FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76413
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCATA17992.3543114391530238No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG16112.1082800047112404No Hit
CAGGAAACAGCTATGACCCGTAAAGGGCAAGGTGGGGTAATCTTGTCCAT15632.045463468258019No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA15592.040228756886917No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA15542.0336853676730398No Hit
CAGGAAACAGCTATGACCACCCCACCTGCTCAAAAGACACACTCCTAAAA12641.6541687932681612No Hit
CAGGAAACAGCTATGACCGTCCTAGCACCGCAGCGAATGGGACCCGCAAG12351.6162171358276733No Hit
CAGGAAACAGCTATGACCCCGTAGCCACGCCCCTATTGTCTGACCACGCT11771.5403138209466976No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA11631.5219923311478414No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG11081.450015049795192No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG10481.3714943792286654No Hit
CAGGAAACAGCTATGACCACCTGGCAGCCCCAAATCTGCACCTCCCGCAG10411.3623336343292372No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT9571.2524046955361No Hit
CAGGAAACAGCTATGACCGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT9551.249787339850549No Hit
CAGGAAACAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG9251.2105270045672858No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT9081.188279481240103No Hit
CAGGAAACAGCTATGACCTGGGCCTGGACACTTTGACGGCGGGGCACGTC8871.1607972465418188No Hit
CAGGAAACAGCTATGACCTGCTGGGAGAGGGGGATGAGCGGCGCCGCCAT8011.0482509520631307No Hit
CAGGAAACAGCTATGACCAGGACTTGCCCACTCTCCCAGTTACTTGGAAT7831.0246947508931725No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT7811.0220773952076216No Hit
CAGGAAACAGCTATGACCAGATCCTGTGAAGTGGGAGGGTTTTCAAAGTC7590.9932864826665619No Hit
CAGGAAACAGCTATGACCTGGAAACCCATGCCATGCACTCAACACTACAA7520.984125737767134No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT7520.984125737767134No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG7500.9815083820815829No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCACGGGCATCCTG7500.9815083820815829No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT7250.9487914360121968No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT6870.8990616779867301No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACCCTGTCTCTCTAGC6860.8977530001439545No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT6840.8951356444584037No Hit
CAGGAAACAGCTATGACCCTGGGTGCCGGGTAGAGTGTATACAGGGCTCA6800.8899009330873019No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC5950.7786633164513892No Hit
CAGGAAACAGCTATGACCGGAGAAGCACAGCTTCGGCTCAGGTACTGCCT5870.7681938937091857No Hit
CAGGAAACAGCTATGACCTCTGACTGTCCGTGATCCCACACAATCACCCT5680.7433290146964522No Hit
CAGGAAACAGCTATGACCGCCCCTACTCCAAAAGGCGGATGAATGGGGAA5580.7302422362686977No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC5570.7289335584259222No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC5550.7263162027403713No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACGGGGATGGCAGTG5500.7197728135264942No Hit
CAGGAAACAGCTATGACCAGGTTGCTGGATTCAGTTTGCTCAGGAAACAG5140.6726604111865782No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAATGAAACTTGGTT5100.6674256998154765No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT5060.6621909884443746No Hit
CAGGAAACAGCTATGACCGCTAATGCTTACTCTTTGCGTGAGTACTGGTT4930.6451781764882938No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA4510.5902137070917252No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG4390.5745095729784199No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGTTCTCCTTCCCT4300.5627314723934409No Hit
CAGGAAACAGCTATGACCGGAGAAGCACAGCTTCGGCTCTGGCCCTGGGG4260.5574967610223391No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCCAGCTAAGTGA4110.5378665933807074No Hit
CAGGAAACAGCTATGACCTGACTTTTGCAAGTCACAGAGAACTGTTGTAT4010.524779814952953No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCTGAATAGTTGT3890.5090756808396477No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGGACACTGCCCAC3740.489445513198016No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC3520.4606546006569563No Hit
CAGGAAACAGCTATGACCGGGGAATGGGAAGGGAGATGAAAGAGTAGGTG3480.4554198892858545No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCTGGGAAAAAGGG3390.4436417887008755No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT3040.39783806420373496No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT3040.39783806420373496No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCAGAGAGTGATAT2900.37951657440487874No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCTGAGCAGTGGGG2890.3782078965621033No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCTGCCCAACACCC2790.36512111813434883No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCCAGAGGGGTC2790.36512111813434883No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTTGTAATCCCATC2770.362503762448798No Hit
CAGGAAACAGCTATGACCTCCCTTAAACTTGGGATTGCTCCATTGCACTC2740.3585777289204717No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTGATCCCCCAAAA2710.3546516953921453No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGGCACGGGAACA2680.350725661863819No Hit
CAGGAAACAGCTATGACCTTGGACAGCACCTTGAATGCCTGGGTTCTAAC2650.34679962833549266No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTCCATTCT2620.34287359480716634No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTACTCCCAACCA2450.3206260714799838No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCAAGTGCTGATGTA2440.31931739363720835No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT2390.3127740044233311No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCACGGTGGCTCAT2320.303613259523903No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC2290.2996872259955767No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGACACTGCCCACC2280.29837854815280124No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTTCATTCT2190.2866004475678222No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC2170.28398309188227133No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTATTCCCAACCA2130.27874838051116957No Hit
CAGGAAACAGCTATGACCGCAGCAAGAGACCAATACCGCATTGTAGTCAA2110.2761310248256187No Hit
CAGGAAACAGCTATGACCTGCTTCAGTCGCCTGCACAACAGTGAAGACAG2090.2735136691400678No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC2070.27089631345451687No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAATAGTCT2070.27089631345451687No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCAGGTCCCGGGA1980.2591182128695379No Hit
CAGGAAACAGCTATGACCTCCAGCCAGCCAGTCCTTGTAACTCAGCATGA1950.2551921793412116No Hit
CAGGAAACAGCTATGACCCTCACCAAGAGTGCCCCACGTGGTGGGCCACA1950.2551921793412116No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC1930.2525748236556607No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCGGGTCTTACA1810.23687068954235535No Hit
CAGGAAACAGCTATGACCGATGGCAGAATGGAAACATGGCAAAAGAAGAG1780.23294465601402903No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1770.2316359781712536No Hit
CAGGAAACAGCTATGACCACCCAGTTAGAGCCATGGTTCCACTAGACTCT1680.21985787758627456No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1620.21200581052962195No Hit
CAGGAAACAGCTATGACCACTCCCTTGAGCCTTTGGGGAAAGGGAGACAG1620.21200581052962195No Hit
CAGGAAACAGCTATGACCGATGGCAGAATGGAAACATGGCAAAAAAGAGG1620.21200581052962195No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACACAGCTGGGAGGC1400.18321489798856214No Hit
CAGGAAACAGCTATGACCCCTGACCCCTTCTTCTCCAGGAAACTCTGTTC1350.17667150877468493No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCAGAGCCACC1300.1701281195608077No Hit
CAGGAAACAGCTATGACCAACACGGCAGTCAGAGGTGCAGGGGATGAGGT1280.16751076387525682No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT1260.16489340818970594No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTTGTAGTCCCAGC1220.15965869681860417No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGGGCGCATGTAAT1160.1518066297619515No Hit
CAGGAAACAGCTATGACCGGAAATAAGTACCTTCTGTGTCCTTGTACGTT1150.15049795191917606No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCATGTGTTCAGC1130.14788059623362515No Hit
CAGGAAACAGCTATGACCTGCTGGGAGAGGGGGATGAGTAGACAGGGCCA1120.14657191839084974No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACCACCGTGATCTCTC1090.1426458848625234No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTTCTTCCAAACA1090.1426458848625234No Hit
CAGGAAACAGCTATGACCACTCCCTTGAGCCTTTGGGGGCCACTGCAGCT1050.13741117349142162No Hit
CAGGAAACAGCTATGACCCCTGACCCCTTCTTCTCCAGGAAACTCTCTTC1040.13610249564864618No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC1030.1347938178058707No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACCAGACCAATGCC970.12694175074921807No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCATGTGTTCAGT940.12301571722089175No Hit
CAGGAAACAGCTATGACCGGCGGGCCCTGATATAGAGCAGGCGCCGCGGG910.11908968369256541No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGAAGCTCCAGCA900.11778100584978995No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC880.11516365016423907No Hit
CAGGAAACAGCTATGACCAACTTTTAGGTAGAGCCAAAGAACTGGCCGTC880.11516365016423907No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA870.11385497232146362No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG840.10992893879313728No Hit
CAGGAAACAGCTATGACCTGAGTTAACGTGATTTAGGACCCTATGTGACT800.10469422742203552No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCTGGGCAGAAGGG800.10469422742203552No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG790.10338554957926008No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)