FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138590
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT32872.3717439930730935No Hit
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG29692.142290208528754No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC29282.112706544483729No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT28872.083122880438704No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT28662.0679702720253985No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA23511.6963705895086227No Hit
CAGGAAACAGCTATGACCGGGGAGACTGGGCCATACGATAGGCTTAATCC21851.576592827765351No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA20981.5138177357673714No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC20451.4755754383433148No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC19341.395483079587272No Hit
CAGGAAACAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT18021.3002381124179234No Hit
CAGGAAACAGCTATGACCAGCCTCTCCCCTCGTTCCAGCAACTCAGTGAG17831.2865286095677897No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT17551.2663251316833828No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC17141.2367414676383577No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG16721.2064362508117468No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC16441.1862327729273396No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCTGAGGCAGGAGA15631.1277869976188757No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGGCACGGGAACA14811.068619669528826No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCTGGGTGCTGGTG14411.039757558265387No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC13250.9560574356014142No Hit
CAGGAAACAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT12160.8774081824085431No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCAGGAAACAGCT12030.8680279962479255No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC10840.7821632152391949No Hit
CAGGAAACAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG10750.775669240204921No Hit
CAGGAAACAGCTATGACCTCCCTTAAACTTGGGATTGCTCCATTGCACTC10530.7597950790100296No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGCAGGAAACAGCT10440.7533011039757559No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCCCGTTAGCTGC10330.7453640233783101No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC10250.7395916011256223No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT10240.7388700483440364No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT10240.7388700483440364No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGTCTGGGTCCT9750.7035139620463237No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT9730.7020708564831517No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA9610.6934122231041201No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCCTGCCCAGGCC9400.6782596146908146No Hit
CAGGAAACAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG9190.6631070062775092No Hit
CAGGAAACAGCTATGACCTGAAGTGGCTCCATCCTTCCATTTGTTTATAT9170.6616639007143372No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG9030.6515621617721336No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA8420.6075474420953892No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT8070.5822930947398802No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC7830.5649758279818169No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG7660.5527094306948553No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT7580.5469370084421675No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT7490.5404430334078938No Hit
CAGGAAACAGCTATGACCTGCCCAGTTGTTTCCAGAACCTTGTTTTTGAC7470.5389999278447218No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT6980.5036438415470091No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT6780.48921278591528966No Hit
CAGGAAACAGCTATGACCGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC6260.4516920412728191No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC6130.44231185511220145No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGTTGAGCTCCTCA6070.43798253842268564No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCACAGACCCCACA5920.427159246698896No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA5920.427159246698896No Hit
CAGGAAACAGCTATGACCTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG5740.41417129663034846No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCAGGGCAGCGGT5730.41344974384876254No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCAGGAAACAGCT5540.39974024099862904No Hit
CAGGAAACAGCTATGACCCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG5300.3824229742405657No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA4550.3283065156216177No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCTAGCTGCCTATC4500.3246987517136879No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTTTAGG4460.32181254058734393No Hit
CAGGAAACAGCTATGACCACCCCTTGCATTGGTAGGGCTTCTCTCCTGTG4430.31964788224258606No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGACCATGGCTTAC4230.3052168266108666No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCAGACACACGGC4150.2994444043581788No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCACTGGCCGTCGTT3950.28501334872645934No Hit
CAGGAAACAGCTATGACCGGGCAGGCCGAGGACATTAGAAATTTGGGACG3940.28429179594487336No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAATGAAACTTGGTT3910.28212713760011543No Hit
CAGGAAACAGCTATGACCGGGTCCCACCCATGCTGAAAGTCCACCTTCCA3810.2749116097842557No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGCTGGGGACTGGA3790.27346850422108376No Hit
CAGGAAACAGCTATGACCCCGTAGCCACGCCCCTATTGTCTGACCACGCT3730.26913918753156796No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGCTTGAGCTCCTC3670.2648098708420521No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTGACACA3560.2568727902446064No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC3500.25254347355509055No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGACAGGAAACAGCT3500.25254347355509055No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAAGAAGTCTTTTAA3490.25182192077350457No Hit
CAGGAAACAGCTATGACCGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT3470.2503788152103326No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGAAAAGTTTCAGG3420.2467710513024028No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC3370.2431632873944729No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGTCTGCAGCCTGG3300.2381124179233711No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC3260.23522620679702722No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTCCATTCT3220.2323399956706833No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG3220.2323399956706833No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCCAAACCAG3210.23161844288909733No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTTCATTCT3180.22945378454433943No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG3110.22440291507323762No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACTAGAAGTAATTA3090.22295980951006567No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGACAGGGCTGACTG3090.22295980951006567No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCACTGGTCGTCGTT3070.22151670394689374No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCAAGTGCTGATGTA3070.22151670394689374No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCATGTGTTCAGT3030.2186304928205498No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG2950.21285807056786202No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCTATTTTCCAAAG2870.20708564831517423No Hit
CAGGAAACAGCTATGACCGCAGCCACCCTGAACACACTTGGCTATCACCC2750.19842701493614257No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC2610.18832527599393897No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCAGCCACCACCACGTACCACT2610.18832527599393897No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTCACACCTGTAA2600.187603723212353No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTACTCCCAACCA2570.18543906486759507No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC2530.18255285374125116No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACACAGCTCCTGCC2460.17750198427014935No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCACGGTGGCTCAT2420.17461577314380547No Hit
CAGGAAACAGCTATGACCTCCTGCCCCACCCTGATACAATATAGTCTCCC2410.1738942203622195No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG2340.16884335089111768No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCGGGTCTTACA2150.15513384804098418No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGGGGGTGGATC2040.1471967674435385No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCCTCCCAGGTTCA2040.1471967674435385No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTATTCCCAACCA2010.14503210909878056No Hit
CAGGAAACAGCTATGACCGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG2000.1443105563171946No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCCTCTCCCAACT1990.14358900353560863No Hit
CAGGAAACAGCTATGACCGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG1920.13853813406450682No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCCCTCCGGGCA1850.13348726459340501No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTTTGGGAGGCTG1800.12987950068547516No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGTGAGGACAGAT1770.12771484234071723No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTATAATCCCAGC1770.12771484234071723No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTCACGCCTGTAA1750.12627173677754527No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCCTGGCTTGTC1710.1233855256512014No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCTAAACCAG1700.1226639728696154No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCTGCCCCTCCCCA1620.11689155061692764No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTTTCAGAGATGGT1610.11616999783534165No Hit
CAGGAAACAGCTATGACCCCTGACCCCTTCTTCTCCAGGAAACTCTCTTC1570.11328378670899776No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGATGCAGTCAAGAG1540.11111912836423984No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCAGAGAGTGATAT1510.10895447001948193No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG1500.10823291723789595No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCCTGGGTGTCTG1440.1039036005483801No Hit
CAGGAAACAGCTATGACCTCCCTTAAACTTGGGATTGCTCCAGCCTGTGC1430.10318204776679414No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCCAATTCCTCCCC1420.10246049498520816No Hit
CAGGAAACAGCTATGACCCCTGACCCCTTCTTCTCCAGGAAACTCTGTTC1400.10101738942203622No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)