FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003245957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256501
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC211838.258447335487972No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC158516.1797030031072No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG107184.178541214264272No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGCTACGGGGT103744.044428676691319No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG100173.9052479327565974No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG91033.5489140393214837No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC85903.3489148190455396No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC82283.2077847649716764No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGCCTCACAGGGTCATAGCTG66682.5996000015594483No Hit
TGTAAAACGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG65792.564902281082725No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG61832.4105169180626973No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCAAGGGT43451.6939505109141872No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCAGTGGG38291.492781704554758No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGTAAAACGACG37511.4623724663841466No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA32401.263152970163859No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC25630.999216377324065No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCAGTGGTGCGATG21490.8378134978031274No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCTGGAGGGCGGCCA20620.8038955013820608No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT19750.7699775049609943No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC19640.7656890226548825No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCTGGAGTGCAGTG18370.7161765451206819No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT18340.7150069590371968No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGTGAACGAAG17210.670952549892593No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGGCTACGGGGTCA17030.6639350333916827No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG15850.6179313141079372No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCAATAGGGGCGTGGCTACGGGGT14940.5824538695755572No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC14840.5785552492972736No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG14270.5563331137110576No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTTGGACCCAGAG13520.5270934616239312No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG12930.5040916019820586No Hit
TGTAAAACGACGGCCAGTGAGACAGGCTTCTGGGCTGTCCACAACTCTCT12810.49941325764811834No Hit
TGTAAAACGACGGCCAGTTGAGAGGGACTGGAGAAAGGGAACTTACAGAG12140.4732925017836188No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA9800.3820647872717845No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCAAGTAGCCACAG9230.35984265168556845No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGGGGGCGAGGG8560.3337218958210689No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGCGCCTG8500.3313827236540988No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCAGGCGCCTG7890.30760113995656935No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT7010.27329328150767446No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC6970.27173383339636104No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGTGCCTG6640.25886838647802546No Hit
TGTAAAACGACGGCCAGTGGGGTGGGGAGTGAGATGGTCATAGCTGTTTC6080.23703611291963772No Hit
TGTAAAACGACGGCCAGTACATGCGTGGCCCTGTCTACGGCCCCGCCCTC5960.2323577685856975No Hit
TGTAAAACGACGGCTAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC5790.22573011411261557No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGCCCCAATCCTGTCTC5710.22261121788998872No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACACCCTGGAGGAGATGAAC5510.2148139773334217No Hit
TGNAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC4680.18245542902366854No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGGG4650.18128584294018346No Hit
TGTAAAACGACGGCCAGTGCCCAAGGTTGGTGAGATGGGAACCACCCAGC4320.16842039602184788No Hit
TGTAAAACGACGGCTAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC4320.16842039602184788No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGAGCCCCCTGCCG4170.1625724656044226No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCAGGTGCCTG4110.1602332934374525No Hit
TGTAAAACGACGGCCAGTGCAGAAGATGGACTGTGGAGGGGGCTACATTA3850.15009688071391533No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGTGGGTGCCTG3820.1489272946304303No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGACCGAGTAGCCACAG3620.14113005407386325No Hit
TGTAAAACGACGGCTAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG3590.1399604679903782No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGATGTGGCTACGGGGT3500.13645170973992304No Hit
TGTAAAACGACGGCCAGTGACTGTAGGTTGGGCTCCCCCTGAGGGAGGAT3470.135282123656438No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGCACCTG3400.13255308946163952No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGTCACCTG3160.12319640079375908No Hit
TGTAAAACGACGGCTAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG3060.11929778051547558No Hit
TGTAAAACGACGGCTAGTCGGCTCTGGCTTAAGGGGTGTGGCTACGGGGT3010.11734847037633382No Hit
TGTAAAACGACGGCCAGTGACTGTAGGTTGGGCTCCCCGAGGATCCTCCC2890.1126701260423936No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG2840.11072081590325185No Hit
TGTAAAACGACGGCCAGTTGAAGAATGGTGGCTTGTTTCATGGTGGCCTT2790.10877150576411009No Hit
TGNAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCTGAGTAGCCACAG2790.10877150576411009No Hit
TGTAAAACGACGGCTAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG2780.10838164373628172No Hit
TGTAAAACGACGGCTAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC2680.10448302345799823No Hit
TGNAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCCGCGGGGGTACCG2620.1021438512910281No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGACTGCGGGGGTACCG2580.1005844031797147No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)