FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71947
Sequences flagged as poor quality0
Sequence length300
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAGTGCCAAGG17692.458754360848958No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA15132.1029368840952367No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTAGAGCCTGGCT13461.8708215769941763No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTGGGGTTAGGCAT11471.5942290852989007No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG10121.4065909627920552No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGACAGGGTCAGT9071.2606502008422866No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTCACCATTTGGTG8341.1591866234867334No Hit
TGTAAAACGACGGCCAGTGCCACTTCTGAAGGAGGTTCCCCCAGCAGTGC8061.120269086966795No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCCTGAGG7090.9854476211655802No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGGTGCTCTGGT7060.9812778851098726No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG6900.9590392928127649No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC6880.9562594687756266No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTGCCCAGTGTGTT6510.9048327240885652No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG6430.8937134279400114No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG6150.8547958914200731No Hit
TGTAAAACGACGGCCAGTTCCCAAAGGCTGGTGGAGGAGATGCTGAAGAT5980.8311673871043963No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA5930.8242178270115502No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGATCCCCGGTG5900.8200480909558425No Hit
TGTAAAACGACGGCCAGTCTTGCACTACATGGCCATCAGGGTCTCGTCCC5740.797809498658735No Hit
TGTAAAACGACGGCCAGTGGCTCCCCAGCTGTCTCTCTTGTAAAACGACG5730.7964195866401658No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGGTTGGAGGTG5680.7894700265473195No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCAGCACCCGGCAGC5540.7700112582873504No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC5530.7686213462687812No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCCGGCAGCGACAT5500.7644516102130735No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG5370.7463827539716736No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC5370.7463827539716736No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG5340.742213017915966No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT5280.7338735458045506No Hit
TGTAAAACGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT5120.711634953507443No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAGGAGGACCC4960.6893963612103353No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGGGCCCCGGGAGC4940.6866165371731969No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG4800.6671577689132278No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGCCAGTTAACCA4630.643529264597551No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTCCATTTGGTGTG4580.6365797045047049No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATCACGGTGGT4570.6351897924861356No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGCAGGATGGAG4480.6226805843190126No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGCCCCAATCCTGTCTC4370.6073915521147512No Hit
TGTAAAACGACGGCCAGTTGCTGCATATTATTCCATTATGCAGACAGGAC4320.600441992021905No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGTGGGAGTCTGGCTAC4320.600441992021905No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCCCTCGGAGCTG4310.5990520800033359No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA4300.5976621679847666No Hit
TGTAAAACGACGGCCAGTAGTGGAAGCTATTAGCACCAATGACAAGCAAC4130.5740336636690897No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT3850.5351161271491515No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT3830.532336303112013No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT3570.4961985906292132No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT3530.4906389425549363No Hit
TGTAAAACGACGGCCAGTAAGAGGTGAGCGAGGTCTGCGCCACGTGATCC3510.4878591185177978No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGAAGCCTCAACCC3430.47673982236924406No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA3370.4684003502578287No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGACATTCCTGCAA3350.4656205262206902No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGTGTGGCCTGAG3230.44894158199785955No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC3190.44338193392358266No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG3150.4378222858493057No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG3110.4322626377750288No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCATGTAAAACGACG3080.42809290171932113No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTCCCGACGCCAGG2990.41558369355219815No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGCTAACGGGGTT2980.4141937815336289No Hit
TGTAAAACGACGGCCAGTAGTGGAAGCTATTAGCACCAATGACAGCCTCC2960.41141395749649046No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG2910.4044643974036443No Hit
TGTAAAACGACGGCCAGTTCCTTCTTGGGTGGGTATTTTGCAACTGGGTA2820.39195518923652134No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC2810.39056527721795214No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT2800.3891753651993829No Hit
TGTAAAACGACGGCCAGTGCCATCGGCTCCCAAGTTTTACATCCTGGAGA2780.3863955411622444No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCGTGACCGAGAGCT2760.383615717125106No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA2740.38083589308796756No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT2710.37666615703225986No Hit
TGTAAAACGACGGCCAGTTCTTTGGCTCTACCTAAAAGTTGGTCAGCAAA2620.36415694886513683No Hit
TGTAAAACGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCAGGTGTA2450.34052844454946No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGCCAGCCCCCCAC2440.3391385325308908No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAATTAGTAGT2390.3321889724380447No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTAGGGTTGGGTCA2310.32106967628949085No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAGGAGAGTGA2280.3168999402337832No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGCATCCTGGGTGAC2190.3043907320666602No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTCCTCACTCGGAT2100.29188152389953714No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGGATGCTGAGTGGGAG2060.28632187582526025No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCCCACAGACCCC2040.2835420517881218No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA2020.28076222775098336No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCTGCAGACAGGAT1970.2738126676581373No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCACCTTGGCCTCC1950.2710328436209988No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTGGTGGAGTGTGT1920.26686310756529114No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT1900.2640832835281527No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGTGAACGAAG1850.25713372343530655No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCAATAATGCTGCT1840.2557438114167373No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCACTAGGAGAG1840.2557438114167373No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCAGTTTCCTGCC1810.25157407536102966No Hit
TGTAAAACGACGGCCAGTCCTGGCCCACAAACTGTGCTGGGGTCATAGCT1740.24184469123104507No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCCCTCAGAGCTG1740.24184469123104507No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC1730.24045477921247585No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGCTCTGGTG1720.23906486719390665No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGGTGACAGAGTGTG1720.23906486719390665No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTGATAAGGAAGGA1660.23072539508249126No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACCTGGCGCAGGC1650.22933548306392207No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCACAGTGACCTGT1640.22794557104535282No Hit
TGTAAAACGACGGCCAGTCTTGCACTACATGGCCATCACCCCCTCTCCAC1590.2209960109525067No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAAGGAGAGTG1570.21821618691536823No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACACTGCCGAGTG1560.21682627489679904No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGGGGGCGAGTGT1530.21265653884109137No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACACGGCAGGAGA1500.2084868027853837No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTGCGGCCCCTGA1500.2084868027853837No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG1480.20570697874824523No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCAGGATGGAGGG1470.20431706672967598No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCGGACCGAGAGCTC1430.19875741865539912No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGCCTTCGGAGGC1420.19736750663682986No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG1400.19458768259969145No Hit
TGTAAAACGACGGCCAGTAGATCATCCAGCGCACACGGCGTCTTGGCGGG1390.1931977705811222No Hit
TGTAAAACGACGGCCAGTAGTGGAAGCTATTAGCACCAATGACACTCCCC1390.1931977705811222No Hit
TGTAAAACGACGGCCAGTGCCACTTCTGAAGGAGGTTCCCCCAGTAGTGC1360.18902803452541453No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG1340.18624821048827608No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGTAGAGATGGGGT1330.1848582984697069No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA1320.18346838645113764No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCATAGCTGTT1320.18346838645113764No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCACTTCCTGC1310.18207847443256842No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGTGTAAAACGACG1300.1806885624139992No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGCACATTGGAGT1250.17373900232115308No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA1230.1709591782840146No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGGATGGTGTGGC1230.1709591782840146No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTGCCAGTACCGGG1220.1695692662654454No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGGGTCAGTGGGAGTCT1220.1695692662654454No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTACAGGAACCATGG1210.1681793542468762No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTTGGACCCAGAG1190.16539953020973772No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTGTAAAACGACGG1180.1640096181911685No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTGTACCGGGGCCA1180.1640096181911685No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCCCCCAGGATGG1170.16261970617259927No Hit
TGTAAAACGACGGCCAGTTGGAGTGCCTGGGTTAGAACCCTGCTGGTACC1150.15983988213546083No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGTTCGGAGGCAGG1130.15706005809832238No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGATACGACTCACT1120.15567014607975316No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC1110.15428023406118394No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCCCGGCAGCGACA1100.1528903220426147No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGTGGGGTCACCCC1060.14733067396833782No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGGTGTGGCCTGA1060.14733067396833782No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAAGCCCCAGACCC1050.14594076194976857No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA1030.14316093791263013No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTTGCTGCGGCCC1020.1417710258940609No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCCGAGTAGCTGAG1020.1417710258940609No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTGGGTGGGGTTAG1020.1417710258940609No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCGGTGCCCCGGCAG1010.14038111387549168No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCTGTGGGAGACCCAGC1000.13899120185692246No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCAGGTGACTCCAG980.136211377819784No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGAAAGCCTCAGGCA970.13482146580121476No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC970.13482146580121476No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG950.13204164176407635No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCACCGAGGCAG920.12787190570836865No Hit
TGTAAAACGACGGCCAGTGGCTCCCCAGCTGTCTCTCTTCAGCTCTATAT920.12787190570836865No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTACGGCCAGTACC900.1250920816712302No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCTCCTCAGTCACC850.11814252157838409No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTTGTAAAACGAC850.11814252157838409No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGTGTAAAACGACG840.11675260955981487No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCCGAATGCTGCTG840.11675260955981487No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAGTAGGAGAG840.11675260955981487No Hit
TGTAAAACGACGGCCAGTCTCTCACCCACCTCCACCCACTGGAGGTCATA830.11536269754124563No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGCTGCACGAGACCC830.11536269754124563No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG820.11397278552267641No Hit
TGTAAAACGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT810.1125828735041072No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGGACACCAGTGA780.10841313744839952No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACTGCCTTCCAGCATTT770.10702322542983028No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGCAATCCC770.10702322542983028No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGCCTGGGTGGGA770.10702322542983028No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC760.10563331341126106No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGTAAAACGACGGC740.10285348937412261No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGGGGGTGCCTCAA730.10146357735555339No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTAGGAACCATGGCT720.10007366533698417No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)