FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71947
Sequences flagged as poor quality0
Sequence length300
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGCACTCCTTC19202.668631075652911No Hit
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG15112.1001570600580983No Hit
CAGGAAACAGCTATGACCGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC14692.041780755278191No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT14071.955606210126899No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCAGCACAGGGAGT12461.7318303751372537No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACCCTGTCTCTCTAGC9841.3676734262721169No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT9771.3579440421421325No Hit
CAGGAAACAGCTATGACCTGACTGAAAGGCTTTTCCACGTGCACTGCTGG9721.3509944820492863No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATGGTGAGCCCTGC8661.2036638080809485No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTCAGGAATCATGC7631.0605028701683183No Hit
CAGGAAACAGCTATGACCGCAGCCACCCTGAACACACTGGGCAGGAAGAA7531.0466037499826262No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCCTCCCCACCTCC7431.0327046297969338No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC7040.9784980610727341No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT6490.9020529000514267No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCGGGGATCCTCG6350.8825941317914575No Hit
CAGGAAACAGCTATGACCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC6320.8784243957357499No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCCCGTTAGCTGC6150.8547958914200731No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC6080.8450665072900885No Hit
CAGGAAACAGCTATGACCAGAGGCACAGGTGGAGAGGGACGAGACCCTGA5980.8311673871043963No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCTCTCCACGTGC5980.8311673871043963No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGTGCTGCCTCTCCA5810.8075388827887194No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC5800.8061489707701504No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT5800.8061489707701504No Hit
CAGGAAACAGCTATGACCTCCCTTAAACTTGGGATTGCTCCATTGCACTC5520.767231434250212No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGCCCGGACGAGGA5300.736653369841689No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGGTCCTCCTC5280.7338735458045506No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA5250.729703809748843No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAACTGGCCAGCCTG5250.729703809748843No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA5050.7019055693774584No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA4980.6921761852474738No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG4900.68105688909892No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACCACCGTGATCTCTC4890.6796669770803507No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATGGAGCCCTGCAG4870.6768871530432123No Hit
CAGGAAACAGCTATGACCTCTACGCCCCTCAGCTGTGTCCTGTCTGCATA4680.6504788246903971No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCTGGGTGCTGGTG4510.6268503203747203No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCAGCTCCGA4470.6212906723004434No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTGGCAGCTGCAC4420.6143411122075972No Hit
CAGGAAACAGCTATGACCCCCATCCACTAGTCGGGGAGTTGCTTGTCATT4290.5962722559661973No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT4150.5768134877062282No Hit
CAGGAAACAGCTATGACCGGCGGGCCCTGATATAGAGCCACTGGCCGTCG4150.5768134877062282No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGGCACGGGAACA3940.5476253353162746No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA3910.5434555992605669No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT3890.5406757752234284No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT3880.539285863204859No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCTTCCCATCTCA3880.539285863204859No Hit
CAGGAAACAGCTATGACCGTCTCAGGGATCACGTGGCGCAGACCTCGCTC3700.5142674468706131No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAGGCACTACGT3680.5114876228334746No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACTGCCTCCCGACT3440.4781297343878133No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT3390.4711801742949671No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC3370.4684003502578287No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC3330.4628407021835518No Hit
CAGGAAACAGCTATGACCCCCATCCACTAGTCGGGGAGTAGGAGGCTGTC3310.4600608781464133No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC3230.44894158199785955No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG3230.44894158199785955No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC3190.44338193392358266No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACCTCCAACCCCGT3160.43921219786787496No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCAGGCCCTGGCGT3090.4294828137378904No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTCCCCAACCATAG3050.4239231656636135No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCACGGGCATCCTG2990.41558369355219815No Hit
CAGGAAACAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT2950.41002404547792126No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT2930.4072442214407828No Hit
CAGGAAACAGCTATGACCCCTGACCCCTTCTTCTCCAGGATGTAAAACTT2850.396124925292229No Hit
CAGGAAACAGCTATGACCAGGTTGCTGGATTCAGTTTGCTGACCAACTTT2780.3863955411622444No Hit
CAGGAAACAGCTATGACCCCAGCAGAGCTCTTCTTACAGAGGCAGGAAAC2750.38222580510653675No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTGGCATGAAGGA2720.37805606905082906No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGACAGGAAACAGCT2680.37249642097655217No Hit
CAGGAAACAGCTATGACCGTCAACAGCATACCTGTACACCTGAGTGTTCC2630.365546860883706No Hit
CAGGAAACAGCTATGACCTGGAGTTTGAAACAGCACCTTGGATACTACTA2630.365546860883706No Hit
CAGGAAACAGCTATGACCAAAGGCAGCAGGGTCGATGGTGGGGGGCTGGC2610.36276703684656764No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCTCTGCAGCTAGC2460.3419183565680292No Hit
CAGGAAACAGCTATGACCGAGGCGCAGGTCACAGTGAGACCAATACAGAG2440.3391385325308908No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA2430.3377486205123216No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCACTGGCCGTCGTT2340.32523941234519854No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG2300.31967976427092165No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATCCGAGTGAGGAG2290.31828985225235246No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACAGGAAACAGCTA2290.31828985225235246No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGTCTGTGGGCGGA2280.3168999402337832No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGTCTGCAGCCTGG2240.3113402921595063No Hit
CAGGAAACAGCTATGACCGCAGCCACCCTGAACACACTCCACCAGGAAGA2240.3113402921595063No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA2220.30856046812236787No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGCATCCTGG2190.3043907320666602No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGATGCCACG2180.3030008200480909No Hit
CAGGAAACAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT2170.30161090802952173No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTATTGAGGCACTT2070.2877117878438295No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTAGTGGCATGAA1960.272422755639568No Hit
CAGGAAACAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG1930.26825301958386033No Hit
CAGGAAACAGCTATGACCTCCTGCCCCACCCTGATACAGGTCACTGTGGA1840.2557438114167373No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACTCGCCCCCTGCC1840.2557438114167373No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCACGCCCGGCCT1810.25157407536102966No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCACAGGGACGCAG1800.2501841633424604No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACCCTTTGTCCA1790.24879425132389119No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCAGCTCTGA1740.24184469123104507No Hit
CAGGAAACAGCTATGACCCCGTAGCCACGCCCCTATTGTCTGACCACGCT1730.24045477921247585No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGAAAAGTTTCAGG1710.2376749551753374No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTTGGCATGAAGG1690.23489513113819893No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCTCCCCACCTCCT1660.23072539508249126No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCCTGCCCAGGCC1630.2265556590267836No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCCTGGCTTGTC1610.22377583498964515No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCGTGTTCTCACTC1590.2209960109525067No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAACTGGCCGTCGTT1560.21682627489679904No Hit
CAGGAAACAGCTATGACCTCCAGTGGTGGGGCTCAACACAGGAAACAGCT1560.21682627489679904No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCCGGGCATCCTGG1560.21682627489679904No Hit
CAGGAAACAGCTATGACCAGAGGCACAGGTGGAGAGGGGGTGATGGCCAT1550.21543636287822981No Hit
CAGGAAACAGCTATGACCTGACTGAAAGGCTTTTCCACGTGCACTACTGG1550.21543636287822981No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCAGCAGTAATGGA1540.21404645085966056No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAGGCCGCAGCTCC1520.21126662682252212No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCGGTACTGGCCGT1510.2098767148039529No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCTCCCCGCCAAGA1490.20709689076681445No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAAACCCCATCTCTA1470.20431706672967598No Hit
CAGGAAACAGCTATGACCGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG1430.19875741865539912No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGACCGGGATTCGTG1420.19736750663682986No Hit
CAGGAAACAGCTATGACCCCCATCCACTAGTCGGGGAGTGTCATTGGTGC1400.19458768259969145No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGGGGGCCTGGCT1400.19458768259969145No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACTCTTGGCCTGCT1390.1931977705811222No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC1350.1876381225068453No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACCATCCTGCCTCC1350.1876381225068453No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCCGTCCTCCCAGC1300.1806885624139992No Hit
CAGGAAACAGCTATGACCTCCTGGAACCTGCCTCCGAACGCAGCTCCCCA1300.1806885624139992No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCGGTACTGGCAGT1300.1806885624139992No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGACAGGGCTGACTG1270.17651882635829153No Hit
CAGGAAACAGCTATGACCACCCAGTTAGAGCCATGGTTCCTGTAGGCTAG1270.17651882635829153No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGGCCTCTCCACGTG1270.17651882635829153No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC1240.17234909030258383No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTGACCCTGGCAG1220.1695692662654454No Hit
CAGGAAACAGCTATGACCGTGCAGTCCTCAGGCCACACCTGCCTCCCGAC1200.16678944222830694No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1200.16678944222830694No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCGGTACAGTAACT1190.16539953020973772No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA1170.16261970617259927No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGTGAGTCGTAT1160.16122979415403005Clontech Universal Primer Mix Long (95% over 22bp)
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCCCACAGAGAGCAC1160.16122979415403005No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCAGCAACAGGGAC1160.16122979415403005No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGACAAGTGCATGG1130.15706005809832238No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCAGGGAACAGCT1100.1528903220426147No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTAGTCTC1100.1528903220426147No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCACCCAGCACAGG1100.1528903220426147No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAACCAGTGAC1090.15150041002404546No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTAGGGTCTGGGGC1080.15011049800547624No Hit
CAGGAAACAGCTATGACCGGCGGGCCCTGATATAGAGCTGAAGAGAGACA1080.15011049800547624No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGGCACCGCCTCTCC1050.14594076194976857No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG1040.14455084993119935No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC1030.14316093791263013No Hit
CAGGAAACAGCTATGACCTGCCAAAATTAAGGATGCACACCTGGAGTCAC990.13760128983835324No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT980.136211377819784No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTACTGGCATGAA940.1306517297455071No Hit
CAGGAAACAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG940.1306517297455071No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGAGAG920.12787190570836865No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGAGGAGGGTGG900.1250920816712302No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTGTAATCCCAGC870.12092234561552254No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCCTGGCTTGTCC860.11953243359695333No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGACTGGCCGTCGTT860.11953243359695333No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTCGGAGGCACTTA860.11953243359695333No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCTCACTGGTGTC840.11675260955981487No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTACATCAAATGC840.11675260955981487No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCACCCCCCCATC790.10980304946696874No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT780.10841313744839952No Hit
CAGGAAACAGCTATGACCGCAGCCACCCTGAACACACTCCACCCTACTAC780.10841313744839952No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCCTCTCCCAACT770.10702322542983028No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCCAGAGAGCACCCG770.10702322542983028No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCAGACACACGGC770.10702322542983028No Hit
CAGGAAACAGCTATGACCACCCAGTTAGAGCCATGGTTCCTAGGCTAGCA770.10702322542983028No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATCCCAGCAGCAGA750.10424340139269185No Hit
CAGGAAACAGCTATGACCTCCCTTAAACTTGGGATTGCTCCAGCCTGTGC750.10424340139269185No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCGGTACTGGCCGA750.10424340139269185No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGTCTGTGTGCGGA740.10285348937412261No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATGAGTTAGCCAGC730.10146357735555339No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGTGGACTAGGTCC730.10146357735555339No Hit
CAGGAAACAGCTATGACCAGGTCCAGATGGCTGGTGACTCACGCCTCTGA720.10007366533698417No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)