FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003245913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219087
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG113485.179677479722667No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC100394.582197939631287No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG71353.25669711119327No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA66643.041714022283385No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG64362.937645775422549No Hit
TGTAAAACGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT47672.1758479508140605No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG45772.0891244117633634No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG43551.9877948029778123No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGTGAACGAAG38061.7372094190892202No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC35511.6208173008896007No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC35011.5979953169288914No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCTGGAGGGCGGCCA34301.5655880997046834No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC33811.543222555423188No Hit
TGTAAAACGACGGCCAGTTGAGAGGGACTGGAGAAAGGGAACTTACAGAG32681.4916448716719841No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC31761.4496524211842783No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC30851.4081164103757866No Hit
TGTAAAACGACGGCCAGTGGGGTGGGGAGTGAGATGGTCCTAGGAAGTAC25541.16574694071305No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA25111.1461200345068396No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTTGGACCCAGAG21790.9945820610077274No Hit
TGTAAAACGACGGCCAGTTGGATGGTGAGGGAAGGGCTAAGACAGGATTC21510.98180174998973No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG20510.9361577820683108No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC19560.8927960125429624No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA16640.7595156262124179No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG15970.7289341677050669No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG15220.6947011917640025No Hit
TGTAAAACGACGGCCAGTAGGAGGGCGGAGGAGGAAAGCAGGTTTGCTCT14920.6810080013875767No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCCTGCCTCTCCCAGG14420.658186017426867No Hit
TGTAAAACGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT14000.6390155508998708No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTTCCTATCG13400.6116291701470192No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA13300.6070647733548773No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA12470.5691802799800992No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT12290.5609643657542437No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC11930.5445325373025328No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC10990.5016272074563985No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC10910.49797569002268505No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG10810.49341129323054306No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA10300.47013286959061923No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGGGGCCCGGATGT8920.4071441938590606No Hit
TGTAAAACGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC8630.3939074431618489No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC8070.3683468211258541No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC7810.3564793894662851No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA7110.32452861192129157No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG6870.3135740596201509No Hit
TGTAAAACGACGGCCAGTTCAGGGAGGCGGGGCTCGTTCCACAGGGACAC6740.3076403437903664No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTATTTCCCTACTT6390.29166495501786965No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG5130.2341535554368812No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAGCC4990.22776339992788253No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT4980.22730696024866834No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT4940.2254812015318116No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGCGCCTG4350.19855126045817414No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGTGCCTG4090.18668382879860512No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCGCCTCGGCCTCC3910.17846791457274963No Hit
TGTAAAACGACGGCCAGTGCCATCGGCTCCCAAGTTTTATGTCCTAGAGC3820.1743599574598219No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC3420.15610237029125418No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTA3380.15427661157439737No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTGCTGTGATCACA3240.14788645606539869No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGGGCACGCCTTC3220.14697357670697028No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGGCCCTGCT3220.14697357670697028No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT3220.14697357670697028No Hit
TGTAAAACGACGGCTAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG3190.14560425766932772No Hit
TGTAAAACGACGGCTAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC3140.14332205927325675No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTTCCC3090.14103986087718579No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCGTCTACACGAGA3030.1383012228019006No Hit
TGTAAAACGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT3010.13738834344347223No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAACCCCGGTCGGGTTCTGG2940.13419326568897286No Hit
TGNAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG2890.1319110672929019No Hit
TGTAAAACGACGGCCAGTGGCTCCCCAGCTGTCTCTCTCCTCTATGGGCT2850.13008530857604514No Hit
TGNAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGCGAGACGGGCACC2660.12141295467097545No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCGCAGCCCTCCTAC2590.1182178769164761No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCAGGCGCCTG2550.11639211819961934No Hit
TGTAAAACGACGGCTAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG2480.11319704044511997No Hit
TGAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGGG2480.11319704044511997No Hit
TGTAAAACGACGGCTAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG2420.11045840236983483No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACCGTGAGACGGGCACC2320.10589400557769288No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)