FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188325
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC33801.7947696800743393No Hit
CAGGAAANAGCTATGANNCCGTAGCCACGCCCCTATTGCAGGAAACAGCT32321.7161821319527413No Hit
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC30761.6333466082570025No Hit
CAGGAAANAGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT27621.46661356697199No Hit
CAGGAAANAGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT25311.3439532722686844No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG21921.163945307314483No Hit
CAGGAAANAGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC21521.1427054294437806No Hit
CAGGAAANAGCTATGANNAGGTCCAGATGGCTGGTGACTGGCCGTCGTTT20611.0943847072879331No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT19461.0333200584096642No Hit
CAGGAAANAGCTATGANNCTTGAGCTCCTCGGACACGGCAGGAAACAGCT17890.9499535377671577No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC17310.9191557148546395No Hit
CAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT16110.8554360812425329No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG16060.8527810965086952No Hit
CAGGAAANAGCTATGANNATCGCACCACTGCACTCCAGCAGGAAACAGCT13770.7311827956989247No Hit
CAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC12140.6446302933758131No Hit
CAGGAAANNGCTATGANNCCGTAGCCACGCCCCTATTGCAGGAAACAGCT12100.6425063055887428No Hit
CAGGAAANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC11330.601619540687641No Hit
CAGGAAANAGCTATGANNCCGTAGCCACACCCCTTAAGCCAGAGCCGACT10400.5522368246382583No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG10250.544271870436745No Hit
CAGGAAANNGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT9940.5278109650869507No Hit
CAGGAAANNGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT9890.525155980353113No Hit
CAGGAAANAGCTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC9660.5129430505774591No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA9340.4959511482808974No Hit
CAGGAAANAGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG8710.4624983406345413No Hit
CAGGAAANNGCTATGANNAGGTCCAGATGGCTGGTGACTGGCCGTCGTTT8700.4619673436877738No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA8570.4550643833797956No Hit
CAGGAAANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG8420.44709942917828227No Hit
CAGGAAANAGCTATGANNCTTGAGCTCCTCAGCCACCACCACGTACCACT7650.4062126642771804No Hit
CAGGAAANNGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC7260.3855037833532457No Hit
CAGGAAANNGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT7100.3770078322049648No Hit
CAGGAAANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC6980.37063586884375416No Hit
CAGGAAANAGCTATGANNATCGCACCACTGCGCTCCATACACCTGGGACA6920.3674498871631488No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGCGTGTCCGCTGAG6790.3605469268551706No Hit
CAGGAAANAGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT6730.35736094517456524No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC6720.3568299482277977No Hit
CAGGAAANNGCTATGANNCTTGAGCTCCTCGGACACGGCAGGAAACAGCT6390.33930704898446834No Hit
CAGGAAANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT6360.33771405814416566No Hit
CAGGAAANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG6210.32974910394265233No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA5970.317005177220231No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA5610.29788928713659896No Hit
CAGGAAANNGCTATGANNATCGCACCACTGCACTCCAGCAGGAAACAGCT5320.28249037568033986No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG4990.26496747643701046No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC4470.2373556352050976No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA4310.22885968405681667No Hit
CAGGAAANAGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA4240.22514270542944378No Hit
CAGGAAANNGCTATGANNCCGTAGCCACACCCCTTAAGCCAGAGCCGACT4170.22142572680207087No Hit
CAGGAAANAGCTATGANNATCGCACCACTGCACTCCAGCACTGGCCGTCG4110.21823974512146554No Hit
CAGGAAANNGCTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC3800.20177883977167133No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC3780.2007168458781362No Hit
CAGGAAANAGCTATGANNGCAAAACACCTTAACACTCGGCTTCTATTTGC3770.20018584893136865No Hit
CAGGAAANNGCTATGANNCGGGACTTCCCGAGCACAGCGTGTCCGCTGAG3650.19381388557015797No Hit
CAGGAAANAGCTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA3630.19275189167662285No Hit
CAGGAAANNGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA3630.19275189167662285No Hit
CAGGAAANNGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG3580.19009690694278508No Hit
CAGGAAANAGCTATGANNCTGGCCTCCTTCAATGCATTTAATGGCCTTAT3570.18956590999601752No Hit
CAGGAAANNGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA3560.18903491304924996No Hit
NAGGANANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3430.18213195274127172No Hit
CAGGAAANNGCTATGANNCTTGAGCTCCTCAGCCACCACCACGTACCACT3420.1816009557945042No Hit
CAGGAAANAGCTATGANNTCCTCTTCATTTTCACGGTGCCATTCTGATTC3400.18053896190096907No Hit
CAGGANANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3320.17629098632682863No Hit
CAGGAAANAGCTATGANNGGAGGCTGAGGTGGGAGGATCGCTTGATCCCG3310.17575998938006107No Hit
NAGGAAANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC3290.17469799548652595No Hit
CAGGANANNGCTATGANNCCGTAGCCACGCCCCTATTGCAGGAAACAGCT3240.17204301075268819No Hit
NAGGAAANNGCTATGANNCCGTAGCCACGCCCCTATTGCAGGAAACAGCT3220.17098101685915307No Hit
CAGGAAANNGCTATGANNATCGCACCACTGCGCTCCATACACCTGGGACA3220.17098101685915307No Hit
NAGGANANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC3210.1704500199123855No Hit
NAGGANANNGCTATGANNCCGTAGCCACGCCCCTATTGCAGGAAACAGCT3210.1704500199123855No Hit
CAGGAAANAGCTATGANNTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC3190.1693880260188504No Hit
NAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3170.16832603212531527No Hit
CAGGAAANAGCTATGANNAATGCAAGGGACCACCAGGCCCAAAATGAGGC3170.16832603212531527No Hit
CAGGAAANAGCTATGANNCCGTAGCCACCCCTTAAGCCAGAGCCGACTGG3160.1677950351785477No Hit
CAGGAAANAGCTATGANNCTGGCCTCCTTCAATGCATTTAATGGCCTTCT3140.16673304128501262No Hit
CAGGAAANNGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG3010.15983008097703438No Hit
CAGGAAANNGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA2940.1561131023496615No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC2920.15505110845612638No Hit
CAGGANANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2900.15398911456259126No Hit
CAGGAAANAGCTATGANNGCAGCTGGGAGATGCCTGGACTGGCCGTCGTT2890.1534581176158237No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG2860.15186512677552103No Hit
NAGGANANNGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT2790.14814814814814814No Hit
CAGGANANNGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT2710.1439001725740077No Hit
CAGGAAANNGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC2650.14071419089340234No Hit
CAGGAAANAGCTATGANNGCTTCTATATGAGCGCATCTCACCTGAATTTG2630.13965219699986725No Hit
CAGGAAANAGCTATGANNCTTGAGCTCCTCGGACACGGACCCTTGAGCTC2620.1391212000530997No Hit
CAGGAAANAGCTATGANNCCGTAGCCACGCCCCTATTGCCAGAGCCGACT2510.1332802336386566No Hit
CAGGAAANAGCTATGANNCCTGGACGCAATCACCCAGGCCAGCTAAGTGA2480.13168724279835392No Hit
NAGGAAANNGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT2470.13115624585158636No Hit
CAGGANANNGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT2420.12850126111774857No Hit
NAGGANANNGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT2410.12797026417098104No Hit
NAGGAAANNGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT2370.1258462763839108No Hit
NAGGANANNGCTATGANNAGGTCCAGATGGCTGGTGACTGGCCGTCGTTT2330.12372228859684056No Hit
CAGGAAANAGCTATGANNTGAAGTGGCTCCATCCTTCCATTTGTTTATAT2300.1221292977565379No Hit
CAGGAAANAGCTATGANNCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG2280.12106730386300278No Hit
CAGGAAACAGCTATGANCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2260.12000530996946768No Hit
CAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA2220.11788132218239744No Hit
NAGGANANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2200.11681932828886234No Hit
NAGGAAANAGCTATGANNCCGTAGCCACGCCCCTATTGCAGGAAACAGCT2180.11575733439532722No Hit
CAGGAANNNGCTATGANNCCGTAGCCACGCCCCTATTGCAGGAAACAGCT2170.11522633744855967No Hit
NAGGAAANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2150.11416434355502456No Hit
CAGGAAANNGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA2150.11416434355502456No Hit
NAGGAAANNGCTATGANNAGGTCCAGATGGCTGGTGACTGGCCGTCGTTT2140.11363334660825701No Hit
NAGGAAANNGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC2100.11150935882118679No Hit
CAGGANANNGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC2090.11097836187441922No Hit
NAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2060.10938537103411655No Hit
CAGGAAANNGCTATGANNATCGCACCACTGCACTCCAGCACTGGCCGTCG2030.10779238019381389No Hit
NAGGANANNGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC2000.10619938935351123No Hit
CAGGAAACAGCNATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1990.10566839240674367No Hit
CAGGAAACAGCTATGANCACAGTGTCCCCATCAGGCTCCGGGACAGCATC1980.1051373954599761No Hit
CAGGAAANNGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1980.1051373954599761No Hit
CAGGANANNGCTATGANNAGGTCCAGATGGCTGGTGACTGGCCGTCGTTT1970.10460639851320856No Hit
CAGGAAANNGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT1950.10354440461967343No Hit
CAGGANANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1940.10301340767290589No Hit
CAGGAAANAGCTATGANNTGCCCAGTTGTTTCCAGAACCTTGTTTTTGAC1910.10142041683260321No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)