FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003245933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159141
Sequences flagged as poor quality0
Sequence length300
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTTGCCCAACGTTGTAGTGTTGAGTGCATGGCAT67424.236494680817639No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAGTGCCAAGG43422.7283980872308202No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTGGTGGTCC41522.609007106905197No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA33852.1270445705380765No Hit
TGTAAAACGACGGCCAGTTGGAGTGCCTGGGTTAGAACCCAGGCATTCAA29431.8493034478858372No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC24131.5162654501354145No Hit
TGTAAAACGACGGCCAGTAGTCAGCCACGTTGGAACCAGTACTCACGCAA21911.3767665152286337No Hit
TGTAAAACGACGGCCAGTGGCTCCCCAGCTGTCTCTCTTGTAAAACGACG18871.1857409467076367No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCATAAACACAAA18631.1706599807717684No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA18491.161862750642512No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA18111.1379845545773875No Hit
TGTAAAACGACGGCCAGTACCGACTGGAGAGGAAGGCACTGGTGGTAGCT16901.0619513513173853No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGATCCCCGGTG16721.050640626865484No Hit
TGTAAAACGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT16501.0368164080909383No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA15840.9953437517673007No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC15680.985289774476722No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG15160.9526143482823409No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCCCTCGGAGCTG14650.9205672956686209No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG14420.9061147033134138No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC14190.8916621109582069No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA13810.8677839148930823No Hit
TGTAAAACGACGGCCAGTTGTCTGGTGGTGGAAGGAGGTGCACGCCACCA13730.8627569262477928No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG13180.8281963793114282No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTCACCATTTGGTG12680.7967777002783696No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC12130.7622171533420049No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAGGAGGACCC12090.7597036590193602No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT11680.733940342212252No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGCAGGATGGAG11550.7257714856636568No Hit
TGTAAAACGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT11320.7113188933084497No Hit
TGTAAAACGACGGCCAGTTCCTTCTTGGGTGGGTATTTTGCAACTGGGTA11250.7069202782438215No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG10370.6516234031456382No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC10200.6409410522743981No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC10140.6371708107904311No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT9890.6214614712739017No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC9880.6208330976932406No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGCCCCAATCCTGTCTC9770.6139209883059676No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC9700.6095223732413394No Hit
TGTAAAACGACGGCCAGTGCCATCGGCTCCCAAGTTTTACATCCTGGAGA9150.5749618263049748No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC8730.5485701359172055No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCAAGGAATTCAG8730.5485701359172055No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGGGCCCCGGGAGC8610.5410296529492714No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC7950.4995569966256339No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCAGGCCCCAAGGA7630.47944904204447625No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGTGGGAGTCTGGCTAC7540.4737936798185257No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCATAAACGCAAG7440.4675099440119139No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA7380.46373970252794694No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTCAGGCGGGAGG7350.4618545817859634No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTCCATTTGGTGTG7050.4430033743661282No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC6460.4059293331071188No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT6390.4015307180424906No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGAAGCCTCAACCC6290.3952469822358789No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC6280.3946186086552177No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAATTAGTAGT6210.39021999359058945No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGGCAGCACCTCT6200.3895916200099283No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCAGTTTCCTGCC6180.38833487284860596No Hit
TGTAAAACGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA6120.3845646313646389No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC6090.38267951062265537No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCCTGAGG5940.37325390691273774No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTTGTAAAACGAC5510.34623384294430726No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGCACATGGTT5440.341835227879679No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG5420.34057848071835667No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG5420.34057848071835667No Hit
TGTAAAACGACGGCCAGTCCGCCCTTTCCAAGCTCACTCTAGCCTGGGCA5410.3399501071376955No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTAGCCTCTCCATT5410.3399501071376955No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG5380.338064986395712No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGCACATGGGT5370.33743661281505083No Hit
TGTAAAACGACGGCCAGTCAGTTCCCAGGGACTCTGCTCTATGAGAGAGA5000.31418679033058733No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTCCTCACTCGGAT4820.3028760658786862No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG4780.3003625715560415No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCTCCTGCTCAGCCTT4720.2965923300720744No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGCCTGGGTGGGA4720.2965923300720744No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGGATGCTGAGTGGGAG4630.2909369678461239No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC4480.28151136413620625No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG4480.28151136413620625No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC4170.26203178313570985No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCTGCAGACAGGAT4100.2576331680710816No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC4050.25449130016777577No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGTGAACGAAG4050.25449130016777577No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC4000.2513494322644699No Hit
TGTAAAACGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG3980.2500926851031475No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCGGTCATAGCCTCT3900.24506569645785814No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC3890.24443732287719694No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCATAAACGCAAA3870.2431805757158746No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG3840.24129545497389107No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCTGTAAAACGACG3820.24003870781256872No Hit
TGTAAAACGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT3790.23815358707058523No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGGGTCAGTGGGAGTCT3760.23626846632860166No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGAAAGCCTCAGGCA3680.23124147768331227No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGTGGGGTCACCCC3680.23124147768331227No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG3650.22935635694132875No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCGGCTCACT3560.2237009947153782No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG3510.22055912681207235No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG3450.21678888532810528No Hit
TGTAAAACGACGGCCAGTTGAAGAGAGAGGTTAGCAAGGGAGGACTCCAG3450.21678888532810528No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTTCCTATCG3380.212390270263477No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCCGGCCCCAAGGA3370.21176189668281586No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCGTGACCGAGAGCT3330.20924840236017117No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCTGTGGGAGACCCAGC3300.20736328161818765No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCCCACAGACCCC3280.2061065344568653No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA3250.20422141371488176No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGGTTGGAGGTG3220.20233629297289826No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG3120.1960525571662865No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCATGTAAAACGACG3110.19542418358562533No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC3060.19228231568231946No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG3000.1885120741983524No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT2910.18285671197240183No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTGGGCCTGG2870.18034321764975714No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCAGGATGGAGGG2810.17657297616579007No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGGGCAGGCAGAATGT2730.17154598752050068No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCCCTCAGAGCTG2720.1709176139398395No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGCATCCTGGGTGAC2640.1658906252945501No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGTCATAGCTGTT2570.1614920102299219No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG2510.15772176874595484No Hit
TGTAAAACGACGGCCAGTTCCTGGGCTTCTCTTGAGTACGGAGGAAAGTG2420.15206640652000428No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCATAATCCCCCA2410.1514380329393431No Hit
TGTAAAACGACGGCCAGTAGTCAGCCACGTTGGAACCAGTGCTCACGCAA2390.15018128577802076No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGAGGCTTTTTGTTCCA2390.15018128577802076No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGTGGGGAGGCAG2340.14703941787471486No Hit
TGTAAAACGACGGCCAGTATCTCCTGGGGAGAAACGGCTTCTGTGTCCAA2290.14389754997140902No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCTGGCCCAAGTCA2250.1413840556487643No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA2150.13510031984215257No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCCCCCAGGATGG2140.1344719462614914No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGCTCAGGCCGTA2140.1344719462614914No Hit
TGTAAAACGACGGCCAGTCCTTGAAGGCTGGGCTTCTGTTTCCTGTTCTG2050.1288165840355408No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCCGCCCCAAGGAT2040.12818821045487963No Hit
TGTAAAACGACGGCCAGTGCCAATCCTGAGGCCATCAACACAAGGACTGG2040.12818821045487963No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGGGGGTGCCTCAA2010.1263030897128961No Hit
TGTAAAACGACGGCCAGTGGCTCTATATCAGGGCCCGCCGGTCATAGCTG1970.12378959539025142No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT1960.12316122180959024No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCCAGGGGGCGTCCCGG1910.12001935390628436No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGCGGAAGAGAGAGAC1900.11939098032562319No Hit
TGTAAAACGACGGCCAGTCCTTGAAGGCTGGGCTTCTGTCTTCTGGCAGG1900.11939098032562319No Hit
TGTAAAACGACGGCCAGTCCTTGAAGGCTGGGCTTCTGTCCAGTCCTTGA1780.1118504973576891No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACCTGGCGCAGGC1780.1118504973576891No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTTAATACATTAGT1760.11059375019636675No Hit
TGTAAAACGACGGCCAGTTGTCTGGTGGTGGAAGGAGGGGGTGGCTGCGC1760.11059375019636675No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTTGGCTTACT1740.10933700303504439No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAAGCCCCAGACCC1730.10870862945438321No Hit
TGTAAAACGACGGCCAGTCTGGGTTGGGGTGTCTGCTGTCTTCACTGTTG1730.10870862945438321No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGACCAGACG1720.10808025587372204No Hit
TGTAAAACGACGGCCAGTACCGACTGGAGAGGAAGGCAGTGCCTTGCCAG1670.10493838797041619No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGGG1650.10368164080909383No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACTGCCTTCCAGCATTT1650.10368164080909383No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGGAGGAGTGGTCATAG1630.10242489364777148No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCACCTTGGTCTCC1600.10053977290578794No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCCGTGGG1600.10053977290578794No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)