCCCGCT14230.7556086552502324No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCTGTGCTCGGGAA13880.7370237621133678No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT13500.7168458781362007No Hit
TGTAAAACGACGGCCAGTGGGGTGGGGAGTGAGATGGTCCTAGGAAGTAC13040.6924200185848931No Hit
TGTAAAACGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG11120.5904686048055224No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC10500.5575467941059339No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGTAAAACGACG9150.4858622062923138No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCAGTGGTGCGATGGTCATAGCTG8520.4524093986459578No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC7920.4205495818399044No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT7890.4189565909996018No Hit
TGTAAAACGACGGCCAGTCGCCTGAATAGGTGCAGGGGCACGAGCTCGGC7390.3924067436612239No Hit
TGTAAAACGACGGCCAGTAACAAATTCAGGTGAGATGCGCTCATATAGAA7080.3759458383114297No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTGGTGGTCC6860.36426390548254345No Hit
TGTAAAACGACGGCCAGTGGAGCGACTTGACAATGCCCCTCACACCCCTT6760.3589539360148679No Hit
TGTAAAACGACGGCCAGTCACCAGCCATCTGGACCTGGTCATAGCTGTTT6360.33771405814416566No Hit
TGTAAAACGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG6360.33771405814416566No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC6360.33771405814416566No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCATTTTGGGCCTGG5980.31753617416699853No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA5680.30160626576397187No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG5470.2904553298818532No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGTGACC5240.2782424001061994No Hit
TGTAAAACGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA5030.2670914642240807No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTGTAAAACGACGG4980.2644364794902429No Hit
TGTAAAACGACGGCCAGTCAGTTCCCAGGGACTCTGCTCTATGAGAGAGA4510.2394796229921678No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC4450.23629364131156244No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAGCC4220.22408071153590864No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC3910.20761980618611442No Hit
TGTAAAACGACGGCCAGTCGCCTGAATAGGTGCAGGGGCACGATATCCAT3880.20602681534581177No Hit
TGTAAAACGACGGCCAGTACACTCCCTCCACCTCCCATCCCAGAAAAAGT3540.18797291915571485No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGAGCCTTTGCAC3260.17310500464622328No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGAAACC3250.17257400769945574No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGTGCCTG2720.14443116952077525No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGTAACC2450.13009425195805124No Hit
TGTAAAACGACGGCCAGTCGCCTGAATAGGTGCAGGGGCGTGATCAAGGC2410.12797026417098104No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCAATGGTGCAATC2390.1269082702774459No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTTCCTGTCCAGGC2300.1221292977565379No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCATAATCTGCTTTT2290.12159830080977034No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG2230.11841231912916501No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGACCAGACG2190.11628833134209478No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG2180.11575733439532722No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC2170.11522633744855967No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCTGAGTA2110.11204035576795432No Hit
TGTAAAACGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTTTCCTGTCA2110.11204035576795432No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCCT2100.11150935882118679No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCAATAGGGGCGTGGCTACGGGGT2080.11044736492765166No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCATCAACATCATCC2030.10779238019381389No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCAAGTA2000.10619938935351123No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTGCAACCTCAG1980.1051373954599761No Hit
TGTAAAACGACGGCCAGTTGCTTCGGGGCATAGACAGCGGAGGGGGGTCA1980.1051373954599761No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTA1940.10301340767290589No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)