FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153520
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCAGTGGTGCGATG124248.09275664408546No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGTCATAGCTGTT95336.209614382490881No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA52833.441245440333507No Hit
TGTAAAACGACGGCCAGTTCCCAAAGGCTGGTGGAGGAGATGCTGAAGAT36552.3807972902553414No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC34662.257686294945284No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA34032.2166492965085984No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC32102.090932777488275No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA29971.9521886399166233No Hit
TGTAAAACGACGGCCAGTAGATCATCCAGCGCACACGGTGTAAAACGACG29121.8968212610734756No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT23811.5509379885356955No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG22221.4473684210526316No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGATGGCACTGA21961.430432516935904No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC21631.4089369463262116No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA21291.3867899947889526No Hit
TGTAAAACGACGGCCAGTTGCCCAACGTTGTAGTGTTGAGTGCATGGCAT19291.2565138092756645No Hit
TGTAAAACGACGGCCAGTCCAGAGATCAATAATAGCTCAGGGGAGATGGA15761.0265763418447107No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC14220.9262636789994789No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCAAGGGT13220.8611255862428348No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTGGTGGTCC13070.8513548723293383No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC12670.8252996352266806No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA12100.7881709223553934No Hit
TGTAAAACGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT11900.7751433038040646No Hit
TGTAAAACGACGGCCAGTAGATCATCCAGCGCACACGGCAGGAGACAGGC11000.716519020323085No Hit
TGTAAAACGACGGCCAGTAGTGACAACAGCCCCAAAGGAATGATCCCGCT10650.6937206878582595No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT10650.6937206878582595No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG10480.68264721208963No Hit
TGTAAAACGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC8800.5732152162584679No Hit
TGTAAAACGACGGCCAGTGTTCTACCGTCAGTCCTGGCATTATTTCTCTC8630.5621417404898384No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG7670.4996091714434601No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT7310.4761594580510683No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT6480.42209484106305367No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC6300.41036998436685773No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGGGAGGATCCTC5930.38626889004689946No Hit
TGTAAAACGACGGCCAGTCGCCTGAATAGGTGCAGGGGCACGATATCCAT5900.3843147472642001No Hit
TGTAAAACGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG5580.36347055758207397No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG5310.3458832725377801No Hit
TGTAAAACGACGGCCAGTCAGTTCCCAGGGACTCTGCTCTATGAGAGAGA5070.33025013027618555No Hit
TGTAAAACGACGGCCAGTAGGTCATCCTCCTGCCTCGGCCTCCCAAAGTG4670.3041948931735279No Hit
TGTAAAACGACGGCCAGTCCTGGCCCACAAACTGTGCTGGGGTCATAGCT4510.2937727983324648No Hit
TGTAAAACGACGGCCAGTTGACACAGCTGCTTCTGAGTACAAGTATGTAG3780.24622199062011466No Hit
TGTAAAACGACGGCTAGTGTCCCAGGTGTATGGAGCGCAGTGGTGCGATG3650.23775403856175092No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGTGACC3500.2279833246482543No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAGCC3490.22733194372068785No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGCGTTGGTCATAGCTGTT3460.22537780093798854No Hit
TGTAAAACGACGGCCAGTCGCCTGAATAGGTGCAGGGGTGCGATTATGGC3440.22407503908285567No Hit
TGTAAAACGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCAGGTGTA3200.20844189682126105No Hit
TGTAAAACGACGGCCAGTTGAAGAATGGTGGCTTGTTTCATGGTGGCCTT3030.19736842105263158No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCCTGCCTGTCCCAGG2990.19476289734236582No Hit
TGNAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGTAGTGGTGCGATG2980.19411151641479937No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG2910.18955184992183427No Hit
TGTAAAACGACGGCTAGTGAGGGAGGCCAAGGGTGTTGGTCATAGCTGTT2860.1862949452840021No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGCA2820.18368942157373633No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA2800.18238665971860343No Hit
TGTAAAACGACGGCCAGTATCTCCTGGGGAGAAACGGCTTCTGTGTCCAA2790.18173527879103699No Hit
TGTAAAACGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA2590.16870766023970818No Hit
TGTAAAACGACGGCCAGTCGCCTGAATAGGTGCAGGGGCACGAGCTCGGC2390.15568004168837937No Hit
TGTAAAACGACGGCCAGTTGAAGAGAGAGGTTAGCAAGGGACAGGAGGGA2280.1485148514851485No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCTCCCG2250.1465607087024492No Hit
TGTAAAACGACGGCCAGTCCTTGAAGGCTGGGCTTCTGTGTAAAACGACG2210.14395518499218343No Hit
TGNAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGTCATAGCTGTT2210.14395518499218343No Hit
TGTAAAACGACGGCCAGTTGAAGAATGGTGGCTTGTTTCATGGTGTAAAA2120.13809275664408546No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCTCAGCCTCCTGCTCA2100.1367899947889526No Hit
TGTAAAACGACGGCCAGTTCCTGGGCTTCTCTTGAGTACGGAGGAAAGTG2050.1335330901511204No Hit
TGTAAAACGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG1980.12897342365815528No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTA1970.12832204273058884No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG1930.12571651902032308No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGAGGATCCTCCCT1890.12311099531005733No Hit
TGTAAAACGACGGCCAGTCGCCTGAATAGGTGCAGGGGCACAGTCATAGT1870.12180823345492443No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGAAACC1860.121156852527358No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGTAACC1860.121156852527358No Hit
TGTAAAACGACGGCCAGTATTCCTCCACTATCGCCCCTGGACGTCCCGTC1850.12050547159979155No Hit
TGTAAAACGACGGCCAGTTGAAGAGAGAGGTTAGCAAGGGAGAACCCATT1810.11789994788952579No Hit
TGTAAAACGACGGCCAGTTGAAGAGAGAGGTTAGCAAGGGAAACACTGCA1620.10552371026576342No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCACCGCCACACT1610.10487232933819698No Hit
TGTAAAACGACGGCCAGTCGCCTGAATAGGTGCAGGGGGACAGGGTCTTG1600.10422094841063052No Hit
TGTAAAACGACGGCCAGTAGATCATCCAGCGCACACGGAGACAGGCCCAG1590.10356956748306409No Hit
TGTAAAACGACGGCCAGTTGAAGAGAGAGGTTAGCAAGGGAACGACCGCC1570.10226680562793122No Hit
TGTAAAACGACGGCCAGTTGAAGAGAGAGGTTAGCAAGGGACCTTCTCCG1570.10226680562793122No Hit
TGTAAAACGACGGCCAGTACACATTCTTGTAAAGCATGGGATTGCTGAAT1560.10161542470036478No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)