FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138590
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC41332.9821776462948266No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG34422.483584674218919No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA31682.2858792120643625No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA29452.1249729417706904No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG25091.8103759289992063No Hit
TGTAAAACGACGGCCAGTCCAGAGATCAATAATAGCTCAGGGGAGATGGA23651.706472328450826No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT22431.6184428890973375No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC20871.5058806551699258No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT20101.4503210909878057No Hit
TGTAAAACGACGGCCAGTGAGCTGCTGACGCACTGTGACTCCAGCCAGAG19741.4243451908507108No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG18991.3702287322317628No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC17081.232412150948842No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTGGTGGTCC16761.2093224619380907No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC16541.1934483007431993No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC15931.149433581066455No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGCCCCAATCCTGTCTC15481.1169637058950863No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC15411.1119128364239843No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCTGAGTAGCCACAG14481.0448084277364889No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC14401.0390360054838013No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT14101.0173894220362218No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG13941.0058445775308464No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG13911.0036799191860883No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT13650.9849195468648532No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA13040.9409048271881089No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG12910.9315246410274912No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC12590.9084349520167401No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT12200.880294393534887No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC11130.8030882459051879No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA11040.7965942708709142No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG10950.7901002958366404No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG10540.7605166317916155No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC10340.7460855761598961No Hit
TGTAAAACGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA10280.7417562594703803No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACCTGGCGCAGGC9530.6876398008514323No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT9480.6840320369435023No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGGTTGGAGGTG9480.6840320369435023No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC9350.6746518507828847No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTGACCCTTCCAGGA9330.6732087452197129No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA8880.6407388700483441No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCAAGGGT8830.6371311061404141No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC8700.6277509199797965No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCATGTAAAACGACG8600.6205353921639367No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGCAGGATGGAG8530.615484522692835No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG8370.6039396781874594No Hit
TGTAAAACGACGGCCAGTCAGTTCCCAGGGACTCTGCTCTATGAGAGAGA8220.5931163864636698No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC8150.5880655169925679No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT7820.5642542752002309No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG7540.5440507973158236No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG7290.5260119777761744No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA6990.5043653943285952No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTAGAGCCTGGCT6410.46251533299660874No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC6310.455299805180749No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCAACCGT6310.455299805180749No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGGTCTGTGCGG6210.4480842773648892No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG6210.4480842773648892No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCACCGAGGCAG5870.4235514827909662No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA5850.42210837722779415No Hit
TGTAAAACGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG5170.37304278807994806No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG5060.3651057074825023No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC5050.3643841547009164No Hit
TGTAAAACGACGGCCAGTGCCCAAGGTTGGTGAGATGGGAACCACCCAGC4700.3391298073454073No Hit
TGTAAAACGACGGCCAGTAGGTGGCAAAGCTCAATCAGGACAGTTCTGAA4670.3369651490006494No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGACCTTGGTAGGAT4610.3326358323111336No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG4440.32036943502417203No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGGG4330.31243235442672634No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG4320.31171080164514037No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCATAGCTGTTTC4280.30882459051879646No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTTGTAAAACGAC4100.29583664045024893No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCAAGCCG4070.293671982105491No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCAGTGGG4040.29150732376073313No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGTGTAAAACGACG3980.28717800707121727No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCAGTTTCCTGCC3980.28717800707121727No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG3970.2864564542896313No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGTCAAAGGCAGGA3960.2857349015080453No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG3950.28501334872645934No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCAGCCCCTGCCA3920.2828486903817014No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGTGAACGAAG3910.28212713760011543No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT3860.2785193736921856No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGCCTGGGTGGGA3640.26264521249729417No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTACACTTAG3610.2604805541525363No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCTGGAGGGCGGCCA3500.25254347355509055No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCTGCAGACAGGAT3340.240998629049715No Hit
TGTAAAACGACGGCCAGTGCCATCGGCTCCCAAGTTTTATGTCCTAGAGC3220.2323399956706833No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCAATCATCTAGG3150.22728912619958153No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCGCAGCCCTCCTAC3080.2222382567284797No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC3060.22079515116530773No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCACCTTGGCCTCC2910.20997185944151817No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC2900.2092503066599322No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCATGTGAAGAGAG2830.20419943718883038No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGGAGCTGTGTGT2770.19987012049931452No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCTGGAGGGCAGCCA2760.19914856771772854No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC2690.19409769824662676No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGCCGGTGCCCAT2640.19048993433869688No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCGCCTCGGCCTCC2590.186882170430767No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCATAGCTGTT2570.18543906486759507No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCCCAGTCACCAGC2220.16018471751208602No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT2200.15874161194891406No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCCGTGGG2150.15513384804098418No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC1990.14358900353560863No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG1980.14286745075402266No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCACGTGTCGCCC1970.14214589797243668No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGAGGCTCCTTCC1960.1414243451908507No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCACCTCAGCCTCC1960.1414243451908507No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGTCCCAGGTACT1820.13132260624864708No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGAGGGAGGATCC1750.12627173677754527No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTTGGACCCAGAG1750.12627173677754527No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGGGCAGGTGCT1740.1255501839959593No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCTGCACAACTGC1720.12410707843278736No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTTCCTATCG1720.12410707843278736No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCAGGATGGAGGG1720.12410707843278736No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAGCC1680.12122086730644346No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTAGGATTTTTTTTT1670.1204993145248575No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTTAGTATTTGAA1640.11833465618009957No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCACCTCGGCCTCT1590.11472689227216971No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCACAGCTGT1590.11472689227216971No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC1530.11039757558265387No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCAGGAAATCAGGCT1480.106789811674724No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTACCCAGTGGTGTG1460.10534670611155206No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGACAGATGCCCC1440.1039036005483801No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCATCACCAGG1410.1017389422036222No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGCAATCCC1390.10029583664045025No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)