FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153520
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNATCGCACCACTGCACTCCAGCAGGAAACAGCT41112.677826993225638No Hit
CAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT31192.031657113079729No Hit
CAGGAAANAGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT28721.8707660239708181No Hit
CAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG23481.529442417926003No Hit
CAGGAAANAGCTATGANNATCGCACCACTGCGCTCCATACACCTGGGACA19961.3001563314226159No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC19031.2395779051589368No Hit
CAGGAAANAGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC15701.0226680562793122No Hit
CAGGAAANAGCTATGANNAACACGGCAGTCAGAGGTGCAGGAAACAGCTA15100.9835852006253257No Hit
CAGGAAANNGCTATGANNATCGCACCACTGCACTCCAGCAGGAAACAGCT14540.9471078686816049No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT13230.8617769671704013No Hit
CAGGAAANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT12110.7888223032829598No Hit
CAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT10910.710656591974987No Hit
CAGGAAANNGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT10760.7008858780614903No Hit
CAGGAAANAGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA9490.6181605002605524No Hit
CAGGAAANAGCTATGANNACCTCTCTGGGCCTGTCTCCTGGCCGTCGTTT9390.611646690984888No Hit
CAGGAAANAGCTATGANNAACACCCTTGGCCTCCCTCACTGGCCGTCGTT9050.589499739447629No Hit
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC8830.5751693590411673No Hit
CAGGAAANNGCTATGANNATCGCACCACTGCGCTCCATACACCTGGGACA8510.5543251693590412No Hit
CAGGAAANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG8160.5315268368942158No Hit
CAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC7130.46443460135487236No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT6680.4351224596143825No Hit
CAGGAAANAGCTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC5940.3869202709744659No Hit
CAGGAAANNGCTATGANNAACACGGCAGTCAGAGGTGCAGGAAACAGCTA5900.3843147472642001No Hit
CAGGAAANAGCTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA5860.38170922355393433No Hit
CAGGAAANAGCTATGANNCCTTGGGCAAGTTAACAAACCTCTCTGAGCCT5840.3804064616988015No Hit
CAGGAAANNGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC5440.3543512245961438No Hit
CAGGAAANAGCTATGANNCTTGAGCTCCTCGGACACGGCAGGAAACAGCT4830.3146169880145909No Hit
NAGGAAANNGCTATGANNATCGCACCACTGCACTCCAGCAGGAAACAGCT4710.3068004168837936No Hit
CAGGAAANAGCTATGANNCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC4590.29898384575299636No Hit
CAGGAAANAGCTATGANNCTGGCCTCCTTCAATGCATTTAATGGCCTTCT4490.29247003647733194No Hit
CAGGAAANAGCTATGANNACCTCTCTGGGCCTGTCTCCTGCCGTGTGCGC4490.29247003647733194No Hit
CAGGAAANAGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT4480.2918186555497655No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT4460.2905158936946326No Hit
CAGGAAANNGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT4370.28465346534653463No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC4300.2800937988535696No Hit
CAGGAAANAGCTATGANNATCGCACCACTGCACTCCAGGAAACAGCTATG4270.2781396560708702No Hit
CAGGAAANAGCTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT4260.2774882751433038No Hit
CAGGANANNGCTATGANNATCGCACCACTGCACTCCAGCAGGAAACAGCT4190.27292860865033874No Hit
CAGGAAANAGCTATGANNTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG4120.2683689421573736No Hit
CAGGAAANAGCTATGANNCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT4110.2677175612298072No Hit
NAGGANANNGCTATGANNATCGCACCACTGCACTCCAGCAGGAAACAGCT4090.2664147993746743No Hit
CAGGAAANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC3940.2566440854611777No Hit
CAGGAAANNGCTATGANNAACACCCTTGGCCTCCCTCACTGGCCGTCGTT3910.2546899426784784No Hit
CAGGAAANAGCTATGANNAGAGACCAGATGCATAAGCACAGCAGCATTCC3830.24947889525794684No Hit
NAGGANANNGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT3520.22928608650338717No Hit
CAGGAAANNGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA3510.22863470557582072No Hit
NAGGANANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT3500.2279833246482543No Hit
CAGGAAANNGCTATGANNACCTCTCTGGGCCTGTCTCCTGGCCGTCGTTT3480.22668056279312143No Hit
NAGGAAANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT3460.22537780093798854No Hit
CAGGAAANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT3450.22472642001042212No Hit
CAGGANANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT3420.22277227722772275No Hit
CAGGAAANAGCTATGANNTGCTGCCGAGAGAGGACAGTCCCCAACCATAG3350.2182126107347577No Hit
NAGGAAANNGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT3300.21495570609692546No Hit
CAGGAAANAGCTATGANNCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG3290.21430432516935904No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC3070.19997394476289734No Hit
CAGGANANNGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT3040.19801980198019803No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC2830.18434080250130275No Hit
CAGGAAANNGCTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA2700.17587285044293904No Hit
NAGGAAANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2550.16610213652944242No Hit
CAGGANANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2530.16479937467430955No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC2530.16479937467430955No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTATGTGCA2520.16414799374674308No Hit
CAGGANANNGCTATGANNATCGCACCACTGCGCTCCATACACCTGGGACA2490.1621938509640438No Hit
CAGGAAANAGCTATGANNTGCTGCTTTTCCTGCCAGAAGCCCAGCCTTCA2440.15893694632621158No Hit
CAGGAAACAGCNATGANNATCGCACCACTGCACTCCAGCAGGAAACAGCT2410.15698280354351224No Hit
CAGGAAANNGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT2370.1543772798332465No Hit
NAGGAAANNGCTATGANNATCGCACCACTGCGCTCCATACACCTGGGACA2360.15372589890568003No Hit
NAGGANANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2340.15242313705054716No Hit
CAGGAAANAGCTATGANNGCATCACTGAGGGAGGATCCTCCCCGGGCAGA2320.1511203751954143No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT2270.1478634705575821No Hit
CAGGAAANAGCTATGANNGGAGGCTGAGGTGGGAGGATCACTTGAGCCTG2260.14721208963001564No Hit
CAGGAAANAGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA2240.14590932777488275No Hit
NAGGANANNGCTATGANNATCGCACCACTGCGCTCCATACACCTGGGACA2230.1452579468473163No Hit
NAGGAAANAGCTATGANCATCGCACCACTGCACTCCAGCAGGAAACAGCT2200.14330380406461699No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG2190.14265242313705054No Hit
NAGGAAANAGCTATGANNATCGCACCACTGCACTCCAGCAGGAAACAGCT2160.14069828035435122No Hit
CAGGAAACAGCTATGANCATCGCACCACTGCACTCCAGCAGGAAACAGCT2090.13613861386138612No Hit
CAGGAAANAGCTATGANNCTTGAGCTCCTCAGCCACCACCACGTACCACT2080.1354872329338197No Hit
NAGGANANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2070.13483585200625325No Hit
NAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2050.1335330901511204No Hit
CAGGAAACNGCTATGANNATCGCACCACTGCACTCCAGCAGGAAACAGCT2050.1335330901511204No Hit
CAGGAAANAGCTATGANNGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG2040.13288170922355394No Hit
CAGGANANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2040.13288170922355394No Hit
CAGGAAACAGCNATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2040.13288170922355394No Hit
CAGGAAANAGCTATGANNCCAGCAGAGCTCTTCTTACAGAGGCAGGAAAC2030.1322303282959875No Hit
CAGGAAANAGCTATGANNTGCCCAGTTGTTTCCAGAACCTTGTTTTTGAC2000.13027618551328818No Hit
CAGGAAANAGCTATGANNAGCGTCTCATTCCATAAAGGCCACCATTCTTC1990.12962480458572173No Hit
CAGGAAANNGCTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC1980.12897342365815528No Hit
CAGGAANNNGCTATGANNATCGCACCACTGCACTCCAGCAGGAAACAGCT1910.1244137571651902No Hit
NAGGAAANAGCTATGANCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1900.12376237623762376No Hit
CAGGAAANNGCTATGANNTGCTGCCGAGAGAGGACAGTCCCCAACCATAG1900.12376237623762376No Hit
CAGGAAANNGCTATGANNCCTTGGGCAAGTTAACAAACCTCTCTGAGCCT1850.12050547159979155No Hit
CAGGAAANNGCTATGANNACCTCTCTGGGCCTGTCTCCTGCCGTGTGCGC1840.11985409067222512No Hit
CAGGANANNGCTATGANNAACACGGCAGTCAGAGGTGCAGGAAACAGCTA1800.11724856696195936No Hit
NAGGAAANNGCTATGANNAACACGGCAGTCAGAGGTGCAGGAAACAGCTA1790.11659718603439291No Hit
CAGGAAANAGCTATGANNTGACAGCACAAGGAACACTGACCTACAGATAA1760.11464304325169358No Hit
CAGGAAACNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1760.11464304325169358No Hit
NAGGAAANAGCTATGANCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1760.11464304325169358No Hit
NAGGAAANNGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC1730.11268890046899427No Hit
CAGGAANNNGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1730.11268890046899427No Hit
CAGGAAACAGCTATGANCAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1700.11073475768629494No Hit
CAGGAANNNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1690.11008337675872851No Hit
CAGGAAANNGCTATGANNCTTGAGCTCCTCGGACACGGCAGGAAACAGCT1670.10878061490359563No Hit
CAGGAAANNGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC1660.1081292339760292No Hit
CAGGAAANAGCTATGANNAGCGTCTCATTCCATAAAGGCCACCATGAAAC1650.10747785304846273No Hit
NAGGANANNGCTATGANNAACACGGCAGTCAGAGGTGCAGGAAACAGCTA1640.1068264721208963No Hit
CAGGAAACAGCTATGANCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1640.1068264721208963No Hit
NAGGAAANAGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1610.10487232933819698No Hit
CAGGAAANAGCTATGANNGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT1610.10487232933819698No Hit
CAGGAAANNGCTATGANNCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC1610.10487232933819698No Hit
CAGGAAACAGCNATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1600.10422094841063052No Hit
NAGGANANNGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC1570.10226680562793122No Hit
CAGGAAACNGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1560.10161542470036478No Hit
CAGGAAANAGCTATGANNGGAGGCTGAGGTGGGAGGATCACTTGTGTCTG1560.10161542470036478No Hit
CAGGAAANAGCTATGANNCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA1550.10096404377279833No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)