FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165194
Sequences flagged as poor quality0
Sequence length300
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAANGACGGCCAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA49773.012821288908798No Hit
TGTAAAACGACGGCCAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA43452.6302408077775223No Hit
TGTAAAANGACGGCCAGTCAGCCAGTAGCAACAGCAGCTACTGATGTAAG36652.218603581243871No Hit
TGTAAAACGACGGCCAGTCAGCCAGTAGCAACAGCAGCTACTGATGTAAG34802.106614041672216No Hit
TGTAAAANGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA32641.9758586873615265No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA28751.7403779798297758No Hit
TGTAAAANGACGGCCAGTTCCCAAAGGCTGGTGGAGGAGATGCTGAAGAT27691.67621100039953No Hit
TGTAAAACGACGGCCAGTTCCCAAAGGCTGGTGGAGGAGATGCTGAAGAT24761.4988437836725306No Hit
TGTAAAANGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG24451.4800779689334964No Hit
TGTAAAANGACGGCCAGTCCAGAGATCAATAATAGCTCAGGGGAGATGGA24291.4703923871327045No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG22781.378984708887732No Hit
TGTAAAACGACGGCCAGTCCAGAGATCAATAATAGCTCAGGGGAGATGGA22751.3771686623000834No Hit
TGTAAAANGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTCTGGCATGG22641.370509824812039No Hit
TGTAAAANGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT20001.2106977250989746No Hit
TGTAAAACGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTCTGGCATGG19631.1882998171846435No Hit
TGTAAAANGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT18931.1459253968061796No Hit
TGTAAAANGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA18371.1120258605034081No Hit
TGTAAAANGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC17741.0738888821627905No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA17271.0454374856229645No Hit
TGTAAAACGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT17141.0375679504098212No Hit
TGTAAAACGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT16761.0145646936329407No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC16440.9951935300313571No Hit
TGTAAAANGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT14570.8819932927346029No Hit
TGTAAAANGACGGCCAGTCCCAGCACAACTTCCCCCATGGCGTGGTGTCC14060.8511205007445791No Hit
TGTAAAANGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA13770.8335653837306439No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA13120.7942177076649274No Hit
TGTAAAANGACGGCCAGTTGACACAGCTGCTTCTGAGTACAAGTATGTAG12620.763950264537453No Hit
TGTAAAACGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT12590.7621342179498045No Hit
TGTAAAANGACGGCCAGTTCATAGCTGTGAAGAGGGATACCAAGGAAAGT12590.7621342179498045No Hit
TGTAAAACGACGGCCAGTTCATAGCTGTGAAGAGGGATACCAAGGAAAGT12480.7554753804617601No Hit
TGTAAAANGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC12460.7542646827366611No Hit
TGTAAAACGACGGCCAGTCCCAGCACAACTTCCCCCATGGCGTGGTGTCC12000.7264186350593848No Hit
TGTAAAANGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC11500.6961511919319103No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC11430.691913749894064No Hit
TGTAAAACGACGGCCAGTTGACACAGCTGCTTCTGAGTACAAGTATGTAG11040.6683051442546339No Hit
TGTAAAANGACGGCCAGTAGGTCATCCTCCTGCCTCGGCCTCCCAAAGTG10740.6501446783781493No Hit
TGTAAAANGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG10500.6356163056769617No Hit
TGTAAAANGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT10150.6144290954877295No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT9980.6041381648243883No Hit
TGTAAAANGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC9860.5968739784737944No Hit
TGTAAAANGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT9850.5962686296112449No Hit
TGTAAAACGACGGCCAGTAGGTCATCCTCCTGCCTCGGCCTCCCAAAGTG9560.5787135125973097No Hit
TGTAAAANGACGGCCAGTTGAGAAGCTGAGGCTTAAAGAGGGATCCCTTG9550.5781081637347603No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG9540.5775028148722108No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC9350.5660011864837706No Hit
TGTAAAACGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC9340.5653958376212211No Hit
TGTAAAANGACGGCCAGTTCTTCACCTCCCAAAATGCTGGGATTACAGGC9140.5532888603702314No Hit
TGTAAAANGACGGCCAGTGCCCTGAACCGGTGCCAAATGGAGTGAAGAAA9060.5484460694698354No Hit
TGTAAAANGACGGCCAGTACACATTCTTGTAAAGCATGGGATTGCTGAAT8770.5308909524559003No Hit
TGTAAAACGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT8730.5284695570057024No Hit
TGTAAAACGACGGCCAGTACACATTCTTGTAAAGCATGGGATTGCTGAAT8370.5066769979539208No Hit
TGTAAAACGACGGCCAGTTGAGAAGCTGAGGCTTAAAGAGGGATCCCTTG8070.4885165320774362No Hit
TGTAAAANGACGGCCAGTAAGGGTGGGTCATGGGGTGAGGGTGGGTCGCG8070.4885165320774362No Hit
TGTAAAANGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT7870.4764095548264464No Hit
TGTAAAACGACGGCCAGTTCTTCACCTCCCAAAATGCTGGGATTACAGGC7560.4576437400874124No Hit
TGTAAAACGACGGCCAGTGCCCTGAACCGGTGCCAAATGGAGTGAAGAAA6980.4225335060595421No Hit
TGTAAAANGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCATTTATT6980.4225335060595421No Hit
TGTAAAANGACGGCCAGTGCATGAGGCGAATTCTCAGCCATGACAAGGCT6950.42071745947189365No Hit
TGTAAAACGACGGCCAGTAAGGGTGGGTCATGGGGTGAGGGTGGGTCGCG6950.42071745947189365No Hit
TGTAAAANGACGGCCAGTTCCTGGGCTTCTCTTGAGTACGGAGGAAAGTG6930.4195067617467947No Hit
TGTAAAANGACGGCCAGTTTGCTGCTGGGCTTCCTCTGCCCTGAGGGCTT6810.4122425753962008No Hit
TGTAAAACGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCATTTATT6740.4080051333583544No Hit
TGTAAAANGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA6680.4043730401830575No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT6570.3977142026950132No Hit
TGTAAAANGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG6540.39589815610736473No Hit
TGTAAAACGACGGCCAGTTCCTGGGCTTCTCTTGAGTACGGAGGAAAGTG6530.3952928072448152No Hit
TGTAAAANGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT6430.3892393186193203No Hit
TGTAAAANGACGGCCAGTGCATGAGGCGAATTCTCAGCCGTGACAAGGCT6290.38076443454362746No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCATGACAAGGCT6010.36381466639224186No Hit
TGTAAAACGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG5860.3547344334539996No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCGTGACAAGGCT5830.3529183868663511No Hit
TGTAAAANGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGACCAGACG5780.34989164255360367No Hit
TGTAAAACGACGGCCAGTTTGCTGCTGGGCTTCCTCTGCCCTGAGGGCTT5610.33960071189026236No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA5610.33960071189026236No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT5040.30509582672494157No Hit
TGTAAAANGACGGCCAGTCCACCTGCTTCGCACCATCTCGGCCCAGCCCA5030.30449047786239214No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGACCAGACG4920.29783164037434773No Hit
TGTAAAANGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG4890.2960155937866993No Hit
TGTAAAANGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT4760.2881460585735559No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT4680.28330326767316005No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG4450.26938024383452186No Hit
TGTAAAANGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC4400.2663534995217744No Hit
TGTAAAACGACGGCCAGTCCACCTGCTTCGCACCATCTCGGCCCAGCCCA4370.26453745293412595No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG4210.25485187113333413No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC4030.24395559160744337No Hit
TGTAAAANGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG3960.23971814956959694No Hit
TGTAAAANGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG3840.23245396321900313No Hit
TGTAAAACGACGGCCAGTCCTGCCGCCTGGTCTCACCTCGCCATGGTTCG3740.22640047459350823No Hit
TGTAAAANGACGGCCAGTCCTGCCGCCTGGTCTCACCTCGCCATGGTTCG3630.21974163710546385No Hit
TGTAAAANGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC3580.21671489279271647No Hit
TGTAAAANGACGGCCAGTGGGGACAAGGGTGGCTTTGTAAATAGCAGCAA3140.190079542840539No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG3130.1894741939779895No Hit
TGTAAAANGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG2960.17918326331464823No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG2940.17797256558954927No Hit
TGTAAAANGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG2880.17434047241425232No Hit
TGTAAAANGACGGCCAGTGCCATCGGCTCCCAAGTTTTATGTCCTAGAGC2860.17312977468915336No Hit
TGTAAAANGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT2810.17010303037640592No Hit
TGTAAAANGACGGCCAGTCCTGCCGCCTGGTCTCACCTCGCTATGGTTCG2800.16949768151385644No Hit
TGTAAAACGACGGCCAGTGGGGACAAGGGTGGCTTTGTAAATAGCAGCAA2760.16707628606365849No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC2750.166470937201109No Hit
TGTAAAACGACGGCCAGTGCCATCGGCTCCCAAGTTTTATGTCCTAGAGC2730.16526023947601No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT2690.16283884402581206No Hit
TGTAAAACGACGGCCAGTCCTGCCGCCTGGTCTCACCTCGCTATGGTTCG2440.1477051224620749No Hit
TGTAAAACGACGGCCAGTAGCCAAGATGGTGCCACTGCACTCCAGCCTGG2370.1434676804242285No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG2340.14165163383658003No Hit
TGTAAAANGACGGCCAGTACCTGAATCTTACTAAGCGCCTGGATCTATTG2320.14044093611148106No Hit
TGTAAAANGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA2310.13983558724893155No Hit
TGTAAAANGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC2190.13257140089833772No Hit
TGTAAAANGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG2180.13196605203578823No Hit
TGTAAAANGACGGCCAGTTCTATTGCTGAGACTTTCCAGAGCATTTTGCA2170.13136070317323875No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA2100.12712326113539232No Hit
TGTAAAACGACGGCCAGTTCTATTGCTGAGACTTTCCAGAGCATTTTGCA2070.12530721454774388No Hit
TGTAAAACGACGGCCAGTACCTGAATCTTACTAAGCGCCTGGATCTATTG2050.1240965168226449No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC2030.12288581909754592No Hit
TGTAAAANGACGGCCAGTTGAAGAATGGTGGCTTGTTTCATGGTTTTTGT1960.1186483770596995No Hit
TGTAAAANGACGGCCAGTAGCCAAGATGGTGCCACTGCACTCCAGCCTGG1940.11743767933460054No Hit
TGTAAAANGACGGCCAGTCGCACAGTCTGTTCTGCAGCAACAACGGGATT1910.11562163274695207No Hit
TGTAAAANGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC1880.11380558615930361No Hit
TGTAAAANGACGGCCAGTCCCTGAAGGACAAGCCAGGCGTCTACACGAGA1810.10956814412145718No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG1810.10956814412145718No Hit
TGTAAAANGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC1770.10714674867125926No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC1720.1041200043585118No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC1710.10351465549596232No Hit
TGTAAAANGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG1710.10351465549596232No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)