FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003245958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256501
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC96203.750472707708742No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT71512.787903361000542No Hit
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC56222.1918043204509923No Hit
CAGGAAANAGCTATGANNCCGTAGCCACGCCCCTATTGCAGGAAACAGCT43931.7126638882499483No Hit
CAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA42081.6405394131017035No Hit
CAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC39281.531378045309765No Hit
CAGGAAANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC37601.4658812246346018No Hit
CAGGAAANAGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT33641.3114958616145747No Hit
CAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT32681.274069106943053No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGACTGGCCGTCG31791.2393713864663296No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA31111.2128607685740016No Hit
CAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA26811.0452200966078105No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG22350.8713416321963657No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC22210.8658835638067688No Hit
CAGGAAANAGCTATGANNCCGTAGCCACACCCCTTAAGCCAGAGCCGACT22080.8608153574450002No Hit
CAGGAAANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC20100.7836226759349866No Hit
CAGGAAANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA16540.6448317940280935No Hit
CAGGAAANAGCTATGANNCCGTAGCCACGCCCCTATTGCCAGAGCCGACT16140.6292373129149594No Hit
CAGGAAANNGCTATGANNCCGTAGCCACGCCCCTATTGCAGGAAACAGCT16120.6284575888593027No Hit
CAGGAAANAGCTATGANNTAGAGGACACCCAAGGCCACCAGGGCAGCGGT15840.6175414520801089No Hit
CAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT15610.6085746254400568No Hit
CAGGAAANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC14720.5738769049633335No Hit
CAGGAAANNGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT14140.5512649073492891No Hit
CAGGAAANNGCTATGANNCTGTGAGGCACCAGAGCACCGACTGGCCGTCG13890.5415183566535803No Hit
CAGGAAANAGCTATGANNCTTGAGCTCCTCGGACACGGCAGGAAACAGCT13830.5391791844866102No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGCCTC12860.5013625677872601No Hit
NAGGANANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC11430.44561229780780587No Hit
NAGGAAANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC11280.43976436739038055No Hit
CAGGANANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC10900.42494961033290324No Hit
CAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA10640.414813197609366No Hit
CAGGAAANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA10170.39648968230143355No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG9530.37153851252041903No Hit
CAGGAAANAGCTATGANNCTTGAGCTCCTCAGCCACCACCACGTACCACT9480.36958920238127724No Hit
NAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT8890.34658734273940456No Hit
CAGGAAANNGCTATGANNCCGTAGCCACACCCCTTAAGCCAGAGCCGACT8680.33840024015500914No Hit
NAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT8600.33528134393238235No Hit
CAGGAAANAGCTATGANNATCGCACCACTGCACTCCAGCGCTCCATACAC8400.3274841033758153No Hit
CAGGAAANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC8280.3228057590418751No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG8170.31851727673576324No Hit
CAGGAAANAGCTATGANNAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG8110.3161781045687931No Hit
CAGGAAANAGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT8000.31188962226268124No Hit
CAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT7980.31110989820702456No Hit
CAGGAAANAGCTATGANNGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG7440.29005734870429356No Hit
CAGGAAANNGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG7370.2873283145094951No Hit
CAGGAAANAGCTATGANNCCGTAGCCACGCCCCTATTGTCTGACCACGCT6840.26666562703459246No Hit
CAGGAAANAGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT6660.25964811053368214No Hit
CAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT6470.25224073200494346No Hit
CAGGAAANNGCTATGANNCCGTAGCCACGCCCCTATTGCCAGAGCCGACT6270.2444434914483764No Hit
CAGGAAANAGCTATGANNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG6180.24093473319792125No Hit
NAGGANANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC6120.23859556103095117No Hit
NAGGAAANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC6090.2374259749474661No Hit
CAGGAAANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT5940.2315780445300408No Hit
CAGGANANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC5780.2253402520847872No Hit
NAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC5690.22183149383433204No Hit
NAGGAAANAGCTATGANCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC5680.2214416318065037No Hit
CAGGAAANNGCTATGANNTAGAGGACACCCAAGGCCACCAGGGCAGCGGT5670.22105176977867533No Hit
CAGGAAACAGCNATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC5650.22027204572301862No Hit
CAGGAANNNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC5620.21910245963953356No Hit
CAGGAAANAGCTATGANNATCGCACCACTGCACTCCAGCAGGAAACAGCT5570.2171531495003918No Hit
CAGGAAANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGCCTC5500.21442411530559335No Hit
CAGGAAACNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC5300.20662687474902633No Hit
CAGGAAACNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT5150.20077894433160104No Hit
CAGGAAACAGCTATGANCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC5090.19843977216463093No Hit
CAGGAANNNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT5070.19766004810897425No Hit
CAGGAAANAGCTATGANNATCGCACCACTGCGCTCCATACACCTGGGACA5070.19766004810897425No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG5030.1961005999976608No Hit
CAGGAAANAGCTATGANNGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC4960.19337156580286236No Hit
NAGGANANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA4860.18947294552457886No Hit
NAGGAAANNGCTATGANNCCGTAGCCACGCCCCTATTGCAGGAAACAGCT4850.18908308349675051No Hit
CAGGAAANAGCTATGANNGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT4730.1844047391628103No Hit
CAGGAAANNGCTATGANNCTTGAGCTCCTCGGACACGGCAGGAAACAGCT4730.1844047391628103No Hit
CAGGANANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA4720.18401487713498194No Hit
NAGGANANNGCTATGANNCCGTAGCCACGCCCCTATTGCAGGAAACAGCT4640.18089598091235512No Hit
NAGGAAANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC4620.1801162568566984No Hit
CAGGANANNGCTATGANNCCGTAGCCACGCCCCTATTGCAGGAAACAGCT4590.17894667077321336No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT4500.1754379125227582No Hit
NAGGAAANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA4450.17348860238361644No Hit
NAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT4370.17036970616098962No Hit
CAGGAAACAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT4330.1688102580496762No Hit
NAGGANANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC4320.16842039602184788No Hit
CAGGANANNGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC4110.1602332934374525No Hit
NAGGAAANNGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT3870.15087660476957204No Hit
CAGGAAANNGCTATGANNCTTGAGCTCCTCAGCCACCACCACGTACCACT3820.1489272946304303No Hit
CAGGAAACAGCNATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC3820.1489272946304303No Hit
NAGGAAANAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3810.14853743260260194No Hit
NAGGANANNGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT3770.14697798449128854No Hit
NAGGANANNGCTATGANNCTGTGAGGCACCAGAGCACCGACTGGCCGTCG3730.14541853637997515No Hit
NAGGAAANNGCTATGANNCTGTGAGGCACCAGAGCACCGACTGGCCGTCG3700.14424895029649007No Hit
CAGGAAANAGCTATGANNCCGTAGCCACCCCTTAAGCCAGAGCCGACTGG3540.13801115785123644No Hit
CAGGANANNGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT3520.13723143379557973No Hit
CAGGANANNGCTATGANNCTGTGAGGCACCAGAGCACCGACTGGCCGTCG3510.13684157176775139No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG3420.13333281351729623No Hit
NAGGANANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA3380.1317733654059828No Hit
CAGGAAACAGCNATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3340.1302139172946694No Hit
CAGGAAANNGCTATGANNATCGCACCACTGCACTCCAGCGCTCCATACAC3290.12826460715552765No Hit
CAGGAAANNGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG3260.1270950210720426No Hit
NAGGAAANAGCTATGANCACAGTGTCCCCATCAGGCTCCGGGACAGCATC3250.12670515904421426No Hit
CAGGAAANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG3240.12631529701638589No Hit
CAGGAAANNGCTATGANNAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG3220.1255355729607292No Hit
NAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA3180.1239761248494158No Hit
CAGGANANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA3050.11890791848764722No Hit
NAGGANANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA2990.11656874632067712No Hit
CAGGAAACAGCNATGANNCCGTAGCCACGCCCCTATTGCAGGAAACAGCT2970.1157890222650204No Hit
CAGGAAANNGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT2870.1118904019867369No Hit
CAGGANANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA2830.11033095387542348No Hit
NAGGAAANNGCTATGANNCCGTAGCCACACCCCTTAAGCCAGAGCCGACT2780.10838164373628172No Hit
NAGGAAANNGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA2780.10838164373628172No Hit
CAGGAAANNGCTATGANNCCGTAGCCACGCCCCTATTGTCTGACCACGCT2770.10799178170845339No Hit
NAGGAAANAGCTATGANCCCGTAGCCACGCCCCTATTGCAGGAAACAGCT2760.10760191968062502No Hit
CAGGAAACNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2740.10682219562496832No Hit
NAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2740.10682219562496832No Hit
CAGGAANNNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2610.10175398926319974No Hit
NAGGANANNGCTATGANNCCGTAGCCACACCCCTTAAGCCAGAGCCGACT2590.10097426520754305No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)